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Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

ASN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] 1 (ASN1), Asparagine synthetase [glutamine-hydrolyzing] 2 (ASN2)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei97 – 971GlutamineBy similarity
Binding sitei233 – 2331ATP; via carbonyl oxygenBy similarity
Binding sitei292 – 2921ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei368 – 3681Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi366 – 3672ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • asparagine biosynthetic process Source: SGD
  • glutamine metabolic process Source: GO_Central
  • L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YPR145W-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Gene namesi
Name:ASN1
Ordered Locus Names:YPR145W
ORF Names:P9659.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR145W.
SGDiS000006349. ASN1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 572571Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000056917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei265 – 2651PhosphoserineCombined sources
Modified residuei509 – 5091PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP49089.

PTM databases

iPTMnetiP49089.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36314. 27 interactions.
DIPiDIP-3810N.
IntActiP49089. 3 interactions.
MINTiMINT-537542.

Structurei

3D structure databases

ProteinModelPortaliP49089.
SMRiP49089. Positions 4-544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 186185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini194 – 546353Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 535Glutamine bindingBy similarity
Regioni74 – 763Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00840000131667.
HOGENOMiHOG000027493.
InParanoidiP49089.
KOiK01953.
OMAiQHGDCYL.
OrthoDBiEOG092C11PG.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 2 hits.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL
60 70 80 90 100
AIVGVESGAQ PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP
110 120 130 140 150
IIPMYLKHDI DAPKYLDGMF AWTLYDAKQD RIVAARDPIG ITTLYMGRSS
160 170 180 190 200
ASPKTVYFAS ELKCLTDDCD TITAFPPGHV YDSKTDKITR YFTPDWLDEK
210 220 230 240 250
RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIASIAARE
260 270 280 290 300
TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL
310 320 330 340 350
QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF
360 370 380 390 400
LLSRKIKAQG VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL
410 420 430 440 450
HLADCLRANK STMAWGLEAR VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE
460 470 480 490 500
KYILRKAFDT TGEPDAKPYL PEEILWRQKE QFSDGVGYSW IDGLKDTAEA
510 520 530 540 550
VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV ADTVMRWIPK
560 570
ADWGCAEDPS GRYAQIHEKH IE
Length:572
Mass (Da):64,470
Last modified:January 23, 2007 - v2
Checksum:i39F2873F0E689FC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48675 Genomic DNA. Translation: CAA88594.1.
U40829 Genomic DNA. Translation: AAB68284.1.
BK006949 Genomic DNA. Translation: DAA11558.1.
PIRiS52694.
RefSeqiNP_015471.1. NM_001184242.1.

Genome annotation databases

EnsemblFungiiYPR145W; YPR145W; YPR145W.
GeneIDi856268.
KEGGisce:YPR145W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48675 Genomic DNA. Translation: CAA88594.1.
U40829 Genomic DNA. Translation: AAB68284.1.
BK006949 Genomic DNA. Translation: DAA11558.1.
PIRiS52694.
RefSeqiNP_015471.1. NM_001184242.1.

3D structure databases

ProteinModelPortaliP49089.
SMRiP49089. Positions 4-544.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36314. 27 interactions.
DIPiDIP-3810N.
IntActiP49089. 3 interactions.
MINTiMINT-537542.

Protein family/group databases

MEROPSiC44.976.

PTM databases

iPTMnetiP49089.

Proteomic databases

MaxQBiP49089.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR145W; YPR145W; YPR145W.
GeneIDi856268.
KEGGisce:YPR145W.

Organism-specific databases

EuPathDBiFungiDB:YPR145W.
SGDiS000006349. ASN1.

Phylogenomic databases

GeneTreeiENSGT00840000131667.
HOGENOMiHOG000027493.
InParanoidiP49089.
KOiK01953.
OMAiQHGDCYL.
OrthoDBiEOG092C11PG.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.
BioCyciYEAST:YPR145W-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiP49089.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 2 hits.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS1_YEAST
AccessioniPrimary (citable) accession number: P49089
Secondary accession number(s): D6W4E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.