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Protein

Deoxyribonuclease-1

Gene

Dnase1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Ca2+, Mg2+Note: Divalent metal cations. Prefers Ca(2+) or Mg2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei100 – 1001By similarity
Active sitei156 – 1561By similarity

GO - Molecular functioni

  • deoxyribonuclease I activity Source: CACAO
  • endodeoxyribonuclease activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Actin-binding, Calcium

Enzyme and pathway databases

BRENDAi3.1.21.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-1 (EC:3.1.21.1)
Alternative name(s):
Deoxyribonuclease I
Short name:
DNase I
Gene namesi
Name:Dnase1
Synonyms:Dnl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:103157. Dnase1.

Subcellular locationi

  • Secreted By similarity
  • Nucleus envelope By similarity

  • Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 284262Deoxyribonuclease-1PRO_0000007278Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi123 ↔ 126By similarity
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi195 ↔ 231Essential for enzymatic activityBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP49183.
PaxDbiP49183.
PRIDEiP49183.

PTM databases

PhosphoSiteiP49183.

Expressioni

Tissue specificityi

In striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.

Gene expression databases

BgeeiP49183.
CleanExiMM_DNASE1.
ExpressionAtlasiP49183. baseline and differential.
GenevisibleiP49183. MM.

Interactioni

Protein-protein interaction databases

IntActiP49183. 1 interaction.
STRINGi10090.ENSMUSP00000006136.

Structurei

3D structure databases

ProteinModelPortaliP49183.
SMRiP49183. Positions 23-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46375.
HOGENOMiHOG000059570.
HOVERGENiHBG051368.
InParanoidiP49183.
KOiK11994.
OMAiTSTHCAY.
PhylomeDBiP49183.
TreeFamiTF329541.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR11371. PTHR11371. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYTGLMGTL LTLVNLLQLA GTLRIAAFNI RTFGETKMSN ATLSVYFVKI
60 70 80 90 100
LSRYDIAVIQ EVRDSHLVAV GKLLDELNRD KPDTYRYVVS EPLGRKSYKE
110 120 130 140 150
QYLFVYRPDQ VSILDSYQYD DGCEPCGNDT FSREPAIVKF FSPYTEVQEF
160 170 180 190 200
AIVPLHAAPT EAVSEIDALY DVYLDVWQKW GLEDIMFMGD FNAGCSYVTS
210 220 230 240 250
SQWSSIRLRT SPIFQWLIPD SADTTVTSTH CAYDRIVVAG ALLQAAVVPN
260 270 280
SAVPFDFQAE YGLSNQLAEA ISDHYPVEVT LRKI
Length:284
Mass (Da):32,027
Last modified:July 15, 1999 - v2
Checksum:i8682E20515EEB510
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti239 – 2402AG → VR in AAA03710 (PubMed:7857306).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00478 mRNA. Translation: AAA03710.1.
D83038 mRNA. Translation: BAA28622.1.
CCDSiCCDS27913.1.
PIRiJC2526.
RefSeqiNP_034191.3. NM_010061.5.
XP_006521837.1. XM_006521774.2.
XP_006521838.1. XM_006521775.2.
XP_006521839.1. XM_006521776.2.
UniGeneiMm.440565.

Genome annotation databases

EnsembliENSMUST00000006136; ENSMUSP00000006136; ENSMUSG00000005980.
ENSMUST00000120009; ENSMUSP00000113119; ENSMUSG00000005980.
GeneIDi13419.
KEGGimmu:13419.
UCSCiuc007xzh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00478 mRNA. Translation: AAA03710.1.
D83038 mRNA. Translation: BAA28622.1.
CCDSiCCDS27913.1.
PIRiJC2526.
RefSeqiNP_034191.3. NM_010061.5.
XP_006521837.1. XM_006521774.2.
XP_006521838.1. XM_006521775.2.
XP_006521839.1. XM_006521776.2.
UniGeneiMm.440565.

3D structure databases

ProteinModelPortaliP49183.
SMRiP49183. Positions 23-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP49183. 1 interaction.
STRINGi10090.ENSMUSP00000006136.

PTM databases

PhosphoSiteiP49183.

Proteomic databases

MaxQBiP49183.
PaxDbiP49183.
PRIDEiP49183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006136; ENSMUSP00000006136; ENSMUSG00000005980.
ENSMUST00000120009; ENSMUSP00000113119; ENSMUSG00000005980.
GeneIDi13419.
KEGGimmu:13419.
UCSCiuc007xzh.1. mouse.

Organism-specific databases

CTDi1773.
MGIiMGI:103157. Dnase1.

Phylogenomic databases

eggNOGiNOG46375.
HOGENOMiHOG000059570.
HOVERGENiHBG051368.
InParanoidiP49183.
KOiK11994.
OMAiTSTHCAY.
PhylomeDBiP49183.
TreeFamiTF329541.

Enzyme and pathway databases

BRENDAi3.1.21.1. 3474.

Miscellaneous databases

ChiTaRSiDnase1. mouse.
NextBioi283827.
PROiP49183.
SOURCEiSearch...

Gene expression databases

BgeeiP49183.
CleanExiMM_DNASE1.
ExpressionAtlasiP49183. baseline and differential.
GenevisibleiP49183. MM.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR11371. PTHR11371. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic organisation and expression of mouse deoxyribonuclease I."
    Peitsch M.C., Irmler M., French L.E., Tschopp J.
    Biochem. Biophys. Res. Commun. 207:62-68(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Heart.
  2. "Mouse deoxyribonuclease I (DNase I): biochemical and immunological characterization, cDNA structure and tissue distribution."
    Takeshita H., Yasuda T., Nakajima T., Hosomi O., Nakashima Y., Kishi K.
    Biochem. Mol. Biol. Int. 42:65-75(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Kidney and Parotid gland.

Entry informationi

Entry nameiDNAS1_MOUSE
AccessioniPrimary (citable) accession number: P49183
Secondary accession number(s): O70532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: June 24, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.