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P49094 (ASNS_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine synthetase [glutamine-hydrolyzing]

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene names
Name:ASN1
Synonyms:AS
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length586 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem By similarity.

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 586585Asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056924

Regions

Domain2 – 185184Glutamine amidotransferase type-2
Domain193 – 516324Asparagine synthetase
Nucleotide binding341 – 3422ATP By similarity
Region50 – 545Glutamine binding By similarity
Region75 – 773Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site981Glutamine By similarity
Binding site2311ATP; via carbonyl oxygen By similarity
Binding site2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3431Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
P49094 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 72772722DEEFA222

FASTA58666,578
        10         20         30         40         50         60 
MCGILAVLGV VEVSLAKRSR IIELSRRLRH RGPDWSGLHC HEDCYLAHQR LAIIDPTSGD 

        70         80         90        100        110        120 
QPLYNEDKTV VVTVNGEIYN HEELKAKLKT HEFQTGSDCE VIAHLYEEYG EEFVDMLDGM 

       130        140        150        160        170        180 
FSFVLLDTRD KSFIAARDAI GICPLYMGWG LDGSVWFSSE MKALSDDCER FITFPPGHLY 

       190        200        210        220        230        240 
SSKTGGLRRW YNPPWFSETV PSTPYNALFL REMFEKAVIK RLMTDVPFGV LLSGGLDSSL 

       250        260        270        280        290        300 
VASVASRHLN ETKVDRQWGN KLHTFCIGLK GSPDLKAARE VADYLSTVHH EFHFTVQEGI 

       310        320        330        340        350        360 
DALEEVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG GYLYFHKAPN 

       370        380        390        400        410        420 
KKEFLEETCR KIKALHLYDC LRANKATSAW GVEARVPFLD KSFISVAMDI DPEWNMIKRD 

       430        440        450        460        470        480 
LGRIEKWVMR KAFDDDEHPY LPKHILYRQK EQFSDGVGYN WIDGLKSFTE QQVTDEMMNN 

       490        500        510        520        530        540 
AAQMFPYNTP VNKEAYYYRM IFERLFPQDS ARETVPWGPS IACSTPAAIE WVEQWKASND 

       550        560        570        580 
PSGRFISSHD SAATDHTAVS RRWPTAAARP ANGTVNGKDV PVPIAV 

« Hide

References

[1]"Metabolic regulation of asparagine synthetase gene expression in maize (Zea mays L.) root tips."
Chevalier C., Bourgeois E., Just D., Raymond P.
Plant J. 9:1-11(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. DEA.
Tissue: Root meristem.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82849 mRNA. Translation: CAA58052.1.
PIRT02978.
UniGeneZm.158300.

3D structure databases

ProteinModelPortalP49094.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.976.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

KEGGzma:542435.

Organism-specific databases

GrameneP49094.
MaizeGDB79071.

Phylogenomic databases

KOK01953.

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNS_MAIZE
AccessionPrimary (citable) accession number: P49094
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways