Reviewed, UniProtKB/Swiss-Prot P49091 (ASNS_BRAOL)

Names and origin

Protein names	Recommended name:     Asparagine synthetase [glutamine-hydrolyzing]     EC=6.3.5.4 Alternative name(s):     Glutamine-dependent asparagine synthetase
Organism	Brassica oleracea (Wild cabbage)
Taxonomic identifier	3712 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Sequence similarities	Belongs to the asparagine synthetase family. Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 586	585	Asparagine synthetase [glutamine-hydrolyzing]		PRO_0000056921
Regions
	Domain	2 – 185	184	Glutamine amidotransferase type-2
	Domain	194 – 517	324	Asparagine synthetase
Sites
	Active site	2	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49091-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: DC58CDE0DF3EF4BA	FASTA	586	65,673
	10 20 30 40 50 60 MCGILAVLGC SDDSQAKRVR VLELSRRLRH RGPDWSGIYQ NGFNYLAHQR LAIIDPDSGD 70 80 90 100 110 120 QPLFNEDKSI VVTVNGEIYN HEELRKGLKN HKFHTGSDCD VIAHLYEEHG ENFVDMLDGI 130 140 150 160 170 180 FSFVLLDTRD NSFMVARDAV GVTSLYIGWG LDGSLWVSSE MKGLHEDCEH FEAFPPGHLY 190 200 210 220 230 240 SSKSGGGFKQ WYNPPWFNES VPSTPYEPLA IRSAFEDAVI KRLMTDVPFG VLLSGGLDSS 250 260 270 280 290 300 LVASITARHL AGTKAAKRWG PQLHSFCVGL EGSPDLKAGK EVAEYLGTVH HEFHFTVQDG 310 320 330 340 350 360 IDAIEDVIYH VETYDVTTIR ASTPMFLMSR KIKSLGVKMV LSGEGSDEIF GGYLYFHKAP 370 380 390 400 410 420 NKQEFHQETC RKIKALHKYD CLRANKATSA FGLEARVPFL DKEFINTAMS LDPESKMIKP 430 440 450 460 470 480 EEGRIEKWVL RRAFDDEERP YLPKHILYRQ KEQFSDGVGY SWIDGLKAHA AENVNDKMMS 490 500 510 520 530 540 KAAFIFPHNT PLTKEAYYYR MIFERFFPQN SARLTVPGGA TVACSTAKAV EWDASWSNNM 550 560 570 580 DPSGRAAIGV HLSAYDGSKV ALPLPAPHKA IDDIPMMMGQ EVVIQT

References

[1]	"An asparagine synthetase cDNA clone from Broccoli (Brassica oleracea L.)." Downs C.G., Pogson B.J., Davies K.M., Almira E.C. Plant Gene Register PGR95-016 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Shogun.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X84448 mRNA. Translation: CAA59138.1.
PIR	S52387.
3D structure databases
HSSP	HSSP built from PDB template 1CT9 based on UniProtKB P22106.
ModBase	Search...
Protein family/group databases
MEROPS	C44.974.
Enzyme and pathway databases
BRENDA	6.3.5.4. 1738.
Family and domain databases
InterPro	IPR006426. Asn_synth_AEB. IPR001962. Asn_synthase. IPR000583. GATase_2. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. PF00310. GATase_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01536. asn_synth_AEB. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNS_BRAOL

Accession

Primary (citable) accession number: P49091

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)