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Protein

Erythrocyte membrane protein band 4.2

Gene

Epb42

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably plays an important role in the regulation of erythrocyte shape and mechanical properties.

GO - Molecular functioni

GO - Biological processi

  • cell morphogenesis Source: MGI
  • erythrocyte maturation Source: UniProtKB-KW
  • hemoglobin metabolic process Source: MGI
  • ion homeostasis Source: MGI
  • iron ion homeostasis Source: MGI
  • peptide cross-linking Source: InterPro
  • regulation of cell shape Source: UniProtKB-KW
  • spleen development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell shape, Erythrocyte maturation

Names & Taxonomyi

Protein namesi
Recommended name:
Erythrocyte membrane protein band 4.2
Short name:
Erythrocyte protein 4.2
Short name:
P4.2
Gene namesi
Name:Epb42
Synonyms:Epb4.2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95402. Epb42.

Subcellular locationi

GO - Cellular componenti

  • cortical cytoskeleton Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002137212 – 691Erythrocyte membrane protein band 4.2Add BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei570PhosphotyrosineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP49222.
PaxDbiP49222.
PeptideAtlasiP49222.
PRIDEiP49222.

PTM databases

iPTMnetiP49222.
PhosphoSitePlusiP49222.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023216.
CleanExiMM_EPB4.2.
ExpressionAtlasiP49222. baseline and differential.
GenevisibleiP49222. MM.

Interactioni

Subunit structurei

Oligomer. Interacts with the cytoplasmic domain of SLC4A1/band 3 anion transport protein.

Protein-protein interaction databases

IntActiP49222. 2 interactors.
MINTiMINT-4094485.
STRINGi10090.ENSMUSP00000099548.

Structurei

3D structure databases

ProteinModelPortaliP49222.
SMRiP49222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 39Band 3 bindingBy similarity9

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IH88. Eukaryota.
ENOG410YC7E. LUCA.
GeneTreeiENSGT00760000119108.
HOGENOMiHOG000231695.
HOVERGENiHBG106048.
InParanoidiP49222.
OMAiSCFAQED.
OrthoDBiEOG091G030K.
TreeFamiTF324278.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQALSIKSC DFHAAENNEE HYTKAISSQH LTLRRGQSFT ITLNFRAPTH
60 70 80 90 100
TFLSALKKVA LIAQTGEQPS KINKTQAIFP ISSLGDQKGW SAAVEERDAQ
110 120 130 140 150
HWTVSVTTPV DAVIGHYSLL LQVSGKKQYP LGQFTLLFNP WNRDDAVFLQ
160 170 180 190 200
NEAERTEYVL NQNGFIYLGT ADCIQEEPWD FGQFEKDVMD LSLKLLSMDK
210 220 230 240 250
QVKDWNQPAH VARVVGALLH ALKKKSVLPI SQTQAAQEGA LLYKRRGSVP
260 270 280 290 300
ILRQWLTGQG RAVYETQAWV SAAVACTVLR CLGIPARVVT TFDSAQGTVG
310 320 330 340 350
SLLVDEYYNE EGLQNGEGQR GHIWVFQTSV ECWMNRPDLS QGYGGWQILH
360 370 380 390 400
PRAPNGAGVL GSCSLVPVRA VKEGELQLDP AVPELFAAVN ASCVVWKCCE
410 420 430 440 450
DGKLELTNSN RKDVGNCIST KVVGSDRCED ITQNYKYPAG SLQEKEVLEK
460 470 480 490 500
VQKERLKLGK DNGMCPPSCE PWDPLHMFFE ASSSIPLSGD GQLSVTLINP
510 520 530 540 550
TDEEKKVHLV IGAQALYYNG VLAAGLWSKK QLFMLKPNQV MRLSTNLSFS
560 570 580 590 600
CFEQTPPENS FLRVTAMARY SHTSLSCFAQ ENMAIGKPDL IIEMPKRAAQ
610 620 630 640 650
YRPLTVSVRM HNSLEAPMQN CIISIFGRGL IHREKRYGLG SLWPGSSLHT
660 670 680 690
QFQFTPTHLG LQRLTVEVDC DMFQNLTGYR SVLVVAPEVS V
Length:691
Mass (Da):76,756
Last modified:July 27, 2011 - v3
Checksum:i433E1623971D60C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22Y → H in AAA67917 (PubMed:7959722).Curated1
Sequence conflicti224K → N in AAA67917 (PubMed:7959722).Curated1
Sequence conflicti398C → S in AAA67917 (PubMed:7959722).Curated1
Sequence conflicti450K → R in AAA67917 (PubMed:7959722).Curated1
Sequence conflicti528S → R in AAA67917 (PubMed:7959722).Curated1
Sequence conflicti621C → S in AAA62275 (PubMed:7919657).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03487 mRNA. Translation: AAA62275.1.
U04055 mRNA. Translation: AAA67916.1.
U04056 Genomic DNA. Translation: AAA67917.1.
L35933 mRNA. Translation: AAA39875.1.
AK143841 mRNA. Translation: BAE25564.1.
AL844548 Genomic DNA. Translation: CAM19015.1.
CCDSiCCDS16631.1.
PIRiA54741.
RefSeqiNP_038541.1. NM_013513.3.
UniGeneiMm.240051.

Genome annotation databases

EnsembliENSMUST00000102490; ENSMUSP00000099548; ENSMUSG00000023216.
GeneIDi13828.
KEGGimmu:13828.
UCSCiuc008lxi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03487 mRNA. Translation: AAA62275.1.
U04055 mRNA. Translation: AAA67916.1.
U04056 Genomic DNA. Translation: AAA67917.1.
L35933 mRNA. Translation: AAA39875.1.
AK143841 mRNA. Translation: BAE25564.1.
AL844548 Genomic DNA. Translation: CAM19015.1.
CCDSiCCDS16631.1.
PIRiA54741.
RefSeqiNP_038541.1. NM_013513.3.
UniGeneiMm.240051.

3D structure databases

ProteinModelPortaliP49222.
SMRiP49222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49222. 2 interactors.
MINTiMINT-4094485.
STRINGi10090.ENSMUSP00000099548.

PTM databases

iPTMnetiP49222.
PhosphoSitePlusiP49222.

Proteomic databases

MaxQBiP49222.
PaxDbiP49222.
PeptideAtlasiP49222.
PRIDEiP49222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102490; ENSMUSP00000099548; ENSMUSG00000023216.
GeneIDi13828.
KEGGimmu:13828.
UCSCiuc008lxi.1. mouse.

Organism-specific databases

CTDi2038.
MGIiMGI:95402. Epb42.

Phylogenomic databases

eggNOGiENOG410IH88. Eukaryota.
ENOG410YC7E. LUCA.
GeneTreeiENSGT00760000119108.
HOGENOMiHOG000231695.
HOVERGENiHBG106048.
InParanoidiP49222.
OMAiSCFAQED.
OrthoDBiEOG091G030K.
TreeFamiTF324278.

Miscellaneous databases

PROiP49222.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023216.
CleanExiMM_EPB4.2.
ExpressionAtlasiP49222. baseline and differential.
GenevisibleiP49222. MM.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPB42_MOUSE
AccessioniPrimary (citable) accession number: P49222
Secondary accession number(s): Q3UP33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The substitution of an Ala for a Cys in the active site may be responsible for the lack of transglutaminase activity of band 4.2.

Caution

Was originally thought to be pallidin.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.