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Protein

TFIIH basal transcription factor complex helicase XPB subunit

Gene

Ercc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3488ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. 3'-5' DNA helicase activity Source: UniProtKB
  2. ATPase activity Source: MGI
  3. ATP binding Source: UniProtKB-KW
  4. ATP-dependent DNA helicase activity Source: InterPro
  5. dATP binding Source: Ensembl
  6. DNA binding Source: UniProtKB-KW
  7. GTP binding Source: Ensembl
  8. peptide binding Source: Ensembl
  9. protein C-terminus binding Source: MGI
  10. protein N-terminus binding Source: MGI
  11. RNA polymerase II carboxy-terminal domain kinase activity Source: Ensembl

GO - Biological processi

  1. apoptotic process Source: UniProtKB
  2. ATP catabolic process Source: Ensembl
  3. DNA repair Source: MGI
  4. DNA topological change Source: UniProtKB
  5. hair cell differentiation Source: MGI
  6. nucleotide-excision repair Source: MGI
  7. nucleotide-excision repair, DNA duplex unwinding Source: MGI
  8. nucleotide-excision repair, DNA incision Source: MGI
  9. positive regulation of apoptotic process Source: MGI
  10. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  11. protein localization Source: MGI
  12. regulation of mitotic cell cycle phase transition Source: MGI
  13. response to hypoxia Source: Ensembl
  14. response to oxidative stress Source: MGI
  15. response to UV Source: MGI
  16. transcription-coupled nucleotide-excision repair Source: UniProtKB
  17. transcription from RNA polymerase II promoter Source: UniProtKB
  18. UV protection Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203462. Formation of the Early Elongation Complex.
REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_224562. Dual incision reaction in TC-NER.
REACT_226490. RNA Polymerase II Transcription Elongation.
REACT_230204. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_232235. RNA Polymerase I Promoter Escape.
REACT_232708. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_235780. RNA Polymerase I Transcription Initiation.
REACT_240507. Dual incision reaction in GG-NER.
REACT_244380. RNA Polymerase II Transcription Initiation.
REACT_252106. Formation of incision complex in GG-NER.
REACT_255009. RNA Polymerase II Pre-transcription Events.
REACT_255334. RNA Polymerase II Promoter Escape.
REACT_257165. RNA Polymerase I Transcription Termination.
REACT_257596. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_258231. mRNA Capping.
REACT_259097. RNA Polymerase I Chain Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
TFIIH basal transcription factor complex helicase XPB subunit (EC:3.6.4.12)
Alternative name(s):
Basic transcription factor 2 89 kDa subunit
Short name:
BTF2 p89
DNA excision repair protein ERCC-3
DNA repair protein complementing XP-B cells
TFIIH 89 kDa subunit
Xeroderma pigmentosum group B-complementing protein
Gene namesi
Name:Ercc3
Synonyms:Xpb, Xpbc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:95414. Ercc3.

Subcellular locationi

GO - Cellular componenti

  1. core TFIIH complex Source: Ensembl
  2. holo TFIIH complex Source: UniProtKB
  3. nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783TFIIH basal transcription factor complex helicase XPB subunitPRO_0000101989Add
BLAST

Proteomic databases

MaxQBiP49135.
PaxDbiP49135.
PRIDEiP49135.

PTM databases

PhosphoSiteiP49135.

Expressioni

Gene expression databases

BgeeiP49135.
ExpressionAtlasiP49135. baseline and differential.
GenevestigatoriP49135.

Interactioni

Subunit structurei

One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60 (By similarity). Interacts with ATF7IP (By similarity).By similarity

Protein-protein interaction databases

BioGridi199501. 6 interactions.
IntActiP49135. 1 interaction.
MINTiMINT-4094657.

Structurei

3D structure databases

ProteinModelPortaliP49135.
SMRiP49135. Positions 284-731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini328 – 489162Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini543 – 703161Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi6 – 1813Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi442 – 4454DEVH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi20 – 289Asp/Glu-rich (acidic)
Compositional biasi257 – 26610Asp/Glu-rich (acidic)
Compositional biasi698 – 7014Asp/Glu-rich (acidic)
Compositional biasi722 – 7298Asp/Glu-rich (acidic)

Sequence similaritiesi

Belongs to the helicase family. RAD25/XPB subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1061.
GeneTreeiENSGT00390000002204.
HOGENOMiHOG000160172.
HOVERGENiHBG051499.
InParanoidiP49135.
KOiK10843.
OMAiKYHERRN.
OrthoDBiEOG7DNNTM.
PhylomeDBiP49135.
TreeFamiTF101233.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR001161. Helicase_Ercc3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
PRINTSiPR00851. XRODRMPGMNTB.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00603. rad25. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P49135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRDRVDRD KKKSKKRQYE EEEEDEDDIP GNESQEAVPS AAGKQVDESS
60 70 80 90 100
TKVDEYGAKD YRQQMPLKGD HTSRPLWVAP DGHIFLEAFS PVYKYAQDFL
110 120 130 140 150
VAIAEPVCRP THVHEYKLTA YSLYAAVSVG LQTSDITEYL RKLSKTGVPD
160 170 180 190 200
GIIQFIKLCT VSYGKVKLVL KHNRYFVESS HPDVIQHLLQ DPVIRECRLR
210 220 230 240 250
NAEGEATELI TETFTSKSAI SKTAAEGSGG PSTSQGVDAQ ATSDIPKDLF
260 270 280 290 300
DFYEQMDKDE EEEEETQTVS FEVKQEMIEE LQKRCICLEY PLLAEYDFRN
310 320 330 340 350
DTLNPDINID LKPTAVLRPY QEKSLRKMFG NGRARSGVIV LPCGAGKSLV
360 370 380 390 400
GVTAACTVRK RCLVLGNSAV SVEQWKAQFK MWSTIDDSQI CRFTSDAKDK
410 420 430 440 450
PIGCSVAIST YSMLGHTTKR SWEAERVMEW LKTQEWGLMI LDEVHTIPAR
460 470 480 490 500
MFRRVLTIVQ AHCKLGLTAT LVREDDKIVD LNFLIGPKLY EANWMELQNN
510 520 530 540 550
GYIAKVQCAE VWCPMSPEFY REYVAIKTKK RILLYTMNPN KFRACQFLIK
560 570 580 590 600
FHERRNDKII VFADNVFALK EYAIRLNKPY IYGPTSQGER MQILQNFKHN
610 620 630 640 650
PKINTIFISK VGDTSFDLPE ANVLIQISSH GGSRRQEAQR LGRVLRAKKG
660 670 680 690 700
MVAEEYNAFF YSLVSQDTQE MAYSTKRQRF LVDQGYSFKV ITKLAGMEEE
710 720 730 740 750
ELAFSTKEEQ QQLLQKVLAA TDLDAEEEVV AGEFGSRSGQ ASRRCGTMSS
760 770 780
LSGADDTVYM EYHSSRSKAS SKHVHPLFKR FRK
Length:783
Mass (Da):89,126
Last modified:February 1, 1996 - v1
Checksum:i6D204EF6A831D8AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71186 mRNA. Translation: AAB20614.1.
BC016595 mRNA. Translation: AAH16595.1.
BC026575 mRNA. Translation: AAH26575.1.
CCDSiCCDS29118.1.
PIRiA48994.
RefSeqiNP_598419.1. NM_133658.1.
UniGeneiMm.282335.

Genome annotation databases

EnsembliENSMUST00000025241; ENSMUSP00000025241; ENSMUSG00000024382.
GeneIDi13872.
KEGGimmu:13872.
UCSCiuc008eje.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71186 mRNA. Translation: AAB20614.1.
BC016595 mRNA. Translation: AAH16595.1.
BC026575 mRNA. Translation: AAH26575.1.
CCDSiCCDS29118.1.
PIRiA48994.
RefSeqiNP_598419.1. NM_133658.1.
UniGeneiMm.282335.

3D structure databases

ProteinModelPortaliP49135.
SMRiP49135. Positions 284-731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199501. 6 interactions.
IntActiP49135. 1 interaction.
MINTiMINT-4094657.

PTM databases

PhosphoSiteiP49135.

Proteomic databases

MaxQBiP49135.
PaxDbiP49135.
PRIDEiP49135.

Protocols and materials databases

DNASUi13872.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025241; ENSMUSP00000025241; ENSMUSG00000024382.
GeneIDi13872.
KEGGimmu:13872.
UCSCiuc008eje.1. mouse.

Organism-specific databases

CTDi2071.
MGIiMGI:95414. Ercc3.

Phylogenomic databases

eggNOGiCOG1061.
GeneTreeiENSGT00390000002204.
HOGENOMiHOG000160172.
HOVERGENiHBG051499.
InParanoidiP49135.
KOiK10843.
OMAiKYHERRN.
OrthoDBiEOG7DNNTM.
PhylomeDBiP49135.
TreeFamiTF101233.

Enzyme and pathway databases

ReactomeiREACT_203462. Formation of the Early Elongation Complex.
REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_224562. Dual incision reaction in TC-NER.
REACT_226490. RNA Polymerase II Transcription Elongation.
REACT_230204. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_232235. RNA Polymerase I Promoter Escape.
REACT_232708. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_235780. RNA Polymerase I Transcription Initiation.
REACT_240507. Dual incision reaction in GG-NER.
REACT_244380. RNA Polymerase II Transcription Initiation.
REACT_252106. Formation of incision complex in GG-NER.
REACT_255009. RNA Polymerase II Pre-transcription Events.
REACT_255334. RNA Polymerase II Promoter Escape.
REACT_257165. RNA Polymerase I Transcription Termination.
REACT_257596. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_258231. mRNA Capping.
REACT_259097. RNA Polymerase I Chain Elongation.

Miscellaneous databases

NextBioi284792.
PROiP49135.
SOURCEiSearch...

Gene expression databases

BgeeiP49135.
ExpressionAtlasiP49135. baseline and differential.
GenevestigatoriP49135.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR001161. Helicase_Ercc3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
PRINTSiPR00851. XRODRMPGMNTB.
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00603. rad25. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse homolog of the XPBC/ERCC-3 gene implicated in xeroderma pigmentosum and Cockayne's syndrome."
    Weeda G., Ma L., van Ham R.C., Bootsma D., der Eb A.J., Hoeijmakers J.H.
    Carcinogenesis 12:2361-2368(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiERCC3_MOUSE
AccessioniPrimary (citable) accession number: P49135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 4, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.