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P49326 (FMO5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 5

EC=1.14.13.8
Alternative name(s):
Dimethylaniline oxidase 5
Hepatic flavin-containing monooxygenase 5
Short name=FMO 5
Gene names
Name:FMO5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme.

Catalytic activity

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.

Cofactor

FAD.

Subcellular location

Microsome membrane. Endoplasmic reticulum membrane.

Tissue specificity

Expressed in fetal and adult liver.

Sequence similarities

Belongs to the FMO family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P49326-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P49326-2)

The sequence of this isoform differs from the canonical sequence as follows:
     278-285: ALSQHPTL → SKDIALTE
     286-533: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: P49326-3)

The sequence of this isoform differs from the canonical sequence as follows:
     419-464: RYVESQRHTI...TDPKLALHLL → SLTMRKTSDK...CPGIKGPKET
     465-533: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Dimethylaniline monooxygenase [N-oxide-forming] 5
PRO_0000147665

Regions

Nucleotide binding10 – 156FAD Potential
Nucleotide binding192 – 1976NADP By similarity

Amino acid modifications

Modified residue51Omega-N-methylated arginine By similarity

Natural variations

Alternative sequence278 – 2858ALSQHPTL → SKDIALTE in isoform 2.
VSP_042729
Alternative sequence286 – 533248Missing in isoform 2.
VSP_042730
Alternative sequence419 – 46446RYVES…ALHLL → SLTMRKTSDKPKLKNILQIP DYLKTVKIINKESLRNCPGI KGPKET in isoform 3.
VSP_045616
Alternative sequence465 – 53369Missing in isoform 3.
VSP_045617
Natural variant4001P → A. Ref.3
Corresponds to variant rs28381218 [ dbSNP | Ensembl ].
VAR_022308
Natural variant4571P → L. Ref.9
VAR_015370
Natural variant5061R → S. Ref.3
Corresponds to variant rs28381223 [ dbSNP | Ensembl ].
VAR_022309

Experimental info

Sequence conflict3511S → P in CAA87633. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F9D9F092F1DD71DA

FASTA53360,221
        10         20         30         40         50         60 
MTKKRIAVIG GGVSGLSSIK CCVEEGLEPV CFERTDDIGG LWRFQENPEE GRASIYKSVI 

        70         80         90        100        110        120 
INTSKEMMCF SDYPIPDHYP NFMHNAQVLE YFRMYAKEFD LLKYIRFKTT VCSVKKQPDF 

       130        140        150        160        170        180 
ATSGQWEVVT ESEGKKEMNV FDGVMVCTGH HTNAHLPLES FPGIEKFKGQ YFHSRDYKNP 

       190        200        210        220        230        240 
EGFTGKRVII IGIGNSGGDL AVEISQTAKQ VFLSTRRGAW ILNRVGDYGY PADVLFSSRL 

       250        260        270        280        290        300 
THFIWKICGQ SLANKYLEKK INQRFDHEMF GLKPKHRALS QHPTLNDDLP NRIISGLVKV 

       310        320        330        340        350        360 
KGNVKEFTET AAIFEDGSRE DDIDAVIFAT GYSFDFPFLE DSVKVVKNKI SLYKKVFPPN 

       370        380        390        400        410        420 
LERPTLAIIG LIQPLGAIMP ISELQGRWAT QVFKGLKTLP SQSEMMAEIS KAQEEIDKRY 

       430        440        450        460        470        480 
VESQRHTIQG DYIDTMEELA DLVGVRPNLL SLAFTDPKLA LHLLLGPCTP IHYRVQGPGK 

       490        500        510        520        530 
WDGARKAILT TDDRIRKPLM TRVVERSSSM TSTMTIGKFM LALAFFAIII AYF 

« Hide

Isoform 2 [UniParc].

Checksum: 4DE507F329B99046
Show »

FASTA28532,480
Isoform 3 [UniParc].

Checksum: 1989AA851C48E893
Show »

FASTA46452,477

References

« Hide 'large scale' references
[1]"Characterization of flavin-containing monooxygenase 5 (FMO5) cloned from human and guinea pig: evidence that the unique catalytic properties of FMO5 are not confined to the rabbit ortholog."
Overby L.H., Buckpitt A.R., Lawton M.P., Atta-Asafo-Adjei E., Schulze J., Philpot R.M.
Arch. Biochem. Biophys. 317:275-284(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver.
[2]"Cloning, primary sequence and chromosomal localisation of human flavin-containing monooxygenase 5 (FMO5)."
Dolphin C.T., Povey S., Shephard E.A., Smith R.L., Phillips I.R.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver and Placenta.
[3]NIEHS SNPs program
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-400 AND SER-506.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Trachea.
[5]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[6]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[9]"Identification of novel variants of the flavin-containing monooxygenase gene family in African Americans."
Furnes B., Feng J., Sommer S.S., Schlenk D.
Drug Metab. Dispos. 31:187-193(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT LEU-457.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L37080 mRNA. Translation: AAA67849.1.
Z47553 mRNA. Translation: CAA87633.1.
AY902236 Genomic DNA. Translation: AAW69390.1.
AK314647 mRNA. Translation: BAG37208.1.
AK225739 mRNA. No translation available.
AL356378 Genomic DNA. Translation: CAH72648.1.
AL356378 Genomic DNA. Translation: CAH72649.1.
CH471223 Genomic DNA. Translation: EAW50939.1.
CH471223 Genomic DNA. Translation: EAW50940.1.
BC035687 mRNA. Translation: AAH35687.1.
PIRS51131.
S71618.
RefSeqNP_001138301.1. NM_001144829.2.
NP_001138302.1. NM_001144830.2.
NP_001452.2. NM_001461.3.
XP_005273003.1. XM_005272946.2.
XP_005273004.1. XM_005272947.2.
XP_005273005.1. XM_005272948.2.
UniGeneHs.642706.

3D structure databases

ProteinModelPortalP49326.
SMRP49326. Positions 1-489.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000254090.

PTM databases

PhosphoSiteP49326.

Polymorphism databases

DMDM1346021.

Proteomic databases

PaxDbP49326.
PRIDEP49326.

Protocols and materials databases

DNASU2330.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000254090; ENSP00000254090; ENSG00000131781. [P49326-1]
ENST00000369272; ENSP00000358277; ENSG00000131781. [P49326-2]
ENST00000441068; ENSP00000416011; ENSG00000131781. [P49326-3]
GeneID2330.
KEGGhsa:2330.
UCSCuc001epi.2. human. [P49326-1]
uc001epj.2. human. [P49326-2]

Organism-specific databases

CTD2330.
GeneCardsGC01M146655.
HGNCHGNC:3773. FMO5.
HPAHPA012373.
MIM603957. gene.
neXtProtNX_P49326.
PharmGKBPA28189.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2072.
HOGENOMHOG000076537.
HOVERGENHBG002037.
InParanoidP49326.
KOK00485.
OMAMTKKRIA.
PhylomeDBP49326.
TreeFamTF105285.

Gene expression databases

ArrayExpressP49326.
BgeeP49326.
CleanExHS_FMO5.
GenevestigatorP49326.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR012143. DiMe-aniline_mOase.
IPR000960. Flavin_mOase.
IPR020946. Flavin_mOase-like.
IPR002257. Flavin_mOase_5.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00743. FMO-like. 1 hit.
[Graphical view]
PIRSFPIRSF000332. FMO. 1 hit.
PRINTSPR00370. FMOXYGENASE.
PR01125. FMOXYGENASE5.
ProtoNetSearch...

Other

ChiTaRSFMO5. human.
GeneWikiFMO5.
GenomeRNAi2330.
NextBio9457.
PROP49326.
SOURCESearch...

Entry information

Entry nameFMO5_HUMAN
AccessionPrimary (citable) accession number: P49326
Secondary accession number(s): B2RBG1, C9JJD1, Q8IV22
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM