P49300 (CLC10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-type lectin domain family 10 member A

Alternative name(s):
MMGL
Macrophage asialoglycoprotein-binding protein 1
Short name=M-ASGP-BP-1
Macrophage galactose/N-acetylgalactosamine-specific lectin

Gene names

Name:	Clec10a
Synonyms:	Mgl, Mgl1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes terminal galactose and N-acetylgalactosamine units. May participate in the interaction between tumoricidal macrophages and tumor cells. Plays a role in the recruitment of inflammatory monocytes to adipose tissue in diet-induced obesity. Ref.9
Subunit structure	Homooligomer. Interacts with SIGLEC1, which may act as a counter-receptor for CLEC10A in lymph node. Ref.8
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Detected in lymph node in the subcapsular sinus, interfollicular regions, T and B cell boundary and in the areas surrounding high endothelial venules (at protein level). Expressed on the surface of activated macrophages. Expressed in heart, lung, testis, skeletal muscle, spleen, brain, kidney and thymus. Expressed in P388, RAW 264.7 and M1 cell lines. Ref.7 Ref.8
Developmental stage	Detected in E7, E11, E15 and E17 embryo. Ref.7
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Lectin
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	C-type lectin domain family 10 member A		PRO_0000046657
Regions
	Topological domain	1 – 35	35	Cytoplasmic Potential
	Transmembrane	36 – 56	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	57 – 304	248	Extracellular Potential
	Domain	172 – 298	127	C-type lectin
Amino acid modifications
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Disulfide bond	173 ↔ 184		By similarity
	Disulfide bond	201 ↔ 296		By similarity
	Disulfide bond	274 ↔ 288		By similarity
Experimental info
	Sequence conflict	71	1	T → I in AAH14811. Ref.5
	Sequence conflict	128	1	V → L in AAH14811. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P49300 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 3F79CD12C34F5BCC	FASTA	304	34,596
	10 20 30 40 50 60 MIYENLQNSR IEEKTQEPGK APSQSFLWRI LSWTHLLLFS LGLSLLLLVV VSVIGSQNSQ 70 80 90 100 110 120 LRRDLGTLRA TLDNTTSKIK AEFQSLDSRA DSFEKGISSL KVDVEDHRQE LQAGRDLSQK 130 140 150 160 170 180 VTSLESTVEK REQALKTDLS DLTDHVQQLR KDLKALTCQL ANLKNNGSEV ACCPLHWTEH 190 200 210 220 230 240 EGSCYWFSES EKSWPEADKY CRLENSHLVV VNSLEEQNFL QNRLANVVSW IGLTDQNGPW 250 260 270 280 290 300 RWVDGTDFEK GFKNWAPLQP DNWFGHGLGG GEDCAHITTG GPWNDDVCQR TFRWICEMKL AKES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of cDNA encoding a galactose/N-acetylgalactosamine-specific lectin on mouse tumoricidal macrophages." Sato M., Kawakamyi K., Osawa T., Toyoshima S. J. Biochem. 111:331-336(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/HeN.
[2]	"Genomic structure and chromosomal location of the mouse macrophage C-type lectin gene." Tsuiji M., Fujimori M., Seldin M.F., Taketo M.M., Irimura T. Immunogenetics 50:67-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	The mouse genome sequencing consortium Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[6]	"Purification and characterization of a lectin-like molecule specific for galactose/N-acetyl-galactosamine from tumoricidal macrophages." Oda S., Sato M., Toyoshima S., Osawa T. J. Biochem. 104:600-605(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 102-120 AND 137-151. Strain: C3H/HeN.
[7]	"Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1." Tsuiji M., Fujimori M., Ohashi Y., Higashi N., Onami T.M., Hedrick S.M., Irimura T. J. Biol. Chem. 277:28892-28901(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]	"Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type C-type lectin 1." Kumamoto Y., Higashi N., Denda-Nagai K., Tsuiji M., Sato K., Crocker P.R., Irimura T. J. Biol. Chem. 279:49274-49280(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SIGLEC1, TISSUE SPECIFICITY.
[9]	"MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity." Westcott D.J., Delproposto J.B., Geletka L.M., Wang T., Singer K., Saltiel A.R., Lumeng C.N. J. Exp. Med. 206:3143-3156(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

MGL1

Cross-references

Sequence databases
EMBL GenBank DDBJ	S36676 mRNA. Translation: AAB22171.1. AF132744 Genomic DNA. Translation: AAD31028.1. CH466596 Genomic DNA. Translation: EDL12536.1. AL669869 Genomic DNA. Translation: CAI35217.1. CR933731 Genomic DNA. Translation: CAM28085.1. BC014811 mRNA. Translation: AAH14811.1.
IPI	IPI00876198.
PIR	JX0209.
RefSeq	NP_034926.1. NM_010796.3.
UniGene	Mm.252405.
3D structure databases
ProteinModelPortal	P49300.
SMR	P49300. Positions 136-301.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000000327.
Proteomic databases
PaxDb	P49300.
PRIDE	P49300.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102571; ENSMUSP00000099631; ENSMUSG00000000318.
GeneID	17312.
KEGG	mmu:17312.
Organism-specific databases
CTD	10462.
MGI	MGI:96975. Clec10a.
Phylogenomic databases
eggNOG	NOG259792.
GeneTree	ENSGT00690000101986.
HOGENOM	HOG000034093.
HOVERGEN	HBG000270.
KO	K06721.
OrthoDB	EOG4V171K.
Gene expression databases
ArrayExpress	P49300.
Bgee	P49300.
Genevestigator	P49300.
GermOnline	ENSMUSG00000000318. Mus musculus.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. IPR005640. Lectin_N. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. PF03954. Lectin_N. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291868.
SOURCE	Search...

Entry information

Entry name

CLC10_MOUSE

Accession

Primary (citable) accession number: P49300
Secondary accession number(s): Q549F6, Q91YT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program