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Protein

Nuclear autoantigenic sperm protein

Gene

NASP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity).By similarity

GO - Molecular functioni

  • Hsp90 protein binding Source: UniProtKB
  • protein complex binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication, Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear autoantigenic sperm protein
Short name:
NASP
Gene namesi
Name:NASP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:7644. NASP.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear chromatin Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000132780.
PharmGKBiPA31448.

Polymorphism and mutation databases

BioMutaiNASP.
DMDMi23503077.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001062992 – 788Nuclear autoantigenic sperm proteinAdd BLAST787

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei33N6-acetyllysineCombined sources1
Modified residuei123PhosphothreonineCombined sources1
Modified residuei127PhosphoserineCombined sources1
Modified residuei170PhosphothreonineCombined sources1
Modified residuei176PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Modified residuei191PhosphoserineCombined sources1
Modified residuei243N6-acetyllysineBy similarity1
Modified residuei244PhosphoserineCombined sources1
Modified residuei288N6-acetyllysineBy similarity1
Modified residuei299PhosphoserineCombined sources1
Modified residuei321PhosphoserineCombined sources1
Modified residuei390PhosphothreonineCombined sources1
Modified residuei397PhosphoserineCombined sources1
Modified residuei408PhosphoserineCombined sources1
Modified residuei421PhosphoserineCombined sources1
Modified residuei451PhosphoserineCombined sources1
Modified residuei464PhosphothreonineCombined sources1
Modified residuei477PhosphothreonineCombined sources1
Modified residuei480PhosphoserineCombined sources1
Modified residuei490PhosphothreonineCombined sources1
Modified residuei497PhosphoserineCombined sources1
Modified residuei503PhosphoserineCombined sources1
Modified residuei662PhosphoserineCombined sources1
Modified residuei683PhosphothreonineCombined sources1
Modified residuei705PhosphoserineCombined sources1
Modified residuei706PhosphoserineCombined sources1
Modified residuei726PhosphoserineCombined sources1
Cross-linki736Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Modified residuei745PhosphoserineCombined sources1
Modified residuei751PhosphoserineCombined sources1
Modified residuei756PhosphoserineCombined sources1
Isoform 2 (identifier: P49321-2)
Modified residuei138PhosphothreonineCombined sources1
Modified residuei141PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP49321.
MaxQBiP49321.
PaxDbiP49321.
PeptideAtlasiP49321.
PRIDEiP49321.

PTM databases

iPTMnetiP49321.
PhosphoSitePlusiP49321.
SwissPalmiP49321.

Miscellaneous databases

PMAP-CutDBP49321.

Expressioni

Tissue specificityi

Isoform 1 is testis- and sperm-specific.

Gene expression databases

BgeeiENSG00000132780.
CleanExiHS_NASP.
ExpressionAtlasiP49321. baseline and differential.
GenevisibleiP49321. HS.

Organism-specific databases

HPAiHPA028136.
HPA030518.
HPA030520.

Interactioni

Subunit structurei

Binds to linker H1 histones but not to core histones. Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
H3F3BP842433EBI-7038920,EBI-120658
H3F3CQ6NXT25EBI-7038920,EBI-2868501
HIST1H3DP684318EBI-7038920,EBI-79722
TERF2IPQ9NYB02EBI-716205,EBI-750109

GO - Molecular functioni

  • Hsp90 protein binding Source: UniProtKB
  • protein complex binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110759. 53 interactors.
DIPiDIP-47269N.
IntActiP49321. 29 interactors.
MINTiMINT-4656497.
STRINGi9606.ENSP00000255120.

Structurei

3D structure databases

ProteinModelPortaliP49321.
SMRiP49321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 76TPR 1Add BLAST34
Repeati542 – 575TPR 2Add BLAST34
Repeati584 – 617TPR 3Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 127Histone-bindingAdd BLAST12
Regioni211 – 244Histone-bindingAdd BLAST34
Regioni469 – 512Histone-bindingAdd BLAST44

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili136 – 164Sequence analysisAdd BLAST29
Coiled coili460 – 487Sequence analysisAdd BLAST28
Coiled coili597 – 665Sequence analysisAdd BLAST69
Coiled coili753 – 778Sequence analysisAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi716 – 722Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi111 – 658Glu-rich (acidic)Add BLAST548

Sequence similaritiesi

Belongs to the NASP family.Curated
Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG4563. Eukaryota.
ENOG4110P5E. LUCA.
GeneTreeiENSGT00390000016650.
HOGENOMiHOG000013120.
HOVERGENiHBG002186.
InParanoidiP49321.
KOiK11291.
OMAiSTEYEKE.
OrthoDBiEOG091G0PN0.
PhylomeDBiP49321.
TreeFamiTF317297.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR019544. Tetratricopeptide_SHNi-TPR_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF10516. SHNi-TPR. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P49321-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMESTATAA VAAELVSADK IEDVPAPSTS ADKVESLDVD SEAKKLLGLG
60 70 80 90 100
QKHLVMGDIP AAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR
110 120 130 140 150
MENGVLGNAL EGVHVEEEEG EKTEDESLVE NNDNIDEEAR EELREQVYDA
160 170 180 190 200
MGEKEEAKKT EDKSLAKPET DKEQDSEMEK GGREDMDISK SAEEPQEKVD
210 220 230 240 250
LTLDWLTETS EEAKGGAAPE GPNEAEVTSG KPEQEVPDAE EEKSVSGTDV
260 270 280 290 300
QEECREKGGQ EKQGEVIVSI EEKPKEVSEE QPVVTLEKQG TAVEVEAESL
310 320 330 340 350
DPTVKPVDVG GDEPEEKVVT SENEAGKAVL EQLVGQEVPP AEESPEVTTE
360 370 380 390 400
AAEASAVEAG SEVSEKPGQE APVLPKDGAV NGPSVVGDQT PIEPQTSIER
410 420 430 440 450
LTETKDGSGL EEKVRAKLVP SQEETKLSVE ESEAAGDGVD TKVAQGATEK
460 470 480 490 500
SPEDKVQIAA NEETQEREEQ MKEGEETEGS EEDDKENDKT EEMPNDSVLE
510 520 530 540 550
NKSLQENEEE EIGNLELAWD MLDLAKIIFK RQETKEAQLY AAQAHLKLGE
560 570 580 590 600
VSVESENYVQ AVEEFQSCLN LQEQYLEAHD RLLAETHYQL GLAYGYNSQY
610 620 630 640 650
DEAVAQFSKS IEVIENRMAV LNEQVKEAEG SSAEYKKEIE ELKELLPEIR
660 670 680 690 700
EKIEDAKESQ RSGNVAELAL KATLVESSTS GFTPGGGGSS VSMIASRKPT
710 720 730 740 750
DGASSSNCVT DISHLVRKKR KPEEESPRKD DAKKAKQEPE VNGGSGDAVP
760 770 780
SGNEVSENME EEAENQAESR AAVEGTVEAG ATVESTAC
Length:788
Mass (Da):85,238
Last modified:September 19, 2002 - v2
Checksum:i0F4EB2BBE71534E8
GO
Isoform 2 (identifier: P49321-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     138-476: Missing.

Show »
Length:449
Mass (Da):48,804
Checksum:iA69B7FE13AAA3433
GO
Isoform 3 (identifier: P49321-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MAMESTATAAVAAELVSADKIEDVPAPSTSADKVES → MFLLLLHLQIKWRATINLLSVTEDGLHFVEYYLNRIIH

Show »
Length:790
Mass (Da):86,268
Checksum:i6C7299FFBC62B4B2
GO
Isoform 4 (identifier: P49321-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-99: Missing.

Note: No experimental confirmation available.
Show »
Length:724
Mass (Da):78,450
Checksum:iB1917C4EF7BAE070
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14 – 15EL → DV in AAA36027 (PubMed:1426632).Curated2
Sequence conflicti159K → T in BAD96536 (Ref. 3) Curated1
Sequence conflicti183R → G in BAG61005 (PubMed:14702039).Curated1
Sequence conflicti348T → Q in AAA36027 (PubMed:1426632).Curated1
Sequence conflicti633Missing in AAA36027 (PubMed:1426632).Curated1
Sequence conflicti767 – 770AESR → LKRG in AAA36027 (PubMed:1426632).Curated4
Sequence conflicti777V → L in AAA36027 (PubMed:1426632).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052619620V → G.Corresponds to variant rs34618000dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0366161 – 36MAMES…DKVES → MFLLLLHLQIKWRATINLLS VTEDGLHFVEYYLNRIIH in isoform 3. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_04702836 – 99Missing in isoform 4. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_006551138 – 476Missing in isoform 2. 1 PublicationAdd BLAST339

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97856 mRNA. Translation: AAA36027.1.
AK056161 mRNA. No translation available.
AK291637 mRNA. Translation: BAF84326.1.
AK298893 mRNA. Translation: BAG61005.1.
AK222816 mRNA. Translation: BAD96536.1.
AL355480 Genomic DNA. Translation: CAI22462.1.
AL355480 Genomic DNA. Translation: CAI22465.1.
CH471059 Genomic DNA. Translation: EAX06968.1.
CH471059 Genomic DNA. Translation: EAX06969.1.
BC003113 mRNA. Translation: AAH03113.1.
BC053608 mRNA. Translation: AAH53608.1.
BC010105 mRNA. Translation: AAH10105.1.
BC011580 mRNA. Translation: AAH11580.1.
CCDSiCCDS524.1. [P49321-1]
CCDS525.1. [P49321-2]
CCDS55597.1. [P49321-4]
PIRiA48819.
RefSeqiNP_001182122.1. NM_001195193.1. [P49321-4]
NP_002473.2. NM_002482.3. [P49321-1]
NP_689511.2. NM_152298.3. [P49321-2]
XP_005270945.1. XM_005270888.2. [P49321-3]
UniGeneiHs.319334.

Genome annotation databases

EnsembliENST00000350030; ENSP00000255120; ENSG00000132780. [P49321-1]
ENST00000351223; ENSP00000255121; ENSG00000132780. [P49321-2]
ENST00000537798; ENSP00000438871; ENSG00000132780. [P49321-4]
GeneIDi4678.
KEGGihsa:4678.
UCSCiuc001coi.3. human. [P49321-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97856 mRNA. Translation: AAA36027.1.
AK056161 mRNA. No translation available.
AK291637 mRNA. Translation: BAF84326.1.
AK298893 mRNA. Translation: BAG61005.1.
AK222816 mRNA. Translation: BAD96536.1.
AL355480 Genomic DNA. Translation: CAI22462.1.
AL355480 Genomic DNA. Translation: CAI22465.1.
CH471059 Genomic DNA. Translation: EAX06968.1.
CH471059 Genomic DNA. Translation: EAX06969.1.
BC003113 mRNA. Translation: AAH03113.1.
BC053608 mRNA. Translation: AAH53608.1.
BC010105 mRNA. Translation: AAH10105.1.
BC011580 mRNA. Translation: AAH11580.1.
CCDSiCCDS524.1. [P49321-1]
CCDS525.1. [P49321-2]
CCDS55597.1. [P49321-4]
PIRiA48819.
RefSeqiNP_001182122.1. NM_001195193.1. [P49321-4]
NP_002473.2. NM_002482.3. [P49321-1]
NP_689511.2. NM_152298.3. [P49321-2]
XP_005270945.1. XM_005270888.2. [P49321-3]
UniGeneiHs.319334.

3D structure databases

ProteinModelPortaliP49321.
SMRiP49321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110759. 53 interactors.
DIPiDIP-47269N.
IntActiP49321. 29 interactors.
MINTiMINT-4656497.
STRINGi9606.ENSP00000255120.

PTM databases

iPTMnetiP49321.
PhosphoSitePlusiP49321.
SwissPalmiP49321.

Polymorphism and mutation databases

BioMutaiNASP.
DMDMi23503077.

Proteomic databases

EPDiP49321.
MaxQBiP49321.
PaxDbiP49321.
PeptideAtlasiP49321.
PRIDEiP49321.

Protocols and materials databases

DNASUi4678.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000350030; ENSP00000255120; ENSG00000132780. [P49321-1]
ENST00000351223; ENSP00000255121; ENSG00000132780. [P49321-2]
ENST00000537798; ENSP00000438871; ENSG00000132780. [P49321-4]
GeneIDi4678.
KEGGihsa:4678.
UCSCiuc001coi.3. human. [P49321-1]

Organism-specific databases

CTDi4678.
GeneCardsiNASP.
HGNCiHGNC:7644. NASP.
HPAiHPA028136.
HPA030518.
HPA030520.
MIMi603185. gene.
neXtProtiNX_P49321.
OpenTargetsiENSG00000132780.
PharmGKBiPA31448.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4563. Eukaryota.
ENOG4110P5E. LUCA.
GeneTreeiENSGT00390000016650.
HOGENOMiHOG000013120.
HOVERGENiHBG002186.
InParanoidiP49321.
KOiK11291.
OMAiSTEYEKE.
OrthoDBiEOG091G0PN0.
PhylomeDBiP49321.
TreeFamiTF317297.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132780-MONOMER.

Miscellaneous databases

ChiTaRSiNASP. human.
GeneWikiiNASP_(gene).
GenomeRNAii4678.
PMAP-CutDBP49321.
PROiP49321.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132780.
CleanExiHS_NASP.
ExpressionAtlasiP49321. baseline and differential.
GenevisibleiP49321. HS.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR019544. Tetratricopeptide_SHNi-TPR_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF10516. SHNi-TPR. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNASP_HUMAN
AccessioniPrimary (citable) accession number: P49321
Secondary accession number(s): A8K6H2
, B4DQP3, D3DQ07, F5H3J2, Q53GW5, Q5T622, Q5T625, Q96A69, Q9BTW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 19, 2002
Last modified: November 30, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.