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P49268

- TXP3_APOSC

UniProt

P49268 - TXP3_APOSC

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Protein

Mu-cyrtautoxin-As1a

Gene
N/A
Organism
Apomastus schlingeri (Trap-door spider) (Aptostichus schlingeri)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The recombinant mu-cyrtautoxin-As1a potently and voltage-independently blocks voltage-gated sodium channels (Nav) of insects. It acts by pluging the outer vestibule of the channel. It acts in combination with a weak (30%) voltage-independent block of insect voltage-gated calcium (Cav) channels (low-voltage and high-voltage channels). Tested on DUM neurons, it inhibits sodium currents with an IC50 of 540 nM (and a Hill coefficient >1, reflecting an incomplete block at higher concentrations). It induces flaccid paralysis in adult Australian sheep blowfly Lucilia cuprina (PubMed:23473802). Is both paralytic and lethal, when injected into lepidopteran larvae. Is a slower acting toxin, being lethal at 24 hours, but not paralytic at 1 hour post-injection (PubMed:1440641).2 Publications

GO - Biological processi

  1. envenomation resulting in modulation of transmission of nerve impulse in other organism Source: UniProtKB
  2. envenomation resulting in negative regulation of high voltage-gated calcium channel activity in other organism Source: UniProtKB
  3. envenomation resulting in negative regulation of low voltage-gated calcium channel activity in other organism Source: UniProtKB
  4. envenomation resulting in negative regulation of voltage-gated sodium channel activity in other organism Source: UniProtKB
  5. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin, Voltage-gated sodium channel impairing toxin

Protein family/group databases

TCDBi8.B.21.1.1. the spider insecticidal neurotoxin cyrtautoxin (cyrautoxin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mu-cyrtautoxin-As1a
Short name:
Mu-CUTX-As1a
Alternative name(s):
Aptotoxin III
Short name:
Aps III
Aptotoxin-3
Short name:
Aps-3
Paralytic peptide III
Short name:
PP III
U2-cyrtautoxin-As1a
Short name:
U2-CUTX-As1a
OrganismiApomastus schlingeri (Trap-door spider) (Aptostichus schlingeri)
Taxonomic identifieri12944 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeEuctenizidaeApomastus

Organism-specific databases

ArachnoServeriAS000403. mu-cyrtautoxin-As1a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 133 pmol/g against the tobacco hornworm Manduca sexta (PubMed:1440641) and more than 2.66 pmol/g against the beet armyworm Spodoptera exigua (PubMed:1440641).1 Publication
PD50 measured 24h after injection in adult Australian sheep blowfly Lucilia cuprina is 700 +- 35 pmol/g.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3737Mu-cyrtautoxin-As1aPRO_0000044986Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi1 ↔ 151 Publication
Disulfide bondi8 ↔ 191 Publication
Disulfide bondi14 ↔ 351 Publication
Disulfide bondi26 ↔ 311 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
37
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 104Combined sources
Helixi11 – 133Combined sources
Beta strandi24 – 263Combined sources
Beta strandi28 – 303Combined sources
Beta strandi33 – 353Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M36NMR-A1-37[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the insecticidal toxin ABC family.Curated

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR012626. Spider_insecticidal_peptide.
[Graphical view]
PfamiPF08091. Toxin_21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49268-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30
CNSKGTPCTN ADECCGGKCA YNVWNCIGGG CSKTCGY
Length:37
Mass (Da):3,769
Last modified:February 1, 1996 - v1
Checksum:iCBD01091694E1908
GO

Sequence databases

PIRiE44007.

Cross-referencesi

Web resourcesi

Biological Magnetic Resonance Data Bank

Sequence databases

PIRi E44007.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2M36 NMR - A 1-37 [» ]
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 8.B.21.1.1. the spider insecticidal neurotoxin cyrtautoxin (cyrautoxin) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ArachnoServeri AS000403. mu-cyrtautoxin-As1a.

Family and domain databases

InterProi IPR012626. Spider_insecticidal_peptide.
[Graphical view ]
Pfami PF08091. Toxin_21. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of insecticidal peptides from venom of the trap-door spider, Aptostichus schlingeri (Ctenizidae)."
    Skinner W.S., Dennis P.A., Li J.P., Quistad G.B.
    Toxicon 30:1043-1050(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, BIOASSAY, LETHAL DOSE.
    Tissue: Venom.
  2. "The insecticidal neurotoxin Aps III is an atypical knottin peptide that potently blocks insect voltage-gated sodium channels."
    Bende N.S., Kang E., Herzig V., Bosmans F., Nicholson G.M., Mobli M., King G.F.
    Biochem. Pharmacol. 85:1542-1554(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, STRUCTURE BY NMR, DISULFIDE BONDS.

Entry informationi

Entry nameiTXP3_APOSC
AccessioniPrimary (citable) accession number: P49268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not modulate the activity of delayed-rectifier, "A-type" or BKCa potassium channels.1 Publication

Caution

No comparison between the natural toxin and the recombinant one has been done to attest that the results can be also attributed to the natural toxin (mode of action and disulfide bonds).1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3