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P49275 (DERF3_DERFA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mite allergen Der f 3

EC=3.4.21.-
Alternative name(s):
Allergen Der f III
Allergen=Der f 3
Gene names
Name:DERF3
OrganismDermatophagoides farinae (American house dust mite)
Taxonomic identifier6954 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAcariAcariformesSarcoptiformesAstigmataPsoroptidiaAnalgoideaPyroglyphidaeDermatophagoidinaeDermatophagoides

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secreted.

Allergenic properties

Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Propeptide17 – 2711
PRO_0000028143
Chain28 – 259232Mite allergen Der f 3
PRO_0000028144

Regions

Domain28 – 258231Peptidase S1

Sites

Active site671Charge relay system By similarity
Active site1121Charge relay system By similarity
Active site2121Charge relay system By similarity
Site2061Required for specificity

Amino acid modifications

Disulfide bond52 ↔ 68 By similarity
Disulfide bond179 ↔ 196 By similarity
Disulfide bond208 ↔ 234 By similarity

Experimental info

Sequence conflict351Q → K in AAA99805. Ref.2
Sequence conflict351Q → L AA sequence Ref.3
Sequence conflict421Q → E AA sequence Ref.3
Sequence conflict46 – 472QS → EV AA sequence Ref.3
Sequence conflict1361A → P in AAA99805. Ref.2
Sequence conflict2191V → I in AAA99805. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P49275 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 043A62B26C4C57D7

FASTA25927,674
        10         20         30         40         50         60 
MMILTIVVLL AANILATPIL PSSPNATIVG GVKAQAGDCP YQISLQSSSH FCGGSILDEY 

        70         80         90        100        110        120 
WILTAAHCVN GQSAKKLSIR YNTLKHASGG EKIQVAEIYQ HENYDSMTID NDVALIKLKT 

       130        140        150        160        170        180 
PMTLDQTNAK PVPLPAQGSD VKVGDKIRVS GWGYLQEGSY SLPSELQRVD IDVVSREQCD 

       190        200        210        220        230        240 
QLYSKAGADV SENMICGGDV ANGGVDSCQG DSGGPVVDVA TKQIVGIVSW GYGCARKGYP 

       250 
GVYTRVGNFV DWIESKRSQ 

« Hide

References

[1]"Cloning and expression in Escherichia coli of cDNA encoding house dust mite allergen Der f 3, serine protease from Dermatophagoides farinae."
Nishiyama C., Yasuhara T., Yuuki T., Okumura Y.
FEBS Lett. 377:62-66(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Comparative analysis of the genes encoding group 3 allergens from Dermatophagoides pteronyssinus and Dermatophagoides farinae."
Smith W.A., Thomas W.R.
Int. Arch. Allergy Immunol. 109:133-140(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-259.
[3]"Antigenic and structural analysis of group II allergens (Der f II and Der p II) from house dust mites (Dermatophagoides spp)."
Heymann P.W., Chapman M.D., Aalberse R.C., Fox J.W., Platts-Mills T.A.E.
J. Allergy Clin. Immunol. 83:1055-1067(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-47.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D63858 mRNA. Translation: BAA09920.1.
U54781 Genomic DNA. Translation: AAA99805.1.
PIRS68424.

3D structure databases

ProteinModelPortalP49275.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome303. Der f 3.
3304. Der f 3.0101.
MEROPSS01.234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDERF3_DERFA
AccessionPrimary (citable) accession number: P49275
Secondary accession number(s): Q23940, Q94508
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: October 16, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Allergens

Nomenclature of allergens and list of entries