SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P49236

- E13B_BRACM

UniProt

P49236 - E13B_BRACM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glucan endo-1,3-beta-glucosidase
Gene
BGL
Organism
Brassica campestris (Field mustard)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Is thought to be an important plant defense-related product against fungal pathogens. Accumulation of the glucanase can be detected as early as 4 hours after inoculation.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei261 – 2611Nucleophile By similarity
Active sitei322 – 3221Proton donor By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase
Gene namesi
Name:BGL
OrganismiBrassica campestris (Field mustard)
Taxonomic identifieri3711 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Vacuole
Note: In intact tissues.

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 By similarity
Add
BLAST
Chaini27 – 342316Glucan endo-1,3-beta-glucosidase
PRO_0000011842Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Expressioni

Inductioni

Following incompatible or compatible interaction with pathogenic bacteria, but not by wounding or salicylic acid. The induction is localized and does not seem to result from a systemic response. Induction occurs more rapidly with an incompatible interaction.

Structurei

3D structure databases

ProteinModelPortaliP49236.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49236-1 [UniParc]FASTAAdd to Basket

« Hide

MLASSPMLLF LLSLLMAYNF DTTAGQIGVC FGQMGNNIPN PSEVVAMFKQ    50
YSIPRMRMYG PNPDALNALR GSNIEFILDV PNGDLKRLAD SQAEANTWVR 100
DNVQKYNDVR FKYISVGNEV KPGEPGAAAL IQAMQNIDRA LSAAGLSNIK 150
VSTTTFMGPS RNTYPPSRGR FKDEYRNFLQ PVIGFLVNKR SPLLVNIYTY 200
FGYMNRDVSL QFALLQPNSN NEFTDPNNQL RYLNFFDANL DSVYAALEKS 250
GGGSLDVVVS ESGWPTQGGP GASVPNAEAY VNNLRLHVNK NGSPKRQEAI 300
ETYIFAMFDE APRQTSPNDE YEKYWGMFSP TTRQLKYGVK FN 342
Length:342
Mass (Da):38,180
Last modified:February 1, 1996 - v1
Checksum:i7AE6B1EA445957F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77990 mRNA. Translation: CAA54952.1.
PIRiS42885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77990 mRNA. Translation: CAA54952.1 .
PIRi S42885.

3D structure databases

ProteinModelPortali P49236.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Defense-related gene induction in Brassica campestris in response to defined mutants of Xanthomonas campestris with altered pathogenicity."
    Newman M.-A., Conrads-Strauch J., Scofield G., Daniels M.J., Dow J.M.
    Mol. Plant Microbe Interact. 7:553-563(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 99-117.
    Strain: cv. Just Right.
    Tissue: Leaf.

Entry informationi

Entry nameiE13B_BRACM
AccessioniPrimary (citable) accession number: P49236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi