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P49236

- E13B_BRACM

UniProt

P49236 - E13B_BRACM

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Protein

Glucan endo-1,3-beta-glucosidase

Gene

BGL

Organism
Brassica campestris (Field mustard)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Is thought to be an important plant defense-related product against fungal pathogens. Accumulation of the glucanase can be detected as early as 4 hours after inoculation.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei261 – 2611NucleophileBy similarity
Active sitei322 – 3221Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase
Gene namesi
Name:BGL
OrganismiBrassica campestris (Field mustard)
Taxonomic identifieri3711 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Vacuole
Note: In intact tissues.

GO - Cellular componenti

  1. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626By similarityAdd
BLAST
Chaini27 – 342316Glucan endo-1,3-beta-glucosidasePRO_0000011842Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Expressioni

Inductioni

Following incompatible or compatible interaction with pathogenic bacteria, but not by wounding or salicylic acid. The induction is localized and does not seem to result from a systemic response. Induction occurs more rapidly with an incompatible interaction.

Structurei

3D structure databases

ProteinModelPortaliP49236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49236-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLASSPMLLF LLSLLMAYNF DTTAGQIGVC FGQMGNNIPN PSEVVAMFKQ
60 70 80 90 100
YSIPRMRMYG PNPDALNALR GSNIEFILDV PNGDLKRLAD SQAEANTWVR
110 120 130 140 150
DNVQKYNDVR FKYISVGNEV KPGEPGAAAL IQAMQNIDRA LSAAGLSNIK
160 170 180 190 200
VSTTTFMGPS RNTYPPSRGR FKDEYRNFLQ PVIGFLVNKR SPLLVNIYTY
210 220 230 240 250
FGYMNRDVSL QFALLQPNSN NEFTDPNNQL RYLNFFDANL DSVYAALEKS
260 270 280 290 300
GGGSLDVVVS ESGWPTQGGP GASVPNAEAY VNNLRLHVNK NGSPKRQEAI
310 320 330 340
ETYIFAMFDE APRQTSPNDE YEKYWGMFSP TTRQLKYGVK FN
Length:342
Mass (Da):38,180
Last modified:February 1, 1996 - v1
Checksum:i7AE6B1EA445957F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77990 mRNA. Translation: CAA54952.1.
PIRiS42885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77990 mRNA. Translation: CAA54952.1 .
PIRi S42885.

3D structure databases

ProteinModelPortali P49236.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Defense-related gene induction in Brassica campestris in response to defined mutants of Xanthomonas campestris with altered pathogenicity."
    Newman M.-A., Conrads-Strauch J., Scofield G., Daniels M.J., Dow J.M.
    Mol. Plant Microbe Interact. 7:553-563(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 99-117.
    Strain: cv. Just Right.
    Tissue: Leaf.

Entry informationi

Entry nameiE13B_BRACM
AccessioniPrimary (citable) accession number: P49236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3