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P49183 (DNAS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyribonuclease-1

EC=3.1.21.1
Alternative name(s):
Deoxyribonuclease I
Short name=DNase I
Gene names
Name:Dnase1
Synonyms:Dnl1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization By similarity.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

Cofactor

Divalent cations. Prefers calcium or magnesium.

Subcellular location

Secreted By similarity. Nucleus envelope By similarity. Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope By similarity.

Tissue specificity

In striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.

Sequence similarities

Belongs to the DNase I family.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentNucleus
Secreted
   DomainSignal
   LigandActin-binding
Calcium
   Molecular functionEndonuclease
Hydrolase
Nuclease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear envelope

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondeoxyribonuclease I activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 284262Deoxyribonuclease-1
PRO_0000007278

Sites

Active site1001 By similarity
Active site1561 By similarity

Amino acid modifications

Glycosylation401N-linked (GlcNAc...) Potential
Glycosylation1281N-linked (GlcNAc...) Potential
Disulfide bond123 ↔ 126 By similarity
Disulfide bond195 ↔ 231Essential for enzymatic activity By similarity

Experimental info

Sequence conflict239 – 2402AG → VR in AAA03710. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P49183 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 8682E20515EEB510

FASTA28432,027
        10         20         30         40         50         60 
MRYTGLMGTL LTLVNLLQLA GTLRIAAFNI RTFGETKMSN ATLSVYFVKI LSRYDIAVIQ 

        70         80         90        100        110        120 
EVRDSHLVAV GKLLDELNRD KPDTYRYVVS EPLGRKSYKE QYLFVYRPDQ VSILDSYQYD 

       130        140        150        160        170        180 
DGCEPCGNDT FSREPAIVKF FSPYTEVQEF AIVPLHAAPT EAVSEIDALY DVYLDVWQKW 

       190        200        210        220        230        240 
GLEDIMFMGD FNAGCSYVTS SQWSSIRLRT SPIFQWLIPD SADTTVTSTH CAYDRIVVAG 

       250        260        270        280 
ALLQAAVVPN SAVPFDFQAE YGLSNQLAEA ISDHYPVEVT LRKI 

« Hide

References

[1]"Genomic organisation and expression of mouse deoxyribonuclease I."
Peitsch M.C., Irmler M., French L.E., Tschopp J.
Biochem. Biophys. Res. Commun. 207:62-68(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Heart.
[2]"Mouse deoxyribonuclease I (DNase I): biochemical and immunological characterization, cDNA structure and tissue distribution."
Takeshita H., Yasuda T., Nakajima T., Hosomi O., Nakashima Y., Kishi K.
Biochem. Mol. Biol. Int. 42:65-75(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Kidney and Parotid gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00478 mRNA. Translation: AAA03710.1.
D83038 mRNA. Translation: BAA28622.1.
CCDSCCDS27913.1.
PIRJC2526.
RefSeqNP_034191.3. NM_010061.5.
XP_006521837.1. XM_006521774.1.
XP_006521838.1. XM_006521775.1.
XP_006521839.1. XM_006521776.1.
UniGeneMm.440565.

3D structure databases

ProteinModelPortalP49183.
SMRP49183. Positions 23-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP49183. 1 interaction.

PTM databases

PhosphoSiteP49183.

Proteomic databases

MaxQBP49183.
PaxDbP49183.
PRIDEP49183.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006136; ENSMUSP00000006136; ENSMUSG00000005980.
ENSMUST00000120009; ENSMUSP00000113119; ENSMUSG00000005980.
GeneID13419.
KEGGmmu:13419.
UCSCuc007xzh.1. mouse.

Organism-specific databases

CTD1773.
MGIMGI:103157. Dnase1.

Phylogenomic databases

eggNOGNOG46375.
HOGENOMHOG000059570.
HOVERGENHBG051368.
InParanoidP49183.
KOK11994.
OMATSTHCAY.
PhylomeDBP49183.
TreeFamTF329541.

Enzyme and pathway databases

BRENDA3.1.21.1. 3474.

Gene expression databases

BgeeP49183.
CleanExMM_DNASE1.
GenevestigatorP49183.

Family and domain databases

Gene3D3.60.10.10. 1 hit.
InterProIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERPTHR11371. PTHR11371. 1 hit.
PfamPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
PIRSFPIRSF000988. DNase_I_euk. 1 hit.
PRINTSPR00130. DNASEI.
SMARTSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMSSF56219. SSF56219. 1 hit.
PROSITEPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283827.
PROP49183.
SOURCESearch...

Entry information

Entry nameDNAS1_MOUSE
AccessionPrimary (citable) accession number: P49183
Secondary accession number(s): O70532
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot