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P49095 (GCSP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine dehydrogenase (decarboxylating), mitochondrial

EC=1.4.4.2
Alternative name(s):
Glycine cleavage system P protein
Glycine decarboxylase
Glycine decarboxylase complex subunit P
Glycine dehydrogenase (aminomethyl-transferring)
Gene names
Name:GCV2
Synonyms:GSD2
Ordered Locus Names:YMR189W
ORF Names:YM9646.01
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1034 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.

Cofactor

Pyridoxal phosphate By similarity.

Subunit structure

Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.

Subcellular location

Mitochondrion By similarity.

Induction

By glycine. Ref.1

Miscellaneous

Present with 12000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GcvP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 1034Glycine dehydrogenase (decarboxylating), mitochondrialPRO_0000010752

Amino acid modifications

Modified residue7731N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P49095 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: F4D52642B0BDA041

FASTA1,034114,451
        10         20         30         40         50         60 
MLRTRVTALL CRATVRSSTN YVSLARTRSF HSQSILLKTA ATDITSTQYS RIFNPDLKNI 

        70         80         90        100        110        120 
DRPLDTFARR HLGPSPSDVK KMLKTMGYSD LNAFIEELVP PNILKRRPLK LEAPSKGFCE 

       130        140        150        160        170        180 
QEMLQHLEKI ANKNHYKVKN FIGKGYYGTI LPPVIQRNLL ESPEWYTSYT PYQPEISQGR 

       190        200        210        220        230        240 
LEALLNFQTV VSDLTGLPVA NASLLDEGTA AGEAMLLSFN ISRKKKLKYV IDKKLHQQTK 

       250        260        270        280        290        300 
SVLHTRAKPF NIEIIEVDCS DIKKAVDVLK NPDVSGCLVQ YPATDGSILP PDSMKQLSDA 

       310        320        330        340        350        360 
LHSHKSLLSV ASDLMALTLL KPPAHYGADI VLGSSQRFGV PMGYGGPHAA FFAVIDKLNR 

       370        380        390        400        410        420 
KIPGRIVGIS KDRLGKTALR LALQTREQHI KRDKATSNIC TAQALLANVA SSYCVYHGPK 

       430        440        450        460        470        480 
GLQNISRRIF SLTSILANAI ENDSCPHELI NKTWFDTLTI KLGNGISSEQ LLDKALKEFN 

       490        500        510        520        530        540 
INLFAVDTTT ISLALDETTT KADVENLLKV FDIENSSQFL SEDYSNSFPR EFQRTDEILR 

       550        560        570        580        590        600 
NEVFHMHHSE TAMLRYLHRL QSRDLSLANS MIPLGSCTMK LNSTVEMMPI TWPQFSNIHP 

       610        620        630        640        650        660 
FQPSNQVQGY KELITSLEKD LCSITGFDGI SLQPNSGAQG EYTGLRVIRS YLESKGENHR 

       670        680        690        700        710        720 
NVCLIPVSAH GTNPASAAMA GLKVVPVNCL QDGSLDLVDL KNKAEQHSKE LAAVMITYPS 

       730        740        750        760        770        780 
TYGLFEPGIQ HAIDIVHSFG GQVYLDGANM NAQVGLTSPG DLGADVCHLN LHKTFSIPHG 

       790        800        810        820        830        840 
GGGPAGAPIC VKSHLIPHLP KHDVVDMITG IGGSKSIDSV SSAPYGNALV LPISYAYIKM 

       850        860        870        880        890        900 
MGNEGLPFSS VIAMLNSNYM MTRLKDHYKI LFVNEMSTLK HCAHEFIVDL REYKAKGVEA 

       910        920        930        940        950        960 
IDVAKRLQDY GFHAPTLAFP VPGTLMIEPT ESENLEELDR FCDAMISIKE EINALVAGQP 

       970        980        990       1000       1010       1020 
KGQILKNAPH SLEDLITSSN WDTRGYTREE AAYPLPFLRY NKFWPTVARL DDTYGDMNLI 

      1030 
CTCPSVEEIA NETE 

« Hide

References

« Hide 'large scale' references
[1]"Specific induction by glycine of the gene for the P-subunit of glycine decarboxylase from Saccharomyces cerevisiae."
Sinclair D.A., Hong S.-P., Dawes I.W.
Mol. Microbiol. 19:611-623(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20641 Genomic DNA. Translation: AAB18933.1.
Z47815 Genomic DNA. Translation: CAA87810.1.
BK006946 Genomic DNA. Translation: DAA10087.1.
PIRS50917.
RefSeqNP_013914.1. NM_001182695.1.

3D structure databases

ProteinModelPortalP49095.
SMRP49095. Positions 58-1026.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35367. 19 interactions.
IntActP49095. 5 interactions.
MINTMINT-659294.
STRING4932.YMR189W.

Proteomic databases

PaxDbP49095.
PeptideAtlasP49095.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR189W; YMR189W; YMR189W.
GeneID855227.
KEGGsce:YMR189W.

Organism-specific databases

CYGDYMR189w.
SGDS000004801. GCV2.

Phylogenomic databases

eggNOGCOG1003.
GeneTreeENSGT00390000017970.
HOGENOMHOG000239369.
KOK00281.
OMAQTMVCDL.
OrthoDBEOG7KWSS4.

Enzyme and pathway databases

BioCycYEAST:YMR189W-MONOMER.

Gene expression databases

GenevestigatorP49095.

Family and domain databases

Gene3D3.40.640.10. 2 hits.
InterProIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF02347. GDC-P. 2 hits.
[Graphical view]
SUPFAMSSF53383. SSF53383. 3 hits.
TIGRFAMsTIGR00461. gcvP. 1 hit.
ProtoNetSearch...

Other

NextBio978759.
PROP49095.

Entry information

Entry nameGCSP_YEAST
AccessionPrimary (citable) accession number: P49095
Secondary accession number(s): D6W013
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 19, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families