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P49182

- HEP2_MOUSE

UniProt

P49182 - HEP2_MOUSE

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Protein
Heparin cofactor 2
Gene
Serpind1, Hcf2, Hcii
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei442 – 4432Reactive bond By similarity

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW
  2. serine-type endopeptidase inhibitor activity Source: RefGenome

GO - Biological processi

  1. blood coagulation Source: UniProtKB-KW
  2. negative regulation of endopeptidase activity Source: RefGenome
  3. regulation of proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Blood coagulation, Hemostasis

Keywords - Ligandi

Heparin-binding

Protein family/group databases

MEROPSiI04.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparin cofactor 2
Alternative name(s):
Heparin cofactor II
Short name:
HC-II
Protease inhibitor leuserpin-2
Serpin D1
Gene namesi
Name:Serpind1
Synonyms:Hcf2, Hcii
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:96051. Serpind1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 Publication
Add
BLAST
Chaini24 – 478455Heparin cofactor 2
PRO_0000032495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...)1 Publication
Modified residuei60 – 601Sulfotyrosine By similarity
Modified residuei73 – 731Sulfotyrosine By similarity
Glycosylationi167 – 1671N-linked (GlcNAc...)1 Publication
Glycosylationi366 – 3661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi402 – 4021N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated; different glycan composition appears to lead to two forms of this protein (68 and 72 kDa).

Keywords - PTMi

Glycoprotein, Sulfation

Proteomic databases

MaxQBiP49182.
PaxDbiP49182.
PRIDEiP49182.

PTM databases

PhosphoSiteiP49182.

Expressioni

Tissue specificityi

Expressed predominantly in liver.

Gene expression databases

BgeeiP49182.
GenevestigatoriP49182.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023450.

Structurei

3D structure databases

ProteinModelPortaliP49182.
SMRiP49182. Positions 54-478.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati54 – 64111
Add
BLAST
Repeati68 – 78112
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 78252 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like)
Add
BLAST
Regioni171 – 19121Glycosaminoglycan-binding site By similarity
Add
BLAST

Domaini

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition By similarity.

Sequence similaritiesi

Belongs to the serpin family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00750000117478.
HOGENOMiHOG000294159.
HOVERGENiHBG101242.
InParanoidiP49182.
KOiK03912.
OMAiSDQRITI.
OrthoDBiEOG7327PB.
PhylomeDBiP49182.
TreeFamiTF343094.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49182-1 [UniParc]FASTAAdd to Basket

« Hide

MKHPLCTLLS LITFMCIGSK GLAEQLTNEN LTTSFLPANF HKENTVTNDW    50
IPEGEEDEDY LDLEKLLGED DDYIYIIDAV SPTDSESSAG NILQLFQGKS 100
RIQRLNILNA KFAFNLYRVL KDQATTSDNL FIAPVGISTA MGMISLGLRG 150
ETHEEVHSVL HFRDFVNASS KYEVTTIHNL FRKLTHRLFR RNFGYTLRSV 200
NGLYIQKQFP IREDFKAAMR EFYFAEAQEA NFPDPAFISK ANNHILKLTK 250
GLIKEALENI DPATQMLILN CIYFKGTWVN KFPVEMTHNH NFRLNEREVV 300
KVSMMQTKGN FLAANDQELD CDILQLEYVG GISMLIVVPR KLSGMKTLEA 350
QLTPQVVERW QKSMTNRTRE VLLPKFKLEK NYNLVEVLKS MGITKLFNKN 400
GNMSGISDQR IAIDLFKHQS TITVNEEGTQ AAAVTTVGFM PLSTQVRFTV 450
DRPFLFLVYE HRTSCLLFMG KVTNPAKS 478
Length:478
Mass (Da):54,497
Last modified:February 1, 1996 - v1
Checksum:i7234711F0083865D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07425 mRNA. Translation: AAA18452.1.
BC034543 mRNA. Translation: AAH34543.1.
CCDSiCCDS28000.1.
PIRiA54248.
RefSeqiNP_032249.3. NM_008223.3.
XP_006521859.1. XM_006521796.1.
UniGeneiMm.6609.

Genome annotation databases

EnsembliENSMUST00000023450; ENSMUSP00000023450; ENSMUSG00000022766.
ENSMUST00000161034; ENSMUSP00000125507; ENSMUSG00000022766.
GeneIDi15160.
KEGGimmu:15160.
UCSCiuc007yks.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07425 mRNA. Translation: AAA18452.1 .
BC034543 mRNA. Translation: AAH34543.1 .
CCDSi CCDS28000.1.
PIRi A54248.
RefSeqi NP_032249.3. NM_008223.3.
XP_006521859.1. XM_006521796.1.
UniGenei Mm.6609.

3D structure databases

ProteinModelPortali P49182.
SMRi P49182. Positions 54-478.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000023450.

Protein family/group databases

MEROPSi I04.019.

PTM databases

PhosphoSitei P49182.

Proteomic databases

MaxQBi P49182.
PaxDbi P49182.
PRIDEi P49182.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023450 ; ENSMUSP00000023450 ; ENSMUSG00000022766 .
ENSMUST00000161034 ; ENSMUSP00000125507 ; ENSMUSG00000022766 .
GeneIDi 15160.
KEGGi mmu:15160.
UCSCi uc007yks.2. mouse.

Organism-specific databases

CTDi 3053.
MGIi MGI:96051. Serpind1.

Phylogenomic databases

eggNOGi COG4826.
GeneTreei ENSGT00750000117478.
HOGENOMi HOG000294159.
HOVERGENi HBG101242.
InParanoidi P49182.
KOi K03912.
OMAi SDQRITI.
OrthoDBi EOG7327PB.
PhylomeDBi P49182.
TreeFami TF343094.

Miscellaneous databases

NextBioi 287648.
PROi P49182.
SOURCEi Search...

Gene expression databases

Bgeei P49182.
Genevestigatori P49182.

Family and domain databases

InterProi IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view ]
PANTHERi PTHR11461. PTHR11461. 1 hit.
Pfami PF00079. Serpin. 1 hit.
[Graphical view ]
SMARTi SM00093. SERPIN. 1 hit.
[Graphical view ]
SUPFAMi SSF56574. SSF56574. 1 hit.
PROSITEi PS00284. SERPIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine heparin cofactor II: purification, cDNA sequence, expression, and gene structure."
    Zhang G.S., Mehringer J.H., van Deerlin V.M.D., Kozak C.A., Tollefsen D.M.
    Biochemistry 33:3632-3642(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-44.
    Strain: BALB/c.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-30 AND ASN-167.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiHEP2_MOUSE
AccessioniPrimary (citable) accession number: P49182
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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