Reviewed, UniProtKB/Swiss-Prot P49323 (PRXC_STRLI)

Names and origin

Protein names

Recommended name:
    Non-heme chloroperoxidase
    EC=1.11.1.10
Alternative name(s):
    Chloride peroxidase
    Chloroperoxidase L
      Short name=CPO-L

Gene names

Name:	cpo
Synonyms:	cpoL

Organism

Streptomyces lividans

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	RH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the bacterial non-heme bromo- and chloro-peroxidases family.

Ontologies

Keywords
Ligand	Chloride
Molecular function	Oxidoreductase Peroxidase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW chloride peroxidase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 276

275

Non-heme chloroperoxidase

PRO_0000207063

Sites

Active site

97

1

By similarity

Active site

227

1

By similarity

Active site

256

1

By similarity

Secondary structure

1

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276

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Beta strand

11 – 18

8

Beta strand

24 – 28

5

Helix

35 – 38

4

Helix

39 – 47

9

Beta strand

51 – 55

5

Helix

72 – 86

15

Beta strand

90 – 96

7

Helix

99 – 109

11

Beta strand

114 – 122

9

Helix

140 – 152

13

Helix

154 – 163

10

Turn

164 – 170

7

Helix

178 – 190

13

Helix

193 – 205

13

Helix

209 – 214

6

Beta strand

219 – 224

6

Beta strand

228 – 230

3

Turn

233 – 235

3

Helix

236 – 242

7

Beta strand

246 – 251

6

Helix

258 – 261

4

Helix

263 – 275

13

Sequences

	Sequence		Length	Mass (Da)
	P49323-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CF8977B0C1FA53B0	FASTA	276	29,914
	10 20 30 40 50 60 MGTVTTSDGT NIFYKDWGPR DGLPVVFHHG WPLSADDWDN QMLFFLSHGY RVIAHDRRGH 70 80 90 100 110 120 GRSDQPSTGH DMDTYAADVA ALTEALDLRG AVHIGHSTGG GEVARYVARA EPGRVAKAVL 130 140 150 160 170 180 VSAVPPVMVK SDTNPDGLPL EVFDEFRAAL AANRAQFYID VPSGPFYGFN REGATVSQGL 190 200 210 220 230 240 IDHWWLQGMM GAANAHYECI AAFSETDFTD DLKRIDVPVL VAHGTDDQVV PYADAAPKSA 250 260 270 ELLANATLKS YEGLPHGMLS THPEVLNPDL LAFVKS

References

[1]	"Chloroperoxidase from Streptomyces lividans: isolation and characterization of the enzyme and the corresponding gene." Bantleon R., Altenbuchner J., van Pee K.-H. J. Bacteriol. 176:2339-2347(1994) [PubMed: 8157602] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21. Strain: TK64.
[2]	"Structural investigation of the cofactor-free chloroperoxidases." Hofmann B., Tolzer S., Pelletier I., Altenbuchner J., van Pee K.-H., Hecht H.-J. J. Mol. Biol. 279:889-900(1998) [PubMed: 9642069] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). Strain: TK64.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U02635 Unassigned DNA. Translation: AAA18642.1.

PIR

A55211.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A88	X-ray	1.90	A/B/C	1-276	[»]

ModBase

Search...

Protein family/group databases

PeroxiBase

5557. SliHalNPrx.

Family and domain databases

InterPro

IPR003089. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]

Pfam

PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PRINTS

PR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.

ProtoNet

Search...

Entry information

Entry name

PRXC_STRLI

Accession

Primary (citable) accession number: P49323

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)