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P49095

- GCSP_YEAST

UniProt

P49095 - GCSP_YEAST

Protein

Glycine dehydrogenase (decarboxylating), mitochondrial

Gene

GCV2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.

    Catalytic activityi

    Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    GO - Molecular functioni

    1. glycine dehydrogenase (decarboxylating) activity Source: SGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glycine decarboxylation via glycine cleavage system Source: SGD
    2. one-carbon metabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciYEAST:YMR189W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine dehydrogenase (decarboxylating), mitochondrial (EC:1.4.4.2)
    Alternative name(s):
    Glycine cleavage system P protein
    Glycine decarboxylase
    Glycine decarboxylase complex subunit P
    Glycine dehydrogenase (aminomethyl-transferring)
    Gene namesi
    Name:GCV2
    Synonyms:GSD2
    Ordered Locus Names:YMR189W
    ORF Names:YM9646.01
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR189w.
    SGDiS000004801. GCV2.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. glycine cleavage complex Source: SGD
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1034Glycine dehydrogenase (decarboxylating), mitochondrialPRO_0000010752
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei773 – 7731N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiP49095.
    PaxDbiP49095.
    PeptideAtlasiP49095.

    Expressioni

    Inductioni

    By glycine.1 Publication

    Gene expression databases

    GenevestigatoriP49095.

    Interactioni

    Subunit structurei

    Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.

    Protein-protein interaction databases

    BioGridi35367. 19 interactions.
    IntActiP49095. 5 interactions.
    MINTiMINT-659294.
    STRINGi4932.YMR189W.

    Structurei

    3D structure databases

    ProteinModelPortaliP49095.
    SMRiP49095. Positions 63-1022.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvP family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1003.
    GeneTreeiENSGT00390000017970.
    HOGENOMiHOG000239369.
    KOiK00281.
    OMAiQPANEEQ.
    OrthoDBiEOG7KWSS4.

    Family and domain databases

    Gene3Di3.40.640.10. 2 hits.
    InterProiIPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR003437. GDC_P_homo.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11773. PTHR11773. 1 hit.
    PfamiPF02347. GDC-P. 2 hits.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 3 hits.
    TIGRFAMsiTIGR00461. gcvP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49095-1 [UniParc]FASTAAdd to Basket

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    MLRTRVTALL CRATVRSSTN YVSLARTRSF HSQSILLKTA ATDITSTQYS     50
    RIFNPDLKNI DRPLDTFARR HLGPSPSDVK KMLKTMGYSD LNAFIEELVP 100
    PNILKRRPLK LEAPSKGFCE QEMLQHLEKI ANKNHYKVKN FIGKGYYGTI 150
    LPPVIQRNLL ESPEWYTSYT PYQPEISQGR LEALLNFQTV VSDLTGLPVA 200
    NASLLDEGTA AGEAMLLSFN ISRKKKLKYV IDKKLHQQTK SVLHTRAKPF 250
    NIEIIEVDCS DIKKAVDVLK NPDVSGCLVQ YPATDGSILP PDSMKQLSDA 300
    LHSHKSLLSV ASDLMALTLL KPPAHYGADI VLGSSQRFGV PMGYGGPHAA 350
    FFAVIDKLNR KIPGRIVGIS KDRLGKTALR LALQTREQHI KRDKATSNIC 400
    TAQALLANVA SSYCVYHGPK GLQNISRRIF SLTSILANAI ENDSCPHELI 450
    NKTWFDTLTI KLGNGISSEQ LLDKALKEFN INLFAVDTTT ISLALDETTT 500
    KADVENLLKV FDIENSSQFL SEDYSNSFPR EFQRTDEILR NEVFHMHHSE 550
    TAMLRYLHRL QSRDLSLANS MIPLGSCTMK LNSTVEMMPI TWPQFSNIHP 600
    FQPSNQVQGY KELITSLEKD LCSITGFDGI SLQPNSGAQG EYTGLRVIRS 650
    YLESKGENHR NVCLIPVSAH GTNPASAAMA GLKVVPVNCL QDGSLDLVDL 700
    KNKAEQHSKE LAAVMITYPS TYGLFEPGIQ HAIDIVHSFG GQVYLDGANM 750
    NAQVGLTSPG DLGADVCHLN LHKTFSIPHG GGGPAGAPIC VKSHLIPHLP 800
    KHDVVDMITG IGGSKSIDSV SSAPYGNALV LPISYAYIKM MGNEGLPFSS 850
    VIAMLNSNYM MTRLKDHYKI LFVNEMSTLK HCAHEFIVDL REYKAKGVEA 900
    IDVAKRLQDY GFHAPTLAFP VPGTLMIEPT ESENLEELDR FCDAMISIKE 950
    EINALVAGQP KGQILKNAPH SLEDLITSSN WDTRGYTREE AAYPLPFLRY 1000
    NKFWPTVARL DDTYGDMNLI CTCPSVEEIA NETE 1034
    Length:1,034
    Mass (Da):114,451
    Last modified:February 1, 1996 - v1
    Checksum:iF4D52642B0BDA041
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20641 Genomic DNA. Translation: AAB18933.1.
    Z47815 Genomic DNA. Translation: CAA87810.1.
    BK006946 Genomic DNA. Translation: DAA10087.1.
    PIRiS50917.
    RefSeqiNP_013914.1. NM_001182695.1.

    Genome annotation databases

    EnsemblFungiiYMR189W; YMR189W; YMR189W.
    GeneIDi855227.
    KEGGisce:YMR189W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20641 Genomic DNA. Translation: AAB18933.1 .
    Z47815 Genomic DNA. Translation: CAA87810.1 .
    BK006946 Genomic DNA. Translation: DAA10087.1 .
    PIRi S50917.
    RefSeqi NP_013914.1. NM_001182695.1.

    3D structure databases

    ProteinModelPortali P49095.
    SMRi P49095. Positions 63-1022.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35367. 19 interactions.
    IntActi P49095. 5 interactions.
    MINTi MINT-659294.
    STRINGi 4932.YMR189W.

    Proteomic databases

    MaxQBi P49095.
    PaxDbi P49095.
    PeptideAtlasi P49095.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR189W ; YMR189W ; YMR189W .
    GeneIDi 855227.
    KEGGi sce:YMR189W.

    Organism-specific databases

    CYGDi YMR189w.
    SGDi S000004801. GCV2.

    Phylogenomic databases

    eggNOGi COG1003.
    GeneTreei ENSGT00390000017970.
    HOGENOMi HOG000239369.
    KOi K00281.
    OMAi QPANEEQ.
    OrthoDBi EOG7KWSS4.

    Enzyme and pathway databases

    BioCyci YEAST:YMR189W-MONOMER.

    Miscellaneous databases

    NextBioi 978759.
    PROi P49095.

    Gene expression databases

    Genevestigatori P49095.

    Family and domain databases

    Gene3Di 3.40.640.10. 2 hits.
    InterProi IPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR003437. GDC_P_homo.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view ]
    PANTHERi PTHR11773. PTHR11773. 1 hit.
    Pfami PF02347. GDC-P. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 3 hits.
    TIGRFAMsi TIGR00461. gcvP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Specific induction by glycine of the gene for the P-subunit of glycine decarboxylase from Saccharomyces cerevisiae."
      Sinclair D.A., Hong S.-P., Dawes I.W.
      Mol. Microbiol. 19:611-623(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGCSP_YEAST
    AccessioniPrimary (citable) accession number: P49095
    Secondary accession number(s): D6W013
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 12000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3