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Protein

Heterogeneous nuclear ribonucleoprotein A1

Gene

Hnrnpa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection.

GO - Molecular functioni

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: MGI
  • mRNA transport Source: UniProtKB-KW
  • nuclear export Source: HGNC
  • nuclear import Source: HGNC
  • RNA splicing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein A1
Short name:
hnRNP A1
Alternative name(s):
HDP-1
Helix-destabilizing protein
Single-strand-binding protein
Topoisomerase-inhibitor suppressed
hnRNP core protein A1
Cleaved into the following chain:
Gene namesi
Name:Hnrnpa1
Synonyms:Fli-2, Hnrpa1, Tis
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:104820. Hnrnpa1.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs (By similarity). Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818301 – 320Heterogeneous nuclear ribonucleoprotein A1Add BLAST320
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004245112 – 320Heterogeneous nuclear ribonucleoprotein A1, N-terminally processedAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylserine; in Heterogeneous nuclear ribonucleoprotein A1, N-terminally processedBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei6PhosphoserineCombined sources1
Cross-linki8Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei22PhosphoserineCombined sources1
Cross-linki113Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei192Phosphoserine; by MKNK2By similarity1
Modified residuei194Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei194Dimethylated arginine; alternateBy similarity1
Modified residuei194Omega-N-methylarginine; alternateCombined sources1
Modified residuei199PhosphoserineBy similarity1
Modified residuei206Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei206Dimethylated arginine; alternateBy similarity1
Modified residuei206Omega-N-methylarginine; alternateCombined sources1
Modified residuei218Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei218Omega-N-methylarginine; alternateCombined sources1
Modified residuei225Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei225Dimethylated arginine; alternateBy similarity1
Modified residuei225Omega-N-methylarginine; alternateCombined sources1
Modified residuei232Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei232Omega-N-methylarginine; alternateCombined sources1
Modified residuei284Omega-N-methylarginineCombined sources1
Modified residuei285PhosphoserineBy similarity1
Modified residuei298N6-acetyllysineCombined sources1
Modified residuei300Omega-N-methylarginineCombined sources1
Modified residuei309PhosphoserineBy similarity1
Modified residuei310Phosphoserine; by MKNK2By similarity1
Modified residuei311Phosphoserine; by MKNK2By similarity1
Modified residuei312Phosphoserine; by MKNK2By similarity1
Modified residuei313PhosphoserineBy similarity1
Modified residuei316PhosphoserineBy similarity1
Modified residuei318Omega-N-methylarginineBy similarity1

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP49312.
MaxQBiP49312.
PaxDbiP49312.
PeptideAtlasiP49312.
PRIDEiP49312.
TopDownProteomicsiP49312-1. [P49312-1]

PTM databases

iPTMnetiP49312.
PhosphoSitePlusiP49312.

Expressioni

Gene expression databases

BgeeiENSMUSG00000046434.
CleanExiMM_HNRNPA1.
ExpressionAtlasiP49312. baseline and differential.
GenevisibleiP49312. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with SEPT6, C9orf72, KHDRBS1, UBQLN2.By similarity

Protein-protein interaction databases

BioGridi200357. 6 interactors.
IntActiP49312. 7 interactors.
MINTiMINT-1744029.
STRINGi10090.ENSMUSP00000137406.

Structurei

3D structure databases

ProteinModelPortaliP49312.
SMRiP49312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 97RRM 1PROSITE-ProRule annotationAdd BLAST84
Domaini105 – 184RRM 2PROSITE-ProRule annotationAdd BLAST80

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 94Globular A domainAdd BLAST91
Regioni95 – 185Globular B domainAdd BLAST91
Regioni218 – 240RNA-binding RGG-boxAdd BLAST23
Regioni268 – 305Nuclear targeting sequenceBy similarityAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi195 – 320Gly-richAdd BLAST126
Compositional biasi308 – 313Poly-Ser6

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00760000118873.
HOGENOMiHOG000234442.
HOVERGENiHBG002295.
InParanoidiP49312.
KOiK12741.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR021662. HnRNPA1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF11627. HnRNPA1. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P49312-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKSESPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN
60 70 80 90 100
TKRSRGFGFV TYATVEEVDA AMNARPHKVD GRVVEPKRAV SREDSQRPGA
110 120 130 140 150
HLTVKKIFVG GIKEDTEEHH LRDYFEQYGK IEVIEIMTDR GSGKKRGFAF
160 170 180 190 200
VTFDDHDSVD KIVIQKYHTV NGHNCEVRKA LSKQEMASAS SSQRGRSGSG
210 220 230 240 250
NFGGGRGGGF GGNDNFGRGG NFSGRGGFGG SRGGGGYGGS GDGYNGFGND
260 270 280 290 300
GSNFGGGGSY NDFGNYNNQS SNFGPMKGGN FGGRSSGPYG GGGQYFAKPR
310 320
NQGGYGGSSS SSSYGSGRRF
Length:320
Mass (Da):34,196
Last modified:January 23, 2007 - v2
Checksum:i59485C9FA1FF8AE1
GO
Isoform Short (identifier: P49312-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     252-303: Missing.

Show »
Length:268
Mass (Da):28,898
Checksum:i13A50CDCA6DFB3A2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005825252 – 303Missing in isoform Short. CuratedAdd BLAST52

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99167 mRNA. Translation: AAA37633.1.
D86729 mRNA. Translation: BAA13162.1.
D86728 mRNA. Translation: BAA13161.1.
AK007802 mRNA. Translation: BAB25267.1.
AK088308 mRNA. Translation: BAC40273.1.
AK131999 mRNA. Translation: BAE20929.1.
AK135391 mRNA. Translation: BAE22518.1.
AK161730 mRNA. Translation: BAE36552.1.
AK166901 mRNA. Translation: BAE39104.1.
AK167161 mRNA. Translation: BAE39302.1.
AK167810 mRNA. Translation: BAE39837.1.
AK167913 mRNA. Translation: BAE39920.1.
AK168420 mRNA. Translation: BAE40333.1.
AK168531 mRNA. Translation: BAE40409.1.
BC080675 mRNA. Translation: AAH80675.1.
BC083136 mRNA. Translation: AAH83136.1.
CCDSiCCDS37233.1. [P49312-1]
PIRiA44485.
RefSeqiNP_034577.1. NM_010447.5. [P49312-1]
UniGeneiMm.237064.
Mm.432031.

Genome annotation databases

EnsembliENSMUST00000087351; ENSMUSP00000084609; ENSMUSG00000046434. [P49312-1]
GeneIDi15382.
KEGGimmu:15382.
UCSCiuc007xxp.2. mouse. [P49312-1]
uc011zrp.1. mouse. [P49312-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99167 mRNA. Translation: AAA37633.1.
D86729 mRNA. Translation: BAA13162.1.
D86728 mRNA. Translation: BAA13161.1.
AK007802 mRNA. Translation: BAB25267.1.
AK088308 mRNA. Translation: BAC40273.1.
AK131999 mRNA. Translation: BAE20929.1.
AK135391 mRNA. Translation: BAE22518.1.
AK161730 mRNA. Translation: BAE36552.1.
AK166901 mRNA. Translation: BAE39104.1.
AK167161 mRNA. Translation: BAE39302.1.
AK167810 mRNA. Translation: BAE39837.1.
AK167913 mRNA. Translation: BAE39920.1.
AK168420 mRNA. Translation: BAE40333.1.
AK168531 mRNA. Translation: BAE40409.1.
BC080675 mRNA. Translation: AAH80675.1.
BC083136 mRNA. Translation: AAH83136.1.
CCDSiCCDS37233.1. [P49312-1]
PIRiA44485.
RefSeqiNP_034577.1. NM_010447.5. [P49312-1]
UniGeneiMm.237064.
Mm.432031.

3D structure databases

ProteinModelPortaliP49312.
SMRiP49312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200357. 6 interactors.
IntActiP49312. 7 interactors.
MINTiMINT-1744029.
STRINGi10090.ENSMUSP00000137406.

PTM databases

iPTMnetiP49312.
PhosphoSitePlusiP49312.

Proteomic databases

EPDiP49312.
MaxQBiP49312.
PaxDbiP49312.
PeptideAtlasiP49312.
PRIDEiP49312.
TopDownProteomicsiP49312-1. [P49312-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087351; ENSMUSP00000084609; ENSMUSG00000046434. [P49312-1]
GeneIDi15382.
KEGGimmu:15382.
UCSCiuc007xxp.2. mouse. [P49312-1]
uc011zrp.1. mouse. [P49312-2]

Organism-specific databases

CTDi3178.
MGIiMGI:104820. Hnrnpa1.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00760000118873.
HOGENOMiHOG000234442.
HOVERGENiHBG002295.
InParanoidiP49312.
KOiK12741.

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

ChiTaRSiHnrnpa1. mouse.
PROiP49312.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046434.
CleanExiMM_HNRNPA1.
ExpressionAtlasiP49312. baseline and differential.
GenevisibleiP49312. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR021662. HnRNPA1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF11627. HnRNPA1. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiROA1_MOUSE
AccessioniPrimary (citable) accession number: P49312
Secondary accession number(s): P97312, Q3V269
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.