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Protein

2-hydroxymuconate tautomerase

Gene

dmpI

Organism
Pseudomonas sp. (strain CF600)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.

Catalytic activityi

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate.

Pathwayi: toluene degradation

This protein is involved in the pathway toluene degradation, which is part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the pathway toluene degradation and in Xenobiotic degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

BRENDAi5.3.2.6. 5085.
UniPathwayiUPA00273.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxymuconate tautomerase (EC:5.3.2.6)
Alternative name(s):
4-oxalocrotonate tautomerase
Short name:
4-OT
Gene namesi
Name:dmpI
Encoded oniPlasmid pVI1500 Publication
OrganismiPseudomonas sp. (strain CF600)
Taxonomic identifieri79676 [NCBI]
Taxonomic lineageiBacteriaProteobacteria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 63622-hydroxymuconate tautomerasePRO_0000209513Add
BLAST

Interactioni

Subunit structurei

Homohexamer.

Structurei

Secondary structure

1
63
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108Combined sources
Helixi14 – 3219Combined sources
Helixi36 – 383Combined sources
Beta strandi40 – 467Combined sources
Helixi48 – 503Combined sources
Beta strandi51 – 533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OTFX-ray1.90A/B/C/D/E/F2-63[»]
ProteinModelPortaliP49172.
SMRiP49172. Positions 2-60.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP49172.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR004370. 4-oxalocrotonate_tautomerase.
IPR014347. Tautomerase/MIF_sf.
IPR018191. Tautomerase_Pseudo-typ.
[Graphical view]
PfamiPF01361. Tautomerase. 1 hit.
[Graphical view]
SUPFAMiSSF55331. SSF55331. 1 hit.
TIGRFAMsiTIGR00013. taut. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIAQLYIIE GRTDEQKETL IRQVSEAMAN SLDAPLERVR VLITEMPKNH
60
FGIGGEPASK VRR
Length:63
Mass (Da):7,105
Last modified:January 23, 2007 - v2
Checksum:iCEBC26092D1E5342
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60835 Genomic DNA. Translation: CAA43229.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60835 Genomic DNA. Translation: CAA43229.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OTFX-ray1.90A/B/C/D/E/F2-63[»]
ProteinModelPortaliP49172.
SMRiP49172. Positions 2-60.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00273.
BRENDAi5.3.2.6. 5085.

Miscellaneous databases

EvolutionaryTraceiP49172.

Family and domain databases

InterProiIPR004370. 4-oxalocrotonate_tautomerase.
IPR014347. Tautomerase/MIF_sf.
IPR018191. Tautomerase_Pseudo-typ.
[Graphical view]
PfamiPF01361. Tautomerase. 1 hit.
[Graphical view]
SUPFAMiSSF55331. SSF55331. 1 hit.
TIGRFAMsiTIGR00013. taut. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600."
    Shingler V., Marklund U., Powlowski J.
    J. Bacteriol. 174:711-724(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases."
    Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J., Wilson K.S., Wigley D.B.
    Biochemistry 35:792-802(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

Entry informationi

Entry namei4OT_PSEUF
AccessioniPrimary (citable) accession number: P49172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.