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Protein

Citrate synthase, glyoxysomal

Gene
N/A
Organism
Cucurbita maxima (Pumpkin) (Winter squash)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei329 – 3291PROSITE-ProRule annotation
Active sitei368 – 3681PROSITE-ProRule annotation
Active sitei424 – 4241PROSITE-ProRule annotation

GO - Molecular functioni

  1. transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer Source: InterPro

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, glyoxysomal (EC:2.3.3.16)
Alternative name(s):
GCS
OrganismiCucurbita maxima (Pumpkin) (Winter squash)
Taxonomic identifieri3661 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343Glyoxysome1 PublicationAdd
BLAST
Chaini44 – 516473Citrate synthase, glyoxysomalPRO_0000005490Add
BLAST

Proteomic databases

PRIDEiP49299.

Structurei

3D structure databases

ProteinModelPortaliP49299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTDMELSPS NVARHRLAVL AAHLSAASLE PPVMASSLEA HCVSAQTMVA
60 70 80 90 100
PPELVKGTLT IVDERTGKRY QVQVSEEGTI KATDLKKITT GPNDKGLKLY
110 120 130 140 150
DPGYLNTAPV RSSISYIDGD LGILRYRGYP IEELAESSTY VEVAYLLMYG
160 170 180 190 200
NLPSQSQLAD WEFAISQHSA VPQGLVDIIQ AMPHDAHPMG VLVSAMSALS
210 220 230 240 250
VFHPDANPAL RGQDLYKSKQ VRDKQIARII GKAPTIAAAA YLRLAGRPPV
260 270 280 290 300
LPSSNLSYSE NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST
310 320 330 340 350
SAARHLASSG VDVFTALSGA VGALYGPLHG GANEAVLKML SEIGTVNNIP
360 370 380 390 400
EFIEGVKNRK RKMSGFGHRV YKNYDPRAKV IRKLAEEVFS IVGRDPLIEV
410 420 430 440 450
AVALEKAALS DEYFVKRKLY PNVDFYSGLI YRAMGFPPEF FTVLFAIPRM
460 470 480 490 500
AGYLAHWRES LDDPDTKIIR PQQVYTGEWL RHYIPPNERL VPAKADRLGQ
510
VSVSNASKRR LSGSGI
Length:516
Mass (Da):56,759
Last modified:February 1, 1996 - v1
Checksum:i162F6270284A3F6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38132 mRNA. Translation: BAA07328.1.
PIRiS53008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38132 mRNA. Translation: BAA07328.1.
PIRiS53008.

3D structure databases

ProteinModelPortaliP49299.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP49299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00717.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of a glyoxysomal citrate synthase that is synthesized as a precursor of higher molecular mass in pumpkin."
    Kato A., Hayashi M., Mori H., Nishimura M.
    Plant Mol. Biol. 27:377-390(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 44-51.
    Strain: cv. Kurokawa Amakuri Nankin.
    Tissue: Etiolated cotyledon.

Entry informationi

Entry nameiCYSZ_CUCMA
AccessioniPrimary (citable) accession number: P49299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 1, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.