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Protein

Protein-glutamine gamma-glutamyltransferase 4

Gene

TGM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei268 – 2681PROSITE-ProRule annotation
Active sitei327 – 3271PROSITE-ProRule annotation
Active sitei350 – 3501PROSITE-ProRule annotation
Metal bindingi390 – 3901CalciumBy similarity
Metal bindingi392 – 3921CalciumBy similarity
Metal bindingi442 – 4421CalciumBy similarity
Metal bindingi447 – 4471CalciumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mating plug formation Source: Ensembl
  2. peptide cross-linking Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase 4 (EC:2.3.2.13)
Alternative name(s):
Fibrinoligase
Prostate transglutaminase
Prostate-specific transglutaminase
Transglutaminase P
Short name:
TG(P)
Short name:
TGP
Short name:
TGase P
Transglutaminase-4
Short name:
TGase-4
Gene namesi
Name:TGM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:11780. TGM4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. extracellular matrix Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProtKB
  4. Golgi apparatus Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36493.

Chemistry

DrugBankiDB00130. L-Glutamine.

Polymorphism and mutation databases

BioMutaiTGM4.
DMDMi3915892.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 684684Protein-glutamine gamma-glutamyltransferase 4PRO_0000213711Add
BLAST

Proteomic databases

PaxDbiP49221.
PRIDEiP49221.

PTM databases

PhosphoSiteiP49221.

Expressioni

Tissue specificityi

Prostate.

Gene expression databases

BgeeiP49221.
CleanExiHS_TGM4.
ExpressionAtlasiP49221. baseline and differential.
GenevestigatoriP49221.

Organism-specific databases

HPAiCAB033571.
HPA032072.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi112905. 5 interactions.
IntActiP49221. 2 interactions.
STRINGi9606.ENSP00000296125.

Structurei

3D structure databases

ProteinModelPortaliP49221.
SMRiP49221. Positions 8-676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG80379.
GeneTreeiENSGT00760000119108.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiP49221.
KOiK05621.
OMAiHVWTDAW.
OrthoDBiEOG7WT40M.
PhylomeDBiP49221.
TreeFamiTF324278.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49221-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDASKELQV LHIDFLNQDN AVSHHTWEFQ TSSPVFRRGQ VFHLRLVLNQ
60 70 80 90 100
PLQSYHQLKL EFSTGPNPSI AKHTLVVLDP RTPSDHYNWQ ATLQNESGKE
110 120 130 140 150
VTVAVTSSPN AILGKYQLNV KTGNHILKSE ENILYLLFNP WCKEDMVFMP
160 170 180 190 200
DEDERKEYIL NDTGCHYVGA ARSIKCKPWN FGQFEKNVLD CCISLLTESS
210 220 230 240 250
LKPTDRRDPV LVCRAMCAMM SFEKGQGVLI GNWTGDYEGG TAPYKWTGSA
260 270 280 290 300
PILQQYYNTK QAVCFGQCWV FAGILTTVLR ALGIPARSVT GFDSAHDTER
310 320 330 340 350
NLTVDTYVNE NGEKITSMTH DSVWNFHVWT DAWMKRPDLP KGYDGWQAVD
360 370 380 390 400
ATPQERSQGV FCCGPSPLTA IRKGDIFIVY DTRFVFSEVN GDRLIWLVKM
410 420 430 440 450
VNGQEELHVI SMETTSIGKN ISTKAVGQDR RRDITYEYKY PEGSSEERQV
460 470 480 490 500
MDHAFLLLSS EREHRRPVKE NFLHMSVQSD DVLLGNSVNF TVILKRKTAA
510 520 530 540 550
LQNVNILGSF ELQLYTGKKM AKLCDLNKTS QIQGQVSEVT LTLDSKTYIN
560 570 580 590 600
SLAILDDEPV IRGFIIAEIV ESKEIMASEV FTSFQYPEFS IELPNTGRIG
610 620 630 640 650
QLLVCNCIFK NTLAIPLTDV KFSLESLGIS SLQTSDHGTV QPGETIQSQI
660 670 680
KCTPIKTGPK KFIVKLSSKQ VKEINAQKIV LITK
Length:684
Mass (Da):77,145
Last modified:December 15, 1998 - v2
Checksum:i64A3E754EA46BFEC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531P → S in AAH07003 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti100 – 1001E → D.
Corresponds to variant rs2271087 [ dbSNP | Ensembl ].
VAR_052555
Natural varianti244 – 2441Y → H.
Corresponds to variant rs9818345 [ dbSNP | Ensembl ].
VAR_052556
Natural varianti249 – 2491S → T.
Corresponds to variant rs937838 [ dbSNP | Ensembl ].
VAR_052557
Natural varianti313 – 3131E → K.1 Publication
Corresponds to variant rs1995641 [ dbSNP | Ensembl ].
VAR_052558
Natural varianti372 – 3721R → C.
Corresponds to variant rs3749195 [ dbSNP | Ensembl ].
VAR_052559
Natural varianti372 – 3721R → H.
Corresponds to variant rs13326552 [ dbSNP | Ensembl ].
VAR_052560
Natural varianti376 – 3761I → V.
Corresponds to variant rs17077022 [ dbSNP | Ensembl ].
VAR_052561
Natural varianti409 – 4091V → I.
Corresponds to variant rs9876921 [ dbSNP | Ensembl ].
VAR_052562
Natural varianti437 – 4371E → Q.
Corresponds to variant rs1395388 [ dbSNP | Ensembl ].
VAR_052563

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34840 mRNA. Translation: AAB95430.1.
U31905 mRNA. Translation: AAC50516.1.
BC007003 mRNA. Translation: AAH07003.1.
CCDSiCCDS2723.1.
PIRiS71105.
RefSeqiNP_003232.2. NM_003241.3.
UniGeneiHs.438265.

Genome annotation databases

EnsembliENST00000296125; ENSP00000296125; ENSG00000163810.
GeneIDi7047.
KEGGihsa:7047.
UCSCiuc003coc.4. human.

Polymorphism and mutation databases

BioMutaiTGM4.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34840 mRNA. Translation: AAB95430.1.
U31905 mRNA. Translation: AAC50516.1.
BC007003 mRNA. Translation: AAH07003.1.
CCDSiCCDS2723.1.
PIRiS71105.
RefSeqiNP_003232.2. NM_003241.3.
UniGeneiHs.438265.

3D structure databases

ProteinModelPortaliP49221.
SMRiP49221. Positions 8-676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112905. 5 interactions.
IntActiP49221. 2 interactions.
STRINGi9606.ENSP00000296125.

Chemistry

DrugBankiDB00130. L-Glutamine.

PTM databases

PhosphoSiteiP49221.

Polymorphism and mutation databases

BioMutaiTGM4.
DMDMi3915892.

Proteomic databases

PaxDbiP49221.
PRIDEiP49221.

Protocols and materials databases

DNASUi7047.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296125; ENSP00000296125; ENSG00000163810.
GeneIDi7047.
KEGGihsa:7047.
UCSCiuc003coc.4. human.

Organism-specific databases

CTDi7047.
GeneCardsiGC03P044916.
HGNCiHGNC:11780. TGM4.
HPAiCAB033571.
HPA032072.
MIMi600585. gene.
neXtProtiNX_P49221.
PharmGKBiPA36493.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG80379.
GeneTreeiENSGT00760000119108.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiP49221.
KOiK05621.
OMAiHVWTDAW.
OrthoDBiEOG7WT40M.
PhylomeDBiP49221.
TreeFamiTF324278.

Miscellaneous databases

GeneWikiiTGM4.
GenomeRNAii7047.
NextBioi27537.
PROiP49221.
SOURCEiSearch...

Gene expression databases

BgeeiP49221.
CleanExiHS_TGM4.
ExpressionAtlasiP49221. baseline and differential.
GenevestigatoriP49221.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel transglutaminase cDNA from a human prostate cDNA library."
    Grant F.J., Taylor D.A., Sheppard P.O., Mathewes S.L., Lint W., Vanaja E., Bishop P.D., O'Hara P.J.
    Biochem. Biophys. Res. Commun. 203:1117-1123(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Prostate.
  2. Grant F.J.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Tissue specific and androgen-regulated expression of human prostate-specific transglutaminase."
    Dubbink H.J., Verkaik N.S., Faber P.W., Trapman J., Schroeder F.H., Romijn J.C.
    Biochem. J. 315:901-908(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, VARIANT LYS-313.
    Tissue: Prostate.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiTGM4_HUMAN
AccessioniPrimary (citable) accession number: P49221
Secondary accession number(s): Q16707, Q96QN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 15, 1998
Last modified: April 29, 2015
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.