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P49092

- ASNS1_LOTJA

UniProt

P49092 - ASNS1_LOTJA

Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

AS1

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei98 – 981GlutamineBy similarity
    Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
    Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3422ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase 1
    Gene namesi
    Name:AS1
    OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
    Taxonomic identifieri34305 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 586585Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000056922Add
    BLAST

    Proteomic databases

    ProMEXiP49092.

    Structurei

    3D structure databases

    ProteinModelPortaliP49092.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 516324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49092-1 [UniParc]FASTAAdd to Basket

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    MCGILAVLGC SDFTQAKRVR VLELSRRLKH RGPDWSGLHQ HGDCYLAHQR    50
    LAIVDPASGD QPLFNEDKSI IVTVNGEIYN HEELRKQLPN HQFRTGSDCD 100
    VIAHLYEEHG ENFMDMLDGI FSFVLLDTRD NTFIVARDAI GVTSLYIGWG 150
    LDGSVWISSE MKGLNDDCEH FEVFPPGHLY SSRERAFRRW YNPTWFSESI 200
    PSAPYDPLAV RHAFEKAVIK RLMTDVPFGV LLSGGLDSSL VASITSRYLA 250
    TTKAAEQWGS KLHSFCVGLE GSPDLKAAKE VADYLGTVHH EFTFTVQDGI 300
    DAIEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKWVI SGEGSDEIFG 350
    GYLYFHKAPN KEEFHTETCR KIKALHQYDC LRANKSTFAW GLEARVPFLD 400
    KEFINVAMNI DPEYKMIKRD EGRIEKYILR RAFDDEEKPY LPKHILYRQK 450
    EQFSDGVGYS WIDGLKDHAA KHVTDKMILN AGNIFRHNTP LTKEAYYYRM 500
    IFERFFPQNS ARLTVPGGPT VACSTAKAVE WDAAWSNNLD PSGRAALGVH 550
    LSAYDDKQNN LINNKPVEFE KLIPMEAPSL GVAIHS 586
    Length:586
    Mass (Da):66,461
    Last modified:January 23, 2007 - v2
    Checksum:iAD32149B6A70CEEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89409 mRNA. Translation: CAA61589.1.
    PIRiS69182.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89409 mRNA. Translation: CAA61589.1 .
    PIRi S69182.

    3D structure databases

    ProteinModelPortali P49092.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.976.

    Proteomic databases

    ProMEXi P49092.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterisation of asparagine synthetase from Lotus japonicus: dynamics of asparagine synthesis in N-sufficient conditions."
      Waterhouse R.N., Smyth A.J., Massoneau A., Prosser I.M., Clarkson D.T.
      Plant Mol. Biol. 30:883-897(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Gifu / B-129.

    Entry informationi

    Entry nameiASNS1_LOTJA
    AccessioniPrimary (citable) accession number: P49092
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3