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Protein

50S ribosomal protein L32

Gene

rpmF

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Forms a cluster with L17 and L22, and with L22, a pair of "tweezers" that hold together all the domains of the 23S rRNA. Interacts with the antibiotic troleandomycin which blocks the peptide exit tunnel.

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi33 – 331ZincCurated
Metal bindingi36 – 361ZincCurated
Metal bindingi46 – 461ZincCurated
Metal bindingi49 – 491ZincCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri33 – 4917C4-typeCuratedAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

Metal-binding, RNA-binding, rRNA-binding, Zinc

Enzyme and pathway databases

BioCyciDRAD243230:GH46-2415-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L32
Gene namesi
Name:rpmF
Ordered Locus Names:DR_2366
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry

DrugBankiDB01361. Troleandomycin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 605950S ribosomal protein L32PRO_0000172336Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Contacts proteins L17 and L22.6 Publications

Protein-protein interaction databases

STRINGi243230.DR_2366.

Structurei

Secondary structure

1
60
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 197Combined sources
Turni20 – 223Combined sources
Beta strandi30 – 323Combined sources
Beta strandi34 – 363Combined sources
Beta strandi38 – 403Combined sources
Turni47 – 493Combined sources
Beta strandi51 – 555Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J5AX-ray3.50M1-60[»]
1JZXX-ray3.10M1-60[»]
1JZYX-ray3.50M1-60[»]
1JZZX-ray3.80M1-60[»]
1K01X-ray3.50M1-60[»]
1NKWX-ray3.10Z1-60[»]
1NWXX-ray3.50Z2-60[»]
1NWYX-ray3.30Z2-60[»]
1ONDX-ray3.40Z1-60[»]
1SM1X-ray3.42Z1-60[»]
1XBPX-ray3.50Z2-60[»]
2ZJPX-ray3.70Y1-60[»]
2ZJQX-ray3.30Z1-60[»]
2ZJRX-ray2.91Z1-60[»]
3CF5X-ray3.30Y1-60[»]
3DLLX-ray3.50Y1-60[»]
3PIOX-ray3.25Z1-60[»]
3PIPX-ray3.45Z1-60[»]
4IO9X-ray3.20Z1-60[»]
4IOAX-ray3.20Z1-60[»]
4IOCX-ray3.60Z1-60[»]
4U67X-ray3.65Z1-60[»]
4V49X-ray8.70Z2-59[»]
4V4AX-ray9.50Z2-59[»]
4V4GX-ray11.5022-59[»]
4V4TX-ray6.4651-60[»]
4WFNX-ray3.54Z1-60[»]
5DM6X-ray2.90Z3-59[»]
5DM7X-ray3.00Z3-59[»]
ProteinModelPortaliP49228.
SMRiP49228. Positions 2-59.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49228.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L32P family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri33 – 4917C4-typeCuratedAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG41067VY. Bacteria.
COG0333. LUCA.
HOGENOMiHOG000040268.
InParanoidiP49228.
KOiK02911.
OMAiELKLAHH.
OrthoDBiPOG091H01TG.

Family and domain databases

HAMAPiMF_00340. Ribosomal_L32. 1 hit.
InterProiIPR002677. Ribosomal_L32p.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamiPF01783. Ribosomal_L32p. 1 hit.
[Graphical view]
SUPFAMiSSF57829. SSF57829. 1 hit.
TIGRFAMsiTIGR01031. rpmF_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKHPVPKKK TSKSKRDMRR SHHALTAPNL TECPQCHGKK LSHHICPNCG
60
YYDGRQVLAV
Length:60
Mass (Da):6,792
Last modified:January 23, 2007 - v3
Checksum:i23D9FAEA1A00BC0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63900 Genomic DNA. Translation: BAA09939.1.
AE000513 Genomic DNA. Translation: AAF11913.1.
PIRiF75282.
RefSeqiNP_296087.1. NC_001263.1.
WP_010888992.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF11913; AAF11913; DR_2366.
GeneIDi1800201.
KEGGidra:DR_2366.
PATRICi21632446. VBIDeiRad64572_2600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63900 Genomic DNA. Translation: BAA09939.1.
AE000513 Genomic DNA. Translation: AAF11913.1.
PIRiF75282.
RefSeqiNP_296087.1. NC_001263.1.
WP_010888992.1. NC_001263.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J5AX-ray3.50M1-60[»]
1JZXX-ray3.10M1-60[»]
1JZYX-ray3.50M1-60[»]
1JZZX-ray3.80M1-60[»]
1K01X-ray3.50M1-60[»]
1NKWX-ray3.10Z1-60[»]
1NWXX-ray3.50Z2-60[»]
1NWYX-ray3.30Z2-60[»]
1ONDX-ray3.40Z1-60[»]
1SM1X-ray3.42Z1-60[»]
1XBPX-ray3.50Z2-60[»]
2ZJPX-ray3.70Y1-60[»]
2ZJQX-ray3.30Z1-60[»]
2ZJRX-ray2.91Z1-60[»]
3CF5X-ray3.30Y1-60[»]
3DLLX-ray3.50Y1-60[»]
3PIOX-ray3.25Z1-60[»]
3PIPX-ray3.45Z1-60[»]
4IO9X-ray3.20Z1-60[»]
4IOAX-ray3.20Z1-60[»]
4IOCX-ray3.60Z1-60[»]
4U67X-ray3.65Z1-60[»]
4V49X-ray8.70Z2-59[»]
4V4AX-ray9.50Z2-59[»]
4V4GX-ray11.5022-59[»]
4V4TX-ray6.4651-60[»]
4WFNX-ray3.54Z1-60[»]
5DM6X-ray2.90Z3-59[»]
5DM7X-ray3.00Z3-59[»]
ProteinModelPortaliP49228.
SMRiP49228. Positions 2-59.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_2366.

Chemistry

DrugBankiDB01361. Troleandomycin.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF11913; AAF11913; DR_2366.
GeneIDi1800201.
KEGGidra:DR_2366.
PATRICi21632446. VBIDeiRad64572_2600.

Phylogenomic databases

eggNOGiENOG41067VY. Bacteria.
COG0333. LUCA.
HOGENOMiHOG000040268.
InParanoidiP49228.
KOiK02911.
OMAiELKLAHH.
OrthoDBiPOG091H01TG.

Enzyme and pathway databases

BioCyciDRAD243230:GH46-2415-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP49228.

Family and domain databases

HAMAPiMF_00340. Ribosomal_L32. 1 hit.
InterProiIPR002677. Ribosomal_L32p.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamiPF01783. Ribosomal_L32p. 1 hit.
[Graphical view]
SUPFAMiSSF57829. SSF57829. 1 hit.
TIGRFAMsiTIGR01031. rpmF_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRL32_DEIRA
AccessioniPrimary (citable) accession number: P49228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.