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P49299

- CYSZ_CUCMA

UniProt

P49299 - CYSZ_CUCMA

Protein

Citrate synthase, glyoxysomal

Gene
N/A
Organism
Cucurbita maxima (Pumpkin) (Winter squash)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei329 – 3291PROSITE-ProRule annotation
    Active sitei368 – 3681PROSITE-ProRule annotation
    Active sitei424 – 4241PROSITE-ProRule annotation

    GO - Molecular functioni

    1. transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase, glyoxysomal (EC:2.3.3.16)
    Alternative name(s):
    GCS
    OrganismiCucurbita maxima (Pumpkin) (Winter squash)
    Taxonomic identifieri3661 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4343Glyoxysome1 PublicationAdd
    BLAST
    Chaini44 – 516473Citrate synthase, glyoxysomalPRO_0000005490Add
    BLAST

    Proteomic databases

    PRIDEiP49299.

    Structurei

    3D structure databases

    ProteinModelPortaliP49299.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001369. Citrate_synth. 1 hit.
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49299-1 [UniParc]FASTAAdd to Basket

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    MPTDMELSPS NVARHRLAVL AAHLSAASLE PPVMASSLEA HCVSAQTMVA    50
    PPELVKGTLT IVDERTGKRY QVQVSEEGTI KATDLKKITT GPNDKGLKLY 100
    DPGYLNTAPV RSSISYIDGD LGILRYRGYP IEELAESSTY VEVAYLLMYG 150
    NLPSQSQLAD WEFAISQHSA VPQGLVDIIQ AMPHDAHPMG VLVSAMSALS 200
    VFHPDANPAL RGQDLYKSKQ VRDKQIARII GKAPTIAAAA YLRLAGRPPV 250
    LPSSNLSYSE NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST 300
    SAARHLASSG VDVFTALSGA VGALYGPLHG GANEAVLKML SEIGTVNNIP 350
    EFIEGVKNRK RKMSGFGHRV YKNYDPRAKV IRKLAEEVFS IVGRDPLIEV 400
    AVALEKAALS DEYFVKRKLY PNVDFYSGLI YRAMGFPPEF FTVLFAIPRM 450
    AGYLAHWRES LDDPDTKIIR PQQVYTGEWL RHYIPPNERL VPAKADRLGQ 500
    VSVSNASKRR LSGSGI 516
    Length:516
    Mass (Da):56,759
    Last modified:February 1, 1996 - v1
    Checksum:i162F6270284A3F6B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38132 mRNA. Translation: BAA07328.1.
    PIRiS53008.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38132 mRNA. Translation: BAA07328.1 .
    PIRi S53008.

    3D structure databases

    ProteinModelPortali P49299.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P49299.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00717 .

    Family and domain databases

    Gene3Di 1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view ]
    PANTHERi PTHR11739. PTHR11739. 1 hit.
    Pfami PF00285. Citrate_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001369. Citrate_synth. 1 hit.
    PRINTSi PR00143. CITRTSNTHASE.
    SUPFAMi SSF48256. SSF48256. 1 hit.
    PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a glyoxysomal citrate synthase that is synthesized as a precursor of higher molecular mass in pumpkin."
      Kato A., Hayashi M., Mori H., Nishimura M.
      Plant Mol. Biol. 27:377-390(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 44-51.
      Strain: cv. Kurokawa Amakuri Nankin.
      Tissue: Etiolated cotyledon.

    Entry informationi

    Entry nameiCYSZ_CUCMA
    AccessioniPrimary (citable) accession number: P49299
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3