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P49183

- DNAS1_MOUSE

UniProt

P49183 - DNAS1_MOUSE

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Protein

Deoxyribonuclease-1

Gene

Dnase1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Divalent cations. Prefers calcium or magnesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei100 – 1001By similarity
Active sitei156 – 1561By similarity

GO - Molecular functioni

  1. deoxyribonuclease I activity Source: UniProtKB-EC

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Actin-binding, Calcium

Enzyme and pathway databases

BRENDAi3.1.21.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-1 (EC:3.1.21.1)
Alternative name(s):
Deoxyribonuclease I
Short name:
DNase I
Gene namesi
Name:Dnase1
Synonyms:Dnl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:103157. Dnase1.

Subcellular locationi

Secreted By similarity. Nucleus envelope By similarity
Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope.By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 284262Deoxyribonuclease-1PRO_0000007278Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi123 ↔ 126By similarity
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi195 ↔ 231Essential for enzymatic activityBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP49183.
PaxDbiP49183.
PRIDEiP49183.

PTM databases

PhosphoSiteiP49183.

Expressioni

Tissue specificityi

In striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.

Gene expression databases

BgeeiP49183.
CleanExiMM_DNASE1.
ExpressionAtlasiP49183. baseline and differential.
GenevestigatoriP49183.

Interactioni

Protein-protein interaction databases

IntActiP49183. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP49183.
SMRiP49183. Positions 23-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase I family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46375.
HOGENOMiHOG000059570.
HOVERGENiHBG051368.
InParanoidiP49183.
KOiK11994.
OMAiTSTHCAY.
PhylomeDBiP49183.
TreeFamiTF329541.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR11371. PTHR11371. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
PRINTSiPR00130. DNASEI.
SMARTiSM00476. DNaseIc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49183 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRYTGLMGTL LTLVNLLQLA GTLRIAAFNI RTFGETKMSN ATLSVYFVKI
60 70 80 90 100
LSRYDIAVIQ EVRDSHLVAV GKLLDELNRD KPDTYRYVVS EPLGRKSYKE
110 120 130 140 150
QYLFVYRPDQ VSILDSYQYD DGCEPCGNDT FSREPAIVKF FSPYTEVQEF
160 170 180 190 200
AIVPLHAAPT EAVSEIDALY DVYLDVWQKW GLEDIMFMGD FNAGCSYVTS
210 220 230 240 250
SQWSSIRLRT SPIFQWLIPD SADTTVTSTH CAYDRIVVAG ALLQAAVVPN
260 270 280
SAVPFDFQAE YGLSNQLAEA ISDHYPVEVT LRKI
Length:284
Mass (Da):32,027
Last modified:July 15, 1999 - v2
Checksum:i8682E20515EEB510
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti239 – 2402AG → VR in AAA03710. (PubMed:7857306)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00478 mRNA. Translation: AAA03710.1.
D83038 mRNA. Translation: BAA28622.1.
CCDSiCCDS27913.1.
PIRiJC2526.
RefSeqiNP_034191.3. NM_010061.5.
XP_006521837.1. XM_006521774.1.
XP_006521838.1. XM_006521775.1.
XP_006521839.1. XM_006521776.1.
UniGeneiMm.440565.

Genome annotation databases

EnsembliENSMUST00000006136; ENSMUSP00000006136; ENSMUSG00000005980.
ENSMUST00000120009; ENSMUSP00000113119; ENSMUSG00000005980.
GeneIDi13419.
KEGGimmu:13419.
UCSCiuc007xzh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00478 mRNA. Translation: AAA03710.1 .
D83038 mRNA. Translation: BAA28622.1 .
CCDSi CCDS27913.1.
PIRi JC2526.
RefSeqi NP_034191.3. NM_010061.5.
XP_006521837.1. XM_006521774.1.
XP_006521838.1. XM_006521775.1.
XP_006521839.1. XM_006521776.1.
UniGenei Mm.440565.

3D structure databases

ProteinModelPortali P49183.
SMRi P49183. Positions 23-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P49183. 1 interaction.

PTM databases

PhosphoSitei P49183.

Proteomic databases

MaxQBi P49183.
PaxDbi P49183.
PRIDEi P49183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000006136 ; ENSMUSP00000006136 ; ENSMUSG00000005980 .
ENSMUST00000120009 ; ENSMUSP00000113119 ; ENSMUSG00000005980 .
GeneIDi 13419.
KEGGi mmu:13419.
UCSCi uc007xzh.1. mouse.

Organism-specific databases

CTDi 1773.
MGIi MGI:103157. Dnase1.

Phylogenomic databases

eggNOGi NOG46375.
HOGENOMi HOG000059570.
HOVERGENi HBG051368.
InParanoidi P49183.
KOi K11994.
OMAi TSTHCAY.
PhylomeDBi P49183.
TreeFami TF329541.

Enzyme and pathway databases

BRENDAi 3.1.21.1. 3474.

Miscellaneous databases

NextBioi 283827.
PROi P49183.
SOURCEi Search...

Gene expression databases

Bgeei P49183.
CleanExi MM_DNASE1.
ExpressionAtlasi P49183. baseline and differential.
Genevestigatori P49183.

Family and domain databases

Gene3Di 3.60.10.10. 1 hit.
InterProi IPR018057. Deoxyribonuclease-1_AS.
IPR016202. DNase_I.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view ]
PANTHERi PTHR11371. PTHR11371. 1 hit.
Pfami PF03372. Exo_endo_phos. 1 hit.
[Graphical view ]
PIRSFi PIRSF000988. DNase_I_euk. 1 hit.
PRINTSi PR00130. DNASEI.
SMARTi SM00476. DNaseIc. 1 hit.
[Graphical view ]
SUPFAMi SSF56219. SSF56219. 1 hit.
PROSITEi PS00919. DNASE_I_1. 1 hit.
PS00918. DNASE_I_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic organisation and expression of mouse deoxyribonuclease I."
    Peitsch M.C., Irmler M., French L.E., Tschopp J.
    Biochem. Biophys. Res. Commun. 207:62-68(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Heart.
  2. "Mouse deoxyribonuclease I (DNase I): biochemical and immunological characterization, cDNA structure and tissue distribution."
    Takeshita H., Yasuda T., Nakajima T., Hosomi O., Nakashima Y., Kishi K.
    Biochem. Mol. Biol. Int. 42:65-75(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Kidney and Parotid gland.

Entry informationi

Entry nameiDNAS1_MOUSE
AccessioniPrimary (citable) accession number: P49183
Secondary accession number(s): O70532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3