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P49147

- PA24A_CHICK

UniProt

P49147 - PA24A_CHICK

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Protein

Cytosolic phospholipase A2

Gene

PLA2G4A

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1By similarity
Metal bindingi40 – 401Calcium 2By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi43 – 431Calcium 1By similarity
Metal bindingi43 – 431Calcium 2By similarity
Metal bindingi65 – 651Calcium 1By similarity
Metal bindingi93 – 931Calcium 2By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi95 – 951Calcium 2By similarity
Active sitei228 – 2281NucleophileBy similarity
Active sitei549 – 5491Proton acceptorBy similarity

GO - Molecular functioni

  1. calcium-dependent phospholipase A2 activity Source: Ensembl
  2. calcium-dependent phospholipid binding Source: Ensembl
  3. calcium ion binding Source: Ensembl
  4. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. arachidonic acid secretion Source: Ensembl
  2. cellular response to antibiotic Source: Ensembl
  3. icosanoid biosynthetic process Source: Ensembl
  4. phospholipid catabolic process Source: InterPro
  5. regulation of cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_194344. Synthesis of PA.
REACT_194362. Arachidonic acid metabolism.
REACT_197168. Acyl chain remodeling of CL.
REACT_197169. Acyl chain remodelling of PE.
REACT_197170. Acyl chain remodelling of PS.
REACT_197172. Hydrolysis of LPC.
REACT_197175. Acyl chain remodelling of PG.
REACT_197176. Acyl chain remodelling of PC.
REACT_197223. Acyl chain remodelling of PI.
REACT_236020. Platelet sensitization by LDL.
REACT_241087. ADP signalling through P2Y purinoceptor 1.
REACT_253568. phospho-PLA2 pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 8

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesicle By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. cytosol Source: Ensembl
  3. endoplasmic reticulum Source: Ensembl
  4. Golgi apparatus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 748748Cytosolic phospholipase A2PRO_0000187266Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei505 – 5051Phosphoserine; by MAPKBy similarity

Post-translational modificationi

Activated by phosphorylation on a serine residue.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP49147.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038517.

Structurei

3D structure databases

ProteinModelPortaliP49147.
SMRiP49147. Positions 13-721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2PROSITE-ProRule annotationAdd
BLAST
Domaini138 – 740603PLA2cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid bindingAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257248.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiP49147.
KOiK16342.
OrthoDBiEOG73V6JM.
PhylomeDBiP49147.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49147-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFIDPYQHI VVEHQYSHVF TVTVRKATNV TKGAIGDMLD TPDPYVELFI
60 70 80 90 100
PSAPDCRKRT KHFNNDVNPV WNETFEFILD PNQDNVLEVT LMDANYVMDE
110 120 130 140 150
TLGMATFPIS SLKLGEKKEV QLTFNNVTEM TLELSLEVCS STDLRFSMAL
160 170 180 190 200
CDEEKKFRQQ RKDNIMQSMK SFLGEENSKN LTTSRDVPVI AVLGSGGGFR
210 220 230 240 250
AMVGFAGVMK ALYESGVLDC ATYIAGLSGS TWYMSTLYSH PDFPEKGPKE
260 270 280 290 300
INQELMNSVS HNPLLLLTPQ KVKRYIEALW NKKSSGQPVT FTDIFGMLIG
310 320 330 340 350
ETLIHNRMDT TLSDMKEKVS EAQCALPLFT CLHVKPDVSE LMFADWVEFS
360 370 380 390 400
PYEIGMAKYG TFMSPDLFGS KFFMGTVVKK YSENPLHFLM GVWGSAFSIL
410 420 430 440 450
FNRVLGVSNS QNKGPTMEEE LENIRLKHLV SNDSSDSEDE SQHPKGTENS
460 470 480 490 500
EANEEYQNSS QESWVQRMLM ALVGDSALFN TREGRAGKVH NFMLGLNLNS
510 520 530 540 550
CYPLSPLADL LTQESVEEDE LDAAVADPDE FERIYEPLDV KSKKIHIVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEI LLAEKWAKMN
610 620 630 640 650
KLPFPKIDPN VFDREGLKEC YVFKPKDTSS EKDCPTIIHF VLANINFRKY
660 670 680 690 700
KAPGLPRESK EEKDFADFDI FDDPNTPFST FNFQYPNEAF KRLHDLMEFN
710 720 730 740
TLNNLDVIKQ AMMESIEYRK ENPSRCSVSL SSVEARRFFN KNNLNNHT
Length:748
Mass (Da):84,979
Last modified:February 1, 1996 - v1
Checksum:i996A5256CA032F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10329 mRNA. Translation: AAA53228.1.
PIRiI50699.
RefSeqiNP_990754.1. NM_205423.1.
UniGeneiGga.3723.

Genome annotation databases

EnsembliENSGALT00000008121; ENSGALP00000008107; ENSGALG00000005065.
GeneIDi396394.
KEGGigga:396394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10329 mRNA. Translation: AAA53228.1 .
PIRi I50699.
RefSeqi NP_990754.1. NM_205423.1.
UniGenei Gga.3723.

3D structure databases

ProteinModelPortali P49147.
SMRi P49147. Positions 13-721.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000038517.

Proteomic databases

PaxDbi P49147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000008121 ; ENSGALP00000008107 ; ENSGALG00000005065 .
GeneIDi 396394.
KEGGi gga:396394.

Organism-specific databases

CTDi 5321.

Phylogenomic databases

eggNOGi NOG257248.
GeneTreei ENSGT00550000074489.
HOGENOMi HOG000115420.
HOVERGENi HBG053479.
InParanoidi P49147.
KOi K16342.
OrthoDBi EOG73V6JM.
PhylomeDBi P49147.

Enzyme and pathway databases

Reactomei REACT_194344. Synthesis of PA.
REACT_194362. Arachidonic acid metabolism.
REACT_197168. Acyl chain remodeling of CL.
REACT_197169. Acyl chain remodelling of PE.
REACT_197170. Acyl chain remodelling of PS.
REACT_197172. Hydrolysis of LPC.
REACT_197175. Acyl chain remodelling of PG.
REACT_197176. Acyl chain remodelling of PC.
REACT_197223. Acyl chain remodelling of PI.
REACT_236020. Platelet sensitization by LDL.
REACT_241087. ADP signalling through P2Y purinoceptor 1.
REACT_253568. phospho-PLA2 pathway.

Miscellaneous databases

NextBioi 20816436.
PROi P49147.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Delineation of two functionally distinct domains of cytosolic phospholipase A2, a regulatory Ca(2+)-dependent lipid-binding domain and a Ca(2+)-independent catalytic domain."
    Nalefski E.A., Sultzman L.A., Martin D.M., Kriz R.W., Towler P.S., Knopf J.L., Clark J.D.
    J. Biol. Chem. 269:18239-18249(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Entry informationi

Entry nameiPA24A_CHICK
AccessioniPrimary (citable) accession number: P49147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3