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P49094

- ASNS_MAIZE

UniProt

P49094 - ASNS_MAIZE

Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

ASN1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei98 – 981GlutamineBy similarity
    Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
    Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3422ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:ASN1
    Synonyms:AS
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiP49094.
    MaizeGDBi79071.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 586585Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056924Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP49094.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 516324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    KOiK01953.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49094-1 [UniParc]FASTAAdd to Basket

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    MCGILAVLGV VEVSLAKRSR IIELSRRLRH RGPDWSGLHC HEDCYLAHQR    50
    LAIIDPTSGD QPLYNEDKTV VVTVNGEIYN HEELKAKLKT HEFQTGSDCE 100
    VIAHLYEEYG EEFVDMLDGM FSFVLLDTRD KSFIAARDAI GICPLYMGWG 150
    LDGSVWFSSE MKALSDDCER FITFPPGHLY SSKTGGLRRW YNPPWFSETV 200
    PSTPYNALFL REMFEKAVIK RLMTDVPFGV LLSGGLDSSL VASVASRHLN 250
    ETKVDRQWGN KLHTFCIGLK GSPDLKAARE VADYLSTVHH EFHFTVQEGI 300
    DALEEVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG 350
    GYLYFHKAPN KKEFLEETCR KIKALHLYDC LRANKATSAW GVEARVPFLD 400
    KSFISVAMDI DPEWNMIKRD LGRIEKWVMR KAFDDDEHPY LPKHILYRQK 450
    EQFSDGVGYN WIDGLKSFTE QQVTDEMMNN AAQMFPYNTP VNKEAYYYRM 500
    IFERLFPQDS ARETVPWGPS IACSTPAAIE WVEQWKASND PSGRFISSHD 550
    SAATDHTAVS RRWPTAAARP ANGTVNGKDV PVPIAV 586
    Length:586
    Mass (Da):66,578
    Last modified:January 23, 2007 - v2
    Checksum:i72772722DEEFA222
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82849 mRNA. Translation: CAA58052.1.
    PIRiT02978.
    UniGeneiZm.158300.

    Genome annotation databases

    KEGGizma:542435.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82849 mRNA. Translation: CAA58052.1 .
    PIRi T02978.
    UniGenei Zm.158300.

    3D structure databases

    ProteinModelPortali P49094.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.976.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    KEGGi zma:542435.

    Organism-specific databases

    Gramenei P49094.
    MaizeGDBi 79071.

    Phylogenomic databases

    KOi K01953.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Metabolic regulation of asparagine synthetase gene expression in maize (Zea mays L.) root tips."
      Chevalier C., Bourgeois E., Just D., Raymond P.
      Plant J. 9:1-11(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. DEA.
      Tissue: Root meristem.

    Entry informationi

    Entry nameiASNS_MAIZE
    AccessioniPrimary (citable) accession number: P49094
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3