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P49093

- ASNS2_LOTJA

UniProt

P49093 - ASNS2_LOTJA

Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

AS2

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei98 – 981GlutamineBy similarity
    Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
    Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3422ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase 2
    Gene namesi
    Name:AS2
    OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
    Taxonomic identifieri34305 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 586585Asparagine synthetase [glutamine-hydrolyzing] 2PRO_0000056923Add
    BLAST

    Proteomic databases

    PRIDEiP49093.

    Structurei

    3D structure databases

    ProteinModelPortaliP49093.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 516324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49093-1 [UniParc]FASTAAdd to Basket

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    MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLHQ HGDNFLAHQR    50
    LAIVDPASGD QPLFNEDQSI IVTVNGEIFN HEELRKQLPN HKFRTGCDCD 100
    VIAHLYEEHG ENFVDMLDGI FSFVLLDTRD NSFLVARDAI GVTSLYIGYG 150
    LDGSVWIASE LKGLNDDCEH FELFPPGHLY SSKEKEFRRW YNPPWFSEAI 200
    PSAPYDPLAL RQAFEKAIIK RLMTDVPFGV LLSGGLDSSL VASVTARYLA 250
    DTKAAKQWGS KLHSFCVGLE GAPDLKAARE VADYIGTVHH EFQYTIQDGI 300
    DAIEDVIYHV ETYDVTTIRA GTPMFLMSRK IKSLGVKMVL SGEGSDEIFA 350
    GYLYFHKAPN KEELHQETCS KIKALHKYDC LRANKSTYAW GLEARVPFLD 400
    KKFIDVAMGI DPENKMIKRD EGRIEKWVLR KAFDDEENPY LPKHILYRQK 450
    EQFSDGVGYG WIDGLKDHAA KHVTDKMMLN ASNIYPFNTP NTKEAYYYRM 500
    IFERFFPQNS ARLSVPGGAS IACSTEKAIE WDAAWSNNLD PSGRAALGVH 550
    DSAYDDQLNK SVSKGVEPEK IIPKMEVSPL GVAILS 586
    Length:586
    Mass (Da):65,970
    Last modified:January 23, 2007 - v2
    Checksum:i8E460FFB526BEF0D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89410 mRNA. Translation: CAA61590.1.
    PIRiS69183.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89410 mRNA. Translation: CAA61590.1 .
    PIRi S69183.

    3D structure databases

    ProteinModelPortali P49093.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.976.

    Proteomic databases

    PRIDEi P49093.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterisation of asparagine synthetase from Lotus japonicus: dynamics of asparagine synthesis in N-sufficient conditions."
      Waterhouse R.N., Smyth A.J., Massoneau A., Prosser I.M., Clarkson D.T.
      Plant Mol. Biol. 30:883-897(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Gifu / B-129.

    Entry informationi

    Entry nameiASNS2_LOTJA
    AccessioniPrimary (citable) accession number: P49093
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3