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Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

ASN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 58200 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] 1 (ASN1), Asparagine synthetase [glutamine-hydrolyzing] 2 (ASN2)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei97GlutamineBy similarity1
Binding sitei233ATP; via carbonyl oxygenBy similarity1
Binding sitei291ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei367Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi365 – 366ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • asparagine biosynthetic process Source: SGD
  • glutamine metabolic process Source: UniProtKB-KW
  • L-asparagine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YGR124W-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 2
Gene namesi
Name:ASN2
Ordered Locus Names:YGR124W
ORF Names:G6358
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR124W.
SGDiS000003356. ASN2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569182 – 572Asparagine synthetase [glutamine-hydrolyzing] 2Add BLAST571

Proteomic databases

MaxQBiP49090.
PRIDEiP49090.

2D gel databases

UCD-2DPAGEiP49090.

PTM databases

iPTMnetiP49090.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33372. 31 interactors.
DIPiDIP-5189N.
IntActiP49090. 18 interactors.
MINTiMINT-501384.
STRINGi4932.YGR124W.

Structurei

3D structure databases

ProteinModelPortaliP49090.
SMRiP49090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 186Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST185
Domaini194 – 545Asparagine synthetaseAdd BLAST352

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Glutamine bindingBy similarity5
Regioni74 – 76Glutamine bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00390000001994.
HOGENOMiHOG000027493.
InParanoidiP49090.
KOiK01953.
OMAiYFHAAPD.
OrthoDBiEOG092C11PG.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFAAFKH EDIHNFKPKA LQLSKKIRHR GPDWSGNAVM NSTIFVHERL
60 70 80 90 100
AIVGLDSGAQ PITSADGEYM LGVNGEIYNH IQLREMCSDY KFQTFSDCEP
110 120 130 140 150
IIPLYLEHDI DAPKYLDGMF AFCLYDSKKD RIVAARDPIG VVTLYMGRSS
160 170 180 190 200
QSPETVYFAS ELKCLTDVCD SIISFPPGHV YDSETDKITR YFTPDWLDEK
210 220 230 240 250
RIPSTPVDYH AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIAAIAARE
260 270 280 290 300
TEKANADANE DNNVDEKQLA GIDDQGHLHT SGWSRLHSFA IGLPNAPDLQ
310 320 330 340 350
AARKVAKFIG SIHHEHTFTL QEGLDALDDV IYHLETYDVT TIRASTPMFL
360 370 380 390 400
LSRKIKAQGV KMVLSGEGSD EIFGGYLYFA QAPSAAEFHT ESVQRVKNLH
410 420 430 440 450
LADCLRANKS TMAWGLEARV PFLDKDFLQL CMNIDPNEKM IKPKEGRIEK
460 470 480 490 500
YILRKAFDTT DEPDVKPYLP EEILWRQKEQ FSDGVGYSWI DGLRDTAERA
510 520 530 540 550
ISDAMFANPK ADWGDDIPTT KEAYWYRLKF DAWFPQKTAA DTVMRWIPKA
560 570
DWGCAEDPSG RYAKIHEKHV SA
Length:572
Mass (Da):64,593
Last modified:January 23, 2007 - v2
Checksum:i2809F3F0FEDE4183
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti560G → S in AAT92877 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83099 Genomic DNA. Translation: CAA58159.1.
Z72909 Genomic DNA. Translation: CAA97135.1.
AY692858 Genomic DNA. Translation: AAT92877.1.
BK006941 Genomic DNA. Translation: DAA08217.1.
PIRiS55982.
RefSeqiNP_011640.1. NM_001181253.1.

Genome annotation databases

EnsemblFungiiYGR124W; YGR124W; YGR124W.
GeneIDi853025.
KEGGisce:YGR124W.

Similar proteinsi

Entry informationi

Entry nameiASNS2_YEAST
AccessioniPrimary (citable) accession number: P49090
Secondary accession number(s): D6VUQ6, E9P8Y0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names