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P49182 (HEP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heparin cofactor 2
Alternative name(s):
Heparin cofactor II
Short name=HC-II
Protease inhibitor leuserpin-2
Serpin D1
Gene names
Name:Serpind1
Synonyms:Hcf2, Hcii
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Tissue specificity

Expressed predominantly in liver.

Domain

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition By similarity.

Post-translational modification

N-glycosylated; different glycan composition appears to lead to two forms of this protein (68 and 72 kDa).

Sequence similarities

Belongs to the serpin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.1
Chain24 – 478455Heparin cofactor 2
PRO_0000032495

Regions

Repeat54 – 64111
Repeat68 – 78112
Region54 – 78252 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like)
Region171 – 19121Glycosaminoglycan-binding site By similarity

Sites

Site442 – 4432Reactive bond By similarity

Amino acid modifications

Modified residue601Sulfotyrosine By similarity
Modified residue731Sulfotyrosine By similarity
Glycosylation301N-linked (GlcNAc...) Ref.3
Glycosylation1671N-linked (GlcNAc...) Ref.3
Glycosylation3661N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P49182 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 7234711F0083865D

FASTA47854,497
        10         20         30         40         50         60 
MKHPLCTLLS LITFMCIGSK GLAEQLTNEN LTTSFLPANF HKENTVTNDW IPEGEEDEDY 

        70         80         90        100        110        120 
LDLEKLLGED DDYIYIIDAV SPTDSESSAG NILQLFQGKS RIQRLNILNA KFAFNLYRVL 

       130        140        150        160        170        180 
KDQATTSDNL FIAPVGISTA MGMISLGLRG ETHEEVHSVL HFRDFVNASS KYEVTTIHNL 

       190        200        210        220        230        240 
FRKLTHRLFR RNFGYTLRSV NGLYIQKQFP IREDFKAAMR EFYFAEAQEA NFPDPAFISK 

       250        260        270        280        290        300 
ANNHILKLTK GLIKEALENI DPATQMLILN CIYFKGTWVN KFPVEMTHNH NFRLNEREVV 

       310        320        330        340        350        360 
KVSMMQTKGN FLAANDQELD CDILQLEYVG GISMLIVVPR KLSGMKTLEA QLTPQVVERW 

       370        380        390        400        410        420 
QKSMTNRTRE VLLPKFKLEK NYNLVEVLKS MGITKLFNKN GNMSGISDQR IAIDLFKHQS 

       430        440        450        460        470 
TITVNEEGTQ AAAVTTVGFM PLSTQVRFTV DRPFLFLVYE HRTSCLLFMG KVTNPAKS 

« Hide

References

« Hide 'large scale' references
[1]"Murine heparin cofactor II: purification, cDNA sequence, expression, and gene structure."
Zhang G.S., Mehringer J.H., van Deerlin V.M.D., Kozak C.A., Tollefsen D.M.
Biochemistry 33:3632-3642(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-44.
Strain: BALB/c.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[3]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-30 AND ASN-167.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U07425 mRNA. Translation: AAA18452.1.
BC034543 mRNA. Translation: AAH34543.1.
CCDSCCDS28000.1.
PIRA54248.
RefSeqNP_032249.3. NM_008223.3.
XP_006521859.1. XM_006521796.1.
UniGeneMm.6609.

3D structure databases

ProteinModelPortalP49182.
SMRP49182. Positions 54-478.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000023450.

Protein family/group databases

MEROPSI04.019.

PTM databases

PhosphoSiteP49182.

Proteomic databases

MaxQBP49182.
PaxDbP49182.
PRIDEP49182.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023450; ENSMUSP00000023450; ENSMUSG00000022766.
ENSMUST00000161034; ENSMUSP00000125507; ENSMUSG00000022766.
GeneID15160.
KEGGmmu:15160.
UCSCuc007yks.2. mouse.

Organism-specific databases

CTD3053.
MGIMGI:96051. Serpind1.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00750000117478.
HOGENOMHOG000294159.
HOVERGENHBG101242.
InParanoidP49182.
KOK03912.
OMASDQRITI.
OrthoDBEOG7327PB.
PhylomeDBP49182.
TreeFamTF343094.

Gene expression databases

BgeeP49182.
GenevestigatorP49182.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287648.
PROP49182.
SOURCESearch...

Entry information

Entry nameHEP2_MOUSE
AccessionPrimary (citable) accession number: P49182
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot