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Protein

Vesicular integral-membrane protein VIP36

Gene

LMAN2

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans.2 Publications

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96Carbohydrate1
Binding sitei131Carbohydrate1
Metal bindingi162Calcium1
Metal bindingi164Calcium; via carbonyl oxygen1
Metal bindingi166Calcium1
Binding sitei190Carbohydrate1
Metal bindingi193Calcium1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular integral-membrane protein VIP36
Alternative name(s):
Lectin mannose-binding 2
Vesicular integral-membrane protein 36
Short name:
VIP36
Gene namesi
Name:LMAN2
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 4

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini45 – 322LumenalSequence analysisAdd BLAST278
Transmembranei323 – 345HelicalSequence analysisAdd BLAST23
Topological domaini346 – 356CytoplasmicSequence analysisAdd BLAST11

GO - Cellular componenti

  • COPI-coated vesicle Source: UniProtKB
  • cytoplasmic vesicle Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment Source: GO_Central
  • endoplasmic reticulum membrane Source: GO_Central
  • ER to Golgi transport vesicle Source: GO_Central
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 441 PublicationAdd BLAST44
ChainiPRO_000001766545 – 356Vesicular integral-membrane protein VIP36Add BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi183N-linked (GlcNAc...)1 Publication1
Disulfide bondi202 ↔ 239PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP49256.
PRIDEiP49256.

Expressioni

Tissue specificityi

Expressed in kidney, liver, intestine, lung, spleen and heart. Low expression in brain.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

MINTiMINT-4655606.
STRINGi9615.ENSCAFP00000040025.

Structurei

Secondary structure

1356
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi56 – 58Combined sources3
Turni65 – 67Combined sources3
Beta strandi68 – 71Combined sources4
Beta strandi74 – 79Combined sources6
Beta strandi88 – 91Combined sources4
Beta strandi93 – 105Combined sources13
Beta strandi110 – 121Combined sources12
Beta strandi132 – 139Combined sources8
Beta strandi144 – 150Combined sources7
Beta strandi155 – 162Combined sources8
Beta strandi167 – 169Combined sources3
Beta strandi174 – 184Combined sources11
Helixi190 – 192Combined sources3
Helixi195 – 197Combined sources3
Beta strandi200 – 203Combined sources4
Beta strandi213 – 220Combined sources8
Beta strandi223 – 229Combined sources7
Beta strandi231 – 234Combined sources4
Beta strandi237 – 242Combined sources6
Beta strandi249 – 262Combined sources14
Beta strandi265 – 275Combined sources11
Helixi282 – 286Combined sources5
Helixi289 – 291Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DUOX-ray1.80A/B51-301[»]
2DUPX-ray2.10A/B51-301[»]
2DUQX-ray1.80A/B51-301[»]
2DURX-ray1.65A/B51-301[»]
2E6VX-ray2.50A/B/C/D/E51-301[»]
ProteinModelPortaliP49256.
SMRiP49256.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49256.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 276L-type lectin-likePROSITE-ProRule annotationAdd BLAST225

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 166Carbohydrate binding3
Regioni260 – 262Carbohydrate binding3

Sequence similaritiesi

Contains 1 L-type lectin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3839. Eukaryota.
ENOG410YH8V. LUCA.
GeneTreeiENSGT00530000062977.
HOGENOMiHOG000164540.
HOVERGENiHBG052334.
InParanoidiP49256.
KOiK10082.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEGWIWRW GWGRRCLGRP GLPGPGPGPA TPLFLLLLLG PVVADITDGN
60 70 80 90 100
SEHLKREHSL IKPYQGVGSS SMPLWDFQGS TILTSQYVRL TPDERSKEGS
110 120 130 140 150
IWNHQPCFLK DWEMHVHFKV HGTGKKNLHG DGIALWYTRD RLVPGPVFGS
160 170 180 190 200
KDNFHGLAIF LDTYPNDETT ERVFPYISVM VNNGSLSYDH SKDGRWTELA
210 220 230 240 250
GCTADFRNRD HDTFLAVRYS RGRLTVMTDL EDKNEWKNCI DITGVRLPTG
260 270 280 290 300
YYFGASAGTG DLSDNHDIIS MKLFQLMVEH TPDEENIDWT KIEPSVNFLK
310 320 330 340 350
SPKDNVDDPT GNFRSGPLTG WRVFLLLLCA LLGIIVCAVV GAVVFQKRQE

RNKRFY
Length:356
Mass (Da):40,214
Last modified:February 1, 1996 - v1
Checksum:iAD9646E2BCB37A85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76392 mRNA. Translation: CAA53977.1.
RefSeqiNP_001003258.1. NM_001003258.2.
UniGeneiCfa.3799.

Genome annotation databases

EnsembliENSCAFT00000026045; ENSCAFP00000024181; ENSCAFG00000016430.
GeneIDi403938.
KEGGicfa:403938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76392 mRNA. Translation: CAA53977.1.
RefSeqiNP_001003258.1. NM_001003258.2.
UniGeneiCfa.3799.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DUOX-ray1.80A/B51-301[»]
2DUPX-ray2.10A/B51-301[»]
2DUQX-ray1.80A/B51-301[»]
2DURX-ray1.65A/B51-301[»]
2E6VX-ray2.50A/B/C/D/E51-301[»]
ProteinModelPortaliP49256.
SMRiP49256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4655606.
STRINGi9615.ENSCAFP00000040025.

Proteomic databases

PaxDbiP49256.
PRIDEiP49256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000026045; ENSCAFP00000024181; ENSCAFG00000016430.
GeneIDi403938.
KEGGicfa:403938.

Organism-specific databases

CTDi10960.

Phylogenomic databases

eggNOGiKOG3839. Eukaryota.
ENOG410YH8V. LUCA.
GeneTreeiENSGT00530000062977.
HOGENOMiHOG000164540.
HOVERGENiHBG052334.
InParanoidiP49256.
KOiK10082.

Miscellaneous databases

EvolutionaryTraceiP49256.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLMAN2_CANLF
AccessioniPrimary (citable) accession number: P49256
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.