Reviewed, UniProtKB/Swiss-Prot P49097 (CYB5_CUSRE)

Names and origin

Protein names	Recommended name:     Cytochrome b5
Organism	Cuscuta reflexa (Southern Asian dodder)
Taxonomic identifier	4129 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Convolvulaceae › Cuscuteae › Cuscuta

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	Cytochrome b5		PRO_0000166024
Regions
	Transmembrane	106 – 126	21	Potential
	Domain	4 – 80	77	Cytochrome b5 heme-binding
Sites
	Metal binding	39	1	Iron (heme axial ligand) By similarity
	Metal binding	63	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49097-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CFF21C952981F5DA	FASTA	135	14,745
	10 20 30 40 50 60 MGGSKVYSLA EVSEHSQPND CWLVIGGKVY DVTKFLDDHP GGADVLLSST AKDATDDFED 70 80 90 100 110 120 IGHSSSARAM MDEMCVGDID SSTIPTKTSY TPPKQPLYNQ DKTPQFIIKL LQFLVPLIIL 130 GVAVGIRFYK KQSSD

References

[1]	"The cDNA sequence of cytochrome b5 associated with cytokinin-induced haustoria formation in Cuscuta reflexa." Subramaniam K., Mahadevan S. Gene 149:375-376(1994) [PubMed: 7959021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L22209 mRNA. Translation: AAA62621.1.
PIR	T09946.
3D structure databases
HSSP	HSSP built from PDB template 1EUE based on UniProtKB P04166.
ModBase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_CUSRE

Accession

Primary (citable) accession number: P49097

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)