Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P49222 (EPB42_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Erythrocyte membrane protein band 4.2

Short name=Erythrocyte protein 4.2
Short name=P4.2
Gene names
Name:Epb42
Synonyms:Epb4.2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probably plays an important role in the regulation of erythrocyte shape and mechanical properties.

Subunit structure

Oligomer. Interacts with the cytoplasmic domain of SLC4A1/band 3 anion transport protein.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmcytoskeleton. Note: Cytoplasmic surface of erythrocyte membranes.

Miscellaneous

The substitution of an Ala for a Cys in the active site may be responsible for the lack of transglutaminase activity of band 4.2.

Sequence similarities

Belongs to the transglutaminase superfamily. Transglutaminase family.

Caution

Was originally (Ref.2) thought to be pallidin.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 691690Erythrocyte membrane protein band 4.2
PRO_0000213721

Regions

Region31 – 399Band 3 binding By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine By similarity

Experimental info

Sequence conflict221Y → H in AAA67917. Ref.2
Sequence conflict2241K → N in AAA67917. Ref.2
Sequence conflict3981C → S in AAA67917. Ref.2
Sequence conflict4501K → R in AAA67917. Ref.2
Sequence conflict5281S → R in AAA67917. Ref.2
Sequence conflict6211C → S in AAA62275. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P49222 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 433E1623971D60C3

FASTA69176,756
        10         20         30         40         50         60 
MGQALSIKSC DFHAAENNEE HYTKAISSQH LTLRRGQSFT ITLNFRAPTH TFLSALKKVA 

        70         80         90        100        110        120 
LIAQTGEQPS KINKTQAIFP ISSLGDQKGW SAAVEERDAQ HWTVSVTTPV DAVIGHYSLL 

       130        140        150        160        170        180 
LQVSGKKQYP LGQFTLLFNP WNRDDAVFLQ NEAERTEYVL NQNGFIYLGT ADCIQEEPWD 

       190        200        210        220        230        240 
FGQFEKDVMD LSLKLLSMDK QVKDWNQPAH VARVVGALLH ALKKKSVLPI SQTQAAQEGA 

       250        260        270        280        290        300 
LLYKRRGSVP ILRQWLTGQG RAVYETQAWV SAAVACTVLR CLGIPARVVT TFDSAQGTVG 

       310        320        330        340        350        360 
SLLVDEYYNE EGLQNGEGQR GHIWVFQTSV ECWMNRPDLS QGYGGWQILH PRAPNGAGVL 

       370        380        390        400        410        420 
GSCSLVPVRA VKEGELQLDP AVPELFAAVN ASCVVWKCCE DGKLELTNSN RKDVGNCIST 

       430        440        450        460        470        480 
KVVGSDRCED ITQNYKYPAG SLQEKEVLEK VQKERLKLGK DNGMCPPSCE PWDPLHMFFE 

       490        500        510        520        530        540 
ASSSIPLSGD GQLSVTLINP TDEEKKVHLV IGAQALYYNG VLAAGLWSKK QLFMLKPNQV 

       550        560        570        580        590        600 
MRLSTNLSFS CFEQTPPENS FLRVTAMARY SHTSLSCFAQ ENMAIGKPDL IIEMPKRAAQ 

       610        620        630        640        650        660 
YRPLTVSVRM HNSLEAPMQN CIISIFGRGL IHREKRYGLG SLWPGSSLHT QFQFTPTHLG 

       670        680        690 
LQRLTVEVDC DMFQNLTGYR SVLVVAPEVS V 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of mouse erythrocyte protein 4.2: a membrane protein with strong homology with the transglutaminase supergene family."
Rybicki A.C., Schwartz R.S., Qiu J.J.-H., Gilman J.G.
Mamm. Genome 5:438-445(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
Tissue: Reticulocyte.
[2]"cDNA sequence, gene sequence, and properties of murine pallidin (band 4.2), the protein implicated in the murine pallid mutation."
Korsgren C., Cohen C.M.
Genomics 21:478-485(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: BALB/c and C57BL/6J.
Tissue: Liver and Reticulocyte.
[3]"Murine erythrocyte protein 4.2 gene: similarity and differences in structure and expression from its human counterpart."
Karacay B.B.K., Enzhong X.E.X., Chang L.-S.L.S.
Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Blood.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Spleen.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U03487 mRNA. Translation: AAA62275.1.
U04055 mRNA. Translation: AAA67916.1.
U04056 Genomic DNA. Translation: AAA67917.1.
L35933 mRNA. Translation: AAA39875.1.
AK143841 mRNA. Translation: BAE25564.1.
AL844548 Genomic DNA. Translation: CAM19015.1.
CCDSCCDS16631.1.
PIRA54741.
RefSeqNP_038541.1. NM_013513.2.
UniGeneMm.240051.

3D structure databases

ProteinModelPortalP49222.
SMRP49222. Positions 5-684.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP49222. 2 interactions.
MINTMINT-4094485.

PTM databases

PhosphoSiteP49222.

Proteomic databases

MaxQBP49222.
PaxDbP49222.
PRIDEP49222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102490; ENSMUSP00000099548; ENSMUSG00000023216.
GeneID13828.
KEGGmmu:13828.
UCSCuc008lxi.1. mouse.

Organism-specific databases

CTD13828.
MGIMGI:95402. Epb4.2.

Phylogenomic databases

eggNOGNOG87163.
GeneTreeENSGT00740000115034.
HOGENOMHOG000231695.
HOVERGENHBG106048.
InParanoidQ3UP33.
OMASCFAQED.
OrthoDBEOG7Z0JVS.
TreeFamTF324278.

Gene expression databases

ArrayExpressP49222.
BgeeP49222.
CleanExMM_EPB4.2.
GenevestigatorP49222.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERPTHR11590. PTHR11590. 1 hit.
PfamPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFPIRSF000459. TGM_EBP42. 1 hit.
SMARTSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284632.
PROP49222.
SOURCESearch...

Entry information

Entry nameEPB42_MOUSE
AccessionPrimary (citable) accession number: P49222
Secondary accession number(s): Q3UP33
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot