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Protein

Mite allergen Der f 3

Gene

DERF3

Organism
Dermatophagoides farinae (American house dust mite)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Charge relay systemBy similarity
Active sitei112 – 1121Charge relay systemBy similarity
Sitei206 – 2061Required for specificity
Active sitei212 – 2121Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.234.

Names & Taxonomyi

Protein namesi
Recommended name:
Mite allergen Der f 3 (EC:3.4.21.-)
Alternative name(s):
Allergen Der f III
Allergen: Der f 3
Gene namesi
Name:DERF3
OrganismiDermatophagoides farinae (American house dust mite)
Taxonomic identifieri6954 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAcariAcariformesSarcoptiformesAstigmataPsoroptidiaAnalgoideaPyroglyphidaeDermatophagoidinaeDermatophagoides

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei303. Der f 3.
3304. Der f 3.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Propeptidei17 – 27111 PublicationPRO_0000028143Add
BLAST
Chaini28 – 259232Mite allergen Der f 3PRO_0000028144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 68PROSITE-ProRule annotation
Disulfide bondi179 ↔ 196PROSITE-ProRule annotation
Disulfide bondi208 ↔ 234PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP49275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 258231Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49275-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMILTIVVLL AANILATPIL PSSPNATIVG GVKAQAGDCP YQISLQSSSH
60 70 80 90 100
FCGGSILDEY WILTAAHCVN GQSAKKLSIR YNTLKHASGG EKIQVAEIYQ
110 120 130 140 150
HENYDSMTID NDVALIKLKT PMTLDQTNAK PVPLPAQGSD VKVGDKIRVS
160 170 180 190 200
GWGYLQEGSY SLPSELQRVD IDVVSREQCD QLYSKAGADV SENMICGGDV
210 220 230 240 250
ANGGVDSCQG DSGGPVVDVA TKQIVGIVSW GYGCARKGYP GVYTRVGNFV

DWIESKRSQ
Length:259
Mass (Da):27,674
Last modified:November 1, 1997 - v2
Checksum:i043A62B26C4C57D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351Q → K in AAA99805. (PubMed:8563488)Curated
Sequence conflicti35 – 351Q → L AA sequence (PubMed:2732406)Curated
Sequence conflicti42 – 421Q → E AA sequence (PubMed:2732406)Curated
Sequence conflicti46 – 472QS → EV AA sequence (PubMed:2732406)Curated
Sequence conflicti136 – 1361A → P in AAA99805. (PubMed:8563488)Curated
Sequence conflicti219 – 2191V → I in AAA99805. (PubMed:8563488)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63858 mRNA. Translation: BAA09920.1.
U54781 Genomic DNA. Translation: AAA99805.1.
PIRiS68424.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63858 mRNA. Translation: BAA09920.1.
U54781 Genomic DNA. Translation: AAA99805.1.
PIRiS68424.

3D structure databases

ProteinModelPortaliP49275.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei303. Der f 3.
3304. Der f 3.0101.
MEROPSiS01.234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression in Escherichia coli of cDNA encoding house dust mite allergen Der f 3, serine protease from Dermatophagoides farinae."
    Nishiyama C., Yasuhara T., Yuuki T., Okumura Y.
    FEBS Lett. 377:62-66(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Comparative analysis of the genes encoding group 3 allergens from Dermatophagoides pteronyssinus and Dermatophagoides farinae."
    Smith W.A., Thomas W.R.
    Int. Arch. Allergy Immunol. 109:133-140(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-259.
  3. "Antigenic and structural analysis of group II allergens (Der f II and Der p II) from house dust mites (Dermatophagoides spp)."
    Heymann P.W., Chapman M.D., Aalberse R.C., Fox J.W., Platts-Mills T.A.E.
    J. Allergy Clin. Immunol. 83:1055-1067(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-47.

Entry informationi

Entry nameiDERF3_DERFA
AccessioniPrimary (citable) accession number: P49275
Secondary accession number(s): Q23940, Q94508
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.