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P49089

- ASNS1_YEAST

UniProt

P49089 - ASNS1_YEAST

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Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

ASN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei97 – 971GlutamineBy similarity
Binding sitei233 – 2331ATP; via carbonyl oxygenBy similarity
Binding sitei292 – 2921ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei368 – 3681Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi366 – 3672ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: SGD
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. asparagine biosynthetic process Source: SGD
  2. glutamine metabolic process Source: UniProtKB-KW
  3. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YPR145W-MONOMER.
ReactomeiREACT_233956. ATF4 activates genes.
REACT_245457. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Gene namesi
Name:ASN1
Ordered Locus Names:YPR145W
ORF Names:P9659.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

SGDiS000006349. ASN1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 572571Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000056917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei265 – 2651Phosphoserine1 Publication
Modified residuei509 – 5091Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49089.
PaxDbiP49089.
PeptideAtlasiP49089.

Expressioni

Gene expression databases

GenevestigatoriP49089.

Interactioni

Protein-protein interaction databases

BioGridi36314. 28 interactions.
DIPiDIP-3810N.
IntActiP49089. 3 interactions.
MINTiMINT-537542.
STRINGi4932.YPR145W.

Structurei

3D structure databases

ProteinModelPortaliP49089.
SMRiP49089. Positions 4-544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 186185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini194 – 546353Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 535Glutamine bindingBy similarity
Regioni74 – 763Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
GeneTreeiENSGT00390000001994.
HOGENOMiHOG000027493.
InParanoidiP49089.
KOiK01953.
OMAiRAAGHTF.
OrthoDBiEOG73JM46.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49089-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL
60 70 80 90 100
AIVGVESGAQ PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP
110 120 130 140 150
IIPMYLKHDI DAPKYLDGMF AWTLYDAKQD RIVAARDPIG ITTLYMGRSS
160 170 180 190 200
ASPKTVYFAS ELKCLTDDCD TITAFPPGHV YDSKTDKITR YFTPDWLDEK
210 220 230 240 250
RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIASIAARE
260 270 280 290 300
TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL
310 320 330 340 350
QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF
360 370 380 390 400
LLSRKIKAQG VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL
410 420 430 440 450
HLADCLRANK STMAWGLEAR VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE
460 470 480 490 500
KYILRKAFDT TGEPDAKPYL PEEILWRQKE QFSDGVGYSW IDGLKDTAEA
510 520 530 540 550
VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV ADTVMRWIPK
560 570
ADWGCAEDPS GRYAQIHEKH IE
Length:572
Mass (Da):64,470
Last modified:January 23, 2007 - v2
Checksum:i39F2873F0E689FC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48675 Genomic DNA. Translation: CAA88594.1.
U40829 Genomic DNA. Translation: AAB68284.1.
BK006949 Genomic DNA. Translation: DAA11558.1.
PIRiS52694.
RefSeqiNP_015471.1. NM_001184242.1.

Genome annotation databases

EnsemblFungiiYPR145W; YPR145W; YPR145W.
GeneIDi856268.
KEGGisce:YPR145W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48675 Genomic DNA. Translation: CAA88594.1 .
U40829 Genomic DNA. Translation: AAB68284.1 .
BK006949 Genomic DNA. Translation: DAA11558.1 .
PIRi S52694.
RefSeqi NP_015471.1. NM_001184242.1.

3D structure databases

ProteinModelPortali P49089.
SMRi P49089. Positions 4-544.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36314. 28 interactions.
DIPi DIP-3810N.
IntActi P49089. 3 interactions.
MINTi MINT-537542.
STRINGi 4932.YPR145W.

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

MaxQBi P49089.
PaxDbi P49089.
PeptideAtlasi P49089.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR145W ; YPR145W ; YPR145W .
GeneIDi 856268.
KEGGi sce:YPR145W.

Organism-specific databases

SGDi S000006349. ASN1.

Phylogenomic databases

eggNOGi COG0367.
GeneTreei ENSGT00390000001994.
HOGENOMi HOG000027493.
InParanoidi P49089.
KOi K01953.
OMAi RAAGHTF.
OrthoDBi EOG73JM46.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .
BioCyci YEAST:YPR145W-MONOMER.
Reactomei REACT_233956. ATF4 activates genes.
REACT_245457. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi 981571.
PROi P49089.

Gene expression databases

Genevestigatori P49089.

Family and domain databases

Gene3Di 3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the ASN1 and ASN2 genes encoding asparagine synthetases in Saccharomyces cerevisiae: differential regulation by the CCAAT-box-binding factor."
    Dang V.D., Valens M., Bolotin-Fukuhara M., Daignan-Fornier B.
    Mol. Microbiol. 22:681-692(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-265 AND SER-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiASNS1_YEAST
AccessioniPrimary (citable) accession number: P49089
Secondary accession number(s): D6W4E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3