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Protein

Isocitrate lyase

Gene
N/A
Organism
Cucumis sativus (Cucumber)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in storage lipid mobilization during the growth of higher plant seedling.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase
  2. Malate synthase, glyoxysomal, Malate synthase (Csa_1G050360)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi175MagnesiumBy similarity1
Active sitei213Proton acceptorBy similarity1
Binding sitei250SubstrateBy similarity1
Binding sitei472SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitratsysaseBy similarity
OrganismiCucumis sativus (Cucumber)
Taxonomic identifieri3659 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688041 – 576Isocitrate lyaseAdd BLAST576

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP49296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 106Substrate bindingBy similarity3
Regioni214 – 215Substrate bindingBy similarity2
Regioni437 – 441Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi574 – 576Microbody targeting signalSequence analysis3

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASFSVPSM IMEEEGRFEA EVAEVQAWWN SERFKLTRRP YTAKDVVSLR
60 70 80 90 100
GSLRQSYASN DLAKKLWRTL KTHQANGTAS RTFGALDPVQ VTMMAKHLDT
110 120 130 140 150
IYVSGWQCSS THTSTNEPGP DLADYPYDTV PNKVEHLFFA QQYHDRKQRE
160 170 180 190 200
ARMSMSREER AKTPYIDYLK PIIADGDTGF GGTTATVKLC KLFVERGAAG
210 220 230 240 250
VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV MGVETILVAR
260 270 280 290 300
TDAVAATLIQ TNVDKRDHQF ILGATNPNLR GKSLAGALAE AMAAGKTGAE
310 320 330 340 350
LQALEDQWIS MAQLKTFSEC VTDAIMNTNA TENEKRRKLD EWMNHSSYEK
360 370 380 390 400
CISNEQGREI AEKLGLKNLF WDWDLPRTRE GFYRFKGSVM AAIVRGWAFA
410 420 430 440 450
PHADLIWMET SSPDLVECTT FAKGMKSIHP ETMLAYNLSP SFNWDASGMS
460 470 480 490 500
DKQMEEFIPR IARLGFCWQF ITLAGFHADA LVVDTFARDY ARRGMLAYVE
510 520 530 540 550
RIQREERNNG VDTLAHQKWS GANYYDRYLK TVQGGISSTA AMGKGVTEEQ
560 570
FKESWTREGA VNLGEEGNVV VAKSRM
Length:576
Mass (Da):64,611
Last modified:February 1, 1996 - v1
Checksum:i717D30B83CD81D6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35499 Genomic DNA. Translation: CAA84632.1.
PIRiS53505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35499 Genomic DNA. Translation: CAA84632.1.
PIRiS53505.

3D structure databases

ProteinModelPortaliP49296.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACEA_CUCSA
AccessioniPrimary (citable) accession number: P49296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 5, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.