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Reviewed, UniProtKB/Swiss-Prot P49186 (MK09_RAT)

Last modified February 9, 2010. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Mitogen-activated protein kinase 9
      Short name=MAP kinase 9
      Short name=MAPK 9
    EC=2.7.11.24
Alternative name(s):
    Stress-activated protein kinase JNK2
    c-Jun N-terminal kinase 2
    SAPK-alpha
    p54-alpha
Gene names
Name: Mapk9
Synonyms: Jnk2, Prkm9
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1 By similarity.

Subunit structure

Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with NFATC4 By similarity. Interacts with MECOM.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme By similarity. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

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Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processJUN phosphorylation

Inferred from mutant phenotype. Source: RGD

cellular response to UV

Inferred from mutant phenotype. Source: RGD

central nervous system development

Inferred from expression pattern. Source: RGD

neuron projection development

Inferred from mutant phenotype. Source: RGD

positive regulation of apoptosis

Inferred from mutant phenotype. Source: RGD

positive regulation of caspase activity

Inferred from mutant phenotype. Source: RGD

positive regulation of cell morphogenesis involved in differentiation

Inferred from mutant phenotype. Source: RGD

positive regulation of chemokine production

Inferred from mutant phenotype. Source: RGD

positive regulation of nitric oxide biosynthetic process

Inferred from mutant phenotype. Source: RGD

positive regulation of nitric-oxide synthase 2 biosynthetic process

Inferred from mutant phenotype. Source: RGD

positive regulation of prostaglandin biosynthetic process

Inferred from mutant phenotype. Source: RGD

positive regulation of prostaglandin secretion

Inferred from mutant phenotype. Source: RGD

positive regulation of protein amino acid phosphorylation

Inferred from mutant phenotype. Source: RGD

positive regulation of transcription

Inferred from mutant phenotype. Source: RGD

protein targeting to mitochondrion

Inferred from expression pattern. Source: RGD

regulation of JNK cascade

Inferred from mutant phenotype. Source: RGD

regulation of protein ubiquitination

Inferred from mutant phenotype. Source: RGD

release of cytochrome c from mitochondria

Inferred from mutant phenotype. Source: RGD

response to amine stimulus

Inferred from expression pattern. Source: RGD

response to cytokine stimulus

Inferred from expression pattern. Source: RGD

response to drug

Inferred from mutant phenotype. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern. Source: RGD

response to mechanical stimulus

Inferred from expression pattern. Source: RGD

response to protein stimulus

Inferred from mutant phenotype. Source: RGD

response to toxin

Inferred from expression pattern. Source: RGD

   Cellular componentcytosolic part

Inferred from direct assay. Source: MGI

mitochondrion

Inferred from direct assay. Source: RGD

nucleus

Inferred from direct assay. Source: RGD

   Molecular functionATP binding

Inferred from direct assay. Source: RGD

JUN kinase activity

Inferred from direct assay. Source: RGD

caspase activator activity

Inferred from mutant phenotype. Source: RGD

mitogen-activated protein kinase kinase kinase binding

Inferred from physical interaction. Source: RGD

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha-2 (identifier: P49186-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha-1 (identifier: P49186-2)

The sequence of this isoform differs from the canonical sequence as follows:
     216-230: GELVKGCVIFQGTDH → AEMVLHKSCSPGRDY

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Mitogen-activated protein kinase 9
PRO_0000186275

Regions

Domain26 – 321296Protein kinase
Nucleotide binding32 – 409ATP By similarity
Motif183 – 1853TXY

Sites

Active site1511Proton acceptor By similarity
Binding site551ATP By similarity

Amino acid modifications

Modified residue1831Phosphothreonine By similarity
Modified residue1851Phosphotyrosine By similarity
Modified residue4031Phosphothreonine By similarity

Natural variations

Alternative sequence216 – 23015GELVK…QGTDH → AEMVLHKSCSPGRDY in isoform Alpha-1.
VSP_004838

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Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha-2 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: EE549B9F4F12F421

FASTA42348,017
        10         20         30         40         50         60 
MSDSKSDGQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP 

        70         80         90        100        110        120 
FQNQTHAKRA YRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH 

       130        140        150        160        170        180 
MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF 

       190        200        210        220        230        240 
MMTPYVVTRY YRAPEVILGM GYKENVDIWS VGCIMGELVK GCVIFQGTDH IDQWNKVIEQ 

       250        260        270        280        290        300 
LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIF PSESERDKIK TSQARDLLSK 

       310        320        330        340        350        360 
MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIE EWKELIYKEV 

       370        380        390        400        410        420 
MDWEERSKNG VKDQPSDAAV SSKATPSQSS SINDISSMST EHTLASDTDS SLDASTGPLE 


GCR

« Hide

Isoform Alpha-1.

Checksum: 6419E1052BDC0292
Show »

FASTA42348,107

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References

« Hide 'large scale' references
[1]"The stress-activated protein kinase subfamily of c-Jun kinases."
Kyriakis J.M., Banerjee P., Nikolakaki E., Dai T., Rubie E.A., Ahmad M.F., Avruch J., Woodgett J.R.
Nature 369:156-160(1994) [PubMed: 8177321] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-1 AND ALPHA-2), PARTIAL PROTEIN SEQUENCE.
Tissue: Brain.
[2]"The evi-1 oncoprotein inhibits c-Jun N-terminal kinase and prevents stress-induced cell death."
Kurokawa M., Mitani K., Yamagata T., Takahashi T., Izutsu K., Ogawa S., Moriguchi T., Nishida E., Yazaki Y., Hirai H.
EMBO J. 19:2958-2968(2000) [PubMed: 10856240] [Abstract]
Cited for: INTERACTION WITH MECOM.
[3]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-183 AND TYR-185, MASS SPECTROMETRY.
Tissue: Kidney.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L27112 mRNA. Translation: AAA42109.1.
L27111 mRNA. Translation: AAA42108.1.
IPIIPI00191806.
IPI00231634.
PIRS43968.
RefSeqNP_059018.1.
UniGeneRn.9910

3D structure databases

SMRP49186. Positions 7-362.
ModBaseSearch...

Protein-protein interaction databases

STRINGP49186.

PTM databases

PhosphoSiteP49186.

Genome annotation databases

EnsemblENSRNOT00000003987; ENSRNOP00000003987; ENSRNOG00000002823; Rattus norvegicus. [Genome view]
GeneID50658.
KEGGrno:50658.
UCSCBC061870. rat.
L27111. rat.

Organism-specific databases

CTD50658.
RGD628847. Mapk9.

Phylogenomic databases

eggNOGroNOG08548.
HOVERGENP49186.
InParanoidP49186.
OMANGVVKDQ.
OrthoDBEOG91C9FM.
PhylomeDBP49186.

Enzyme and pathway databases

BRENDA2.7.11.24. 248.

Gene expression databases

ArrayExpressP49186.
GenevestigatorP49186.
GermOnlineENSRNOG00000002823. Rattus norvegicus.

Family and domain databases

InterProIPR008351. JNK_MAPK.
IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_cat_dom.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio610470.

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Entry information

Entry nameMK09_RAT
AccessionPrimary (citable) accession number: P49186
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 9, 2010
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents