Reviewed, UniProtKB/Swiss-Prot P49150 (URTG_DESRO)

Names and origin

Protein names	Recommended name:     Salivary plasminogen activator gamma     EC=3.4.21.68 Alternative name(s):     DSPA gamma
Organism	Desmodus rotundus (Vampire bat)
Taxonomic identifier	9430 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Chiroptera › Microchiroptera › Phyllostomidae › Desmodontinae › Desmodus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably essential to support the feeding habits of this exclusively haematophagous animal. Probable potent thrombolytic agent.
Catalytic activity	Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Secreted
Domain	Kringle Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Chain	37 – 394	358	Salivary plasminogen activator gamma		PRO_0000028343
Regions
	Domain	45 – 126	82	Kringle
	Domain	143 – 393	251	Peptidase S1
Sites
	Active site	189	1	Charge relay system By similarity
	Active site	238	1	Charge relay system By similarity
	Active site	345	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Disulfide bond	45 ↔ 126		By similarity
	Disulfide bond	66 ↔ 108		By similarity
	Disulfide bond	97 ↔ 121		By similarity
	Disulfide bond	131 ↔ 262		By similarity
	Disulfide bond	174 ↔ 190		By similarity
	Disulfide bond	182 ↔ 251		By similarity
	Disulfide bond	276 ↔ 351		By similarity
	Disulfide bond	308 ↔ 324		By similarity
	Disulfide bond	341 ↔ 369		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49150-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9CCD6F52F3D81FCD	FASTA	394	44,105
	10 20 30 40 50 60 MVNTMKTKLL CVLLLCGAVF SLPRQETYRQ LARGSRAYGD PHATCYKDQG VTYRGTWSTS 70 80 90 100 110 120 ESGAQCINWN SNLLIRRTYN GRMPEAVKLG LGNHNYCRNP DGASKPWCYV IKARKFTSES 130 140 150 160 170 180 CSVPVCSKAT CGLRKYKEPQ LHSTGGLFTD ITSHPWQAAI FAQNRRSSGE RFLCGGILIS 190 200 210 220 230 240 SCWVLTAAHC FQERYPPQHL RVVLGRTYRV KPGKEEQTFE VEKCIVHEEF DDDTYNNDIA 250 260 270 280 290 300 LLQLKSGSPQ CAQESDSVRA ICLPEANLQL PDWTECELSG YGKHKSSSPF YSEQLKEGHV 310 320 330 340 350 360 RLYPSSRCTS KFLFNKTVTN NMLCAGDTRS GEIYPNVHDA CQGDSGGPLV CMNDNHMTLL 370 380 390 GIISWGVGCG EKDIPGVYTK VTNYLGWIRD NMRP

References

[1]	"The plasminogen activator family from the salivary gland of the vampire bat Desmodus rotundus: cloning and expression." Kraetzschmar J., Haendler B., Langer G., Boidol W., Bringmann P., Alagon A., Donner P., Schleuning W.-D. Gene 105:229-237(1991) [PubMed: 1937019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Salivary gland.
[2]	"Plasminogen activators from the saliva of Desmodus rotundus (common vampire bat): unique fibrin specificity." Schleuning W.-D., Alagon A., Boidol W., Bringmann P., Petri T., Kraetzschmar J., Haendler B., Langer G., Baldus B., Witt W., Donner P. Ann. N. Y. Acad. Sci. 667:395-403(1992) [PubMed: 1309059] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63990 mRNA. Translation: AAA31595.1.
PIR	JS0600.
3D structure databases
HSSP	HSSP built from PDB template 1A5I based on UniProtKB P98119.
SMR	P49150. Positions 130-392.
ModBase	Search...
Protein family/group databases
MEROPS	S01.239.
Phylogenomic databases
HOVERGEN	P49150.
Enzyme and pathway databases
BRENDA	3.4.21.68. 291428.
Family and domain databases
InterPro	IPR000001. Kringle. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
ProDom	PD000395. Kringle. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URTG_DESRO

Accession

Primary (citable) accession number: P49150

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)