P49275 (DERF3_DERFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mite allergen Der f 3
EC=3.4.21.-

Alternative name(s):
Allergen Der f III
Allergen=Der f 3

Gene names

Name:

DERF3

Organism

Dermatophagoides farinae (American house dust mite)

Taxonomic identifier

6954 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Acari › Acariformes › Sarcoptiformes › Astigmata › Psoroptidia › Analgoidea › Pyroglyphidae › Dermatophagoidinae › Dermatophagoides

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Allergenic properties	Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 27	11			PRO_0000028143
	Chain	28 – 259	232	Mite allergen Der f 3		PRO_0000028144
Regions
	Domain	28 – 258	231	Peptidase S1
Sites
	Active site	67	1	Charge relay system By similarity
	Active site	112	1	Charge relay system By similarity
	Active site	212	1	Charge relay system By similarity
	Site	206	1	Required for specificity
Amino acid modifications
	Disulfide bond	52 ↔ 68		By similarity
	Disulfide bond	179 ↔ 196		By similarity
	Disulfide bond	208 ↔ 234		By similarity
Experimental info
	Sequence conflict	35	1	Q → K in AAA99805. Ref.2
	Sequence conflict	35	1	Q → L AA sequence Ref.3
	Sequence conflict	42	1	Q → E AA sequence Ref.3
	Sequence conflict	46 – 47	2	QS → EV AA sequence Ref.3
	Sequence conflict	136	1	A → P in AAA99805. Ref.2
	Sequence conflict	219	1	V → I in AAA99805. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P49275 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 043A62B26C4C57D7	FASTA	259	27,674
	10 20 30 40 50 60 MMILTIVVLL AANILATPIL PSSPNATIVG GVKAQAGDCP YQISLQSSSH FCGGSILDEY 70 80 90 100 110 120 WILTAAHCVN GQSAKKLSIR YNTLKHASGG EKIQVAEIYQ HENYDSMTID NDVALIKLKT 130 140 150 160 170 180 PMTLDQTNAK PVPLPAQGSD VKVGDKIRVS GWGYLQEGSY SLPSELQRVD IDVVSREQCD 190 200 210 220 230 240 QLYSKAGADV SENMICGGDV ANGGVDSCQG DSGGPVVDVA TKQIVGIVSW GYGCARKGYP 250 GVYTRVGNFV DWIESKRSQ

References

[1]	"Cloning and expression in Escherichia coli of cDNA encoding house dust mite allergen Der f 3, serine protease from Dermatophagoides farinae." Nishiyama C., Yasuhara T., Yuuki T., Okumura Y. FEBS Lett. 377:62-66(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Comparative analysis of the genes encoding group 3 allergens from Dermatophagoides pteronyssinus and Dermatophagoides farinae." Smith W.A., Thomas W.R. Int. Arch. Allergy Immunol. 109:133-140(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-259.
[3]	"Antigenic and structural analysis of group II allergens (Der f II and Der p II) from house dust mites (Dermatophagoides spp)." Heymann P.W., Chapman M.D., Aalberse R.C., Fox J.W., Platts-Mills T.A.E. J. Allergy Clin. Immunol. 83:1055-1067(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-47.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63858 mRNA. Translation: BAA09920.1. U54781 Genomic DNA. Translation: AAA99805.1.
PIR	S68424.
3D structure databases
ProteinModelPortal	P49275.
ModBase	Search...
Protein family/group databases
Allergome	303. Der f 3. 3304. Der f 3.0101.
MEROPS	S01.234.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DERF3_DERFA

Accession

Primary (citable) accession number: P49275
Secondary accession number(s): Q23940, Q94508

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)