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P49091

- ASNS_BRAOL

UniProt

P49091 - ASNS_BRAOL

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Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene
N/A
Organism
Brassica oleracea (Wild cabbage)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei98 – 981GlutamineBy similarity
Binding sitei232 – 2321ATP; via carbonyl oxygenBy similarity
Binding sitei268 – 2681ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei344 – 3441Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi342 – 3432ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
OrganismiBrassica oleracea (Wild cabbage)
Taxonomic identifieri3712 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 586585Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056921Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP49091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini194 – 517324Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated
Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49091-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILAVLGC SDDSQAKRVR VLELSRRLRH RGPDWSGIYQ NGFNYLAHQR
60 70 80 90 100
LAIIDPDSGD QPLFNEDKSI VVTVNGEIYN HEELRKGLKN HKFHTGSDCD
110 120 130 140 150
VIAHLYEEHG ENFVDMLDGI FSFVLLDTRD NSFMVARDAV GVTSLYIGWG
160 170 180 190 200
LDGSLWVSSE MKGLHEDCEH FEAFPPGHLY SSKSGGGFKQ WYNPPWFNES
210 220 230 240 250
VPSTPYEPLA IRSAFEDAVI KRLMTDVPFG VLLSGGLDSS LVASITARHL
260 270 280 290 300
AGTKAAKRWG PQLHSFCVGL EGSPDLKAGK EVAEYLGTVH HEFHFTVQDG
310 320 330 340 350
IDAIEDVIYH VETYDVTTIR ASTPMFLMSR KIKSLGVKMV LSGEGSDEIF
360 370 380 390 400
GGYLYFHKAP NKQEFHQETC RKIKALHKYD CLRANKATSA FGLEARVPFL
410 420 430 440 450
DKEFINTAMS LDPESKMIKP EEGRIEKWVL RRAFDDEERP YLPKHILYRQ
460 470 480 490 500
KEQFSDGVGY SWIDGLKAHA AENVNDKMMS KAAFIFPHNT PLTKEAYYYR
510 520 530 540 550
MIFERFFPQN SARLTVPGGA TVACSTAKAV EWDASWSNNM DPSGRAAIGV
560 570 580
HLSAYDGSKV ALPLPAPHKA IDDIPMMMGQ EVVIQT
Length:586
Mass (Da):65,673
Last modified:January 23, 2007 - v2
Checksum:iDC58CDE0DF3EF4BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84448 mRNA. Translation: CAA59138.1.
PIRiS52387.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84448 mRNA. Translation: CAA59138.1 .
PIRi S52387.

3D structure databases

ProteinModelPortali P49091.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An asparagine synthetase cDNA clone from Broccoli (Brassica oleracea L.)."
    Downs C.G., Pogson B.J., Davies K.M., Almira E.C.
    Plant Gene Register PGR95-016
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Shogun.

Entry informationi

Entry nameiASNS_BRAOL
AccessioniPrimary (citable) accession number: P49091
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3