ID   CISY_CITMA              Reviewed;         471 AA.
AC   P49298;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   13-SEP-2023, entry version 95.
DE   RecName: Full=Citrate synthase, mitochondrial;
DE            EC=2.3.3.16;
DE   Flags: Precursor;
GN   Name=CIT;
OS   Citrus maxima (Pomelo) (Citrus grandis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=37334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Siamese Sweet 2240;
RA   Canel C.;
RL   Thesis (1994), University of California Riverside, United States.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; U19481; AAA82743.1; -; mRNA.
DR   AlphaFoldDB; P49298; -.
DR   SMR; P49298; -.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   NCBIfam; TIGR01793; cit_synth_euk; 1.
DR   PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR11739:SF8; CITRATE SYNTHASE, MITOCHONDRIAL; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Mitochondrion; Transferase; Transit peptide; Tricarboxylic acid cycle.
FT   TRANSIT         1..18
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..471
FT                   /note="Citrate synthase, mitochondrial"
FT                   /id="PRO_0000005486"
FT   ACT_SITE        307
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        353
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        408
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   471 AA;  52183 MW;  8EE1E23F7E154806 CRC64;
     MASLRSATAL SRLRSRAGQQ SNLSNSVRWL QMQSSADLDL HSQLKEMIPE QQERLKKVKS
     DLGKAQLGNI TIDVVIGGMR GMTGLLWETS LLDPDEGIRF RGLSIPECQK LLPAAKPDGE
     PLPEGLLWLL LTGKVPSKEQ VDGLSKELRD RATVPDYVYK AIDALPVSAH PMTQFASGVM
     ALQVQSEFQE AYEKGIHKSK SWEPTSEDSL NLIARVPVVA AYVYQRIYKD GKIIPKDDSL
     DYGGNFSHML GFDDPKMLEL MRLYVTIHSD HEGGNVSAHT GHLVASALSD PYLSFLAALN
     GLAGPLHGLA NQEVLLWIKS VVDECGENVT TEQLKDYVWK TLNSGKVVPG FGHGVLRKTD
     PRYTCQREFA LKHLPDDPLF QLVSKLYEVV PPILTKLGKV KNPWPNVDAH SGVLLNHFGL
     AEARYYTVLF GVSRSLGICS QLIWDRALGL PLERPKSVTL DWIEKNCKKA A
//