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Protein

Cocaine- and amphetamine-regulated transcript protein

Gene

Cartpt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus.By similarity

GO - Biological processi

  1. activation of MAPKK activity Source: HGNC
  2. adult feeding behavior Source: HGNC
  3. cellular glucose homeostasis Source: HGNC
  4. cellular response to starvation Source: HGNC
  5. circadian regulation of gene expression Source: HGNC
  6. gastric acid secretion Source: RGD
  7. G-protein coupled receptor signaling pathway Source: HGNC
  8. negative regulation of appetite Source: HGNC
  9. negative regulation of bone resorption Source: Ensembl
  10. negative regulation of glucagon secretion Source: RGD
  11. neuropeptide signaling pathway Source: UniProtKB-KW
  12. positive regulation of blood pressure Source: HGNC
  13. positive regulation of epinephrine secretion Source: HGNC
  14. positive regulation of transmission of nerve impulse Source: HGNC
  15. regulation of insulin secretion Source: RGD
  16. somatostatin secretion Source: RGD
  17. synaptic transmission Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide, Neurotransmitter

Names & Taxonomyi

Protein namesi
Recommended name:
Cocaine- and amphetamine-regulated transcript protein
Cleaved into the following 3 chains:
Gene namesi
Name:Cartpt
Synonyms:Cart
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2272. Cartpt.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular space Source: HGNC
  2. secretory granule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 129102Cocaine- and amphetamine-regulated transcript proteinPRO_0000004442Add
BLAST
Peptidei28 – 7952CART(1-52)PRO_0000004443Add
BLAST
Peptidei82 – 12948CART(55-102)PRO_0000004444Add
BLAST
Peptidei89 – 12941CART(62-102)Sequence AnalysisPRO_0000004445Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi95 ↔ 1131 Publication
Disulfide bondi101 ↔ 1211 Publication
Disulfide bondi115 ↔ 1281 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiP49192.

PTM databases

PhosphoSiteiP49192.

Expressioni

Tissue specificityi

Neuroendocrine tissues. Predominantly expressed in the hypothalamus, pituitary, and longitudinal muscle-myenteric plexus. Abundant expression is also seen in the midbrain/thalamus and eye. A lower level expression is seen in the other brain regions and adrenal.

Inductioni

By cocaine and amphetamine.

Gene expression databases

ExpressionAtlasiP49192. baseline and differential.
GenevestigatoriP49192.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023869.

Structurei

3D structure databases

SMRiP49192. Positions 89-129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CART family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG47455.
GeneTreeiENSGT00390000018319.
HOGENOMiHOG000111306.
HOVERGENiHBG018929.
InParanoidiP49192.
OMAiSHEKELP.
OrthoDBiEOG7JDR0T.
PhylomeDBiP49192.
TreeFamiTF332948.

Family and domain databases

Gene3Di4.10.40.30. 1 hit.
InterProiIPR009106. CART.
[Graphical view]
PANTHERiPTHR16655. PTHR16655. 1 hit.
PfamiPF06373. CART. 1 hit.
[Graphical view]
SUPFAMiSSF64546. SSF64546. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P49192-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESSRLRLLP VLGAALLLLL PLLGAGAQED AELQPRALDI YSAVDDASHE
60 70 80 90 100
KELPRRQLRA PGAVLQIEAL QEVLKKLKSK RIPIYEKKYG QVPMCDAGEQ
110 120
CAVRKGARIG KLCDCPRGTS CNSFLLKCL
Length:129
Mass (Da):14,140
Last modified:January 31, 1996 - v1
Checksum:i0FDE28B705BB2728
GO
Isoform Short (identifier: P49192-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-66: Missing.

Show »
Length:116
Mass (Da):12,696
Checksum:i5545F930A182BD61
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 6613Missing in isoform Short. 1 PublicationVSP_000794Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10071 mRNA. Translation: AAA87897.1.
RefSeqiNP_058806.1. NM_017110.1. [P49192-1]
XP_006231905.1. XM_006231843.2. [P49192-2]
UniGeneiRn.89164.

Genome annotation databases

EnsembliENSRNOT00000023869; ENSRNOP00000023869; ENSRNOG00000017712. [P49192-1]
GeneIDi29131.
KEGGirno:29131.
UCSCiRGD:2272. rat. [P49192-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10071 mRNA. Translation: AAA87897.1.
RefSeqiNP_058806.1. NM_017110.1. [P49192-1]
XP_006231905.1. XM_006231843.2. [P49192-2]
UniGeneiRn.89164.

3D structure databases

SMRiP49192. Positions 89-129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023869.

PTM databases

PhosphoSiteiP49192.

Proteomic databases

PRIDEiP49192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023869; ENSRNOP00000023869; ENSRNOG00000017712. [P49192-1]
GeneIDi29131.
KEGGirno:29131.
UCSCiRGD:2272. rat. [P49192-1]

Organism-specific databases

CTDi9607.
RGDi2272. Cartpt.

Phylogenomic databases

eggNOGiNOG47455.
GeneTreeiENSGT00390000018319.
HOGENOMiHOG000111306.
HOVERGENiHBG018929.
InParanoidiP49192.
OMAiSHEKELP.
OrthoDBiEOG7JDR0T.
PhylomeDBiP49192.
TreeFamiTF332948.

Miscellaneous databases

NextBioi608079.
PROiP49192.

Gene expression databases

ExpressionAtlasiP49192. baseline and differential.
GenevestigatoriP49192.

Family and domain databases

Gene3Di4.10.40.30. 1 hit.
InterProiIPR009106. CART.
[Graphical view]
PANTHERiPTHR16655. PTHR16655. 1 hit.
PfamiPF06373. CART. 1 hit.
[Graphical view]
SUPFAMiSSF64546. SSF64546. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamine."
    Douglass J.O., McKinzie A.A., Couceyro P.
    J. Neurosci. 15:2471-2481(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Purification and characterisation of a new hypothalamic satiety peptide, cocaine and amphetamine regulated transcript (CART), produced in yeast."
    Thim L., Nielsen P.F., Judge M.E., Andersen A.S., Diers I., Egel-Mitani M., Hastrup S.
    FEBS Lett. 428:263-268(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 81-97, IDENTIFICATION BY MASS SPECTROMETRY, DISULFIDE BONDS.

Entry informationi

Entry nameiCART_RAT
AccessioniPrimary (citable) accession number: P49192
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: January 31, 1996
Last modified: March 31, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.