P49297 (ACEA_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Isocitrate lyase Short name=ICL Short name=Isocitrase Short name=Isocitratase EC=4.1.3.1
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in storage lipid mobilization during the growth of higher plant seedling.
Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Isocitrate lyase		PRO_0000068808
Regions
	Motif	573 – 575	3	Microbody targeting signal Potential
Sites
	Active site	213	1	Probable

Sequences

	Sequence		Length	Mass (Da)
	P49297 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F398FA96C7110B4E	FASTA	575	64,720
	10 20 30 40 50 60 MAASYSVPSM IMEEERRFEA EVAEVQAWWN TERFRLTKRA YSARDVVALR GTMRQSYASN 70 80 90 100 110 120 ELAQKLWRTL KTHQANGTAS RTFGALDPVQ VTMMAKHLDT IYVSGWQCSS THTSTNEPGP 130 140 150 160 170 180 DLADYPYDTV PNKVEHLFFA QQYHDRKQRE ARMSMCREER ARTPFIDYLK PIIADGDTGF 190 200 210 220 230 240 GGATATVKLC KLFVERGAAG VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV 250 260 270 280 290 300 MGTETVLVAR TDAVAATLIQ TNVDTRDHQF ILGASNPNLK GKGLATHLSE AMAAGKTGPE 310 320 330 340 350 360 LQAIEDNWLG MAELKTFSQC VTDAIKKMNL AEYEKQRKLN EWMNHSSYEK CLSHEQAREV 370 380 390 400 410 420 AERLGLPNLF WDWDLPRTRE GFYRFQGSVE AAIVRGWAFA EYCDLVWMET SSPDMVECTK 430 440 450 460 470 480 FSQGVKTLRP ELMLAYNLSP SFNWDASGMN DNQMMDFIPR IAKLGYCWQF ITLAGFHADA 490 500 510 520 530 540 LIVDTFAKDF ARRGMLAYVE KIQREERSNG VDTLAHQKWS GANYYDRVLR TVQGGITSTA 550 560 570 AMGKGVTEEQ FEEKWTRTGA TNLGDGSVVI AKARM

References

[1]	"A cDNA clone for isocitrate lyase from tomato." Janssen B.-J. Plant Physiol. 108:1339-1339(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. UC82B. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18678 mRNA. Translation: AAA82738.1.
PIR	T06353.
RefSeq	NP_001233878.1. NM_001246949.1.
UniGene	Les.2767.
3D structure databases
ProteinModelPortal	P49297.
SMR	P49297. Positions 9-535.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	544276.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_SOLLC

Accession

Primary (citable) accession number: P49297

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program