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P49300

- CLC10_MOUSE

UniProt

P49300 - CLC10_MOUSE

Protein

C-type lectin domain family 10 member A

Gene

Clec10a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Recognizes terminal galactose and N-acetylgalactosamine units. May participate in the interaction between tumoricidal macrophages and tumor cells. Plays a role in the recruitment of inflammatory monocytes to adipose tissue in diet-induced obesity.1 Publication

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro

    Keywords - Ligandi

    Calcium, Lectin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-type lectin domain family 10 member A
    Alternative name(s):
    MMGL
    Macrophage asialoglycoprotein-binding protein 1
    Short name:
    M-ASGP-BP-1
    Macrophage galactose/N-acetylgalactosamine-specific lectin
    Gene namesi
    Name:Clec10a
    Synonyms:Mgl, Mgl1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:96975. Clec10a.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304C-type lectin domain family 10 member APRO_0000046657Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi173 ↔ 184PROSITE-ProRule annotation
    Disulfide bondi201 ↔ 296PROSITE-ProRule annotation
    Disulfide bondi274 ↔ 288PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP49300.
    PRIDEiP49300.

    Expressioni

    Tissue specificityi

    Detected in lymph node in the subcapsular sinus, interfollicular regions, T and B-cell boundary and in the areas surrounding high endothelial venules (at protein level). Expressed on the surface of activated macrophages. Expressed in heart, lung, testis, skeletal muscle, spleen, brain, kidney and thymus. Expressed in P388, RAW 264.7 and M1 cell lines.2 Publications

    Developmental stagei

    Detected in E7, E11, E15 and E17 embryo.1 Publication

    Gene expression databases

    ArrayExpressiP49300.
    BgeeiP49300.
    GenevestigatoriP49300.

    Interactioni

    Subunit structurei

    Homooligomer. Interacts with SIGLEC1, which may act as a counter-receptor for CLEC10A in lymph node.1 Publication

    Protein-protein interaction databases

    IntActiP49300. 1 interaction.
    MINTiMINT-4122085.
    STRINGi10090.ENSMUSP00000000327.

    Structurei

    3D structure databases

    ProteinModelPortaliP49300.
    SMRiP49300. Positions 136-301.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3535CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini57 – 304248ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini172 – 298127C-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG259792.
    GeneTreeiENSGT00750000117265.
    HOGENOMiHOG000034093.
    HOVERGENiHBG000270.
    KOiK06721.
    PhylomeDBiP49300.

    Family and domain databases

    Gene3Di3.10.100.10. 1 hit.
    InterProiIPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR005640. Lectin_N.
    [Graphical view]
    PfamiPF00059. Lectin_C. 1 hit.
    PF03954. Lectin_N. 1 hit.
    [Graphical view]
    SMARTiSM00034. CLECT. 1 hit.
    [Graphical view]
    SUPFAMiSSF56436. SSF56436. 1 hit.
    PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49300-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIYENLQNSR IEEKTQEPGK APSQSFLWRI LSWTHLLLFS LGLSLLLLVV    50
    VSVIGSQNSQ LRRDLGTLRA TLDNTTSKIK AEFQSLDSRA DSFEKGISSL 100
    KVDVEDHRQE LQAGRDLSQK VTSLESTVEK REQALKTDLS DLTDHVQQLR 150
    KDLKALTCQL ANLKNNGSEV ACCPLHWTEH EGSCYWFSES EKSWPEADKY 200
    CRLENSHLVV VNSLEEQNFL QNRLANVVSW IGLTDQNGPW RWVDGTDFEK 250
    GFKNWAPLQP DNWFGHGLGG GEDCAHITTG GPWNDDVCQR TFRWICEMKL 300
    AKES 304
    Length:304
    Mass (Da):34,596
    Last modified:February 1, 1996 - v1
    Checksum:i3F79CD12C34F5BCC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711T → I in AAH14811. (PubMed:15489334)Curated
    Sequence conflicti128 – 1281V → L in AAH14811. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S36676 mRNA. Translation: AAB22171.1.
    AF132744 Genomic DNA. Translation: AAD31028.1.
    CH466596 Genomic DNA. Translation: EDL12536.1.
    AL669869 Genomic DNA. Translation: CAI35217.1.
    CR933731 Genomic DNA. Translation: CAM28085.1.
    BC014811 mRNA. Translation: AAH14811.1.
    CCDSiCCDS24936.1.
    PIRiJX0209.
    RefSeqiNP_034926.1. NM_010796.3.
    UniGeneiMm.252405.

    Genome annotation databases

    EnsembliENSMUST00000102571; ENSMUSP00000099631; ENSMUSG00000000318.
    GeneIDi17312.
    KEGGimmu:17312.
    UCSCiuc007jtx.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    MGL1

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S36676 mRNA. Translation: AAB22171.1 .
    AF132744 Genomic DNA. Translation: AAD31028.1 .
    CH466596 Genomic DNA. Translation: EDL12536.1 .
    AL669869 Genomic DNA. Translation: CAI35217.1 .
    CR933731 Genomic DNA. Translation: CAM28085.1 .
    BC014811 mRNA. Translation: AAH14811.1 .
    CCDSi CCDS24936.1.
    PIRi JX0209.
    RefSeqi NP_034926.1. NM_010796.3.
    UniGenei Mm.252405.

    3D structure databases

    ProteinModelPortali P49300.
    SMRi P49300. Positions 136-301.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P49300. 1 interaction.
    MINTi MINT-4122085.
    STRINGi 10090.ENSMUSP00000000327.

    Proteomic databases

    PaxDbi P49300.
    PRIDEi P49300.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102571 ; ENSMUSP00000099631 ; ENSMUSG00000000318 .
    GeneIDi 17312.
    KEGGi mmu:17312.
    UCSCi uc007jtx.2. mouse.

    Organism-specific databases

    CTDi 10462.
    MGIi MGI:96975. Clec10a.

    Phylogenomic databases

    eggNOGi NOG259792.
    GeneTreei ENSGT00750000117265.
    HOGENOMi HOG000034093.
    HOVERGENi HBG000270.
    KOi K06721.
    PhylomeDBi P49300.

    Miscellaneous databases

    NextBioi 291868.
    PROi P49300.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P49300.
    Bgeei P49300.
    Genevestigatori P49300.

    Family and domain databases

    Gene3Di 3.10.100.10. 1 hit.
    InterProi IPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR005640. Lectin_N.
    [Graphical view ]
    Pfami PF00059. Lectin_C. 1 hit.
    PF03954. Lectin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00034. CLECT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56436. SSF56436. 1 hit.
    PROSITEi PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of cDNA encoding a galactose/N-acetylgalactosamine-specific lectin on mouse tumoricidal macrophages."
      Sato M., Kawakamyi K., Osawa T., Toyoshima S.
      J. Biochem. 111:331-336(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H/HeN.
    2. "Genomic structure and chromosomal location of the mouse macrophage C-type lectin gene."
      Tsuiji M., Fujimori M., Seldin M.F., Taketo M.M., Irimura T.
      Immunogenetics 50:67-70(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. The mouse genome sequencing consortium
      Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    6. "Purification and characterization of a lectin-like molecule specific for galactose/N-acetyl-galactosamine from tumoricidal macrophages."
      Oda S., Sato M., Toyoshima S., Osawa T.
      J. Biochem. 104:600-605(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 102-120 AND 137-151.
      Strain: C3H/HeN.
    7. "Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1."
      Tsuiji M., Fujimori M., Ohashi Y., Higashi N., Onami T.M., Hedrick S.M., Irimura T.
      J. Biol. Chem. 277:28892-28901(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    8. "Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type C-type lectin 1."
      Kumamoto Y., Higashi N., Denda-Nagai K., Tsuiji M., Sato K., Crocker P.R., Irimura T.
      J. Biol. Chem. 279:49274-49280(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SIGLEC1, TISSUE SPECIFICITY.
    9. "MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity."
      Westcott D.J., Delproposto J.B., Geletka L.M., Wang T., Singer K., Saltiel A.R., Lumeng C.N.
      J. Exp. Med. 206:3143-3156(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCLC10_MOUSE
    AccessioniPrimary (citable) accession number: P49300
    Secondary accession number(s): Q549F6, Q91YT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3