Reviewed, UniProtKB/Swiss-Prot P49100 (CYB5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Cytochrome b5

Gene names

Ordered Locus Names:	Os05g0108800, LOC_Os05g01820
ORF Names:	OSJNBa0068N01.16

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	Cytochrome b5		PRO_0000166027
Regions
	Transmembrane	108 – 128	21	Potential
	Domain	6 – 82	77	Cytochrome b5 heme-binding
Sites
	Metal binding	41	1	Iron (heme axial ligand) By similarity
	Metal binding	65	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	66	1	S → T in CAA53366. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P49100-1 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 5FBBCF0BE8E60FDE	FASTA	137	15,282
	10 20 30 40 50 60 MSNDNKKVYT LEEVAKHNSK DDCWLIIGGK VYNVSKFLED HPGGDDVLLS STGKDATDDF 70 80 90 100 110 120 EDVGHSTTAR AMMDEYYVGD IDTSTIPART KYVPPKQPHY NQDKTPEFII KILQFLVPLA 130 ILGLAVAIRI YTKSESA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tobacco cytochrome b5: cDNA isolation, expression analysis and in vitro protein targeting." Smith M.A., Stobart A.K., Shewry P.R., Napier J.A. Plant Mol. Biol. 25:527-537(1994) [PubMed: 8049375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Callus.
[2]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75670 mRNA. Translation: CAA53366.1. AC079022 Genomic DNA. Translation: AAK73138.1. AC129716 Genomic DNA. Translation: AAU44139.1.
PIR	S46307.
RefSeq	NP_001054434.1.
UniGene	Os.3430
3D structure databases
HSSP	HSSP built from PDB template 1M2I based on UniProtKB P00171.
ModBase	Search...
Genome annotation databases
GeneID	4337583.
KEGG	osa:4337583.
Organism-specific databases
Gramene	P49100.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_ORYSJ

Accession

Primary (citable) accession number: P49100
Secondary accession number(s): Q65X19, Q94HK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)