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P49093

- ASNS2_LOTJA

UniProt

P49093 - ASNS2_LOTJA

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Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

AS2

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei98 – 981GlutamineBy similarity
Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 2
Gene namesi
Name:AS2
OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifieri34305 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 586585Asparagine synthetase [glutamine-hydrolyzing] 2PRO_0000056923Add
BLAST

Proteomic databases

PRIDEiP49093.

Structurei

3D structure databases

ProteinModelPortaliP49093.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini193 – 516324Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49093 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLHQ HGDNFLAHQR
60 70 80 90 100
LAIVDPASGD QPLFNEDQSI IVTVNGEIFN HEELRKQLPN HKFRTGCDCD
110 120 130 140 150
VIAHLYEEHG ENFVDMLDGI FSFVLLDTRD NSFLVARDAI GVTSLYIGYG
160 170 180 190 200
LDGSVWIASE LKGLNDDCEH FELFPPGHLY SSKEKEFRRW YNPPWFSEAI
210 220 230 240 250
PSAPYDPLAL RQAFEKAIIK RLMTDVPFGV LLSGGLDSSL VASVTARYLA
260 270 280 290 300
DTKAAKQWGS KLHSFCVGLE GAPDLKAARE VADYIGTVHH EFQYTIQDGI
310 320 330 340 350
DAIEDVIYHV ETYDVTTIRA GTPMFLMSRK IKSLGVKMVL SGEGSDEIFA
360 370 380 390 400
GYLYFHKAPN KEELHQETCS KIKALHKYDC LRANKSTYAW GLEARVPFLD
410 420 430 440 450
KKFIDVAMGI DPENKMIKRD EGRIEKWVLR KAFDDEENPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYG WIDGLKDHAA KHVTDKMMLN ASNIYPFNTP NTKEAYYYRM
510 520 530 540 550
IFERFFPQNS ARLSVPGGAS IACSTEKAIE WDAAWSNNLD PSGRAALGVH
560 570 580
DSAYDDQLNK SVSKGVEPEK IIPKMEVSPL GVAILS
Length:586
Mass (Da):65,970
Last modified:January 23, 2007 - v2
Checksum:i8E460FFB526BEF0D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89410 mRNA. Translation: CAA61590.1.
PIRiS69183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89410 mRNA. Translation: CAA61590.1 .
PIRi S69183.

3D structure databases

ProteinModelPortali P49093.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

PRIDEi P49093.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterisation of asparagine synthetase from Lotus japonicus: dynamics of asparagine synthesis in N-sufficient conditions."
    Waterhouse R.N., Smyth A.J., Massoneau A., Prosser I.M., Clarkson D.T.
    Plant Mol. Biol. 30:883-897(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Gifu / B-129.

Entry informationi

Entry nameiASNS2_LOTJA
AccessioniPrimary (citable) accession number: P49093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3