P49236 (E13B_BRACM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase

Gene names

Name:

BGL

Organism

Brassica campestris (Field mustard)

Taxonomic identifier

3711 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens. Accumulation of the glucanase can be detected as early as 4 hours after inoculation.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole. Note: In intact tissues.
Induction	Following incompatible or compatible interaction with pathogenic bacteria, but not by wounding or salicylic acid. The induction is localized and does not seem to result from a systemic response. Induction occurs more rapidly with an incompatible interaction.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	By similarity
	Chain	27 – 342	316	Glucan endo-1,3-beta-glucosidase		PRO_0000011842
Sites
	Active site	261	1	Nucleophile By similarity
	Active site	322	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49236 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7AE6B1EA445957F7	FASTA	342	38,180
	10 20 30 40 50 60 MLASSPMLLF LLSLLMAYNF DTTAGQIGVC FGQMGNNIPN PSEVVAMFKQ YSIPRMRMYG 70 80 90 100 110 120 PNPDALNALR GSNIEFILDV PNGDLKRLAD SQAEANTWVR DNVQKYNDVR FKYISVGNEV 130 140 150 160 170 180 KPGEPGAAAL IQAMQNIDRA LSAAGLSNIK VSTTTFMGPS RNTYPPSRGR FKDEYRNFLQ 190 200 210 220 230 240 PVIGFLVNKR SPLLVNIYTY FGYMNRDVSL QFALLQPNSN NEFTDPNNQL RYLNFFDANL 250 260 270 280 290 300 DSVYAALEKS GGGSLDVVVS ESGWPTQGGP GASVPNAEAY VNNLRLHVNK NGSPKRQEAI 310 320 330 340 ETYIFAMFDE APRQTSPNDE YEKYWGMFSP TTRQLKYGVK FN

References

[1]

"Defense-related gene induction in Brassica campestris in response to defined mutants of Xanthomonas campestris with altered pathogenicity." Newman M.-A., Conrads-Strauch J., Scofield G., Daniels M.J., Dow J.M. Mol. Plant Microbe Interact. 7:553-563(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 99-117. Strain: cv. Just Right. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77990 mRNA. Translation: CAA54952.1.
PIR	S42885.
3D structure databases
ProteinModelPortal	P49236.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_BRACM

Accession

Primary (citable) accession number: P49236

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program