P49243 (FABH_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase III, chloroplastic
EC=2.3.1.180

Alternative name(s):
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Ordered Locus Names:	At1g62640
ORF Names:	T3P18.20, F23N19.2

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. KAS III catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities By similarity.
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the FabH family.
Sequence caution	The sequence AAD43621.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAF19534.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 74	74	Chloroplast Potential
	Chain	75 – 404	330	3-oxoacyl-[acyl-carrier-protein] synthase III, chloroplastic		PRO_0000008732
Sites
	Active site	179	1	By similarity
	Active site	330	1	By similarity
	Active site	360	1	By similarity
Experimental info
	Sequence conflict	108	1	N → T in AAA61348. Ref.1
	Sequence conflict	114	1	G → A in AAA61348. Ref.1
	Sequence conflict	190	1	A → D in AAA61348. Ref.1
	Sequence conflict	266 – 268	3	QND → RNE in AAA61348. Ref.1
	Sequence conflict	343	1	R → S in AAA61348. Ref.1
	Sequence conflict	401	1	M → V in AAA61348. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49243 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: 72D49DFE4044B44E	FASTA	404	42,847
	10 20 30 40 50 60 MANASGFFTH PSIPNLRSRI HVPVRVSGSG FCVSNRFSKR VLCSSVSSVD KDASSSPSQY 70 80 90 100 110 120 QRPRLVPSGC KLIGCGSAVP SLLISNDDLA KIVDTNDEWI ATRTGIRNRR VVSGKDSLVG 130 140 150 160 170 180 LAVEAATKAL EMAEVVPEDI DLVLMCTSTP DDLFGAAPQI QKALGCTKNP LAYDITAACS 190 200 210 220 230 240 GFVLGLVSAA CHIRGGGFKN VLVIGADSLS RFVDWTDRGT CILFGDAAGA VVVQACDIED 250 260 270 280 290 300 DGLFSFDVHS DGDGRRHLNA SVKESQNDGE SSSNGSVFGD FPPKQSSYSC IQMNGKEVFR 310 320 330 340 350 360 FAVKCVPQSI ESALQKAGLP ASAIDWLLLH QANQRIIDSV ATRLHFPPER VISNLANYGN 370 380 390 400 TSAASIPLAL DEAVRSGKVK PGHTIATSGF GAGLTWGSAI MRWR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding 3-ketoacyl-acyl carrier protein synthase III from Arabidopsis." Tai H., Post-Beittenmiller D., Jaworski J.G. Plant Physiol. 106:801-802(1994) [PubMed: 7991698] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Cloning of a gene encoding 3-ketoacyl-acyl carrier protein synthase III from Arabidopsis thaliana." Haouazine-Takvorian N., Takvorian A., Kreis M. Plant Gene Register PGR97-149 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L31891 mRNA. Translation: AAA61348.1. Y11689 Genomic DNA. Translation: CAA72385.1. AC005698 Genomic DNA. Translation: AAD43621.1. Sequence problems. AC007190 Genomic DNA. Translation: AAF19534.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE33989.1. CP002684 Genomic DNA. Translation: AEE33990.1. AY063804 mRNA. Translation: AAL36160.1. AY091275 mRNA. Translation: AAM14214.1.
IPI	IPI00539872.
RefSeq	NP_001031221.1. NM_001036144.1. NP_176452.1. NM_104942.3.
UniGene	At.386.
3D structure databases
ProteinModelPortal	P49243.
SMR	P49243. Positions 69-403.
ModBase	Search...
Protein-protein interaction databases
STRING	P49243.
Proteomic databases
PRIDE	P49243.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G62640.1; AT1G62640.1; AT1G62640. AT1G62640.2; AT1G62640.2; AT1G62640.
GeneID	842561.
GenomeReviews	Gene locus AT1G62640 in contig CT485782_GR.
KEGG	ath:AT1G62640.
NMPDR	fig|3702.1.peg.5656.
Organism-specific databases
TAIR	At1g62640.
Phylogenomic databases
eggNOG	euNOG15867.
GeneTree	EPGT00050000004329.
HOGENOM	HBG649927.
InParanoid	P49243.
OMA	FNAKEEA.
PhylomeDB	P49243.
ProtClustDB	PLN02326.
Gene expression databases
ArrayExpress	P49243.
Genevestigator	P49243.
GermOnline	AT1G62640. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 3 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_ARATH

Accession

Primary (citable) accession number: P49243
Secondary accession number(s): P93722, Q9SI84, Q9SXD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 10, 2003
Last modified:	January 25, 2012
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program