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P49268

- TXP3_APOSC

UniProt

P49268 - TXP3_APOSC

Protein

Mu-cyrtautoxin-As1a

Gene
N/A
Organism
Apomastus schlingeri (Trap-door spider) (Aptostichus schlingeri)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    The recombinant mu-cyrtautoxin-As1a potently and voltage-independently blocks voltage-gated sodium channels (Nav) of insects. It acts by pluging the outer vestibule of the channel. It acts in combination with a weak (30%) voltage-independent block of insect voltage-gated calcium (Cav) channels (low-voltage and high-voltage channels). Tested on DUM neurons, it inhibits sodium currents with an IC50 of 540 nM (and a Hill coefficient >1, reflecting an incomplete block at higher concentrations). It induces flaccid paralysis in adult Australian sheep blowfly Lucilia cuprina (PubMed:23473802). Is both paralytic and lethal, when injected into lepidopteran larvae. Is a slower acting toxin, being lethal at 24 hours, but not paralytic at 1 hour post-injection (PubMed:1440641).2 Publications

    GO - Biological processi

    1. envenomation resulting in modulation of transmission of nerve impulse in other organism Source: UniProtKB
    2. envenomation resulting in negative regulation of high voltage-gated calcium channel activity in other organism Source: UniProtKB
    3. envenomation resulting in negative regulation of low voltage-gated calcium channel activity in other organism Source: UniProtKB
    4. envenomation resulting in negative regulation of voltage-gated sodium channel activity in other organism Source: UniProtKB
    5. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin, Voltage-gated sodium channel impairing toxin

    Protein family/group databases

    TCDBi8.B.21.1.1. the spider insecticidal neurotoxin cyrtautoxin (cyrautoxin) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mu-cyrtautoxin-As1a
    Short name:
    Mu-CUTX-As1a
    Alternative name(s):
    Aptotoxin III
    Short name:
    Aps III
    Aptotoxin-3
    Short name:
    Aps-3
    Paralytic peptide III
    Short name:
    PP III
    U2-cyrtautoxin-As1a
    Short name:
    U2-CUTX-As1a
    OrganismiApomastus schlingeri (Trap-door spider) (Aptostichus schlingeri)
    Taxonomic identifieri12944 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeEuctenizidaeApomastus

    Organism-specific databases

    ArachnoServeriAS000403. mu-cyrtautoxin-As1a.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Toxic dosei

    LD50 is 133 pmol/g against the tobacco hornworm Manduca sexta (PubMed:1440641) and more than 2.66 pmol/g against the beet armyworm Spodoptera exigua (PubMed:1440641).1 Publication
    PD50 measured 24h after injection in adult Australian sheep blowfly Lucilia cuprina is 700 +- 35 pmol/g.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 3737Mu-cyrtautoxin-As1aPRO_0000044986Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi1 ↔ 151 Publication
    Disulfide bondi8 ↔ 191 Publication
    Disulfide bondi14 ↔ 351 Publication
    Disulfide bondi26 ↔ 311 Publication

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    37
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 104
    Helixi11 – 133
    Beta strandi24 – 263
    Beta strandi28 – 303
    Beta strandi33 – 353

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2M36NMR-A1-37[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

    Sequence similaritiesi

    Belongs to the insecticidal toxin ABC family.Curated

    Keywords - Domaini

    Knottin

    Family and domain databases

    InterProiIPR012626. Spider_insecticidal_peptide.
    [Graphical view]
    PfamiPF08091. Toxin_21. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49268-1 [UniParc]FASTAAdd to Basket

    « Hide

    CNSKGTPCTN ADECCGGKCA YNVWNCIGGG CSKTCGY                 37
    Length:37
    Mass (Da):3,769
    Last modified:February 1, 1996 - v1
    Checksum:iCBD01091694E1908
    GO

    Sequence databases

    PIRiE44007.

    Cross-referencesi

    Web resourcesi

    Biological Magnetic Resonance Data Bank

    Sequence databases

    PIRi E44007.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2M36 NMR - A 1-37 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 8.B.21.1.1. the spider insecticidal neurotoxin cyrtautoxin (cyrautoxin) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ArachnoServeri AS000403. mu-cyrtautoxin-As1a.

    Family and domain databases

    InterProi IPR012626. Spider_insecticidal_peptide.
    [Graphical view ]
    Pfami PF08091. Toxin_21. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of insecticidal peptides from venom of the trap-door spider, Aptostichus schlingeri (Ctenizidae)."
      Skinner W.S., Dennis P.A., Li J.P., Quistad G.B.
      Toxicon 30:1043-1050(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, BIOASSAY, LETHAL DOSE.
      Tissue: Venom.
    2. "The insecticidal neurotoxin Aps III is an atypical knottin peptide that potently blocks insect voltage-gated sodium channels."
      Bende N.S., Kang E., Herzig V., Bosmans F., Nicholson G.M., Mobli M., King G.F.
      Biochem. Pharmacol. 85:1542-1554(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, STRUCTURE BY NMR, DISULFIDE BONDS.

    Entry informationi

    Entry nameiTXP3_APOSC
    AccessioniPrimary (citable) accession number: P49268
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    Does not modulate the activity of delayed-rectifier, "A-type" or BKCa potassium channels.1 Publication

    Caution

    No comparison between the natural toxin and the recombinant one has been done to attest that the results can be also attributed to the natural toxin (mode of action and disulfide bonds).1 Publication

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3