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P49183

- DNAS1_MOUSE

UniProt

P49183 - DNAS1_MOUSE

Protein

Deoxyribonuclease-1

Gene

Dnase1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization By similarity.By similarity

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

    Cofactori

    Divalent cations. Prefers calcium or magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei100 – 1001By similarity
    Active sitei156 – 1561By similarity

    GO - Molecular functioni

    1. deoxyribonuclease I activity Source: UniProtKB-EC

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Apoptosis

    Keywords - Ligandi

    Actin-binding, Calcium

    Enzyme and pathway databases

    BRENDAi3.1.21.1. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyribonuclease-1 (EC:3.1.21.1)
    Alternative name(s):
    Deoxyribonuclease I
    Short name:
    DNase I
    Gene namesi
    Name:Dnase1
    Synonyms:Dnl1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:103157. Dnase1.

    Subcellular locationi

    Secreted By similarity. Nucleus envelope By similarity
    Note: Secretory protein, stored in zymogen granules and found in the nuclear envelope.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. nuclear envelope Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 284262Deoxyribonuclease-1PRO_0000007278Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi123 ↔ 126By similarity
    Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi195 ↔ 231Essential for enzymatic activityBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP49183.
    PaxDbiP49183.
    PRIDEiP49183.

    PTM databases

    PhosphoSiteiP49183.

    Expressioni

    Tissue specificityi

    In striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.

    Gene expression databases

    BgeeiP49183.
    CleanExiMM_DNASE1.
    GenevestigatoriP49183.

    Interactioni

    Protein-protein interaction databases

    IntActiP49183. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP49183.
    SMRiP49183. Positions 23-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNase I family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG46375.
    HOGENOMiHOG000059570.
    HOVERGENiHBG051368.
    InParanoidiP49183.
    KOiK11994.
    OMAiTSTHCAY.
    PhylomeDBiP49183.
    TreeFamiTF329541.

    Family and domain databases

    Gene3Di3.60.10.10. 1 hit.
    InterProiIPR018057. Deoxyribonuclease-1_AS.
    IPR016202. DNase_I.
    IPR005135. Endo/exonuclease/phosphatase.
    [Graphical view]
    PANTHERiPTHR11371. PTHR11371. 1 hit.
    PfamiPF03372. Exo_endo_phos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
    PRINTSiPR00130. DNASEI.
    SMARTiSM00476. DNaseIc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56219. SSF56219. 1 hit.
    PROSITEiPS00919. DNASE_I_1. 1 hit.
    PS00918. DNASE_I_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49183-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRYTGLMGTL LTLVNLLQLA GTLRIAAFNI RTFGETKMSN ATLSVYFVKI    50
    LSRYDIAVIQ EVRDSHLVAV GKLLDELNRD KPDTYRYVVS EPLGRKSYKE 100
    QYLFVYRPDQ VSILDSYQYD DGCEPCGNDT FSREPAIVKF FSPYTEVQEF 150
    AIVPLHAAPT EAVSEIDALY DVYLDVWQKW GLEDIMFMGD FNAGCSYVTS 200
    SQWSSIRLRT SPIFQWLIPD SADTTVTSTH CAYDRIVVAG ALLQAAVVPN 250
    SAVPFDFQAE YGLSNQLAEA ISDHYPVEVT LRKI 284
    Length:284
    Mass (Da):32,027
    Last modified:July 15, 1999 - v2
    Checksum:i8682E20515EEB510
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti239 – 2402AG → VR in AAA03710. (PubMed:7857306)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00478 mRNA. Translation: AAA03710.1.
    D83038 mRNA. Translation: BAA28622.1.
    CCDSiCCDS27913.1.
    PIRiJC2526.
    RefSeqiNP_034191.3. NM_010061.5.
    XP_006521837.1. XM_006521774.1.
    XP_006521838.1. XM_006521775.1.
    XP_006521839.1. XM_006521776.1.
    UniGeneiMm.440565.

    Genome annotation databases

    EnsembliENSMUST00000006136; ENSMUSP00000006136; ENSMUSG00000005980.
    ENSMUST00000120009; ENSMUSP00000113119; ENSMUSG00000005980.
    GeneIDi13419.
    KEGGimmu:13419.
    UCSCiuc007xzh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00478 mRNA. Translation: AAA03710.1 .
    D83038 mRNA. Translation: BAA28622.1 .
    CCDSi CCDS27913.1.
    PIRi JC2526.
    RefSeqi NP_034191.3. NM_010061.5.
    XP_006521837.1. XM_006521774.1.
    XP_006521838.1. XM_006521775.1.
    XP_006521839.1. XM_006521776.1.
    UniGenei Mm.440565.

    3D structure databases

    ProteinModelPortali P49183.
    SMRi P49183. Positions 23-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P49183. 1 interaction.

    PTM databases

    PhosphoSitei P49183.

    Proteomic databases

    MaxQBi P49183.
    PaxDbi P49183.
    PRIDEi P49183.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006136 ; ENSMUSP00000006136 ; ENSMUSG00000005980 .
    ENSMUST00000120009 ; ENSMUSP00000113119 ; ENSMUSG00000005980 .
    GeneIDi 13419.
    KEGGi mmu:13419.
    UCSCi uc007xzh.1. mouse.

    Organism-specific databases

    CTDi 1773.
    MGIi MGI:103157. Dnase1.

    Phylogenomic databases

    eggNOGi NOG46375.
    HOGENOMi HOG000059570.
    HOVERGENi HBG051368.
    InParanoidi P49183.
    KOi K11994.
    OMAi TSTHCAY.
    PhylomeDBi P49183.
    TreeFami TF329541.

    Enzyme and pathway databases

    BRENDAi 3.1.21.1. 3474.

    Miscellaneous databases

    NextBioi 283827.
    PROi P49183.
    SOURCEi Search...

    Gene expression databases

    Bgeei P49183.
    CleanExi MM_DNASE1.
    Genevestigatori P49183.

    Family and domain databases

    Gene3Di 3.60.10.10. 1 hit.
    InterProi IPR018057. Deoxyribonuclease-1_AS.
    IPR016202. DNase_I.
    IPR005135. Endo/exonuclease/phosphatase.
    [Graphical view ]
    PANTHERi PTHR11371. PTHR11371. 1 hit.
    Pfami PF03372. Exo_endo_phos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000988. DNase_I_euk. 1 hit.
    PRINTSi PR00130. DNASEI.
    SMARTi SM00476. DNaseIc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56219. SSF56219. 1 hit.
    PROSITEi PS00919. DNASE_I_1. 1 hit.
    PS00918. DNASE_I_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organisation and expression of mouse deoxyribonuclease I."
      Peitsch M.C., Irmler M., French L.E., Tschopp J.
      Biochem. Biophys. Res. Commun. 207:62-68(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Heart.
    2. "Mouse deoxyribonuclease I (DNase I): biochemical and immunological characterization, cDNA structure and tissue distribution."
      Takeshita H., Yasuda T., Nakajima T., Hosomi O., Nakashima Y., Kishi K.
      Biochem. Mol. Biol. Int. 42:65-75(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Kidney and Parotid gland.

    Entry informationi

    Entry nameiDNAS1_MOUSE
    AccessioniPrimary (citable) accession number: P49183
    Secondary accession number(s): O70532
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3