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Protein

Guanine nucleotide-binding protein subunit beta

Gene

GB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Together with GCR1 and GPA1, acts as a negative regulator of ABA during seed germination and early seedling development. The heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi probably by modulating cell wall-related genes expression (e.g. lower xylose content in cell walls); involved in resistance to fungal pathogens such as Alternaria brassicicola and Fusarium oxysporum. Modulates root architecture (e.g. lateral root formation). Acts with XGL3 in the positive regulation of root waving and root skewing.7 Publications

GO - Molecular functioni

  • GTPase activity Source: TAIR
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • defense response to fungus Source: TAIR
  • defense response to fungus, incompatible interaction Source: TAIR
  • endoplasmic reticulum unfolded protein response Source: TAIR
  • fruit development Source: TAIR
  • jasmonic acid mediated signaling pathway Source: TAIR
  • lateral root development Source: TAIR
  • negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • organ morphogenesis Source: TAIR
  • reactive oxygen species metabolic process Source: TAIR
  • regulation of root development Source: UniProtKB
  • response to ethylene Source: TAIR
  • response to extracellular stimulus Source: TAIR
  • root development Source: TAIR
  • seed germination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Abscisic acid signaling pathway, Plant defense

Enzyme and pathway databases

BioCyciARA:AT4G34460-MONOMER.
ReactomeiR-ATH-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta
Alternative name(s):
AGB1
transducin
Gene namesi
Name:GB1
Ordered Locus Names:At4g34460
ORF Names:T4L20.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G34460.

Subcellular locationi

GO - Cellular componenti

  • Cul4-RING E3 ubiquitin ligase complex Source: TAIR
  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum Source: TAIR
  • heterotrimeric G-protein complex Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Hypersensitivity to abscisic acid (ABA) and glucose (Glc) during and after seed germination. Shorter hypocotyls and abnormal roots architecture; more auxin-induced lateral roots. Enhanced susceptibility to necrotrophic and vascular pathogenic fungi, such as Alternaria brassicicola, Plectosphaerella cucumerina and Fusarium oxysporum associated with a disturbed expression of genes involved in cell wall metabolism. Longer and wider primary roots with faster growth. Severely compromised root waving and abnormal root skewing response. Hypersensitivity to ethylene (ACC).7 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Guanine nucleotide-binding protein subunit betaPRO_0000127722Add
BLAST

Proteomic databases

PaxDbiP49177.
PRIDEiP49177.

Expressioni

Tissue specificityi

Expressed in seedlings (especially at the hypocotyl/root junction), roots, leaves (restricted to veins and guard cells), and flowers. Also present in hydathods.2 Publications

Developmental stagei

In flowers, mostly expressed in stigma and pollen, and moderately present in sepals and stamen filaments. In siliques, observed at both ends, gradually disappearing toward the center.1 Publication

Inductioni

Induced upon imbibition. Induced locally by Alternaria brassicicola but systemically by Fusarium oxysporum.2 Publications

Gene expression databases

ExpressionAtlasiP49177. baseline and differential.
GenevisibleiP49177. AT.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with the gamma subunits GG1 and GG2. The dimers GB1-GG1 and GB1-GG2 interact with NDL1, NDL2 and NDL3. Interacts with WNK8. Interacts with XLG2 (PubMed:11121078, PubMed:11513956, PubMed:17158913, PubMed:17468261, PubMed:19825634, PubMed:19948787, PubMed:22940907). Interacts with RACK1A, RACK1B and RACK1C (PubMed:25731164).8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GG1Q9FDX97EBI-1632851,EBI-1750878
GG2Q93V472EBI-1632851,EBI-1751115

Protein-protein interaction databases

BioGridi14879. 95 interactions.
DIPiDIP-40108N.
IntActiP49177. 6 interactions.
STRINGi3702.AT4G34460.1.

Structurei

3D structure databases

ProteinModelPortaliP49177.
SMRiP49177. Positions 22-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati63 – 9331WD 1Add
BLAST
Repeati105 – 13531WD 2Add
BLAST
Repeati154 – 18532WD 3Add
BLAST
Repeati202 – 23332WD 4Add
BLAST
Repeati246 – 27631WD 5Add
BLAST
Repeati292 – 32332WD 6Add
BLAST
Repeati339 – 36931WD 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi220 – 23516DWD box 1Add
BLAST
Motifi263 – 27816DWD box 1Add
BLAST

Domaini

The DWD box is required for interaction with DDB1A.By similarity

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
HOGENOMiHOG000176356.
InParanoidiP49177.
KOiK04536.
OMAiQVYQPHG.
PhylomeDBiP49177.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P49177-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSELKERH AVATETVNNL RDQLRQRRLQ LLDTDVARYS AAQGRTRVSF
60 70 80 90 100
GATDLVCCRT LQGHTGKVYS LDWTPERNRI VSASQDGRLI VWNALTSQKT
110 120 130 140 150
HAIKLPCAWV MTCAFSPNGQ SVACGGLDSV CSIFSLSSTA DKDGTVPVSR
160 170 180 190 200
MLTGHRGYVS CCQYVPNEDA HLITSSGDQT CILWDVTTGL KTSVFGGEFQ
210 220 230 240 250
SGHTADVLSV SISGSNPNWF ISGSCDSTAR LWDTRAASRA VRTFHGHEGD
260 270 280 290 300
VNTVKFFPDG YRFGTGSDDG TCRLYDIRTG HQLQVYQPHG DGENGPVTSI
310 320 330 340 350
AFSVSGRLLF AGYASNNTCY VWDTLLGEVV LDLGLQQDSH RNRISCLGLS
360 370
ADGSALCTGS WDSNLKIWAF GGHRRVI
Length:377
Mass (Da):41,006
Last modified:February 1, 1996 - v1
Checksum:i06673A2F707A2F44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12232 mRNA. Translation: AAA50445.1.
AL023094 Genomic DNA. Translation: CAA18825.1.
AL161585 Genomic DNA. Translation: CAB80163.1.
CP002687 Genomic DNA. Translation: AEE86381.1.
PIRiT05266.
RefSeqiNP_195172.1. NM_119611.4. [P49177-1]
UniGeneiAt.22855.

Genome annotation databases

EnsemblPlantsiAT4G34460.1; AT4G34460.1; AT4G34460. [P49177-1]
GeneIDi829597.
KEGGiath:AT4G34460.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12232 mRNA. Translation: AAA50445.1.
AL023094 Genomic DNA. Translation: CAA18825.1.
AL161585 Genomic DNA. Translation: CAB80163.1.
CP002687 Genomic DNA. Translation: AEE86381.1.
PIRiT05266.
RefSeqiNP_195172.1. NM_119611.4. [P49177-1]
UniGeneiAt.22855.

3D structure databases

ProteinModelPortaliP49177.
SMRiP49177. Positions 22-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14879. 95 interactions.
DIPiDIP-40108N.
IntActiP49177. 6 interactions.
STRINGi3702.AT4G34460.1.

Proteomic databases

PaxDbiP49177.
PRIDEiP49177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G34460.1; AT4G34460.1; AT4G34460. [P49177-1]
GeneIDi829597.
KEGGiath:AT4G34460.

Organism-specific databases

TAIRiAT4G34460.

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
HOGENOMiHOG000176356.
InParanoidiP49177.
KOiK04536.
OMAiQVYQPHG.
PhylomeDBiP49177.

Enzyme and pathway databases

BioCyciARA:AT4G34460-MONOMER.
ReactomeiR-ATH-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

PROiP49177.

Gene expression databases

ExpressionAtlasiP49177. baseline and differential.
GenevisibleiP49177. AT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of cDNAs encoding guanine nucleotide-binding protein beta-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1)."
    Weiss C.A., Garnaat C.W., Mukai K., Hu Y., Ma H.
    Proc. Natl. Acad. Sci. U.S.A. 91:9554-9558(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Completing the heterotrimer: isolation and characterization of an Arabidopsis thaliana G protein gamma-subunit cDNA."
    Mason M.G., Botella J.R.
    Proc. Natl. Acad. Sci. U.S.A. 97:14784-14788(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GG1.
    Strain: cv. Columbia.
  5. "Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with Gbeta."
    Mason M.G., Botella J.R.
    Biochim. Biophys. Acta 1520:147-153(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GG2.
    Strain: cv. Columbia.
  6. "Plant G protein heterotrimers require dual lipidation motifs of Galpha and Ggamma and do not dissociate upon activation."
    Adjobo-Hermans M.J.W., Goedhart J., Gadella T.W.J. Jr.
    J. Cell Sci. 119:5087-5097(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  7. "G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development."
    Pandey S., Chen J.-G., Jones A.M., Assmann S.M.
    Plant Physiol. 141:243-256(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY IMBIBITION.
    Strain: cv. Columbia.
  8. "Heterotrimeric G protein gamma subunits provide functional selectivity in Gbetagamma dimer signaling in Arabidopsis."
    Trusov Y., Rookes J.E., Tilbrook K., Chakravorty D., Mason M.G., Anderson D., Chen J.-G., Jones A.M., Botella J.R.
    Plant Cell 19:1235-1250(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBUNIT, TISSUE SPECIFICITY, INDUCTION BY ALTERNARIA BRASSICICOLA AND FUSARIUM OXYSPORUM.
    Strain: cv. Columbia.
  9. "Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases."
    Lee J.H., Terzaghi W., Gusmaroli G., Charron J.B., Yoon H.J., Chen H., He Y.J., Xiong Y., Deng X.W.
    Plant Cell 20:152-167(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DWD MOTIF.
  10. "Regulation of root-wave response by extra large and conventional G proteins in Arabidopsis thaliana."
    Pandey S., Monshausen G.B., Ding L., Assmann S.M.
    Plant J. 55:311-322(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  11. "Ggamma1 + Ggamma2 not equal to Gbeta: heterotrimeric G protein Ggamma-deficient mutants do not recapitulate all phenotypes of Gbeta-deficient mutants."
    Trusov Y., Zhang W., Assmann S.M., Botella J.R.
    Plant Physiol. 147:636-649(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  12. "Arabidopsis extra large G-protein 2 (XLG2) interacts with the Gbeta subunit of heterotrimeric G protein and functions in disease resistance."
    Zhu H., Li G.J., Ding L., Cui X., Berg H., Assmann S.M., Xia Y.
    Mol. Plant 2:513-525(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH XLG2.
  13. "Arabidopsis N-MYC DOWNREGULATED-LIKE1, a positive regulator of auxin transport in a G protein-mediated pathway."
    Mudgil Y., Uhrig J.F., Zhou J., Temple B., Jiang K., Jones A.M.
    Plant Cell 21:3591-3609(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH NDL1; NDL2 AND NDL3.
    Strain: cv. Columbia.
  14. "Glucose attenuation of auxin-mediated bimodality in lateral root formation is partly coupled by the heterotrimeric G protein complex."
    Booker K.S., Schwarz J., Garrett M.B., Jones A.M.
    PLoS ONE 5:E12833-E12833(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  15. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  16. "Endocytosis of the seven-transmembrane RGS1 protein activates G-protein-coupled signalling in Arabidopsis."
    Urano D., Phan N., Jones J.C., Yang J., Huang J., Grigston J., Taylor J.P., Jones A.M.
    Nat. Cell Biol. 14:1079-1088(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WNK8.
  17. Cited for: INTERACTION WITH RACK1A; RACK1B AND RACK1C.

Entry informationi

Entry nameiGBB_ARATH
AccessioniPrimary (citable) accession number: P49177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.