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Protein

Glutamyl-tRNA reductase 2, chloroplastic

Gene

HEMA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). Probably involved in wound-induced supply of heme to defensive hemoproteins outside plastids.3 Publications

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).By similarity

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (AXX17_At1g09980), Glutamyl-tRNA reductase 2, chloroplastic (HEMA2), Glutamyl-tRNA reductase 1, chloroplastic (HEMA1), Glutamyl-tRNA reductase (AXX17_At1g52270), Glutamyl-tRNA reductase (AXX17_At2g27450), Probable glutamyl-tRNA reductase 3, chloroplastic (HEMA3)
  2. Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (GSA1), Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic (GSA2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei135NucleophileBy similarity1
Sitei184Important for activityBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei205SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi277 – 282NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

  • chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  • heme biosynthetic process Source: TAIR
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  • response to oxidative stress Source: TAIR
  • tetrapyrrole biosynthetic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Porphyrin biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT1G09940-MONOMER
MetaCyc:AT1G09940-MONOMER
UniPathwayiUPA00251; UER00316
UPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductase 2, chloroplastic (EC:1.2.1.70)
Short name:
GluTR
Gene namesi
Name:HEMA2
Ordered Locus Names:At1g09940
ORF Names:F21M12.33
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G09940
TAIRilocus:2024392 AT1G09940

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, but decreased heme content in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 64ChloroplastSequence analysisAdd BLAST64
ChainiPRO_000001330865 – 530Glutamyl-tRNA reductase 2, chloroplasticAdd BLAST466

Proteomic databases

PaxDbiP49294
PRIDEiP49294

Expressioni

Tissue specificityi

Expressed in roots and flowers. Detected in leaves, hypocotyls and cotyledons.4 Publications

Inductioni

Not regulated by light. Up-regulated localy by wounding and reactive oxygen species. Not regulated by methyl jasmonate.3 Publications

Gene expression databases

ExpressionAtlasiP49294 baseline and differential
GenevisibleiP49294 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G09940.1

Structurei

3D structure databases

ProteinModelPortaliP49294
SMRiP49294
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 137Substrate bindingBy similarity4
Regioni199 – 201Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0373 LUCA
HOGENOMiHOG000109651
InParanoidiP49294
KOiK02492
OMAiYTERIIA
OrthoDBiEOG093609MY
PhylomeDBiP49294

Family and domain databases

Gene3Di3.30.460.30, 1 hit
HAMAPiMF_00087 Glu_tRNA_reductase, 1 hit
InterProiView protein in InterPro
IPR000343 4pyrrol_synth_GluRdtase
IPR015896 4pyrrol_synth_GluRdtase_dimer
IPR015895 4pyrrol_synth_GluRdtase_N
IPR018214 GluRdtase_CS
IPR036453 GluRdtase_dimer_dom_sf
IPR036343 GluRdtase_N_sf
IPR036291 NAD(P)-bd_dom_sf
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF00745 GlutR_dimer, 1 hit
PF05201 GlutR_N, 1 hit
PF01488 Shikimate_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF69075 SSF69075, 1 hit
SSF69742 SSF69742, 1 hit
TIGRFAMsiTIGR01035 hemA, 1 hit
PROSITEiView protein in PROSITE
PS00747 GLUTR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSSAFVVT PKLEKLLANH HNPTYSSSPA PLDVIGIRAL PMNNRNKRGL
60 70 80 90 100
IQRARCEISP SNKAASISAL EQLKTSAIDR YTKERSSIVV IGLSIHTAPV
110 120 130 140 150
EMREKLAIPE AEWPRAIAEL CGLNHIEEAA VLSTCNRMEI YVLALSQHRG
160 170 180 190 200
VKEVTEWMSK TSGIPVSEIC QHRFLLYNKD VTQHIFEVSA GLDSLVLGEG
210 220 230 240 250
QILAQVKQVV KVGQGVNGFG RNISGLFKHA ITVGKRVRTE TNIAAGAVSV
260 270 280 290 300
SSAAVELALM KLPESSHASS ARMLVVGAGK MGKLVIKHLV AKGCTKMVVV
310 320 330 340 350
NRSEEKVAAV RNEMPPGVEI IYKPLDEMLS CAAEADVVFT STASETPLFL
360 370 380 390 400
KEQVETLPPV RDARLFVDIS VPRNVGSCVA EIDGTRVFNV DDLKEVVAAN
410 420 430 440 450
KEDRVRKAMD AQAIITDESK HFEAWRDSLE TVPTIKKLRG YTERIIAAEI
460 470 480 490 500
EKSLPKMGID MNKKMRKTVD DLIRGIVNKL LHGPMQHLRC DGNDSRTLSE
510 520 530
TLDNMQALNR MYGLDAEILE EKIRAKVEKK
Length:530
Mass (Da):58,292
Last modified:July 24, 2007 - v2
Checksum:i0C0224296BA3D0A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245A → G in BAF00558 (Ref. 5) Curated1
Sequence conflicti410D → E in AAB01674 (PubMed:8605295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27118 Genomic DNA Translation: AAB01674.1
AC000132 Genomic DNA Translation: AAB60749.1
CP002684 Genomic DNA Translation: AEE28519.1
BT023439 mRNA Translation: AAY56430.1
AK228651 mRNA Translation: BAF00558.1
PIRiG86233
S65773
RefSeqiNP_172465.1, NM_100868.3
UniGeneiAt.27711

Genome annotation databases

EnsemblPlantsiAT1G09940.1; AT1G09940.1; AT1G09940
GeneIDi837528
GrameneiAT1G09940.1; AT1G09940.1; AT1G09940
KEGGiath:AT1G09940

Similar proteinsi

Entry informationi

Entry nameiHEM12_ARATH
AccessioniPrimary (citable) accession number: P49294
Secondary accession number(s): O04950, Q0WQP1, Q4V3B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 24, 2007
Last modified: April 25, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health