Reviewed, UniProtKB/Swiss-Prot P49253 (AOF_ONCMY)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing]
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase
      Short name=MAO

Gene names

Name:

mao

Organism

Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Taxonomic identifier

8022 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Oncorhynchus

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Oxidizes both 5-hydroxytryptamine (5-HT) and beta-phenylethylamine (PEA).
Catalytic activity	RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.
Cofactor	FAD.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the flavin monoamine oxidase family.
Sequence caution	The sequence AAA64302.1 differs from that shown. Reason: Frameshift at position 500. Probable frameshift identified on the basis of homology with other members of this family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Amine oxidase [flavin-containing]		PRO_0000099864
Regions
	Topological domain	1 – 492	492	Cytoplasmic By similarity
	Transmembrane	493 – 513	21	Anchor for type IV membrane protein By similarity
	Topological domain	514 – 522	9	Mitochondrial intermembrane By similarity
Sites
	Site	158	1	Important for catalytic activity By similarity
	Site	367	1	Important for catalytic activity By similarity
	Site	384	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	399	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49253-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: F82D6D15364D646D	FASTA	522	58,937
	10 20 30 40 50 60 MTAQNTFDVI VIGGGISGLS AAKLLKEKGL SPVVLEARDR VGGRTFTVQN EQTKYVDLGG 70 80 90 100 110 120 AYVGPTQNRI LRLAKECGVK TIKVNEEERL VHYVKGKSYP FKGSFPPMWN PFALMDYNNL 130 140 150 160 170 180 WRKMDEMGSE IPREAPWKAP HAEEWDKMTM KQLFDKICWT SSARRFATLF VNVNVTSEPH 190 200 210 220 230 240 EVSALWFLWY VKQCGGTMRI FSTTNGGQER KFLGGSSQIS ECMAKELGER VKMESPVYKI 250 260 270 280 290 300 DQTGDMVEVE TLNKETYKAK YVIVATPPGL NLKMHFNPEL PPLRNQLIHR VPMGSVIKCI 310 320 330 340 350 360 VYYRENFWRK KGYCGTMVIE EEEAPIGLTL DDTKPDGTVP AIMGFILARK CRKLCGLTKE 370 380 390 400 410 420 ERKKRICEIY SRVLGSEEAL HPVHYEEKNW CEEEYSGGCY TAYFPPGILT QYGKVLREPV 430 440 450 460 470 480 GRLYFAGTET ATEWSGYMEG AVQAGERAAR EVMYEMGRIP QSQIWQTEPE SVEVPALPFV 490 500 510 520 TTFWERNLPS VGGFINFLAA SVLSVATAAG MLAYQKGLLT RS

References

[1]	"Cloning of a novel monoamine oxidase cDNA from trout liver." Chen K., Wu H.-F., Grimsby J., Shih J.C. Mol. Pharmacol. 46:1226-1233(1994) [PubMed: 7808446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ACTIVITY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L37878 mRNA. Translation: AAA64302.1. Frameshift.
PIR	I51346.
RefSeq	NP_001118160.1.
UniGene	Omy.37002
3D structure databases
HSSP	HSSP built from PDB template 1GOS based on UniProtKB P27338.
SMR	P49253. Positions 5-503.
ModBase	Search...
Genome annotation databases
GeneID	100136729.
Phylogenomic databases
HOVERGEN	P49253.
Enzyme and pathway databases
BRENDA	1.4.3.4. 3435.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOF_ONCMY

Accession

Primary (citable) accession number: P49253

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)