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Protein

Cytosolic phospholipase A2

Gene

PLA2G4A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Calcium 1By similarity1
Metal bindingi40Calcium 2By similarity1
Metal bindingi41Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi43Calcium 1By similarity1
Metal bindingi43Calcium 2By similarity1
Metal bindingi65Calcium 1By similarity1
Metal bindingi93Calcium 2By similarity1
Metal bindingi94Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi95Calcium 2By similarity1
Active sitei228NucleophileBy similarity1
Active sitei549Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-GGA-111995. phospho-PLA2 pathway.
R-GGA-1482788. Acyl chain remodelling of PC.
R-GGA-1482798. Acyl chain remodeling of CL.
R-GGA-1482801. Acyl chain remodelling of PS.
R-GGA-1482839. Acyl chain remodelling of PE.
R-GGA-1482922. Acyl chain remodelling of PI.
R-GGA-1482925. Acyl chain remodelling of PG.
R-GGA-1483115. Hydrolysis of LPC.
R-GGA-1483166. Synthesis of PA.
R-GGA-2142753. Arachidonic acid metabolism.
R-GGA-418592. ADP signalling through P2Y purinoceptor 1.
R-GGA-432142. Platelet sensitization by LDL.
R-GGA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 8

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872661 – 748Cytosolic phospholipase A2Add BLAST748

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei505Phosphoserine; by MAPKBy similarity1

Post-translational modificationi

Activated by phosphorylation on a serine residue.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49147.

Expressioni

Gene expression databases

BgeeiENSGALG00000005065.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038517.

Structurei

3D structure databases

ProteinModelPortaliP49147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 106C2PROSITE-ProRule annotationAdd BLAST102
Domaini138 – 740PLA2cPROSITE-ProRule annotationAdd BLAST603

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 178Phospholipid bindingAdd BLAST178

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP49147.
KOiK16342.
OMAiRFSMALC.
OrthoDBiEOG091G0276.
PhylomeDBiP49147.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIDPYQHI VVEHQYSHVF TVTVRKATNV TKGAIGDMLD TPDPYVELFI
60 70 80 90 100
PSAPDCRKRT KHFNNDVNPV WNETFEFILD PNQDNVLEVT LMDANYVMDE
110 120 130 140 150
TLGMATFPIS SLKLGEKKEV QLTFNNVTEM TLELSLEVCS STDLRFSMAL
160 170 180 190 200
CDEEKKFRQQ RKDNIMQSMK SFLGEENSKN LTTSRDVPVI AVLGSGGGFR
210 220 230 240 250
AMVGFAGVMK ALYESGVLDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPKE
260 270 280 290 300
INQELMNSVS HNPLLLLTPQ KVKRYIEALW NKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIHNRMDT TLSDMKEKVS EAQCALPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMSPDLFGS KFFMGTVVKK YSENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSNS QNKGPTMEEE LENIRLKHLV SNDSSDSEDE SQHPKGTENS
460 470 480 490 500
EANEEYQNSS QESWVQRMLM ALVGDSALFN TREGRAGKVH NFMLGLNLNS
510 520 530 540 550
CYPLSPLADL LTQESVEEDE LDAAVADPDE FERIYEPLDV KSKKIHIVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEI LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPN VFDREGLKEC YVFKPKDTSS EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGLPRESK EEKDFADFDI FDDPNTPFST FNFQYPNEAF KRLHDLMEFN
710 720 730 740
TLNNLDVIKQ AMMESIEYRK ENPSRCSVSL SSVEARRFFN KNNLNNHT
Length:748
Mass (Da):84,979
Last modified:February 1, 1996 - v1
Checksum:i996A5256CA032F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10329 mRNA. Translation: AAA53228.1.
PIRiI50699.
RefSeqiNP_990754.1. NM_205423.1.
XP_015146058.1. XM_015290572.1.
UniGeneiGga.36724.
Gga.3723.

Genome annotation databases

EnsembliENSGALT00000079041; ENSGALP00000048216; ENSGALG00000005065.
GeneIDi396394.
KEGGigga:396394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10329 mRNA. Translation: AAA53228.1.
PIRiI50699.
RefSeqiNP_990754.1. NM_205423.1.
XP_015146058.1. XM_015290572.1.
UniGeneiGga.36724.
Gga.3723.

3D structure databases

ProteinModelPortaliP49147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038517.

Proteomic databases

PaxDbiP49147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000079041; ENSGALP00000048216; ENSGALG00000005065.
GeneIDi396394.
KEGGigga:396394.

Organism-specific databases

CTDi5321.

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP49147.
KOiK16342.
OMAiRFSMALC.
OrthoDBiEOG091G0276.
PhylomeDBiP49147.

Enzyme and pathway databases

ReactomeiR-GGA-111995. phospho-PLA2 pathway.
R-GGA-1482788. Acyl chain remodelling of PC.
R-GGA-1482798. Acyl chain remodeling of CL.
R-GGA-1482801. Acyl chain remodelling of PS.
R-GGA-1482839. Acyl chain remodelling of PE.
R-GGA-1482922. Acyl chain remodelling of PI.
R-GGA-1482925. Acyl chain remodelling of PG.
R-GGA-1483115. Hydrolysis of LPC.
R-GGA-1483166. Synthesis of PA.
R-GGA-2142753. Arachidonic acid metabolism.
R-GGA-418592. ADP signalling through P2Y purinoceptor 1.
R-GGA-432142. Platelet sensitization by LDL.
R-GGA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiP49147.

Gene expression databases

BgeeiENSGALG00000005065.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA24A_CHICK
AccessioniPrimary (citable) accession number: P49147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.