P49174 (INVA_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-fructofuranosidase, cell wall isozyme EC=3.2.1.26 Alternative name(s): Invertase Sucrose hydrolase
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › cell wall.
Induction	By wounding and bacterial infection.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	nectar secretion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to wounding Inferred from electronic annotation. Source: EnsemblPlants/Gramene starch metabolic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	inulinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene levanase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 590	562	Beta-fructofuranosidase, cell wall isozyme		PRO_0000033376
Sites
	Active site	68	1	By similarity
Amino acid modifications
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	341	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P49174 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 296455E61E99B892	FASTA	590	65,198
	10 20 30 40 50 60 MGTRPRGVVL APWAVVLVLV LALRLAGASH VIHRSLEAEA APSVPASIVS PLLRTGYHFQ 70 80 90 100 110 120 PPMNWINDPN APLYYKGWYH LFYQYNPKGA VWGNIVWAHS VSRDLINWVA LEPAIYPSIP 130 140 150 160 170 180 SDKYGCWSGS ATILEDGTPA ILYTGIDRAD INYQVQVLAL PKDASDPLLR EWEKPEEYNP 190 200 210 220 230 240 VATPAAGGIN ATQFRDPTTA WRHAGHWRML VGSVRGARGM ALVYRSRDFR KWTKAKHPLH 250 260 270 280 290 300 SAALTGMWEC PDFFPVSGPG LQAGLDTSAP GTKYVLKSSL DLTRYDYYTI GSYDGGKDRY 310 320 330 340 350 360 YPDDPAGDYH HRRRYDYGNY YASKTFYDPV ERRRVLLGWA NESDSVPDDK AKGWAGIHAI 370 380 390 400 410 420 PRKIWLDPTG KQLLQWPIHE VEKLRGKAVS VDAKLVKPGD HFEVTGIATY QADVEVSFEL 430 440 450 460 470 480 ELEAGTSLLE KAEAFDPAYD DDAQKLCGVK GADARGGVGP FGLWVLASAD LQERTAVFFR 490 500 510 520 530 540 VFRDGHGKPK VLMCTDPTKS SLSPDLYKPT FAGFVDADIS SGKITLRSLI DRSVVESFGA 550 560 570 580 590 GGKTCILSRV YPSIAVGKDA HLYVFNNGEV DVTVSGLTAW EMKKPLMNGA

References

[1]	Shanker S. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Black Mexican Sweet.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17695 mRNA. Translation: AAA64487.1.
PIR	T02096.
RefSeq	NP_001105369.1. NM_001111899.1.
UniGene	Zm.409.
3D structure databases
ProteinModelPortal	P49174.
SMR	P49174. Positions 53-588.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542314.
KEGG	zma:542314.
Organism-specific databases
Gramene	P49174.
MaizeGDB	113032.
Phylogenomic databases
KO	K01193.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INVA_MAIZE

Accession

Primary (citable) accession number: P49174

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	March 6, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program