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P49147

- PA24A_CHICK

UniProt

P49147 - PA24A_CHICK

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Protein

Cytosolic phospholipase A2

Gene
PLA2G4A, CPLA2, PLA2G4
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1 By similarity
Metal bindingi40 – 401Calcium 2 By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygen By similarity
Metal bindingi43 – 431Calcium 1 By similarity
Metal bindingi43 – 431Calcium 2 By similarity
Metal bindingi65 – 651Calcium 1 By similarity
Metal bindingi93 – 931Calcium 2 By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygen By similarity
Metal bindingi95 – 951Calcium 2 By similarity
Active sitei228 – 2281Nucleophile By similarity
Active sitei549 – 5491Proton acceptor By similarity

GO - Molecular functioni

  1. calcium-dependent phospholipase A2 activity Source: Ensembl
  2. calcium-dependent phospholipid binding Source: Ensembl
  3. calcium ion binding Source: Ensembl
  4. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. arachidonic acid secretion Source: Ensembl
  2. cellular response to antibiotic Source: Ensembl
  3. icosanoid biosynthetic process Source: Ensembl
  4. phospholipid catabolic process Source: InterPro
  5. regulation of cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_194344. Synthesis of PA.
REACT_194362. Arachidonic acid metabolism.
REACT_197168. Acyl chain remodeling of CL.
REACT_197169. Acyl chain remodelling of PE.
REACT_197170. Acyl chain remodelling of PS.
REACT_197172. Hydrolysis of LPC.
REACT_197175. Acyl chain remodelling of PG.
REACT_197176. Acyl chain remodelling of PC.
REACT_197223. Acyl chain remodelling of PI.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 8

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesicle By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion By similarity.

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  2. cytosol Source: Ensembl
  3. endoplasmic reticulum Source: Ensembl
  4. Golgi apparatus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 748748Cytosolic phospholipase A2PRO_0000187266Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei505 – 5051Phosphoserine; by MAPK By similarity

Post-translational modificationi

Activated by phosphorylation on a serine residue By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49147.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038517.

Structurei

3D structure databases

ProteinModelPortaliP49147.
SMRiP49147. Positions 13-721.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2Add
BLAST
Domaini138 – 740603PLA2cAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 PLA2c domain.

Phylogenomic databases

eggNOGiNOG257248.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
KOiK16342.
OrthoDBiEOG73V6JM.
PhylomeDBiP49147.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49147-1 [UniParc]FASTAAdd to Basket

« Hide

MSFIDPYQHI VVEHQYSHVF TVTVRKATNV TKGAIGDMLD TPDPYVELFI    50
PSAPDCRKRT KHFNNDVNPV WNETFEFILD PNQDNVLEVT LMDANYVMDE 100
TLGMATFPIS SLKLGEKKEV QLTFNNVTEM TLELSLEVCS STDLRFSMAL 150
CDEEKKFRQQ RKDNIMQSMK SFLGEENSKN LTTSRDVPVI AVLGSGGGFR 200
AMVGFAGVMK ALYESGVLDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPKE 250
INQELMNSVS HNPLLLLTPQ KVKRYIEALW NKKSSGQPVT FTDIFGMLIG 300
ETLIHNRMDT TLSDMKEKVS EAQCALPLFT CLHVKPDVSE LMFADWVEFS 350
PYEIGMAKYG TFMSPDLFGS KFFMGTVVKK YSENPLHFLM GVWGSAFSIL 400
FNRVLGVSNS QNKGPTMEEE LENIRLKHLV SNDSSDSEDE SQHPKGTENS 450
EANEEYQNSS QESWVQRMLM ALVGDSALFN TREGRAGKVH NFMLGLNLNS 500
CYPLSPLADL LTQESVEEDE LDAAVADPDE FERIYEPLDV KSKKIHIVDS 550
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEI LLAEKWAKMN 600
KLPFPKIDPN VFDREGLKEC YVFKPKDTSS EKDCPTIIHF VLANINFRKY 650
KAPGLPRESK EEKDFADFDI FDDPNTPFST FNFQYPNEAF KRLHDLMEFN 700
TLNNLDVIKQ AMMESIEYRK ENPSRCSVSL SSVEARRFFN KNNLNNHT 748
Length:748
Mass (Da):84,979
Last modified:February 1, 1996 - v1
Checksum:i996A5256CA032F75
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10329 mRNA. Translation: AAA53228.1.
PIRiI50699.
RefSeqiNP_990754.1. NM_205423.1.
UniGeneiGga.3723.

Genome annotation databases

EnsembliENSGALT00000008121; ENSGALP00000008107; ENSGALG00000005065.
GeneIDi396394.
KEGGigga:396394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10329 mRNA. Translation: AAA53228.1 .
PIRi I50699.
RefSeqi NP_990754.1. NM_205423.1.
UniGenei Gga.3723.

3D structure databases

ProteinModelPortali P49147.
SMRi P49147. Positions 13-721.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000038517.

Proteomic databases

PaxDbi P49147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000008121 ; ENSGALP00000008107 ; ENSGALG00000005065 .
GeneIDi 396394.
KEGGi gga:396394.

Organism-specific databases

CTDi 5321.

Phylogenomic databases

eggNOGi NOG257248.
GeneTreei ENSGT00550000074489.
HOGENOMi HOG000115420.
HOVERGENi HBG053479.
KOi K16342.
OrthoDBi EOG73V6JM.
PhylomeDBi P49147.

Enzyme and pathway databases

Reactomei REACT_194344. Synthesis of PA.
REACT_194362. Arachidonic acid metabolism.
REACT_197168. Acyl chain remodeling of CL.
REACT_197169. Acyl chain remodelling of PE.
REACT_197170. Acyl chain remodelling of PS.
REACT_197172. Hydrolysis of LPC.
REACT_197175. Acyl chain remodelling of PG.
REACT_197176. Acyl chain remodelling of PC.
REACT_197223. Acyl chain remodelling of PI.

Miscellaneous databases

NextBioi 20816436.
PROi P49147.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Delineation of two functionally distinct domains of cytosolic phospholipase A2, a regulatory Ca(2+)-dependent lipid-binding domain and a Ca(2+)-independent catalytic domain."
    Nalefski E.A., Sultzman L.A., Martin D.M., Kriz R.W., Towler P.S., Knopf J.L., Clark J.D.
    J. Biol. Chem. 269:18239-18249(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Entry informationi

Entry nameiPA24A_CHICK
AccessioniPrimary (citable) accession number: P49147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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