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P49102 (NIA3_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitrate reductase [NADH] 3

Short name=NR
EC=1.7.1.1
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length889 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activity

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactor

Binds 1 FAD per subunit By similarity.

Binds 1 heme group per subunit By similarity.

Binds 1 molybdenum-pterin group per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the nitrate reductase family.

Contains 1 cytochrome b5 heme-binding domain.

Contains 1 FAD-binding FR-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 889889Nitrate reductase [NADH] 3
PRO_0000166064

Regions

Domain520 – 59576Cytochrome b5 heme-binding
Domain630 – 742113FAD-binding FR-type

Sites

Metal binding1811Molybdenum-pterin Potential
Metal binding2351Molybdenum-pterin Potential
Metal binding5551Iron (heme axial ligand) By similarity
Metal binding5781Iron (heme axial ligand) By similarity

Amino acid modifications

Disulfide bond404Interchain Potential

Sequences

Sequence LengthMass (Da)Tools
P49102 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 3614A8BB44B822F5

FASTA88998,811
        10         20         30         40         50         60 
MSTCVEQPTH SASLDPTAAQ RLPYPDLPVD ILRRSSVRGS GFVAAALVSS ARKADDDARH 

        70         80         90        100        110        120 
DDDDPSGDRH ETYGSHYLAN LGVEQSVRDE GTVDAWVESS QSLIRLTGKH SLNGELPRLM 

       130        140        150        160        170        180 
RHGFITPVPR HYVRNHGPVP RGDWATWTVE VTGLVRRPRA LTMDELARDF PALELPVTLV 

       190        200        210        220        230        240 
CAGNRRKEQN MVRQTLGFNW GPGAVSTSVW RGARLSDVLR RCGSMSRKGG ALFVCFEGAE 

       250        260        270        280        290        300 
DLPGGGGTKY GTSITREVAL DPTMDVMLAY QQNGGPLLPD HGFPVRLIVP GCTAGRMVKW 

       310        320        330        340        350        360 
LKRIVVAPAE SDNYYHYRDN RFLPSHVDAK LADAEGWWYK PEYVINEMNT NSVITTPAHN 

       370        380        390        400        410        420 
EFLPINAITT QRIYTMKGFA YSGGGKKVTR VEVTLDGGEN WLLCELDHPE KPTKYGRYWC 

       430        440        450        460        470        480 
WCFWSIDVEL IDLLACKEIA VRAWDQSLNT QPEFLTWNLM GMMTNCWFRV KVNVCRPRHG 

       490        500        510        520        530        540 
EKAGLAFEHP VRTNQPGGWM AQQKHLETAE RTSAATSTTN QQFTMSEVRK HASQDSAWIV 

       550        560        570        580        590        600 
VHGHVYDCTA FLKDHPGGAD SILINAGTDC TEEFDAIHSD KAKELLDTYR IGDLVTTGGA 

       610        620        630        640        650        660 
EQRSPLELAP SPPIRHEGPA APVIALSNPR EKVPCQLVAR TVLSRDVRLF RFALPSSGQV 

       670        680        690        700        710        720 
LGLPVGKHIF VCASIDGKLC MRAYTPTSSV DEVGHFDLLV KVYFRNENTK FPDGGRMTQY 

       730        740        750        760        770        780 
LDSLPVGAHV DVKGPLGHVE YVGRGGFVID GKPRKAGRLA MVAGGSGITP IYQVIQAVLR 

       790        800        810        820        830        840 
DQPEDKTEMH LVYANRTEDD ILLRAELDRW AAEYPERLKV WYVVSQVKRL DEWKYSVGIV 

       850        860        870        880 
TEAVLRDDVP EARDGTLALL CGPPSMIQSP ILPNLEKMKH QLDDSVVSF 

« Hide

References

[1]Campbell W.H., Redinbaugh M.G., Ingemarsson B., Doughtery E.S., Campbell E.R.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. B73.
Tissue: Seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20450 Genomic DNA. Translation: AAA62316.1.
PIRT02240.

3D structure databases

ProteinModelPortalP49102.
SMRP49102. Positions 632-889.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP49102.
MaizeGDB30041.

Family and domain databases

Gene3D2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMSSF55856. Cyt_B5. 1 hit.
SSF81296. Ig_E-set. 1 hit.
SSF56524. Oxidored_molyb. 1 hit.
SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIA3_MAIZE
AccessionPrimary (citable) accession number: P49102
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 6, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families