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P49275

- DERF3_DERFA

UniProt

P49275 - DERF3_DERFA

Protein

Mite allergen Der f 3

Gene

DERF3

Organism
Dermatophagoides farinae (American house dust mite)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei67 – 671Charge relay systemBy similarity
    Active sitei112 – 1121Charge relay systemBy similarity
    Sitei206 – 2061Required for specificity
    Active sitei212 – 2121Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.234.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mite allergen Der f 3 (EC:3.4.21.-)
    Alternative name(s):
    Allergen Der f III
    Allergen: Der f 3
    Gene namesi
    Name:DERF3
    OrganismiDermatophagoides farinae (American house dust mite)
    Taxonomic identifieri6954 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAcariAcariformesSarcoptiformesAstigmataPsoroptidiaAnalgoideaPyroglyphidaeDermatophagoidinaeDermatophagoides

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei303. Der f 3.
    3304. Der f 3.0101.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Propeptidei17 – 27111 PublicationPRO_0000028143Add
    BLAST
    Chaini28 – 259232Mite allergen Der f 3PRO_0000028144Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi52 ↔ 68PROSITE-ProRule annotation
    Disulfide bondi179 ↔ 196PROSITE-ProRule annotation
    Disulfide bondi208 ↔ 234PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP49275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 258231Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49275-1 [UniParc]FASTAAdd to Basket

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    MMILTIVVLL AANILATPIL PSSPNATIVG GVKAQAGDCP YQISLQSSSH    50
    FCGGSILDEY WILTAAHCVN GQSAKKLSIR YNTLKHASGG EKIQVAEIYQ 100
    HENYDSMTID NDVALIKLKT PMTLDQTNAK PVPLPAQGSD VKVGDKIRVS 150
    GWGYLQEGSY SLPSELQRVD IDVVSREQCD QLYSKAGADV SENMICGGDV 200
    ANGGVDSCQG DSGGPVVDVA TKQIVGIVSW GYGCARKGYP GVYTRVGNFV 250
    DWIESKRSQ 259
    Length:259
    Mass (Da):27,674
    Last modified:November 1, 1997 - v2
    Checksum:i043A62B26C4C57D7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351Q → K in AAA99805. (PubMed:8563488)Curated
    Sequence conflicti35 – 351Q → L AA sequence (PubMed:2732406)Curated
    Sequence conflicti42 – 421Q → E AA sequence (PubMed:2732406)Curated
    Sequence conflicti46 – 472QS → EV AA sequence (PubMed:2732406)Curated
    Sequence conflicti136 – 1361A → P in AAA99805. (PubMed:8563488)Curated
    Sequence conflicti219 – 2191V → I in AAA99805. (PubMed:8563488)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63858 mRNA. Translation: BAA09920.1.
    U54781 Genomic DNA. Translation: AAA99805.1.
    PIRiS68424.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63858 mRNA. Translation: BAA09920.1 .
    U54781 Genomic DNA. Translation: AAA99805.1 .
    PIRi S68424.

    3D structure databases

    ProteinModelPortali P49275.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 303. Der f 3.
    3304. Der f 3.0101.
    MEROPSi S01.234.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression in Escherichia coli of cDNA encoding house dust mite allergen Der f 3, serine protease from Dermatophagoides farinae."
      Nishiyama C., Yasuhara T., Yuuki T., Okumura Y.
      FEBS Lett. 377:62-66(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Comparative analysis of the genes encoding group 3 allergens from Dermatophagoides pteronyssinus and Dermatophagoides farinae."
      Smith W.A., Thomas W.R.
      Int. Arch. Allergy Immunol. 109:133-140(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-259.
    3. "Antigenic and structural analysis of group II allergens (Der f II and Der p II) from house dust mites (Dermatophagoides spp)."
      Heymann P.W., Chapman M.D., Aalberse R.C., Fox J.W., Platts-Mills T.A.E.
      J. Allergy Clin. Immunol. 83:1055-1067(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-47.

    Entry informationi

    Entry nameiDERF3_DERFA
    AccessioniPrimary (citable) accession number: P49275
    Secondary accession number(s): Q23940, Q94508
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3