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Protein

C-type lectin domain family 10 member A

Gene

Clec10a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes terminal galactose and N-acetylgalactosamine units. May participate in the interaction between tumoricidal macrophages and tumor cells. Plays a role in the recruitment of inflammatory monocytes to adipose tissue in diet-induced obesity.1 Publication

GO - Molecular functioni

GO - Biological processi

  • connective tissue replacement involved in inflammatory response wound healing Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 10 member A
Alternative name(s):
MMGL
Macrophage asialoglycoprotein-binding protein 1
Short name:
M-ASGP-BP-1
Macrophage galactose/N-acetylgalactosamine-specific lectin
Gene namesi
Name:Clec10a
Synonyms:Mgl, Mgl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96975. Clec10a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei36 – 5621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini57 – 304248ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304C-type lectin domain family 10 member APRO_0000046657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi173 ↔ 184PROSITE-ProRule annotation
Disulfide bondi201 ↔ 296PROSITE-ProRule annotation
Disulfide bondi274 ↔ 288PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP49300.
PaxDbiP49300.
PRIDEiP49300.

Expressioni

Tissue specificityi

Detected in lymph node in the subcapsular sinus, interfollicular regions, T and B-cell boundary and in the areas surrounding high endothelial venules (at protein level). Expressed on the surface of activated macrophages. Expressed in heart, lung, testis, skeletal muscle, spleen, brain, kidney and thymus. Expressed in P388, RAW 264.7 and M1 cell lines.2 Publications

Developmental stagei

Detected in E7, E11, E15 and E17 embryo.1 Publication

Gene expression databases

BgeeiP49300.
ExpressionAtlasiP49300. baseline and differential.
GenevisibleiP49300. MM.

Interactioni

Subunit structurei

Homooligomer. Interacts with SIGLEC1, which may act as a counter-receptor for CLEC10A in lymph node.1 Publication

Protein-protein interaction databases

IntActiP49300. 1 interaction.
MINTiMINT-4122085.
STRINGi10090.ENSMUSP00000000327.

Structurei

3D structure databases

ProteinModelPortaliP49300.
SMRiP49300. Positions 136-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 298127C-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG259792.
GeneTreeiENSGT00760000118924.
HOGENOMiHOG000034093.
HOVERGENiHBG000270.
InParanoidiP49300.
PhylomeDBiP49300.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYENLQNSR IEEKTQEPGK APSQSFLWRI LSWTHLLLFS LGLSLLLLVV
60 70 80 90 100
VSVIGSQNSQ LRRDLGTLRA TLDNTTSKIK AEFQSLDSRA DSFEKGISSL
110 120 130 140 150
KVDVEDHRQE LQAGRDLSQK VTSLESTVEK REQALKTDLS DLTDHVQQLR
160 170 180 190 200
KDLKALTCQL ANLKNNGSEV ACCPLHWTEH EGSCYWFSES EKSWPEADKY
210 220 230 240 250
CRLENSHLVV VNSLEEQNFL QNRLANVVSW IGLTDQNGPW RWVDGTDFEK
260 270 280 290 300
GFKNWAPLQP DNWFGHGLGG GEDCAHITTG GPWNDDVCQR TFRWICEMKL

AKES
Length:304
Mass (Da):34,596
Last modified:February 1, 1996 - v1
Checksum:i3F79CD12C34F5BCC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711T → I in AAH14811 (PubMed:15489334).Curated
Sequence conflicti128 – 1281V → L in AAH14811 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36676 mRNA. Translation: AAB22171.1.
AF132744 Genomic DNA. Translation: AAD31028.1.
CH466596 Genomic DNA. Translation: EDL12536.1.
AL669869 Genomic DNA. Translation: CAI35217.1.
CR933731 Genomic DNA. Translation: CAM28085.1.
BC014811 mRNA. Translation: AAH14811.1.
CCDSiCCDS24936.1.
PIRiJX0209.
RefSeqiNP_034926.1. NM_010796.3.
UniGeneiMm.252405.

Genome annotation databases

EnsembliENSMUST00000102571; ENSMUSP00000099631; ENSMUSG00000000318.
GeneIDi17312.
UCSCiuc007jtx.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

MGL1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S36676 mRNA. Translation: AAB22171.1.
AF132744 Genomic DNA. Translation: AAD31028.1.
CH466596 Genomic DNA. Translation: EDL12536.1.
AL669869 Genomic DNA. Translation: CAI35217.1.
CR933731 Genomic DNA. Translation: CAM28085.1.
BC014811 mRNA. Translation: AAH14811.1.
CCDSiCCDS24936.1.
PIRiJX0209.
RefSeqiNP_034926.1. NM_010796.3.
UniGeneiMm.252405.

3D structure databases

ProteinModelPortaliP49300.
SMRiP49300. Positions 136-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49300. 1 interaction.
MINTiMINT-4122085.
STRINGi10090.ENSMUSP00000000327.

Proteomic databases

MaxQBiP49300.
PaxDbiP49300.
PRIDEiP49300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102571; ENSMUSP00000099631; ENSMUSG00000000318.
GeneIDi17312.
UCSCiuc007jtx.2. mouse.

Organism-specific databases

CTDi10462.
MGIiMGI:96975. Clec10a.

Phylogenomic databases

eggNOGiNOG259792.
GeneTreeiENSGT00760000118924.
HOGENOMiHOG000034093.
HOVERGENiHBG000270.
InParanoidiP49300.
PhylomeDBiP49300.

Miscellaneous databases

NextBioi291868.
PROiP49300.
SOURCEiSearch...

Gene expression databases

BgeeiP49300.
ExpressionAtlasiP49300. baseline and differential.
GenevisibleiP49300. MM.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of cDNA encoding a galactose/N-acetylgalactosamine-specific lectin on mouse tumoricidal macrophages."
    Sato M., Kawakamyi K., Osawa T., Toyoshima S.
    J. Biochem. 111:331-336(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/HeN.
  2. "Genomic structure and chromosomal location of the mouse macrophage C-type lectin gene."
    Tsuiji M., Fujimori M., Seldin M.F., Taketo M.M., Irimura T.
    Immunogenetics 50:67-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. The mouse genome sequencing consortium
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  6. "Purification and characterization of a lectin-like molecule specific for galactose/N-acetyl-galactosamine from tumoricidal macrophages."
    Oda S., Sato M., Toyoshima S., Osawa T.
    J. Biochem. 104:600-605(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 102-120 AND 137-151.
    Strain: C3H/HeN.
  7. "Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1."
    Tsuiji M., Fujimori M., Ohashi Y., Higashi N., Onami T.M., Hedrick S.M., Irimura T.
    J. Biol. Chem. 277:28892-28901(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type C-type lectin 1."
    Kumamoto Y., Higashi N., Denda-Nagai K., Tsuiji M., Sato K., Crocker P.R., Irimura T.
    J. Biol. Chem. 279:49274-49280(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SIGLEC1, TISSUE SPECIFICITY.
  9. "MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity."
    Westcott D.J., Delproposto J.B., Geletka L.M., Wang T., Singer K., Saltiel A.R., Lumeng C.N.
    J. Exp. Med. 206:3143-3156(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCLC10_MOUSE
AccessioniPrimary (citable) accession number: P49300
Secondary accession number(s): Q549F6, Q91YT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.