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Protein

Heparin cofactor 2

Gene

Serpind1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei442 – 4432Reactive bondBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_286713. Common Pathway of Fibrin Clot Formation.
REACT_340184. Intrinsic Pathway of Fibrin Clot Formation.

Protein family/group databases

MEROPSiI04.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparin cofactor 2
Alternative name(s):
Heparin cofactor II
Short name:
HC-II
Protease inhibitor leuserpin-2
Serpin D1
Gene namesi
Name:Serpind1
Synonyms:Hcf2, Hcii
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:96051. Serpind1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 478455Heparin cofactor 2PRO_0000032495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...)1 Publication
Modified residuei60 – 601SulfotyrosineBy similarity
Modified residuei73 – 731SulfotyrosineBy similarity
Glycosylationi167 – 1671N-linked (GlcNAc...)1 Publication
Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated; different glycan composition appears to lead to two forms of this protein (68 and 72 kDa).1 Publication

Keywords - PTMi

Glycoprotein, Sulfation

Proteomic databases

MaxQBiP49182.
PaxDbiP49182.
PRIDEiP49182.

PTM databases

PhosphoSiteiP49182.

Expressioni

Tissue specificityi

Expressed predominantly in liver.

Gene expression databases

BgeeiP49182.
GenevestigatoriP49182.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023450.

Structurei

3D structure databases

ProteinModelPortaliP49182.
SMRiP49182. Positions 54-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati54 – 64111Add
BLAST
Repeati68 – 78112Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 78252 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like)Add
BLAST
Regioni171 – 19121Glycosaminoglycan-binding siteBy similarityAdd
BLAST

Domaini

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000294159.
HOVERGENiHBG101242.
InParanoidiP49182.
KOiK03912.
OMAiSDQRITI.
OrthoDBiEOG7327PB.
PhylomeDBiP49182.
TreeFamiTF343094.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHPLCTLLS LITFMCIGSK GLAEQLTNEN LTTSFLPANF HKENTVTNDW
60 70 80 90 100
IPEGEEDEDY LDLEKLLGED DDYIYIIDAV SPTDSESSAG NILQLFQGKS
110 120 130 140 150
RIQRLNILNA KFAFNLYRVL KDQATTSDNL FIAPVGISTA MGMISLGLRG
160 170 180 190 200
ETHEEVHSVL HFRDFVNASS KYEVTTIHNL FRKLTHRLFR RNFGYTLRSV
210 220 230 240 250
NGLYIQKQFP IREDFKAAMR EFYFAEAQEA NFPDPAFISK ANNHILKLTK
260 270 280 290 300
GLIKEALENI DPATQMLILN CIYFKGTWVN KFPVEMTHNH NFRLNEREVV
310 320 330 340 350
KVSMMQTKGN FLAANDQELD CDILQLEYVG GISMLIVVPR KLSGMKTLEA
360 370 380 390 400
QLTPQVVERW QKSMTNRTRE VLLPKFKLEK NYNLVEVLKS MGITKLFNKN
410 420 430 440 450
GNMSGISDQR IAIDLFKHQS TITVNEEGTQ AAAVTTVGFM PLSTQVRFTV
460 470
DRPFLFLVYE HRTSCLLFMG KVTNPAKS
Length:478
Mass (Da):54,497
Last modified:February 1, 1996 - v1
Checksum:i7234711F0083865D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07425 mRNA. Translation: AAA18452.1.
BC034543 mRNA. Translation: AAH34543.1.
CCDSiCCDS28000.1.
PIRiA54248.
RefSeqiNP_032249.3. NM_008223.3.
XP_006521859.1. XM_006521796.1.
UniGeneiMm.6609.

Genome annotation databases

EnsembliENSMUST00000023450; ENSMUSP00000023450; ENSMUSG00000022766.
ENSMUST00000161034; ENSMUSP00000125507; ENSMUSG00000022766.
GeneIDi15160.
KEGGimmu:15160.
UCSCiuc007yks.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07425 mRNA. Translation: AAA18452.1.
BC034543 mRNA. Translation: AAH34543.1.
CCDSiCCDS28000.1.
PIRiA54248.
RefSeqiNP_032249.3. NM_008223.3.
XP_006521859.1. XM_006521796.1.
UniGeneiMm.6609.

3D structure databases

ProteinModelPortaliP49182.
SMRiP49182. Positions 54-478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023450.

Protein family/group databases

MEROPSiI04.019.

PTM databases

PhosphoSiteiP49182.

Proteomic databases

MaxQBiP49182.
PaxDbiP49182.
PRIDEiP49182.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023450; ENSMUSP00000023450; ENSMUSG00000022766.
ENSMUST00000161034; ENSMUSP00000125507; ENSMUSG00000022766.
GeneIDi15160.
KEGGimmu:15160.
UCSCiuc007yks.2. mouse.

Organism-specific databases

CTDi3053.
MGIiMGI:96051. Serpind1.

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000294159.
HOVERGENiHBG101242.
InParanoidiP49182.
KOiK03912.
OMAiSDQRITI.
OrthoDBiEOG7327PB.
PhylomeDBiP49182.
TreeFamiTF343094.

Enzyme and pathway databases

ReactomeiREACT_286713. Common Pathway of Fibrin Clot Formation.
REACT_340184. Intrinsic Pathway of Fibrin Clot Formation.

Miscellaneous databases

NextBioi287648.
PROiP49182.
SOURCEiSearch...

Gene expression databases

BgeeiP49182.
GenevestigatoriP49182.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine heparin cofactor II: purification, cDNA sequence, expression, and gene structure."
    Zhang G.S., Mehringer J.H., van Deerlin V.M.D., Kozak C.A., Tollefsen D.M.
    Biochemistry 33:3632-3642(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-44.
    Strain: BALB/c.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-30 AND ASN-167.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiHEP2_MOUSE
AccessioniPrimary (citable) accession number: P49182
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 27, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.