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P49182

- HEP2_MOUSE

UniProt

P49182 - HEP2_MOUSE

Protein

Heparin cofactor 2

Gene

Serpind1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei442 – 4432Reactive bondBy similarity

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB-KW
    2. serine-type endopeptidase inhibitor activity Source: RefGenome

    GO - Biological processi

    1. blood coagulation Source: UniProtKB-KW
    2. negative regulation of endopeptidase activity Source: RefGenome
    3. regulation of proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Keywords - Biological processi

    Blood coagulation, Hemostasis

    Keywords - Ligandi

    Heparin-binding

    Protein family/group databases

    MEROPSiI04.019.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heparin cofactor 2
    Alternative name(s):
    Heparin cofactor II
    Short name:
    HC-II
    Protease inhibitor leuserpin-2
    Serpin D1
    Gene namesi
    Name:Serpind1
    Synonyms:Hcf2, Hcii
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:96051. Serpind1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 478455Heparin cofactor 2PRO_0000032495Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi30 – 301N-linked (GlcNAc...)1 Publication
    Modified residuei60 – 601SulfotyrosineBy similarity
    Modified residuei73 – 731SulfotyrosineBy similarity
    Glycosylationi167 – 1671N-linked (GlcNAc...)1 Publication
    Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated; different glycan composition appears to lead to two forms of this protein (68 and 72 kDa).1 Publication

    Keywords - PTMi

    Glycoprotein, Sulfation

    Proteomic databases

    MaxQBiP49182.
    PaxDbiP49182.
    PRIDEiP49182.

    PTM databases

    PhosphoSiteiP49182.

    Expressioni

    Tissue specificityi

    Expressed predominantly in liver.

    Gene expression databases

    BgeeiP49182.
    GenevestigatoriP49182.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000023450.

    Structurei

    3D structure databases

    ProteinModelPortaliP49182.
    SMRiP49182. Positions 54-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati54 – 64111Add
    BLAST
    Repeati68 – 78112Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 78252 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like)Add
    BLAST
    Regioni171 – 19121Glycosaminoglycan-binding siteBy similarityAdd
    BLAST

    Domaini

    The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.By similarity

    Sequence similaritiesi

    Belongs to the serpin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG4826.
    GeneTreeiENSGT00750000117478.
    HOGENOMiHOG000294159.
    HOVERGENiHBG101242.
    InParanoidiP49182.
    KOiK03912.
    OMAiSDQRITI.
    OrthoDBiEOG7327PB.
    PhylomeDBiP49182.
    TreeFamiTF343094.

    Family and domain databases

    InterProiIPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 1 hit.
    PROSITEiPS00284. SERPIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49182-1 [UniParc]FASTAAdd to Basket

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    MKHPLCTLLS LITFMCIGSK GLAEQLTNEN LTTSFLPANF HKENTVTNDW    50
    IPEGEEDEDY LDLEKLLGED DDYIYIIDAV SPTDSESSAG NILQLFQGKS 100
    RIQRLNILNA KFAFNLYRVL KDQATTSDNL FIAPVGISTA MGMISLGLRG 150
    ETHEEVHSVL HFRDFVNASS KYEVTTIHNL FRKLTHRLFR RNFGYTLRSV 200
    NGLYIQKQFP IREDFKAAMR EFYFAEAQEA NFPDPAFISK ANNHILKLTK 250
    GLIKEALENI DPATQMLILN CIYFKGTWVN KFPVEMTHNH NFRLNEREVV 300
    KVSMMQTKGN FLAANDQELD CDILQLEYVG GISMLIVVPR KLSGMKTLEA 350
    QLTPQVVERW QKSMTNRTRE VLLPKFKLEK NYNLVEVLKS MGITKLFNKN 400
    GNMSGISDQR IAIDLFKHQS TITVNEEGTQ AAAVTTVGFM PLSTQVRFTV 450
    DRPFLFLVYE HRTSCLLFMG KVTNPAKS 478
    Length:478
    Mass (Da):54,497
    Last modified:February 1, 1996 - v1
    Checksum:i7234711F0083865D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07425 mRNA. Translation: AAA18452.1.
    BC034543 mRNA. Translation: AAH34543.1.
    CCDSiCCDS28000.1.
    PIRiA54248.
    RefSeqiNP_032249.3. NM_008223.3.
    XP_006521859.1. XM_006521796.1.
    UniGeneiMm.6609.

    Genome annotation databases

    EnsembliENSMUST00000023450; ENSMUSP00000023450; ENSMUSG00000022766.
    ENSMUST00000161034; ENSMUSP00000125507; ENSMUSG00000022766.
    GeneIDi15160.
    KEGGimmu:15160.
    UCSCiuc007yks.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07425 mRNA. Translation: AAA18452.1 .
    BC034543 mRNA. Translation: AAH34543.1 .
    CCDSi CCDS28000.1.
    PIRi A54248.
    RefSeqi NP_032249.3. NM_008223.3.
    XP_006521859.1. XM_006521796.1.
    UniGenei Mm.6609.

    3D structure databases

    ProteinModelPortali P49182.
    SMRi P49182. Positions 54-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000023450.

    Protein family/group databases

    MEROPSi I04.019.

    PTM databases

    PhosphoSitei P49182.

    Proteomic databases

    MaxQBi P49182.
    PaxDbi P49182.
    PRIDEi P49182.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023450 ; ENSMUSP00000023450 ; ENSMUSG00000022766 .
    ENSMUST00000161034 ; ENSMUSP00000125507 ; ENSMUSG00000022766 .
    GeneIDi 15160.
    KEGGi mmu:15160.
    UCSCi uc007yks.2. mouse.

    Organism-specific databases

    CTDi 3053.
    MGIi MGI:96051. Serpind1.

    Phylogenomic databases

    eggNOGi COG4826.
    GeneTreei ENSGT00750000117478.
    HOGENOMi HOG000294159.
    HOVERGENi HBG101242.
    InParanoidi P49182.
    KOi K03912.
    OMAi SDQRITI.
    OrthoDBi EOG7327PB.
    PhylomeDBi P49182.
    TreeFami TF343094.

    Miscellaneous databases

    NextBioi 287648.
    PROi P49182.
    SOURCEi Search...

    Gene expression databases

    Bgeei P49182.
    Genevestigatori P49182.

    Family and domain databases

    InterProi IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 1 hit.
    PROSITEi PS00284. SERPIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine heparin cofactor II: purification, cDNA sequence, expression, and gene structure."
      Zhang G.S., Mehringer J.H., van Deerlin V.M.D., Kozak C.A., Tollefsen D.M.
      Biochemistry 33:3632-3642(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-44.
      Strain: BALB/c.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-30 AND ASN-167.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiHEP2_MOUSE
    AccessioniPrimary (citable) accession number: P49182
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3