Reviewed, UniProtKB/Swiss-Prot P49250 (AMO_KLEAE)

Names and origin

Protein names

Recommended name:
    Primary amine oxidase
    EC=1.4.3.21
Alternative name(s):
    Copper amine oxidase
    Monamine oxidase
    Tyramine oxidase

Gene names

Name:	maoA
Synonyms:	tynA

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Active on tyramine, tryptamine, beta-phenethylamine and dopamine.
Catalytic activity	RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.
Cofactor	Binds 1 copper ion per subunit. Contains 1 topaquinone per subunit By similarity.
Subcellular location	Periplasm.
Induction	By tyramine and catecholamines.
Post-translational modification	Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue By similarity.
Sequence similarities	Belongs to the copper/topaquinone oxidase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism
Cellular component	Periplasm
Domain	Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	TPQ
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.1
	Chain	31 – 755	725	Primary amine oxidase		PRO_0000035674
Sites
	Active site	413	1	Proton acceptor By similarity
	Active site	496	1	Schiff-base intermediate with substrate; via topaquinone By similarity
	Metal binding	554	1	Copper Potential
	Metal binding	556	1	Copper Potential
	Metal binding	719	1	Copper Potential
Amino acid modifications
	Modified residue	496	1	2',4',5'-topaquinone By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49250-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7B5552283CD93EFF	FASTA	755	83,577
	10 20 30 40 50 60 MANGLKFSPR KTALALAVAV VCAWQSPVFA HGSEAHMVPL DKTLQEFGAD VQWDDYAQMF 70 80 90 100 110 120 TLIKDGAYVK VKPGAKTAIV NGKSLDLPVP VVMKEGKAWV SDTFINDVFQ SGLDQTFQVE 130 140 150 160 170 180 KRPHPLNSLS AAEISKAVTI VKAAPEFQPN TRFTEISLHE PDKAAVWAFA LQGTPVDAPR 190 200 210 220 230 240 TADVVMLDGK HVIEAVVDLQ NKKILSWTPI KGAHGMVLLD DFVSVQNIIN TSSEFAEVLK 250 260 270 280 290 300 KHGITDPGKV VTTPLTVGFF DGKDGLQQDA RLLKVVSYLD TGDGNYWAHP IENLVAVVDL 310 320 330 340 350 360 EAKKIIKIEE GPVIPVPMEP RPYDGRDRNA PAVKPLEITE PEGKNYTITG DTIHWQNWDF 370 380 390 400 410 420 HLRLNSRVGP ILSTVTYNDN GTKRQVMYEG SLGGMIVPYG DPDVGWYFKA YLDSGDYGMG 430 440 450 460 470 480 TLTSPIVRGK DAPSNAVLLD ETIADYTGKP TTIPGAVAIF ERYAGPEYKH LEMGKPNVST 490 500 510 520 530 540 ERRELVVRWI STVGNYDYIF DWVFHDNGTI GIDAGATGIE AVKGVLAKTM HDPSAKEDTR 550 560 570 580 590 600 YGTLIDHNIV GTTHQHIYNF RLDLDVDGEN NTLVAMDPEV KPNTAGGPRT STMQVNQYTI 610 620 630 640 650 660 DSEQKAAQKF DPGTIRLLSN TSKENRMGNP VSYQIIPYAG GTHPAATGAK FAPDEWIYHR 670 680 690 700 710 720 LSFMDKQLWV TRYHPTERYP EGKYPNRSAH DTGLGQYAKD DESLTNHDDV VWITTGTTHV 730 740 750 ARAEEWPIMP TEWALALLKP WNFFDETPTL GEKKK

References

[1]

"A monoamine-regulated Klebsiella aerogenes operon containing the monoamine oxidase structural gene (maoA) and the maoC gene." Sugino H., Sasaki M., Azakami H., Yamashita M., Murooka Y. J. Bacteriol. 174:2485-2492(1992) [PubMed: 1556068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-41. Strain: W70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10208 Genomic DNA. Translation: BAA01060.1.
3D structure databases
HSSP	HSSP built from PDB template 1OAC based on UniProtKB P46883.
SMR	P49250. Positions 35-754.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.3.6. 366.
Family and domain databases
InterPro	IPR000269. Cu_amine_oxidase. IPR012854. Cu_amine_oxidase-like_N. IPR015798. Cu_amine_oxidase_C. IPR015800. Cu_amine_oxidase_N2. IPR015801. Cu_amine_oxidase_N2/3. IPR015802. Cu_amine_oxidase_N3. [Graphical view]
Gene3D	G3DSA:3.30.457.10. Cu_amine_oxidase-like_N. 1 hit. G3DSA:3.10.450.40. CuNH_oxidase. 2 hits. G3DSA:2.70.98.20. Lyase_8_central. 1 hit.
PANTHER	PTHR10638. CuNH_oxidase. 1 hit.
Pfam	PF01179. Cu_amine_oxid. 1 hit. PF07833. Cu_amine_oxidN1. 1 hit. PF02727. Cu_amine_oxidN2. 1 hit. PF02728. Cu_amine_oxidN3. 1 hit. [Graphical view]
PROSITE	PS01164. COPPER_AMINE_OXID_1. 1 hit. PS01165. COPPER_AMINE_OXID_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMO_KLEAE

Accession

Primary (citable) accession number: P49250

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)