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Protein

General transcription and DNA repair factor IIH helicase subunit XPB

Gene

Ercc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 348ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-72086 mRNA Capping
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription and DNA repair factor IIH helicase subunit XPB (EC:3.6.4.12)
Short name:
TFIIH subunit XPB
Alternative name(s):
Basic transcription factor 2 89 kDa subunit
Short name:
BTF2 p89
DNA excision repair protein ERCC-3
DNA repair protein complementing XP-B cells
TFIIH 89 kDa subunit
Xeroderma pigmentosum group B-complementing protein
Gene namesi
Name:Ercc3
Synonyms:Xpb, Xpbc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:95414 Ercc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001019891 – 783General transcription and DNA repair factor IIH helicase subunit XPBAdd BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineBy similarity1
Modified residuei687PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP49135
MaxQBiP49135
PaxDbiP49135
PeptideAtlasiP49135
PRIDEiP49135

PTM databases

iPTMnetiP49135
PhosphoSitePlusiP49135

Expressioni

Gene expression databases

BgeeiENSMUSG00000024382
GenevisibleiP49135 MM

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60. Interacts with ATF7IP.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1995016 interactors.
IntActiP49135 1 interactor.
MINTiP49135
STRINGi10090.ENSMUSP00000025241

Structurei

3D structure databases

ProteinModelPortaliP49135
SMRiP49135
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini328 – 489Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST162
Domaini543 – 703Helicase C-terminalPROSITE-ProRule annotationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi6 – 18Nuclear localization signalSequence analysisAdd BLAST13
Motifi442 – 445DEVH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 28Asp/Glu-rich (acidic)9
Compositional biasi257 – 266Asp/Glu-rich (acidic)10
Compositional biasi698 – 701Asp/Glu-rich (acidic)4
Compositional biasi722 – 729Asp/Glu-rich (acidic)8

Sequence similaritiesi

Belongs to the helicase family. RAD25/XPB subfamily.Curated

Phylogenomic databases

eggNOGiKOG1123 Eukaryota
COG1061 LUCA
GeneTreeiENSGT00390000002204
HOGENOMiHOG000160172
HOVERGENiHBG051499
InParanoidiP49135
KOiK10843
OMAiTKHVHPL
OrthoDBiEOG091G0291
PhylomeDBiP49135
TreeFamiTF101233

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR032438 ERCC3_RAD25_C
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR001161 XPB/Ssl2
IPR032830 XPB/Ssl2_N
PfamiView protein in Pfam
PF16203 ERCC3_RAD25_C, 1 hit
PF13625 Helicase_C_3, 1 hit
PF04851 ResIII, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00603 rad25, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P49135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRDRVDRD KKKSKKRQYE EEEEDEDDIP GNESQEAVPS AAGKQVDESS
60 70 80 90 100
TKVDEYGAKD YRQQMPLKGD HTSRPLWVAP DGHIFLEAFS PVYKYAQDFL
110 120 130 140 150
VAIAEPVCRP THVHEYKLTA YSLYAAVSVG LQTSDITEYL RKLSKTGVPD
160 170 180 190 200
GIIQFIKLCT VSYGKVKLVL KHNRYFVESS HPDVIQHLLQ DPVIRECRLR
210 220 230 240 250
NAEGEATELI TETFTSKSAI SKTAAEGSGG PSTSQGVDAQ ATSDIPKDLF
260 270 280 290 300
DFYEQMDKDE EEEEETQTVS FEVKQEMIEE LQKRCICLEY PLLAEYDFRN
310 320 330 340 350
DTLNPDINID LKPTAVLRPY QEKSLRKMFG NGRARSGVIV LPCGAGKSLV
360 370 380 390 400
GVTAACTVRK RCLVLGNSAV SVEQWKAQFK MWSTIDDSQI CRFTSDAKDK
410 420 430 440 450
PIGCSVAIST YSMLGHTTKR SWEAERVMEW LKTQEWGLMI LDEVHTIPAR
460 470 480 490 500
MFRRVLTIVQ AHCKLGLTAT LVREDDKIVD LNFLIGPKLY EANWMELQNN
510 520 530 540 550
GYIAKVQCAE VWCPMSPEFY REYVAIKTKK RILLYTMNPN KFRACQFLIK
560 570 580 590 600
FHERRNDKII VFADNVFALK EYAIRLNKPY IYGPTSQGER MQILQNFKHN
610 620 630 640 650
PKINTIFISK VGDTSFDLPE ANVLIQISSH GGSRRQEAQR LGRVLRAKKG
660 670 680 690 700
MVAEEYNAFF YSLVSQDTQE MAYSTKRQRF LVDQGYSFKV ITKLAGMEEE
710 720 730 740 750
ELAFSTKEEQ QQLLQKVLAA TDLDAEEEVV AGEFGSRSGQ ASRRCGTMSS
760 770 780
LSGADDTVYM EYHSSRSKAS SKHVHPLFKR FRK
Length:783
Mass (Da):89,126
Last modified:February 1, 1996 - v1
Checksum:i6D204EF6A831D8AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71186 mRNA Translation: AAB20614.1
BC016595 mRNA Translation: AAH16595.1
BC026575 mRNA Translation: AAH26575.1
CCDSiCCDS29118.1
PIRiA48994
RefSeqiNP_598419.1, NM_133658.1
UniGeneiMm.282335

Genome annotation databases

EnsembliENSMUST00000025241; ENSMUSP00000025241; ENSMUSG00000024382
GeneIDi13872
KEGGimmu:13872
UCSCiuc008eje.1 mouse

Similar proteinsi

Entry informationi

Entry nameiERCC3_MOUSE
AccessioniPrimary (citable) accession number: P49135
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome