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Protein

Mite allergen Der f 6

Gene

DERF6

Organism
Dermatophagoides farinae (American house dust mite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Specificity similar to chymotrypsin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941Charge relay systemBy similarity
Active sitei140 – 1401Charge relay systemBy similarity
Active sitei233 – 2331Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.245.

Names & Taxonomyi

Protein namesi
Recommended name:
Mite allergen Der f 6 (EC:3.4.21.-)
Alternative name(s):
Allergen Der f VI
DF5
Allergen: Der f 6
Gene namesi
Name:DERF6
OrganismiDermatophagoides farinae (American house dust mite)
Taxonomic identifieri6954 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAcariAcariformesSarcoptiformesAstigmataPsoroptidiaAnalgoideaPyroglyphidaeDermatophagoidinaeDermatophagoides

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei306. Der f 6.
3255. Der f 6.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Propeptidei20 – 49301 PublicationPRO_0000028147Add
BLAST
Chaini50 – 279230Mite allergen Der f 6PRO_0000028148Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi79 ↔ 95PROSITE-ProRule annotation
Disulfide bondi204 ↔ 220PROSITE-ProRule annotation
Disulfide bondi229 ↔ 255PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP49276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 279230Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKIFLVTIL IVITVTVDAR FPRSLQPKWA YLDSNEFPRS KIGDSPIAGV
60 70 80 90 100
VGGQDADLAE APFQISLLKD YLIMKRHMCG GSLISESTVV TAAHCTYGQK
110 120 130 140 150
ASSLSVRYGT NQRTSSSYGD LKVKPIIQHE SYEQDQTQTD KTIIILPNPV
160 170 180 190 200
VPSTNVQMNE IETEDIVDGD KVTIYGWGLT DGNGKDLPDK LQKGSMTIVG
210 220 230 240 250
NDRCNEKWGS INAIHPGMIC ALDKTQSGCN GDSGGPLVSA NRKLTGIVSW
260 270
GPSKCPPGEY MSVFTRPKYY LDWITKNIV
Length:279
Mass (Da):30,535
Last modified:June 20, 2001 - v2
Checksum:i5B29B6C46F185E56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501V → A AA sequence (PubMed:8334537).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125187 mRNA. Translation: AAF28423.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125187 mRNA. Translation: AAF28423.1.

3D structure databases

ProteinModelPortaliP49276.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei306. Der f 6.
3255. Der f 6.0101.
MEROPSiS01.245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of Der f 6, a serine protease allergen from the house dust mite, Dermatophagoides farinae."
    Kawamoto S., Mizuguchi Y., Morimoto K., Aki T., Shigeta S., Yasueda H., Wada T., Suzuki O., Jyo T., Ono K.
    Biochim. Biophys. Acta 1454:201-207(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Allergens from Dermatophagoides mites with chymotryptic activity."
    Yasueda H., Mita H., Akiyama K., Shida T., Ando T., Sugiyama S., Yamakawa H.
    Clin. Exp. Allergy 23:384-390(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 50-69.

Entry informationi

Entry nameiDERF6_DERFA
AccessioniPrimary (citable) accession number: P49276
Secondary accession number(s): Q9NJS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: June 20, 2001
Last modified: March 16, 2016
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.