Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

General transcription and DNA repair factor IIH helicase subunit XPB

Gene

Ercc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 348ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-113418. Formation of the Early Elongation Complex.
R-MMU-5696395. Formation of Incision Complex in GG-NER.
R-MMU-5696400. Dual Incision in GG-NER.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6781823. Formation of TC-NER Pre-Incision Complex.
R-MMU-6782135. Dual incision in TC-NER.
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-72086. mRNA Capping.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73776. RNA Polymerase II Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-MMU-73863. RNA Polymerase I Transcription Termination.
R-MMU-75953. RNA Polymerase II Transcription Initiation.
R-MMU-75955. RNA Polymerase II Transcription Elongation.
R-MMU-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-MMU-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription and DNA repair factor IIH helicase subunit XPB (EC:3.6.4.12)
Short name:
TFIIH subunit XPB
Alternative name(s):
Basic transcription factor 2 89 kDa subunit
Short name:
BTF2 p89
DNA excision repair protein ERCC-3
DNA repair protein complementing XP-B cells
TFIIH 89 kDa subunit
Xeroderma pigmentosum group B-complementing protein
Gene namesi
Name:Ercc3
Synonyms:Xpb, Xpbc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:95414. Ercc3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001019891 – 783General transcription and DNA repair factor IIH helicase subunit XPBAdd BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineBy similarity1
Modified residuei687PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP49135.
MaxQBiP49135.
PaxDbiP49135.
PeptideAtlasiP49135.
PRIDEiP49135.

PTM databases

iPTMnetiP49135.
PhosphoSitePlusiP49135.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024382.
GenevisibleiP49135. MM.

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60. Interacts with ATF7IP.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199501. 6 interactors.
IntActiP49135. 1 interactor.
MINTiMINT-4094657.
STRINGi10090.ENSMUSP00000025241.

Structurei

3D structure databases

ProteinModelPortaliP49135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini328 – 489Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST162
Domaini543 – 703Helicase C-terminalPROSITE-ProRule annotationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi6 – 18Nuclear localization signalSequence analysisAdd BLAST13
Motifi442 – 445DEVH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 28Asp/Glu-rich (acidic)9
Compositional biasi257 – 266Asp/Glu-rich (acidic)10
Compositional biasi698 – 701Asp/Glu-rich (acidic)4
Compositional biasi722 – 729Asp/Glu-rich (acidic)8

Sequence similaritiesi

Belongs to the helicase family. RAD25/XPB subfamily.Curated

Phylogenomic databases

eggNOGiKOG1123. Eukaryota.
COG1061. LUCA.
GeneTreeiENSGT00390000002204.
HOGENOMiHOG000160172.
HOVERGENiHBG051499.
InParanoidiP49135.
KOiK10843.
OMAiTKHVHPL.
OrthoDBiEOG091G0291.
PhylomeDBiP49135.
TreeFamiTF101233.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR032438. ERCC3_RAD25_C.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001161. XPB/Ssl2.
IPR032830. XPB/Ssl2_N.
PfamiView protein in Pfam
PF16203. ERCC3_RAD25_C. 1 hit.
PF13625. Helicase_C_3. 1 hit.
PF04851. ResIII. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00603. rad25. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequencei

Sequence statusi: Complete.

P49135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKRDRVDRD KKKSKKRQYE EEEEDEDDIP GNESQEAVPS AAGKQVDESS
60 70 80 90 100
TKVDEYGAKD YRQQMPLKGD HTSRPLWVAP DGHIFLEAFS PVYKYAQDFL
110 120 130 140 150
VAIAEPVCRP THVHEYKLTA YSLYAAVSVG LQTSDITEYL RKLSKTGVPD
160 170 180 190 200
GIIQFIKLCT VSYGKVKLVL KHNRYFVESS HPDVIQHLLQ DPVIRECRLR
210 220 230 240 250
NAEGEATELI TETFTSKSAI SKTAAEGSGG PSTSQGVDAQ ATSDIPKDLF
260 270 280 290 300
DFYEQMDKDE EEEEETQTVS FEVKQEMIEE LQKRCICLEY PLLAEYDFRN
310 320 330 340 350
DTLNPDINID LKPTAVLRPY QEKSLRKMFG NGRARSGVIV LPCGAGKSLV
360 370 380 390 400
GVTAACTVRK RCLVLGNSAV SVEQWKAQFK MWSTIDDSQI CRFTSDAKDK
410 420 430 440 450
PIGCSVAIST YSMLGHTTKR SWEAERVMEW LKTQEWGLMI LDEVHTIPAR
460 470 480 490 500
MFRRVLTIVQ AHCKLGLTAT LVREDDKIVD LNFLIGPKLY EANWMELQNN
510 520 530 540 550
GYIAKVQCAE VWCPMSPEFY REYVAIKTKK RILLYTMNPN KFRACQFLIK
560 570 580 590 600
FHERRNDKII VFADNVFALK EYAIRLNKPY IYGPTSQGER MQILQNFKHN
610 620 630 640 650
PKINTIFISK VGDTSFDLPE ANVLIQISSH GGSRRQEAQR LGRVLRAKKG
660 670 680 690 700
MVAEEYNAFF YSLVSQDTQE MAYSTKRQRF LVDQGYSFKV ITKLAGMEEE
710 720 730 740 750
ELAFSTKEEQ QQLLQKVLAA TDLDAEEEVV AGEFGSRSGQ ASRRCGTMSS
760 770 780
LSGADDTVYM EYHSSRSKAS SKHVHPLFKR FRK
Length:783
Mass (Da):89,126
Last modified:February 1, 1996 - v1
Checksum:i6D204EF6A831D8AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71186 mRNA. Translation: AAB20614.1.
BC016595 mRNA. Translation: AAH16595.1.
BC026575 mRNA. Translation: AAH26575.1.
CCDSiCCDS29118.1.
PIRiA48994.
RefSeqiNP_598419.1. NM_133658.1.
UniGeneiMm.282335.

Genome annotation databases

EnsembliENSMUST00000025241; ENSMUSP00000025241; ENSMUSG00000024382.
GeneIDi13872.
KEGGimmu:13872.
UCSCiuc008eje.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiERCC3_MOUSE
AccessioniPrimary (citable) accession number: P49135
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 31, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families