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Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

ASN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] 1 (ASN1), Asparagine synthetase [glutamine-hydrolyzing] 2 (ASN2)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei97GlutamineBy similarity1
Binding sitei233ATP; via carbonyl oxygenBy similarity1
Binding sitei292ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei368Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi366 – 367ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • asparagine biosynthetic process Source: SGD
  • glutamine metabolic process Source: UniProtKB-KW
  • L-asparagine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YPR145W-MONOMER.
UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Gene namesi
Name:ASN1
Ordered Locus Names:YPR145W
ORF Names:P9659.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR145W.
SGDiS000006349. ASN1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569172 – 572Asparagine synthetase [glutamine-hydrolyzing] 1Add BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265PhosphoserineCombined sources1
Modified residuei509PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49089.
PRIDEiP49089.

PTM databases

iPTMnetiP49089.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36314. 72 interactors.
DIPiDIP-3810N.
IntActiP49089. 3 interactors.
MINTiMINT-537542.
STRINGi4932.YPR145W.

Structurei

3D structure databases

ProteinModelPortaliP49089.
SMRiP49089.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 186Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST185
Domaini194 – 546Asparagine synthetaseAdd BLAST353

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Glutamine bindingBy similarity5
Regioni74 – 76Glutamine bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00880000139190.
HOGENOMiHOG000027493.
InParanoidiP49089.
KOiK01953.
OMAiLFGYKYT.
OrthoDBiEOG092C11PG.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL
60 70 80 90 100
AIVGVESGAQ PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP
110 120 130 140 150
IIPMYLKHDI DAPKYLDGMF AWTLYDAKQD RIVAARDPIG ITTLYMGRSS
160 170 180 190 200
ASPKTVYFAS ELKCLTDDCD TITAFPPGHV YDSKTDKITR YFTPDWLDEK
210 220 230 240 250
RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIASIAARE
260 270 280 290 300
TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL
310 320 330 340 350
QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF
360 370 380 390 400
LLSRKIKAQG VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL
410 420 430 440 450
HLADCLRANK STMAWGLEAR VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE
460 470 480 490 500
KYILRKAFDT TGEPDAKPYL PEEILWRQKE QFSDGVGYSW IDGLKDTAEA
510 520 530 540 550
VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV ADTVMRWIPK
560 570
ADWGCAEDPS GRYAQIHEKH IE
Length:572
Mass (Da):64,470
Last modified:January 23, 2007 - v2
Checksum:i39F2873F0E689FC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48675 Genomic DNA. Translation: CAA88594.1.
U40829 Genomic DNA. Translation: AAB68284.1.
BK006949 Genomic DNA. Translation: DAA11558.1.
PIRiS52694.
RefSeqiNP_015471.1. NM_001184242.1.

Genome annotation databases

EnsemblFungiiYPR145W; YPR145W; YPR145W.
GeneIDi856268.
KEGGisce:YPR145W.

Similar proteinsi

Entry informationi

Entry nameiASNS1_YEAST
AccessioniPrimary (citable) accession number: P49089
Secondary accession number(s): D6W4E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names