P49326 (FMO5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 5
EC=1.14.13.8

Alternative name(s):
Dimethylaniline oxidase 5
Hepatic flavin-containing monooxygenase 5
Short name=FMO 5

Gene names

Name:

FMO5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD.
Subcellular location	Microsome membrane. Endoplasmic reticulum membrane.
Tissue specificity	Expressed in fetal and adult liver.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellular membrane-bounded organelle Non-traceable author statement. Source: UniProtKB intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	N,N-dimethylaniline monooxygenase activity Non-traceable author statement. Source: UniProtKB NADP binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P49326-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P49326-2)

The sequence of this isoform differs from the canonical sequence as follows:
     278-285: ALSQHPTL → SKDIALTE
     286-533: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: P49326-3)

The sequence of this isoform differs from the canonical sequence as follows:
     419-464: RYVESQRHTI...TDPKLALHLL → SLTMRKTSDK...CPGIKGPKET
     465-533: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Dimethylaniline monooxygenase [N-oxide-forming] 5		PRO_0000147665
Regions
	Nucleotide binding	10 – 15	6	FAD Potential
	Nucleotide binding	192 – 197	6	NADP By similarity
Amino acid modifications
	Modified residue	5	1	Omega-N-methylated arginine By similarity
Natural variations
	Alternative sequence	278 – 285	8	ALSQHPTL → SKDIALTE in isoform 2.		VSP_042729
	Alternative sequence	286 – 533	248	Missing in isoform 2.		VSP_042730
	Alternative sequence	419 – 464	46	RYVES…ALHLL → SLTMRKTSDKPKLKNILQIP DYLKTVKIINKESLRNCPGI KGPKET in isoform 3.		VSP_045616
	Alternative sequence	465 – 533	69	Missing in isoform 3.		VSP_045617
	Natural variant	400	1	P → A. Ref.3 Corresponds to variant rs28381218 [ dbSNP | Ensembl ].		VAR_022308
	Natural variant	457	1	P → L. Ref.9		VAR_015370
	Natural variant	506	1	R → S. Ref.3 Corresponds to variant rs28381223 [ dbSNP | Ensembl ].		VAR_022309
Experimental info
	Sequence conflict	351	1	S → P in CAA87633. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F9D9F092F1DD71DA	FASTA	533	60,221
	10 20 30 40 50 60 MTKKRIAVIG GGVSGLSSIK CCVEEGLEPV CFERTDDIGG LWRFQENPEE GRASIYKSVI 70 80 90 100 110 120 INTSKEMMCF SDYPIPDHYP NFMHNAQVLE YFRMYAKEFD LLKYIRFKTT VCSVKKQPDF 130 140 150 160 170 180 ATSGQWEVVT ESEGKKEMNV FDGVMVCTGH HTNAHLPLES FPGIEKFKGQ YFHSRDYKNP 190 200 210 220 230 240 EGFTGKRVII IGIGNSGGDL AVEISQTAKQ VFLSTRRGAW ILNRVGDYGY PADVLFSSRL 250 260 270 280 290 300 THFIWKICGQ SLANKYLEKK INQRFDHEMF GLKPKHRALS QHPTLNDDLP NRIISGLVKV 310 320 330 340 350 360 KGNVKEFTET AAIFEDGSRE DDIDAVIFAT GYSFDFPFLE DSVKVVKNKI SLYKKVFPPN 370 380 390 400 410 420 LERPTLAIIG LIQPLGAIMP ISELQGRWAT QVFKGLKTLP SQSEMMAEIS KAQEEIDKRY 430 440 450 460 470 480 VESQRHTIQG DYIDTMEELA DLVGVRPNLL SLAFTDPKLA LHLLLGPCTP IHYRVQGPGK 490 500 510 520 530 WDGARKAILT TDDRIRKPLM TRVVERSSSM TSTMTIGKFM LALAFFAIII AYF
	Isoform 2 [UniParc]. Checksum: 4DE507F329B99046	FASTA	285	32,480
	10 20 30 40 50 60 MTKKRIAVIG GGVSGLSSIK CCVEEGLEPV CFERTDDIGG LWRFQENPEE GRASIYKSVI 70 80 90 100 110 120 INTSKEMMCF SDYPIPDHYP NFMHNAQVLE YFRMYAKEFD LLKYIRFKTT VCSVKKQPDF 130 140 150 160 170 180 ATSGQWEVVT ESEGKKEMNV FDGVMVCTGH HTNAHLPLES FPGIEKFKGQ YFHSRDYKNP 190 200 210 220 230 240 EGFTGKRVII IGIGNSGGDL AVEISQTAKQ VFLSTRRGAW ILNRVGDYGY PADVLFSSRL 250 260 270 280 THFIWKICGQ SLANKYLEKK INQRFDHEMF GLKPKHRSKD IALTE
	Isoform 3 [UniParc]. Checksum: 1989AA851C48E893	FASTA	464	52,477
	10 20 30 40 50 60 MTKKRIAVIG GGVSGLSSIK CCVEEGLEPV CFERTDDIGG LWRFQENPEE GRASIYKSVI 70 80 90 100 110 120 INTSKEMMCF SDYPIPDHYP NFMHNAQVLE YFRMYAKEFD LLKYIRFKTT VCSVKKQPDF 130 140 150 160 170 180 ATSGQWEVVT ESEGKKEMNV FDGVMVCTGH HTNAHLPLES FPGIEKFKGQ YFHSRDYKNP 190 200 210 220 230 240 EGFTGKRVII IGIGNSGGDL AVEISQTAKQ VFLSTRRGAW ILNRVGDYGY PADVLFSSRL 250 260 270 280 290 300 THFIWKICGQ SLANKYLEKK INQRFDHEMF GLKPKHRALS QHPTLNDDLP NRIISGLVKV 310 320 330 340 350 360 KGNVKEFTET AAIFEDGSRE DDIDAVIFAT GYSFDFPFLE DSVKVVKNKI SLYKKVFPPN 370 380 390 400 410 420 LERPTLAIIG LIQPLGAIMP ISELQGRWAT QVFKGLKTLP SQSEMMAEIS KAQEEIDKSL 430 440 450 460 TMRKTSDKPK LKNILQIPDY LKTVKIINKE SLRNCPGIKG PKET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of flavin-containing monooxygenase 5 (FMO5) cloned from human and guinea pig: evidence that the unique catalytic properties of FMO5 are not confined to the rabbit ortholog." Overby L.H., Buckpitt A.R., Lawton M.P., Atta-Asafo-Adjei E., Schulze J., Philpot R.M. Arch. Biochem. Biophys. 317:275-284(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[2]	"Cloning, primary sequence and chromosomal localisation of human flavin-containing monooxygenase 5 (FMO5)." Dolphin C.T., Povey S., Shephard E.A., Smith R.L., Phillips I.R. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver and Placenta.
[3]	NIEHS SNPs program Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-400 AND SER-506.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Trachea.
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[9]	"Identification of novel variants of the flavin-containing monooxygenase gene family in African Americans." Furnes B., Feng J., Sommer S.S., Schlenk D. Drug Metab. Dispos. 31:187-193(2003) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LEU-457.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	L37080 mRNA. Translation: AAA67849.1. Z47553 mRNA. Translation: CAA87633.1. AY902236 Genomic DNA. Translation: AAW69390.1. AK314647 mRNA. Translation: BAG37208.1. AK225739 mRNA. No translation available. AL356378 Genomic DNA. Translation: CAH72648.1. AL356378 Genomic DNA. Translation: CAH72649.1. CH471223 Genomic DNA. Translation: EAW50939.1. CH471223 Genomic DNA. Translation: EAW50940.1. BC035687 mRNA. Translation: AAH35687.1.
IPI	IPI00215760. IPI00643673. IPI00921588.
PIR	S51131. S71618.
RefSeq	NP_001138301.1. NM_001144829.1. NP_001138302.1. NM_001144830.1. NP_001452.2. NM_001461.2. XP_003960188.1. XM_003960139.1. XP_003960189.1. XM_003960140.1. XP_003960190.1. XM_003960141.1. XP_003960191.1. XM_003960142.1. XP_003960192.1. XM_003960143.1. XP_003960193.1. XM_003960144.1. XP_003960194.1. XM_003960145.1. XP_003960195.1. XM_003960146.1.
UniGene	Hs.642706.
3D structure databases
ProteinModelPortal	P49326.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000254090.
PTM databases
PhosphoSite	P49326.
Polymorphism databases
DMDM	1346021.
Proteomic databases
PaxDb	P49326.
PRIDE	P49326.
Protocols and materials databases
DNASU	2330.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000254090; ENSP00000254090; ENSG00000131781. ENST00000369272; ENSP00000358277; ENSG00000131781. ENST00000441068; ENSP00000416011; ENSG00000131781.
GeneID	101060372. 2330.
KEGG	hsa:101060372. hsa:2330.
UCSC	uc001epi.2. human.
Organism-specific databases
CTD	2330.
GeneCards	GC01M146655.
HGNC	HGNC:3773. FMO5.
HPA	HPA012373.
MIM	603957. gene.
neXtProt	NX_P49326.
PharmGKB	PA28189.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2072.
HOGENOM	HOG000076537.
HOVERGEN	HBG002037.
InParanoid	P49326.
KO	K00485.
OMA	PIHYRLQ.
OrthoDB	EOG48SGT0.
PhylomeDB	P49326.
Gene expression databases
ArrayExpress	P49326.
Bgee	P49326.
CleanEx	HS_FMO5.
Genevestigator	P49326.
GermOnline	ENSG00000131781. Homo sapiens.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR012143. DiMe-aniline_mOase. IPR000960. Flavin_mOase. IPR020946. Flavin_mOase-like. IPR002257. Flavin_mOase_5. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01125. FMOXYGENASE5.
ProtoNet	Search...
Other
ChiTaRS	FMO5. human.
NextBio	9457.
SOURCE	Search...

Entry information

Entry name

FMO5_HUMAN

Accession

Primary (citable) accession number: P49326
Secondary accession number(s): B2RBG1, C9JJD1, Q8IV22

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.