Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic phospholipase A2

Gene

PLA2G4A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1By similarity
Metal bindingi40 – 401Calcium 2By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi43 – 431Calcium 2By similarity
Metal bindingi65 – 651Calcium 1By similarity
Metal bindingi93 – 931Calcium 2By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Calcium 2By similarity
Active sitei228 – 2281NucleophileBy similarity
Active sitei549 – 5491Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_274343. Acyl chain remodelling of PG.
REACT_284564. Acyl chain remodelling of PE.
REACT_292300. Acyl chain remodeling of CL.
REACT_308119. Acyl chain remodelling of PC.
REACT_317392. Acyl chain remodelling of PI.
REACT_329171. Platelet sensitization by LDL.
REACT_336298. Arachidonic acid metabolism.
REACT_337442. Hydrolysis of LPC.
REACT_339620. ADP signalling through P2Y purinoceptor 1.
REACT_341056. phospho-PLA2 pathway.
REACT_343057. Synthesis of PA.
REACT_349427. Acyl chain remodelling of PS.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Chromosome 8

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 748748Cytosolic phospholipase A2PRO_0000187266Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei505 – 5051Phosphoserine; by MAPKBy similarity

Post-translational modificationi

Activated by phosphorylation on a serine residue.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49147.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038517.

Structurei

3D structure databases

ProteinModelPortaliP49147.
SMRiP49147. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2PROSITE-ProRule annotationAdd
BLAST
Domaini138 – 740603PLA2cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257248.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP49147.
KOiK16342.
OMAiETLIHNR.
OrthoDBiEOG73V6JM.
PhylomeDBiP49147.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIDPYQHI VVEHQYSHVF TVTVRKATNV TKGAIGDMLD TPDPYVELFI
60 70 80 90 100
PSAPDCRKRT KHFNNDVNPV WNETFEFILD PNQDNVLEVT LMDANYVMDE
110 120 130 140 150
TLGMATFPIS SLKLGEKKEV QLTFNNVTEM TLELSLEVCS STDLRFSMAL
160 170 180 190 200
CDEEKKFRQQ RKDNIMQSMK SFLGEENSKN LTTSRDVPVI AVLGSGGGFR
210 220 230 240 250
AMVGFAGVMK ALYESGVLDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPKE
260 270 280 290 300
INQELMNSVS HNPLLLLTPQ KVKRYIEALW NKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIHNRMDT TLSDMKEKVS EAQCALPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMSPDLFGS KFFMGTVVKK YSENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSNS QNKGPTMEEE LENIRLKHLV SNDSSDSEDE SQHPKGTENS
460 470 480 490 500
EANEEYQNSS QESWVQRMLM ALVGDSALFN TREGRAGKVH NFMLGLNLNS
510 520 530 540 550
CYPLSPLADL LTQESVEEDE LDAAVADPDE FERIYEPLDV KSKKIHIVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEI LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPN VFDREGLKEC YVFKPKDTSS EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGLPRESK EEKDFADFDI FDDPNTPFST FNFQYPNEAF KRLHDLMEFN
710 720 730 740
TLNNLDVIKQ AMMESIEYRK ENPSRCSVSL SSVEARRFFN KNNLNNHT
Length:748
Mass (Da):84,979
Last modified:February 1, 1996 - v1
Checksum:i996A5256CA032F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10329 mRNA. Translation: AAA53228.1.
PIRiI50699.
RefSeqiNP_990754.1. NM_205423.1.
UniGeneiGga.3723.

Genome annotation databases

EnsembliENSGALT00000008121; ENSGALP00000008107; ENSGALG00000005065.
GeneIDi396394.
KEGGigga:396394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10329 mRNA. Translation: AAA53228.1.
PIRiI50699.
RefSeqiNP_990754.1. NM_205423.1.
UniGeneiGga.3723.

3D structure databases

ProteinModelPortaliP49147.
SMRiP49147. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038517.

Proteomic databases

PaxDbiP49147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000008121; ENSGALP00000008107; ENSGALG00000005065.
GeneIDi396394.
KEGGigga:396394.

Organism-specific databases

CTDi5321.

Phylogenomic databases

eggNOGiNOG257248.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP49147.
KOiK16342.
OMAiETLIHNR.
OrthoDBiEOG73V6JM.
PhylomeDBiP49147.

Enzyme and pathway databases

ReactomeiREACT_274343. Acyl chain remodelling of PG.
REACT_284564. Acyl chain remodelling of PE.
REACT_292300. Acyl chain remodeling of CL.
REACT_308119. Acyl chain remodelling of PC.
REACT_317392. Acyl chain remodelling of PI.
REACT_329171. Platelet sensitization by LDL.
REACT_336298. Arachidonic acid metabolism.
REACT_337442. Hydrolysis of LPC.
REACT_339620. ADP signalling through P2Y purinoceptor 1.
REACT_341056. phospho-PLA2 pathway.
REACT_343057. Synthesis of PA.
REACT_349427. Acyl chain remodelling of PS.

Miscellaneous databases

NextBioi20816436.
PROiP49147.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Delineation of two functionally distinct domains of cytosolic phospholipase A2, a regulatory Ca(2+)-dependent lipid-binding domain and a Ca(2+)-independent catalytic domain."
    Nalefski E.A., Sultzman L.A., Martin D.M., Kriz R.W., Towler P.S., Knopf J.L., Clark J.D.
    J. Biol. Chem. 269:18239-18249(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Entry informationi

Entry nameiPA24A_CHICK
AccessioniPrimary (citable) accession number: P49147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 1, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.