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P49147

- PA24A_CHICK

UniProt

P49147 - PA24A_CHICK

Protein

Cytosolic phospholipase A2

Gene

PLA2G4A

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

    Catalytic activityi

    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi40 – 401Calcium 1By similarity
    Metal bindingi40 – 401Calcium 2By similarity
    Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi43 – 431Calcium 1By similarity
    Metal bindingi43 – 431Calcium 2By similarity
    Metal bindingi65 – 651Calcium 1By similarity
    Metal bindingi93 – 931Calcium 2By similarity
    Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi95 – 951Calcium 2By similarity
    Active sitei228 – 2281NucleophileBy similarity
    Active sitei549 – 5491Proton acceptorBy similarity

    GO - Molecular functioni

    1. calcium-dependent phospholipase A2 activity Source: Ensembl
    2. calcium-dependent phospholipid binding Source: Ensembl
    3. calcium ion binding Source: Ensembl
    4. lysophospholipase activity Source: UniProtKB-EC

    GO - Biological processi

    1. arachidonic acid secretion Source: Ensembl
    2. cellular response to antibiotic Source: Ensembl
    3. icosanoid biosynthetic process Source: Ensembl
    4. phospholipid catabolic process Source: InterPro
    5. regulation of cell proliferation Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_194344. Synthesis of PA.
    REACT_194362. Arachidonic acid metabolism.
    REACT_197168. Acyl chain remodeling of CL.
    REACT_197169. Acyl chain remodelling of PE.
    REACT_197170. Acyl chain remodelling of PS.
    REACT_197172. Hydrolysis of LPC.
    REACT_197175. Acyl chain remodelling of PG.
    REACT_197176. Acyl chain remodelling of PC.
    REACT_197223. Acyl chain remodelling of PI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic phospholipase A2
    Short name:
    cPLA2
    Alternative name(s):
    Phospholipase A2 group IVA
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:PLA2G4A
    Synonyms:CPLA2, PLA2G4
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome 8

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmic vesicle By similarity
    Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    2. cytosol Source: Ensembl
    3. endoplasmic reticulum Source: Ensembl
    4. Golgi apparatus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 748748Cytosolic phospholipase A2PRO_0000187266Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei505 – 5051Phosphoserine; by MAPKBy similarity

    Post-translational modificationi

    Activated by phosphorylation on a serine residue.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP49147.

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000038517.

    Structurei

    3D structure databases

    ProteinModelPortaliP49147.
    SMRiP49147. Positions 13-721.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 106102C2PROSITE-ProRule annotationAdd
    BLAST
    Domaini138 – 740603PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 178178Phospholipid bindingAdd
    BLAST

    Domaini

    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG257248.
    GeneTreeiENSGT00550000074489.
    HOGENOMiHOG000115420.
    HOVERGENiHBG053479.
    KOiK16342.
    OrthoDBiEOG73V6JM.
    PhylomeDBiP49147.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49147-1 [UniParc]FASTAAdd to Basket

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    MSFIDPYQHI VVEHQYSHVF TVTVRKATNV TKGAIGDMLD TPDPYVELFI    50
    PSAPDCRKRT KHFNNDVNPV WNETFEFILD PNQDNVLEVT LMDANYVMDE 100
    TLGMATFPIS SLKLGEKKEV QLTFNNVTEM TLELSLEVCS STDLRFSMAL 150
    CDEEKKFRQQ RKDNIMQSMK SFLGEENSKN LTTSRDVPVI AVLGSGGGFR 200
    AMVGFAGVMK ALYESGVLDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPKE 250
    INQELMNSVS HNPLLLLTPQ KVKRYIEALW NKKSSGQPVT FTDIFGMLIG 300
    ETLIHNRMDT TLSDMKEKVS EAQCALPLFT CLHVKPDVSE LMFADWVEFS 350
    PYEIGMAKYG TFMSPDLFGS KFFMGTVVKK YSENPLHFLM GVWGSAFSIL 400
    FNRVLGVSNS QNKGPTMEEE LENIRLKHLV SNDSSDSEDE SQHPKGTENS 450
    EANEEYQNSS QESWVQRMLM ALVGDSALFN TREGRAGKVH NFMLGLNLNS 500
    CYPLSPLADL LTQESVEEDE LDAAVADPDE FERIYEPLDV KSKKIHIVDS 550
    GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEI LLAEKWAKMN 600
    KLPFPKIDPN VFDREGLKEC YVFKPKDTSS EKDCPTIIHF VLANINFRKY 650
    KAPGLPRESK EEKDFADFDI FDDPNTPFST FNFQYPNEAF KRLHDLMEFN 700
    TLNNLDVIKQ AMMESIEYRK ENPSRCSVSL SSVEARRFFN KNNLNNHT 748
    Length:748
    Mass (Da):84,979
    Last modified:February 1, 1996 - v1
    Checksum:i996A5256CA032F75
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10329 mRNA. Translation: AAA53228.1.
    PIRiI50699.
    RefSeqiNP_990754.1. NM_205423.1.
    UniGeneiGga.3723.

    Genome annotation databases

    EnsembliENSGALT00000008121; ENSGALP00000008107; ENSGALG00000005065.
    GeneIDi396394.
    KEGGigga:396394.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10329 mRNA. Translation: AAA53228.1 .
    PIRi I50699.
    RefSeqi NP_990754.1. NM_205423.1.
    UniGenei Gga.3723.

    3D structure databases

    ProteinModelPortali P49147.
    SMRi P49147. Positions 13-721.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000038517.

    Proteomic databases

    PaxDbi P49147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000008121 ; ENSGALP00000008107 ; ENSGALG00000005065 .
    GeneIDi 396394.
    KEGGi gga:396394.

    Organism-specific databases

    CTDi 5321.

    Phylogenomic databases

    eggNOGi NOG257248.
    GeneTreei ENSGT00550000074489.
    HOGENOMi HOG000115420.
    HOVERGENi HBG053479.
    KOi K16342.
    OrthoDBi EOG73V6JM.
    PhylomeDBi P49147.

    Enzyme and pathway databases

    Reactomei REACT_194344. Synthesis of PA.
    REACT_194362. Arachidonic acid metabolism.
    REACT_197168. Acyl chain remodeling of CL.
    REACT_197169. Acyl chain remodelling of PE.
    REACT_197170. Acyl chain remodelling of PS.
    REACT_197172. Hydrolysis of LPC.
    REACT_197175. Acyl chain remodelling of PG.
    REACT_197176. Acyl chain remodelling of PC.
    REACT_197223. Acyl chain remodelling of PI.

    Miscellaneous databases

    NextBioi 20816436.
    PROi P49147.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Delineation of two functionally distinct domains of cytosolic phospholipase A2, a regulatory Ca(2+)-dependent lipid-binding domain and a Ca(2+)-independent catalytic domain."
      Nalefski E.A., Sultzman L.A., Martin D.M., Kriz R.W., Towler P.S., Knopf J.L., Clark J.D.
      J. Biol. Chem. 269:18239-18249(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

    Entry informationi

    Entry nameiPA24A_CHICK
    AccessioniPrimary (citable) accession number: P49147
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3