P49146 (NPY2R_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuropeptide Y receptor type 2
Short name=NPY2-R

Alternative name(s):
NPY-Y2 receptor
Short name=Y2 receptor

Gene names

Name:

NPY2R

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu-31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	High levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Traceable author statement Ref.3. Source: ProtInc cardiac left ventricle morphogenesis Inferred from mutant phenotype PubMed 19593212. Source: BHF-UCL locomotory behavior Traceable author statement PubMed 9692752. Source: ProtInc outflow tract morphogenesis Inferred from mutant phenotype PubMed 19593212. Source: BHF-UCL
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	calcium channel regulator activity Traceable author statement PubMed 8132547. Source: ProtInc neuropeptide Y receptor activity Traceable author statement Ref.3. Source: ProtInc peptide YY receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Neuropeptide Y receptor type 2		PRO_0000069928
Regions
	Topological domain	1 – 51	51	Extracellular Potential
	Transmembrane	52 – 72	21	Helical; Name=1; Potential
	Topological domain	73 – 86	14	Cytoplasmic Potential
	Transmembrane	87 – 107	21	Helical; Name=2; Potential
	Topological domain	108 – 124	17	Extracellular Potential
	Transmembrane	125 – 145	21	Helical; Name=3; Potential
	Topological domain	146 – 165	20	Cytoplasmic Potential
	Transmembrane	166 – 186	21	Helical; Name=4; Potential
	Topological domain	187 – 216	30	Extracellular Potential
	Transmembrane	217 – 237	21	Helical; Name=5; Potential
	Topological domain	238 – 268	31	Cytoplasmic Potential
	Transmembrane	269 – 289	21	Helical; Name=6; Potential
	Topological domain	290 – 304	15	Extracellular Potential
	Transmembrane	305 – 325	21	Helical; Name=7; Potential
	Topological domain	326 – 381	56	Cytoplasmic Potential
Amino acid modifications
	Lipidation	342	1	S-palmitoyl cysteine Potential
	Glycosylation	11	1	N-linked (GlcNAc...) Potential
	Disulfide bond	123 ↔ 203		By similarity
Experimental info
	Sequence conflict	134	1	V → A in AAA93170. Ref.3
	Sequence conflict	172	1	A → G Ref.4
	Sequence conflict	174	1	G → R Ref.4
	Sequence conflict	202	1	A → P in AAB07760. Ref.4
	Sequence conflict	209	1	G → A in AAB07760. Ref.4
	Sequence conflict	248	1	N → S in AAD00248. Ref.6
	Sequence conflict	311	1	H → Y in AAD00248. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P49146 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7D018C0169597BC7	FASTA	381	42,731
	10 20 30 40 50 60 MGPIGAEADE NQTVEEMKVE QYGPQTTPRG ELVPDPEPEL IDSTKLIEVQ VVLILAYCSI 70 80 90 100 110 120 ILLGVIGNSL VIHVVIKFKS MRTVTNFFIA NLAVADLLVN TLCLPFTLTY TLMGEWKMGP 130 140 150 160 170 180 VLCHLVPYAQ GLAVQVSTIT LTVIALDRHR CIVYHLESKI SKRISFLIIG LAWGISALLA 190 200 210 220 230 240 SPLAIFREYS LIEIIPDFEI VACTEKWPGE EKSIYGTVYS LSSLLILYVL PLGIISFSYT 250 260 270 280 290 300 RIWSKLKNHV SPGAANDHYH QRRQKTTKML VCVVVVFAVS WLPLHAFQLA VDIDSQVLDL 310 320 330 340 350 360 KEYKLIFTVF HIIAMCSTFA NPLLYGWMNS NYRKAFLSAF RCEQRLDAIH SEVSVTFKAK 370 380 KNLEVRKNSG PNDSFTEATN V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning and pharmacological characterization of a human hippocampal neuropeptide Y/peptide YY Y2 receptor subtype." Gerald C., Walker M.W., Vaysse P.J.-J., He C., Branchek T.A., Weinshank R.L. J. Biol. Chem. 270:26758-26761(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hippocampus.
[2]	"Expression cloning of a human brain neuropeptide Y Y2 receptor." Gehlert D.R., Beavers L.S., Johnson D., Gackenheimer S.L., Schober D.A., Gadski R.A. Mol. Pharmacol. 49:224-228(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Cloning and functional expression of a cDNA encoding a human type 2 neuropeptide Y receptor." Rose P.M., Fernandes P., Lynch J.S., Frazier S.T., Fisher S.M., Kodukula K., Kienzle B., Seethala R. J. Biol. Chem. 270:22661-22664(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors." Yan H., Yang J., Marasco J., Yamaguchi K., Brenner S., Collins F., Karbon W. Proc. Natl. Acad. Sci. U.S.A. 93:4661-4665(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Characterization of the human type 2 neuropeptide Y receptor gene (NPY2R) and localization to the chromosome 4q region containing the type 1 neuropeptide Y receptor gene." Ammar D.A., Eadie D.M., Wong D.J., Ma Y.-Y., Kolakowski L.F. Jr., Yang-Feng T.L., Thompson D.A. Genomics 38:392-398(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Human neuropeptide Y Y2 receptor gene." Zastawny R.L. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U36269 mRNA. Translation: AAC50281.1.
U42766 mRNA. Translation: AAB04120.1.
U32500 mRNA. Translation: AAA93170.1.
U42389 mRNA. Translation: AAB07760.1.
U50146 Genomic DNA. Translation: AAC51115.1.
U76254 Genomic DNA. Translation: AAD00248.1.
AY236540 Genomic DNA. Translation: AAO92062.1.
AC104407 Genomic DNA. Translation: AAY40940.1.
CH471056 Genomic DNA. Translation: EAX04901.1.
BC075053 mRNA. Translation: AAH75053.2.
BC075052 mRNA. Translation: AAH75052.2.

IPI

IPI00026065.

PIR

I39187.

RefSeq

NP_000901.1. NM_000910.2.

UniGene

Hs.37125.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IK3	model	-	@	1-381	[»]

ProteinModelPortal

P49146.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000332591.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P49146.

Polymorphism databases

DMDM

1352610.

Proteomic databases

PaxDb

P49146.

PRIDE

P49146.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000329476; ENSP00000332591; ENSG00000185149.
ENST00000506608; ENSP00000426366; ENSG00000185149.

GeneID

4887.

KEGG

hsa:4887.

UCSC

uc003ioq.3. human.

Organism-specific databases

CTD

4887.

GeneCards

GC04P156129.

HGNC

HGNC:7957. NPY2R.

HPA

HPA013965.

MIM

162642. gene.

neXtProt

NX_P49146.

PharmGKB

PA31741.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG267229.

HOGENOM

HOG000050229.

HOVERGEN

HBG105675.

InParanoid

P49146.

KO

K04205.

OMA

CEQRLDS.

OrthoDB

EOG4JT05M.

PhylomeDB

P49146.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P49146.

CleanEx

HS_NPY2R.

Genevestigator

P49146.

GermOnline

ENSG00000185149. Homo sapiens.

Family and domain databases

InterPro

IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001358. NPY2_rcpt.
IPR000611. NPY_rcpt.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00237. GPCRRHODOPSN.
PR01014. NRPEPTIDEY2R.
PR01012. NRPEPTIDEYR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P49146.

ChEMBL

CHEMBL4018.

GenomeRNAi

4887.

NextBio

18814.

SOURCE

Search...

Entry information

Entry name

NPY2R_HUMAN

Accession

Primary (citable) accession number: P49146
Secondary accession number(s): Q13281 , Q13457, Q4W5G7, Q6AZZ6, Q9UE67

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.