P49100 (CYB5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b5

Gene names

Ordered Locus Names:	Os05g0108800, LOC_Os05g01820
ORF Names:	OSJNBa0068N01.16

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	Cytochrome b5		PRO_0000166027
Regions
	Transmembrane	108 – 128	21	Helical; Potential
	Domain	6 – 82	77	Cytochrome b5 heme-binding
Sites
	Metal binding	41	1	Iron (heme axial ligand) By similarity
	Metal binding	65	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	66	1	S → T in CAA53366. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49100 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 5FBBCF0BE8E60FDE	FASTA	137	15,282
	10 20 30 40 50 60 MSNDNKKVYT LEEVAKHNSK DDCWLIIGGK VYNVSKFLED HPGGDDVLLS STGKDATDDF 70 80 90 100 110 120 EDVGHSTTAR AMMDEYYVGD IDTSTIPART KYVPPKQPHY NQDKTPEFII KILQFLVPLA 130 ILGLAVAIRI YTKSESA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tobacco cytochrome b5: cDNA isolation, expression analysis and in vitro protein targeting." Smith M.A., Stobart A.K., Shewry P.R., Napier J.A. Plant Mol. Biol. 25:527-537(1994) [PubMed: 8049375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Callus.
[2]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75670 mRNA. Translation: CAA53366.1. AC079022 Genomic DNA. Translation: AAK73138.1. AC129716 Genomic DNA. Translation: AAU44139.1.
PIR	S46307.
RefSeq	NP_001054434.1. NM_001060969.1.
UniGene	Os.3430.
3D structure databases
ProteinModelPortal	P49100.
ModBase	Search...
Protein-protein interaction databases
STRING	P49100.
Proteomic databases
PRIDE	P49100.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g01820.1; LOC_Os05g01820.1; LOC_Os05g01820.
GeneID	4337583.
KEGG	osa:4337583.
Organism-specific databases
Gramene	P49100.
Phylogenomic databases
OMA	RTKYVPP.
PhylomeDB	P49100.
ProtClustDB	CLSN2692340.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_ORYSJ

Accession

Primary (citable) accession number: P49100
Secondary accession number(s): Q65X19, Q94HK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 20, 2005
Last modified:	September 21, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program