Reviewed, UniProtKB/Swiss-Prot P49096 (CYB5_MUSDO)

Names and origin

Protein names

Recommended name:
    Cytochrome b5
      Short name=CYTB5

Gene names

Name:

Cyt-b5

Organism

Musca domestica (House fly)

Taxonomic identifier

7370 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Muscoidea › Muscidae › Musca

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 134

134

Cytochrome b5

PRO_0000166017

Regions

Transmembrane

111 – 131

21

Potential

Domain

6 – 82

77

Cytochrome b5 heme-binding

Sites

Metal binding

41

1

Iron (heme axial ligand) By similarity

Metal binding

65

1

Iron (heme axial ligand) By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

134

Helix Strand Turn

Details...

Helix

11 – 15

5

Beta strand

17 – 19

3

Beta strand

22 – 27

6

Beta strand

30 – 33

4

Helix

35 – 37

3

Turn

38 – 40

3

Helix

46 – 49

4

Turn

50 – 53

4

Helix

57 – 63

7

Helix

67 – 73

7

Helix

74 – 76

3

Beta strand

77 – 81

5

Helix

83 – 85

3

Sequences

	Sequence		Length	Mass (Da)
	P49096-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 11A1A23E235EC3AA	FASTA	134	15,401
	10 20 30 40 50 60 MSSEDVKYFT RAEVAKNNTK DKNWFIIHNN VYDVTAFLNE HPGGEEVLIE QAGKDATEHF 70 80 90 100 110 120 EDVGHSSDAR EMMKQYKVGE LVAEERSNVP EKSEPTWNTE QKTEESSMKS WLMPFVLGLV 130 ATLIYKFFFG TKSQ

References

[1]	Guzov V., Feyereisen R. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Rutgers.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L38464 mRNA. Translation: AAA56985.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IBJ	X-ray	1.55	A	1-88	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR001199. Cyt_B5.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]

Gene3D

G3DSA:3.10.120.10. Cyt_B5. 1 hit.

Pfam

PF00173. Cyt-b5. 1 hit.
[Graphical view]

PRINTS

PR00363. CYTOCHROMEB5.

ProDom

PD000612. Cyt_B5. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYB5_MUSDO

Accession

Primary (citable) accession number: P49096

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)