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P49300 (CLC10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-type lectin domain family 10 member A
Alternative name(s):
MMGL
Macrophage asialoglycoprotein-binding protein 1
Short name=M-ASGP-BP-1
Macrophage galactose/N-acetylgalactosamine-specific lectin
Gene names
Name:Clec10a
Synonyms:Mgl, Mgl1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes terminal galactose and N-acetylgalactosamine units. May participate in the interaction between tumoricidal macrophages and tumor cells. Plays a role in the recruitment of inflammatory monocytes to adipose tissue in diet-induced obesity. Ref.9

Subunit structure

Homooligomer. Interacts with SIGLEC1, which may act as a counter-receptor for CLEC10A in lymph node. Ref.8

Subcellular location

Membrane; Single-pass type II membrane protein.

Tissue specificity

Detected in lymph node in the subcapsular sinus, interfollicular regions, T and B-cell boundary and in the areas surrounding high endothelial venules (at protein level). Expressed on the surface of activated macrophages. Expressed in heart, lung, testis, skeletal muscle, spleen, brain, kidney and thymus. Expressed in P388, RAW 264.7 and M1 cell lines. Ref.7 Ref.8

Developmental stage

Detected in E7, E11, E15 and E17 embryo. Ref.7

Sequence similarities

Contains 1 C-type lectin domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304C-type lectin domain family 10 member A
PRO_0000046657

Regions

Topological domain1 – 3535Cytoplasmic Potential
Transmembrane36 – 5621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain57 – 304248Extracellular Potential
Domain172 – 298127C-type lectin

Amino acid modifications

Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Disulfide bond173 ↔ 184 By similarity
Disulfide bond201 ↔ 296 By similarity
Disulfide bond274 ↔ 288 By similarity

Experimental info

Sequence conflict711T → I in AAH14811. Ref.5
Sequence conflict1281V → L in AAH14811. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P49300 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 3F79CD12C34F5BCC

FASTA30434,596
        10         20         30         40         50         60 
MIYENLQNSR IEEKTQEPGK APSQSFLWRI LSWTHLLLFS LGLSLLLLVV VSVIGSQNSQ 

        70         80         90        100        110        120 
LRRDLGTLRA TLDNTTSKIK AEFQSLDSRA DSFEKGISSL KVDVEDHRQE LQAGRDLSQK 

       130        140        150        160        170        180 
VTSLESTVEK REQALKTDLS DLTDHVQQLR KDLKALTCQL ANLKNNGSEV ACCPLHWTEH 

       190        200        210        220        230        240 
EGSCYWFSES EKSWPEADKY CRLENSHLVV VNSLEEQNFL QNRLANVVSW IGLTDQNGPW 

       250        260        270        280        290        300 
RWVDGTDFEK GFKNWAPLQP DNWFGHGLGG GEDCAHITTG GPWNDDVCQR TFRWICEMKL 


AKES 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of cDNA encoding a galactose/N-acetylgalactosamine-specific lectin on mouse tumoricidal macrophages."
Sato M., Kawakamyi K., Osawa T., Toyoshima S.
J. Biochem. 111:331-336(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H/HeN.
[2]"Genomic structure and chromosomal location of the mouse macrophage C-type lectin gene."
Tsuiji M., Fujimori M., Seldin M.F., Taketo M.M., Irimura T.
Immunogenetics 50:67-70(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]The mouse genome sequencing consortium
Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[6]"Purification and characterization of a lectin-like molecule specific for galactose/N-acetyl-galactosamine from tumoricidal macrophages."
Oda S., Sato M., Toyoshima S., Osawa T.
J. Biochem. 104:600-605(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 102-120 AND 137-151.
Strain: C3H/HeN.
[7]"Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1."
Tsuiji M., Fujimori M., Ohashi Y., Higashi N., Onami T.M., Hedrick S.M., Irimura T.
J. Biol. Chem. 277:28892-28901(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]"Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type C-type lectin 1."
Kumamoto Y., Higashi N., Denda-Nagai K., Tsuiji M., Sato K., Crocker P.R., Irimura T.
J. Biol. Chem. 279:49274-49280(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SIGLEC1, TISSUE SPECIFICITY.
[9]"MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity."
Westcott D.J., Delproposto J.B., Geletka L.M., Wang T., Singer K., Saltiel A.R., Lumeng C.N.
J. Exp. Med. 206:3143-3156(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S36676 mRNA. Translation: AAB22171.1.
AF132744 Genomic DNA. Translation: AAD31028.1.
CH466596 Genomic DNA. Translation: EDL12536.1.
AL669869 Genomic DNA. Translation: CAI35217.1.
CR933731 Genomic DNA. Translation: CAM28085.1.
BC014811 mRNA. Translation: AAH14811.1.
PIRJX0209.
RefSeqNP_034926.1. NM_010796.3.
UniGeneMm.252405.

3D structure databases

ProteinModelPortalP49300.
SMRP49300. Positions 173-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP49300. 1 interaction.
MINTMINT-4122085.
STRING10090.ENSMUSP00000000327.

Proteomic databases

PaxDbP49300.
PRIDEP49300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102571; ENSMUSP00000099631; ENSMUSG00000000318.
GeneID17312.
KEGGmmu:17312.
UCSCuc007jtx.2. mouse.

Organism-specific databases

CTD10462.
MGIMGI:96975. Clec10a.

Phylogenomic databases

eggNOGNOG259792.
GeneTreeENSGT00750000117265.
HOGENOMHOG000034093.
HOVERGENHBG000270.
KOK06721.
PhylomeDBP49300.

Gene expression databases

ArrayExpressP49300.
BgeeP49300.
GenevestigatorP49300.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
PROSITEPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291868.
PROP49300.
SOURCESearch...

Entry information

Entry nameCLC10_MOUSE
AccessionPrimary (citable) accession number: P49300
Secondary accession number(s): Q549F6, Q91YT3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot