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P49135

- ERCC3_MOUSE

UniProt

P49135 - ERCC3_MOUSE

Protein

TFIIH basal transcription factor complex helicase XPB subunit

Gene

Ercc3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3488ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 3'-5' DNA helicase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. ATP-dependent DNA helicase activity Source: Ensembl
    4. dATP binding Source: Ensembl
    5. DNA binding Source: UniProtKB-KW
    6. GTP binding Source: Ensembl
    7. peptide binding Source: Ensembl
    8. RNA polymerase II carboxy-terminal domain kinase activity Source: Ensembl

    GO - Biological processi

    1. apoptotic process Source: UniProtKB
    2. DNA topological change Source: UniProtKB
    3. hair cell differentiation Source: Ensembl
    4. nucleotide-excision repair, DNA duplex unwinding Source: Ensembl
    5. nucleotide-excision repair, DNA incision Source: Ensembl
    6. positive regulation of apoptotic process Source: Ensembl
    7. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    8. protein localization Source: Ensembl
    9. regulation of mitotic cell cycle phase transition Source: Ensembl
    10. response to hypoxia Source: Ensembl
    11. response to oxidative stress Source: Ensembl
    12. transcription-coupled nucleotide-excision repair Source: UniProtKB
    13. transcription from RNA polymerase II promoter Source: UniProtKB
    14. UV protection Source: MGI

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_203462. Formation of the Early Elongation Complex.
    REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    TFIIH basal transcription factor complex helicase XPB subunit (EC:3.6.4.12)
    Alternative name(s):
    Basic transcription factor 2 89 kDa subunit
    Short name:
    BTF2 p89
    DNA excision repair protein ERCC-3
    DNA repair protein complementing XP-B cells
    TFIIH 89 kDa subunit
    Xeroderma pigmentosum group B-complementing protein
    Gene namesi
    Name:Ercc3
    Synonyms:Xpb, Xpbc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:95414. Ercc3.

    Subcellular locationi

    GO - Cellular componenti

    1. holo TFIIH complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 783783TFIIH basal transcription factor complex helicase XPB subunitPRO_0000101989Add
    BLAST

    Proteomic databases

    MaxQBiP49135.
    PaxDbiP49135.
    PRIDEiP49135.

    PTM databases

    PhosphoSiteiP49135.

    Expressioni

    Gene expression databases

    BgeeiP49135.
    GenevestigatoriP49135.

    Interactioni

    Subunit structurei

    One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60 By similarity. Interacts with ATF7IP By similarity.By similarity

    Protein-protein interaction databases

    BioGridi199501. 6 interactions.
    IntActiP49135. 1 interaction.
    MINTiMINT-4094657.

    Structurei

    3D structure databases

    ProteinModelPortaliP49135.
    SMRiP49135. Positions 284-731.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini328 – 489162Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini543 – 703161Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi6 – 1813Nuclear localization signalSequence AnalysisAdd
    BLAST
    Motifi442 – 4454DEVH box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi20 – 289Asp/Glu-rich (acidic)
    Compositional biasi257 – 26610Asp/Glu-rich (acidic)
    Compositional biasi698 – 7014Asp/Glu-rich (acidic)
    Compositional biasi722 – 7298Asp/Glu-rich (acidic)

    Sequence similaritiesi

    Belongs to the helicase family. RAD25/XPB subfamily.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1061.
    GeneTreeiENSGT00390000002204.
    HOGENOMiHOG000160172.
    HOVERGENiHBG051499.
    InParanoidiP49135.
    KOiK10843.
    OMAiAPSKHVH.
    OrthoDBiEOG7DNNTM.
    PhylomeDBiP49135.
    TreeFamiTF101233.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR001161. Helicase_Ercc3.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view]
    PRINTSiPR00851. XRODRMPGMNTB.
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    TIGRFAMsiTIGR00603. rad25. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P49135-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKRDRVDRD KKKSKKRQYE EEEEDEDDIP GNESQEAVPS AAGKQVDESS    50
    TKVDEYGAKD YRQQMPLKGD HTSRPLWVAP DGHIFLEAFS PVYKYAQDFL 100
    VAIAEPVCRP THVHEYKLTA YSLYAAVSVG LQTSDITEYL RKLSKTGVPD 150
    GIIQFIKLCT VSYGKVKLVL KHNRYFVESS HPDVIQHLLQ DPVIRECRLR 200
    NAEGEATELI TETFTSKSAI SKTAAEGSGG PSTSQGVDAQ ATSDIPKDLF 250
    DFYEQMDKDE EEEEETQTVS FEVKQEMIEE LQKRCICLEY PLLAEYDFRN 300
    DTLNPDINID LKPTAVLRPY QEKSLRKMFG NGRARSGVIV LPCGAGKSLV 350
    GVTAACTVRK RCLVLGNSAV SVEQWKAQFK MWSTIDDSQI CRFTSDAKDK 400
    PIGCSVAIST YSMLGHTTKR SWEAERVMEW LKTQEWGLMI LDEVHTIPAR 450
    MFRRVLTIVQ AHCKLGLTAT LVREDDKIVD LNFLIGPKLY EANWMELQNN 500
    GYIAKVQCAE VWCPMSPEFY REYVAIKTKK RILLYTMNPN KFRACQFLIK 550
    FHERRNDKII VFADNVFALK EYAIRLNKPY IYGPTSQGER MQILQNFKHN 600
    PKINTIFISK VGDTSFDLPE ANVLIQISSH GGSRRQEAQR LGRVLRAKKG 650
    MVAEEYNAFF YSLVSQDTQE MAYSTKRQRF LVDQGYSFKV ITKLAGMEEE 700
    ELAFSTKEEQ QQLLQKVLAA TDLDAEEEVV AGEFGSRSGQ ASRRCGTMSS 750
    LSGADDTVYM EYHSSRSKAS SKHVHPLFKR FRK 783
    Length:783
    Mass (Da):89,126
    Last modified:February 1, 1996 - v1
    Checksum:i6D204EF6A831D8AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S71186 mRNA. Translation: AAB20614.1.
    BC016595 mRNA. Translation: AAH16595.1.
    BC026575 mRNA. Translation: AAH26575.1.
    CCDSiCCDS29118.1.
    PIRiA48994.
    RefSeqiNP_598419.1. NM_133658.1.
    UniGeneiMm.282335.

    Genome annotation databases

    EnsembliENSMUST00000025241; ENSMUSP00000025241; ENSMUSG00000024382.
    GeneIDi13872.
    KEGGimmu:13872.
    UCSCiuc008eje.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S71186 mRNA. Translation: AAB20614.1 .
    BC016595 mRNA. Translation: AAH16595.1 .
    BC026575 mRNA. Translation: AAH26575.1 .
    CCDSi CCDS29118.1.
    PIRi A48994.
    RefSeqi NP_598419.1. NM_133658.1.
    UniGenei Mm.282335.

    3D structure databases

    ProteinModelPortali P49135.
    SMRi P49135. Positions 284-731.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199501. 6 interactions.
    IntActi P49135. 1 interaction.
    MINTi MINT-4094657.

    PTM databases

    PhosphoSitei P49135.

    Proteomic databases

    MaxQBi P49135.
    PaxDbi P49135.
    PRIDEi P49135.

    Protocols and materials databases

    DNASUi 13872.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025241 ; ENSMUSP00000025241 ; ENSMUSG00000024382 .
    GeneIDi 13872.
    KEGGi mmu:13872.
    UCSCi uc008eje.1. mouse.

    Organism-specific databases

    CTDi 2071.
    MGIi MGI:95414. Ercc3.

    Phylogenomic databases

    eggNOGi COG1061.
    GeneTreei ENSGT00390000002204.
    HOGENOMi HOG000160172.
    HOVERGENi HBG051499.
    InParanoidi P49135.
    KOi K10843.
    OMAi APSKHVH.
    OrthoDBi EOG7DNNTM.
    PhylomeDBi P49135.
    TreeFami TF101233.

    Enzyme and pathway databases

    Reactomei REACT_203462. Formation of the Early Elongation Complex.
    REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Miscellaneous databases

    NextBioi 284792.
    PROi P49135.
    SOURCEi Search...

    Gene expression databases

    Bgeei P49135.
    Genevestigatori P49135.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR001161. Helicase_Ercc3.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00271. Helicase_C. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view ]
    PRINTSi PR00851. XRODRMPGMNTB.
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    TIGRFAMsi TIGR00603. rad25. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the mouse homolog of the XPBC/ERCC-3 gene implicated in xeroderma pigmentosum and Cockayne's syndrome."
      Weeda G., Ma L., van Ham R.C., Bootsma D., der Eb A.J., Hoeijmakers J.H.
      Carcinogenesis 12:2361-2368(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiERCC3_MOUSE
    AccessioniPrimary (citable) accession number: P49135
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3