Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P49236

- E13B_BRACM

UniProt

P49236 - E13B_BRACM

Protein

Glucan endo-1,3-beta-glucosidase

Gene

BGL

Organism
Brassica campestris (Field mustard)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Is thought to be an important plant defense-related product against fungal pathogens. Accumulation of the glucanase can be detected as early as 4 hours after inoculation.

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei261 – 2611NucleophileBy similarity
    Active sitei322 – 3221Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase
    Short name:
    (1->3)-beta-glucanase
    Beta-1,3-endoglucanase
    Gene namesi
    Name:BGL
    OrganismiBrassica campestris (Field mustard)
    Taxonomic identifieri3711 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    Vacuole
    Note: In intact tissues.

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626By similarityAdd
    BLAST
    Chaini27 – 342316Glucan endo-1,3-beta-glucosidasePRO_0000011842Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Expressioni

    Inductioni

    Following incompatible or compatible interaction with pathogenic bacteria, but not by wounding or salicylic acid. The induction is localized and does not seem to result from a systemic response. Induction occurs more rapidly with an incompatible interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP49236.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49236-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLASSPMLLF LLSLLMAYNF DTTAGQIGVC FGQMGNNIPN PSEVVAMFKQ    50
    YSIPRMRMYG PNPDALNALR GSNIEFILDV PNGDLKRLAD SQAEANTWVR 100
    DNVQKYNDVR FKYISVGNEV KPGEPGAAAL IQAMQNIDRA LSAAGLSNIK 150
    VSTTTFMGPS RNTYPPSRGR FKDEYRNFLQ PVIGFLVNKR SPLLVNIYTY 200
    FGYMNRDVSL QFALLQPNSN NEFTDPNNQL RYLNFFDANL DSVYAALEKS 250
    GGGSLDVVVS ESGWPTQGGP GASVPNAEAY VNNLRLHVNK NGSPKRQEAI 300
    ETYIFAMFDE APRQTSPNDE YEKYWGMFSP TTRQLKYGVK FN 342
    Length:342
    Mass (Da):38,180
    Last modified:February 1, 1996 - v1
    Checksum:i7AE6B1EA445957F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77990 mRNA. Translation: CAA54952.1.
    PIRiS42885.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77990 mRNA. Translation: CAA54952.1 .
    PIRi S42885.

    3D structure databases

    ProteinModelPortali P49236.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Defense-related gene induction in Brassica campestris in response to defined mutants of Xanthomonas campestris with altered pathogenicity."
      Newman M.-A., Conrads-Strauch J., Scofield G., Daniels M.J., Dow J.M.
      Mol. Plant Microbe Interact. 7:553-563(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 99-117.
      Strain: cv. Just Right.
      Tissue: Leaf.

    Entry informationi

    Entry nameiE13B_BRACM
    AccessioniPrimary (citable) accession number: P49236
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3