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P49091 (ASNS_BRAOL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine synthetase [glutamine-hydrolyzing]

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase
OrganismBrassica oleracea (Wild cabbage)
Taxonomic identifier3712 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length586 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Belongs to the asparagine synthetase family.

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 586585Asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056921

Regions

Domain2 – 185184Glutamine amidotransferase type-2
Domain194 – 517324Asparagine synthetase
Nucleotide binding342 – 3432ATP By similarity
Region50 – 545Glutamine binding By similarity
Region75 – 773Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site981Glutamine By similarity
Binding site2321ATP; via carbonyl oxygen By similarity
Binding site2681ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3441Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
P49091 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: DC58CDE0DF3EF4BA

FASTA58665,673
        10         20         30         40         50         60 
MCGILAVLGC SDDSQAKRVR VLELSRRLRH RGPDWSGIYQ NGFNYLAHQR LAIIDPDSGD 

        70         80         90        100        110        120 
QPLFNEDKSI VVTVNGEIYN HEELRKGLKN HKFHTGSDCD VIAHLYEEHG ENFVDMLDGI 

       130        140        150        160        170        180 
FSFVLLDTRD NSFMVARDAV GVTSLYIGWG LDGSLWVSSE MKGLHEDCEH FEAFPPGHLY 

       190        200        210        220        230        240 
SSKSGGGFKQ WYNPPWFNES VPSTPYEPLA IRSAFEDAVI KRLMTDVPFG VLLSGGLDSS 

       250        260        270        280        290        300 
LVASITARHL AGTKAAKRWG PQLHSFCVGL EGSPDLKAGK EVAEYLGTVH HEFHFTVQDG 

       310        320        330        340        350        360 
IDAIEDVIYH VETYDVTTIR ASTPMFLMSR KIKSLGVKMV LSGEGSDEIF GGYLYFHKAP 

       370        380        390        400        410        420 
NKQEFHQETC RKIKALHKYD CLRANKATSA FGLEARVPFL DKEFINTAMS LDPESKMIKP 

       430        440        450        460        470        480 
EEGRIEKWVL RRAFDDEERP YLPKHILYRQ KEQFSDGVGY SWIDGLKAHA AENVNDKMMS 

       490        500        510        520        530        540 
KAAFIFPHNT PLTKEAYYYR MIFERFFPQN SARLTVPGGA TVACSTAKAV EWDASWSNNM 

       550        560        570        580 
DPSGRAAIGV HLSAYDGSKV ALPLPAPHKA IDDIPMMMGQ EVVIQT 

« Hide

References

[1]"An asparagine synthetase cDNA clone from Broccoli (Brassica oleracea L.)."
Downs C.G., Pogson B.J., Davies K.M., Almira E.C.
Plant Gene Register PGR95-016
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Shogun.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X84448 mRNA. Translation: CAA59138.1.
PIRS52387.

3D structure databases

ProteinModelPortalP49091.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.976.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNS_BRAOL
AccessionPrimary (citable) accession number: P49091
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways