Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysosome-associated membrane glycoprotein 2

Gene

LAMP2

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live. Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (By similarity). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processAutophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Alternative name(s):
CD107 antigen-like family member B
Lysosomal membrane glycoprotein B
Short name:
LGP-B
CD_antigen: CD107b
Gene namesi
Name:LAMP2
Synonyms:LGPB
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

  • Cell membrane 1 Publication; Single-pass type I membrane protein PROSITE-ProRule annotation
  • Endosome membrane By similarity; Single-pass type I membrane protein PROSITE-ProRule annotation
  • Lysosome membrane PROSITE-ProRule annotation1 Publication; Single-pass type I membrane protein PROSITE-ProRule annotation
  • Cytoplasmic vesicleautophagosome membrane By similarity

  • Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 375LumenalSequence analysisAdd BLAST347
Transmembranei376 – 399HelicalPROSITE-ProRule annotationAdd BLAST24
Topological domaini400 – 410CytoplasmicPROSITE-ProRule annotationAdd BLAST11

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000001711329 – 410Lysosome-associated membrane glycoprotein 2Add BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 79PROSITE-ProRule annotation
Glycosylationi48N-linked (GlcNAc...)Sequence analysis1
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Glycosylationi123N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi153 ↔ 189PROSITE-ProRule annotation
Glycosylationi179N-linked (GlcNAc...)Sequence analysis1
Glycosylationi222N-linked (GlcNAc...)Sequence analysis1
Glycosylationi229N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi232 ↔ 265PROSITE-ProRule annotation
Glycosylationi242N-linked (GlcNAc...)Sequence analysis1
Glycosylationi260N-linked (GlcNAc...)Sequence analysis1
Glycosylationi275N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi317N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi331 ↔ 368PROSITE-ProRule annotation
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Extensively N-glycosylated. Contains a minor proportion of O-linked glycans.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer. Forms large homooligomers. Interacts (via its cytoplasmic region) with HSPA8. Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity). Interacts with MLLT11 (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 192First lumenal domainAdd BLAST164
Regioni193 – 228HingeAdd BLAST36
Regioni229 – 375Second lumenal domainAdd BLAST147
Regioni401 – 404Important for binding and subsequent lysosomal degradation of target proteinsBy similarity4

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG052303.
KOiK06528.

Family and domain databases

InterProiView protein in InterPro
IPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiView protein in Pfam
PF01299. Lamp. 1 hit.
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiView protein in PROSITE
PS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMCFRLSPVS GSGLVLSCLL LGAVQSYAFE LNLPDSKATC LFAKWKMNFT
60 70 80 90 100
ISYETTTNKT LKTVTISEPH NVTYNGSSCG DDQGVAKIAV QFGSTVSWNV
110 120 130 140 150
TFTKEESHYV IGSIWLVYNT SDNTTFPGAI PKGSATVISS QSIEIPLDDI
160 170 180 190 200
FRCNSLLTFK TGNVVQNYWD IHLQAFVQNG TVSKEEFVCE EDKSVTTVRP
210 220 230 240 250
IIHTTVPPPT TTPTPLPPKV GNYSVSNGNA TCLLATMGLQ LNVTEEKVPF
260 270 280 290 300
IFNINPSTTN FTGSCHPQTA QLRLNNSQIK YLDFIFAVKS ESHFYLKEVN
310 320 330 340 350
VSMYMANGSV FSVANNNLSF WDAPLGSSYM CNKEQVVSVS RTFQINTFNL
360 370 380 390 400
KVQPFNVTKG KYATAQDCSA DEDNFLVPIA VGAALAGVLA LVLLAYFIGL
410
KRHHTGYEQF
Length:410
Mass (Da):45,028
Last modified:February 1, 1996 - v1
Checksum:iADA8EBF04285C406
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19357 mRNA. Translation: AAC37683.1.
RefSeqiNP_001233678.1. NM_001246749.1.

Genome annotation databases

GeneIDi100689316.
KEGGicge:100689316.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19357 mRNA. Translation: AAC37683.1.
RefSeqiNP_001233678.1. NM_001246749.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689316.
KEGGicge:100689316.

Organism-specific databases

CTDi3920.

Phylogenomic databases

HOVERGENiHBG052303.
KOiK06528.

Family and domain databases

InterProiView protein in InterPro
IPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiView protein in Pfam
PF01299. Lamp. 1 hit.
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiView protein in PROSITE
PS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLAMP2_CRIGR
AccessioniPrimary (citable) accession number: P49130
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 15, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.