Reviewed,
UniProtKB/Swiss-Prot P49295 (HEM12_CUCSA)
Last modified
November 25, 2008.
Version 60.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glutamyl-tRNA reductase 2, chloroplastic Short name=GluTR EC=1.2.1.70 | ||
| Gene names |
| ||
| Organism | Cucumis sativus (Cucumber) | ||
| Taxonomic identifier | 3659 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis |
Protein attributes
| Sequence length | 542 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA) By similarity. |
| Catalytic activity | L-glutamate 1-semialdehyde + NADP(+) + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH. |
| Pathway | |
| Subcellular location | Plastid › chloroplastBy similarity. |
| Tissue specificity | Found in all tissues examined. |
| Miscellaneous | During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity. |
| Sequence similarities | Belongs to the glutamyl-tRNA reductase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Chlorophyll biosynthesis Porphyrin biosynthesis |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NADP binding Inferred from electronic annotation. Source: InterPro glutamyl-tRNA reductase activityInferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Chloroplast Potential | |||||||
| Chain | ? – 542 | Glutamyl-tRNA reductase 2, chloroplastic | PRO_0000013310 | ||||||
Regions | |||||||||
| Nucleotide binding | 284 – 289 | 6 | NADP By similarity | ||||||
| Region | 142 – 145 | 4 | Substrate binding By similarity | ||||||
| Region | 207 – 209 | 3 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 143 | 1 | Nucleophile By similarity | ||||||
| Binding site | 202 | 1 | Substrate By similarity | ||||||
| Binding site | 213 | 1 | Substrate By similarity | ||||||
| Site | 192 | 1 | Important for activity By similarity | ||||||
Sequences
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References
| [1] | "Differential expression of two hemA mRNAs encoding glutamyl-tRNA reductase proteins in greening cucumber seedlings." Tanaka R., Yoshida K., Nakayashiki T., Masuda T., Tsuji H., Inokuchi H., Tanaka A. Plant Physiol. 110:1223-1230(1996) [PubMed: 8934625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Aonagajibai. Tissue: Cotyledon. |
Cross-references
Sequence databases | |
|---|---|
| D67088 mRNA. Translation: BAA11091.1. | |
| PIR | T10245. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GPJ based on UniProtKB Q9UXR8. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR000343. 4pyrrol_synth_GluRdtase. IPR015896. 4pyrrol_synth_GluRdtase_C. IPR015895. 4pyrrol_synth_GluRdtase_N. IPR016040. NAD(P)-bd. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF00745. GlutR_dimer. 1 hit. PF05201. GlutR_N. 1 hit. PF01488. Shikimate_DH. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01035. hemA. 1 hit. |
| PROSITE | PS00747. GLUTR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HEM12_CUCSA | ||||||||
| Accession | Primary (citable) accession number: P49295 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


