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P49192 (CART_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cocaine- and amphetamine-regulated transcript protein

Cleaved into the following 3 chains:

  1. CART(1-52)
  2. CART(55-102)
  3. CART(62-102)
Gene names
Name:Cartpt
Synonyms:Cart
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length129 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus By similarity.

Subcellular location

Secreted Potential.

Tissue specificity

Neuroendocrine tissues. Predominantly expressed in the hypothalamus, pituitary, and longitudinal muscle-myenteric plexus. Abundant expression is also seen in the midbrain/thalamus and eye. A lower level expression is seen in the other brain regions and adrenal.

Induction

By cocaine and amphetamine.

Sequence similarities

Belongs to the CART family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionNeuropeptide
Neurotransmitter
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 15908120. Source: HGNC

activation of MAPKK activity

Inferred from direct assay PubMed 15908120. Source: HGNC

adult feeding behavior

Inferred from direct assay PubMed 9590691. Source: HGNC

cellular glucose homeostasis

Inferred from sequence or structural similarity. Source: HGNC

cellular response to starvation

Inferred from direct assay PubMed 15680948PubMed 9590691. Source: HGNC

circadian regulation of gene expression

Inferred from direct assay PubMed 15680948. Source: HGNC

gastric acid secretion

Traceable author statement PubMed 10919272. Source: RGD

negative regulation of appetite

Inferred from direct assay PubMed 9590691. Source: HGNC

negative regulation of bone resorption

Inferred from electronic annotation. Source: Ensembl

negative regulation of glucagon secretion

Inferred from direct assay PubMed 16443761. Source: RGD

neuropeptide signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of blood pressure

Inferred from sequence or structural similarity. Source: HGNC

positive regulation of epinephrine secretion

Inferred from sequence or structural similarity. Source: HGNC

positive regulation of transmission of nerve impulse

Inferred from sequence or structural similarity. Source: HGNC

regulation of insulin secretion

Inferred from direct assay PubMed 16443761. Source: RGD

somatostatin secretion

Inferred from direct assay PubMed 16443761. Source: RGD

synaptic transmission

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from direct assay PubMed 9590691. Source: HGNC

secretory granule

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 17064765. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P49192-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P49192-2)

The sequence of this isoform differs from the canonical sequence as follows:
     54-66: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 129102Cocaine- and amphetamine-regulated transcript protein
PRO_0000004442
Peptide28 – 7952CART(1-52)
PRO_0000004443
Peptide82 – 12948CART(55-102)
PRO_0000004444
Peptide89 – 12941CART(62-102) Potential
PRO_0000004445

Amino acid modifications

Disulfide bond95 ↔ 113 Ref.2
Disulfide bond101 ↔ 121 Ref.2
Disulfide bond115 ↔ 128 Ref.2

Natural variations

Alternative sequence54 – 6613Missing in isoform Short.
VSP_000794

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 0FDE28B705BB2728

FASTA12914,140
        10         20         30         40         50         60 
MESSRLRLLP VLGAALLLLL PLLGAGAQED AELQPRALDI YSAVDDASHE KELPRRQLRA 

        70         80         90        100        110        120 
PGAVLQIEAL QEVLKKLKSK RIPIYEKKYG QVPMCDAGEQ CAVRKGARIG KLCDCPRGTS 


CNSFLLKCL 

« Hide

Isoform Short [UniParc].

Checksum: 5545F930A182BD61
Show »

FASTA11612,696

References

[1]"PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamine."
Douglass J.O., McKinzie A.A., Couceyro P.
J. Neurosci. 15:2471-2481(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Purification and characterisation of a new hypothalamic satiety peptide, cocaine and amphetamine regulated transcript (CART), produced in yeast."
Thim L., Nielsen P.F., Judge M.E., Andersen A.S., Diers I., Egel-Mitani M., Hastrup S.
FEBS Lett. 428:263-268(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 81-97, IDENTIFICATION BY MASS SPECTROMETRY, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10071 mRNA. Translation: AAA87897.1.
RefSeqNP_058806.1. NM_017110.1. [P49192-1]
XP_006231905.1. XM_006231843.1. [P49192-2]
UniGeneRn.89164.

3D structure databases

ProteinModelPortalP49192.
SMRP49192. Positions 89-129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023869.

PTM databases

PhosphoSiteP49192.

Proteomic databases

PRIDEP49192.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023869; ENSRNOP00000023869; ENSRNOG00000017712. [P49192-1]
GeneID29131.
KEGGrno:29131.
UCSCRGD:2272. rat. [P49192-1]

Organism-specific databases

CTD9607.
RGD2272. Cartpt.

Phylogenomic databases

eggNOGNOG47455.
GeneTreeENSGT00390000018319.
HOGENOMHOG000111306.
HOVERGENHBG018929.
InParanoidP49192.
OMASHEKELP.
OrthoDBEOG7JDR0T.
PhylomeDBP49192.
TreeFamTF332948.

Gene expression databases

ArrayExpressP49192.
GenevestigatorP49192.

Family and domain databases

Gene3D4.10.40.30. 1 hit.
InterProIPR009106. CART.
[Graphical view]
PANTHERPTHR16655. PTHR16655. 1 hit.
PfamPF06373. CART. 1 hit.
[Graphical view]
SUPFAMSSF64546. SSF64546. 1 hit.
ProtoNetSearch...

Other

NextBio608079.
PROP49192.

Entry information

Entry nameCART_RAT
AccessionPrimary (citable) accession number: P49192
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families