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P49089

- ASNS1_YEAST

UniProt

P49089 - ASNS1_YEAST

Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

ASN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei97 – 971GlutamineBy similarity
    Binding sitei233 – 2331ATP; via carbonyl oxygenBy similarity
    Binding sitei292 – 2921ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei368 – 3681Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi366 – 3672ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: SGD
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. asparagine biosynthetic process Source: SGD
    2. glutamine metabolic process Source: UniProtKB-KW
    3. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YPR145W-MONOMER.
    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase 1
    Gene namesi
    Name:ASN1
    Ordered Locus Names:YPR145W
    ORF Names:P9659.3
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000006349. ASN1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 572571Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000056917Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei265 – 2651Phosphoserine1 Publication
    Modified residuei509 – 5091Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP49089.
    PaxDbiP49089.
    PeptideAtlasiP49089.

    Expressioni

    Gene expression databases

    GenevestigatoriP49089.

    Interactioni

    Protein-protein interaction databases

    BioGridi36314. 27 interactions.
    DIPiDIP-3810N.
    IntActiP49089. 3 interactions.
    MINTiMINT-537542.
    STRINGi4932.YPR145W.

    Structurei

    3D structure databases

    ProteinModelPortaliP49089.
    SMRiP49089. Positions 4-544.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 186185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini194 – 546353Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 535Glutamine bindingBy similarity
    Regioni74 – 763Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    GeneTreeiENSGT00390000001994.
    HOGENOMiHOG000027493.
    KOiK01953.
    OMAiRAAGHTF.
    OrthoDBiEOG73JM46.

    Family and domain databases

    Gene3Di3.40.50.620. 2 hits.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49089-1 [UniParc]FASTAAdd to Basket

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    MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL    50
    AIVGVESGAQ PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP 100
    IIPMYLKHDI DAPKYLDGMF AWTLYDAKQD RIVAARDPIG ITTLYMGRSS 150
    ASPKTVYFAS ELKCLTDDCD TITAFPPGHV YDSKTDKITR YFTPDWLDEK 200
    RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIASIAARE 250
    TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL 300
    QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF 350
    LLSRKIKAQG VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL 400
    HLADCLRANK STMAWGLEAR VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE 450
    KYILRKAFDT TGEPDAKPYL PEEILWRQKE QFSDGVGYSW IDGLKDTAEA 500
    VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV ADTVMRWIPK 550
    ADWGCAEDPS GRYAQIHEKH IE 572
    Length:572
    Mass (Da):64,470
    Last modified:January 23, 2007 - v2
    Checksum:i39F2873F0E689FC9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48675 Genomic DNA. Translation: CAA88594.1.
    U40829 Genomic DNA. Translation: AAB68284.1.
    BK006949 Genomic DNA. Translation: DAA11558.1.
    PIRiS52694.
    RefSeqiNP_015471.1. NM_001184242.1.

    Genome annotation databases

    EnsemblFungiiYPR145W; YPR145W; YPR145W.
    GeneIDi856268.
    KEGGisce:YPR145W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48675 Genomic DNA. Translation: CAA88594.1 .
    U40829 Genomic DNA. Translation: AAB68284.1 .
    BK006949 Genomic DNA. Translation: DAA11558.1 .
    PIRi S52694.
    RefSeqi NP_015471.1. NM_001184242.1.

    3D structure databases

    ProteinModelPortali P49089.
    SMRi P49089. Positions 4-544.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36314. 27 interactions.
    DIPi DIP-3810N.
    IntActi P49089. 3 interactions.
    MINTi MINT-537542.
    STRINGi 4932.YPR145W.

    Protein family/group databases

    MEROPSi C44.976.

    Proteomic databases

    MaxQBi P49089.
    PaxDbi P49089.
    PeptideAtlasi P49089.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR145W ; YPR145W ; YPR145W .
    GeneIDi 856268.
    KEGGi sce:YPR145W.

    Organism-specific databases

    SGDi S000006349. ASN1.

    Phylogenomic databases

    eggNOGi COG0367.
    GeneTreei ENSGT00390000001994.
    HOGENOMi HOG000027493.
    KOi K01953.
    OMAi RAAGHTF.
    OrthoDBi EOG73JM46.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .
    BioCyci YEAST:YPR145W-MONOMER.

    Miscellaneous databases

    NextBioi 981571.
    PROi P49089.

    Gene expression databases

    Genevestigatori P49089.

    Family and domain databases

    Gene3Di 3.40.50.620. 2 hits.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the ASN1 and ASN2 genes encoding asparagine synthetases in Saccharomyces cerevisiae: differential regulation by the CCAAT-box-binding factor."
      Dang V.D., Valens M., Bolotin-Fukuhara M., Daignan-Fornier B.
      Mol. Microbiol. 22:681-692(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-265 AND SER-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiASNS1_YEAST
    AccessioniPrimary (citable) accession number: P49089
    Secondary accession number(s): D6W4E2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3