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P49296 (ACEA_CUCSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate lyase

Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1
OrganismCucumis sativus (Cucumber)
Taxonomic identifier3659 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in storage lipid mobilization during the growth of higher plant seedling.

Catalytic activity

Isocitrate = succinate + glyoxylate.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Subunit structure

Homotetramer By similarity.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisocitrate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Isocitrate lyase
PRO_0000068804

Regions

Motif574 – 5763Microbody targeting signal Potential

Sites

Active site2131 Probable

Sequences

Sequence LengthMass (Da)Tools
P49296 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 717D30B83CD81D6C

FASTA57664,611
        10         20         30         40         50         60 
MAASFSVPSM IMEEEGRFEA EVAEVQAWWN SERFKLTRRP YTAKDVVSLR GSLRQSYASN 

        70         80         90        100        110        120 
DLAKKLWRTL KTHQANGTAS RTFGALDPVQ VTMMAKHLDT IYVSGWQCSS THTSTNEPGP 

       130        140        150        160        170        180 
DLADYPYDTV PNKVEHLFFA QQYHDRKQRE ARMSMSREER AKTPYIDYLK PIIADGDTGF 

       190        200        210        220        230        240 
GGTTATVKLC KLFVERGAAG VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV 

       250        260        270        280        290        300 
MGVETILVAR TDAVAATLIQ TNVDKRDHQF ILGATNPNLR GKSLAGALAE AMAAGKTGAE 

       310        320        330        340        350        360 
LQALEDQWIS MAQLKTFSEC VTDAIMNTNA TENEKRRKLD EWMNHSSYEK CISNEQGREI 

       370        380        390        400        410        420 
AEKLGLKNLF WDWDLPRTRE GFYRFKGSVM AAIVRGWAFA PHADLIWMET SSPDLVECTT 

       430        440        450        460        470        480 
FAKGMKSIHP ETMLAYNLSP SFNWDASGMS DKQMEEFIPR IARLGFCWQF ITLAGFHADA 

       490        500        510        520        530        540 
LVVDTFARDY ARRGMLAYVE RIQREERNNG VDTLAHQKWS GANYYDRYLK TVQGGISSTA 

       550        560        570 
AMGKGVTEEQ FKESWTREGA VNLGEEGNVV VAKSRM 

« Hide

References

[1]"The isocitrate lyase gene of cucumber: isolation, characterisation and expression in cotyledons following seed germination."
Reynolds S.J., Smith S.M.
Plant Mol. Biol. 27:487-497(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Masterpiece.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z35499 Genomic DNA. Translation: CAA84632.1.
PIRS53505.
RefSeqXP_004158557.1. XM_004158509.1.

3D structure databases

ProteinModelPortalP49296.
SMRP49296. Positions 9-535.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101214114.
KEGGcsv:101214114.

Phylogenomic databases

KOK01637.

Enzyme and pathway databases

UniPathwayUPA00703; UER00719.

Family and domain databases

Gene3D3.20.20.60. 2 hits.
InterProIPR006254. Isocitrate_lyase.
IPR000918. Isocitrate_lyase/Pmutase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamPF00463. ICL. 1 hit.
[Graphical view]
PIRSFPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMSSF51621. SSF51621. 1 hit.
TIGRFAMsTIGR01346. isocit_lyase. 1 hit.
PROSITEPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACEA_CUCSA
AccessionPrimary (citable) accession number: P49296
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways