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Protein

Nitrate reductase [NADH] 3

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi181MolybdenumBy similarity1
Metal bindingi555Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi578Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] 3 (EC:1.7.1.1)
Short name:
NR
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi30041

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660641 – 889Nitrate reductase [NADH] 3Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi404InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Expressioni

Gene expression databases

ExpressionAtlasiP49102 differential

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP49102
SMRiP49102
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini520 – 595Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini630 – 742FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P49102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTCVEQPTH SASLDPTAAQ RLPYPDLPVD ILRRSSVRGS GFVAAALVSS
60 70 80 90 100
ARKADDDARH DDDDPSGDRH ETYGSHYLAN LGVEQSVRDE GTVDAWVESS
110 120 130 140 150
QSLIRLTGKH SLNGELPRLM RHGFITPVPR HYVRNHGPVP RGDWATWTVE
160 170 180 190 200
VTGLVRRPRA LTMDELARDF PALELPVTLV CAGNRRKEQN MVRQTLGFNW
210 220 230 240 250
GPGAVSTSVW RGARLSDVLR RCGSMSRKGG ALFVCFEGAE DLPGGGGTKY
260 270 280 290 300
GTSITREVAL DPTMDVMLAY QQNGGPLLPD HGFPVRLIVP GCTAGRMVKW
310 320 330 340 350
LKRIVVAPAE SDNYYHYRDN RFLPSHVDAK LADAEGWWYK PEYVINEMNT
360 370 380 390 400
NSVITTPAHN EFLPINAITT QRIYTMKGFA YSGGGKKVTR VEVTLDGGEN
410 420 430 440 450
WLLCELDHPE KPTKYGRYWC WCFWSIDVEL IDLLACKEIA VRAWDQSLNT
460 470 480 490 500
QPEFLTWNLM GMMTNCWFRV KVNVCRPRHG EKAGLAFEHP VRTNQPGGWM
510 520 530 540 550
AQQKHLETAE RTSAATSTTN QQFTMSEVRK HASQDSAWIV VHGHVYDCTA
560 570 580 590 600
FLKDHPGGAD SILINAGTDC TEEFDAIHSD KAKELLDTYR IGDLVTTGGA
610 620 630 640 650
EQRSPLELAP SPPIRHEGPA APVIALSNPR EKVPCQLVAR TVLSRDVRLF
660 670 680 690 700
RFALPSSGQV LGLPVGKHIF VCASIDGKLC MRAYTPTSSV DEVGHFDLLV
710 720 730 740 750
KVYFRNENTK FPDGGRMTQY LDSLPVGAHV DVKGPLGHVE YVGRGGFVID
760 770 780 790 800
GKPRKAGRLA MVAGGSGITP IYQVIQAVLR DQPEDKTEMH LVYANRTEDD
810 820 830 840 850
ILLRAELDRW AAEYPERLKV WYVVSQVKRL DEWKYSVGIV TEAVLRDDVP
860 870 880
EARDGTLALL CGPPSMIQSP ILPNLEKMKH QLDDSVVSF
Length:889
Mass (Da):98,811
Last modified:February 1, 1996 - v1
Checksum:i3614A8BB44B822F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20450 Genomic DNA Translation: AAA62316.1
PIRiT02240

Similar proteinsi

Entry informationi

Entry nameiNIA3_MAIZE
AccessioniPrimary (citable) accession number: P49102
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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