Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic precursor

Preferred order of sections

Names and origin

Protein names

Recommended name:
Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic
Short name=AK-HD 2
Short name=AK-HSDH 2

Including the following 2 domains:

Aspartokinase
EC=2.7.2.4
Homoserine dehydrogenase
EC=1.1.1.3

Gene names

Name:

AKHSDH2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)⁺ = L-aspartate 4-semialdehyde + NAD(P)H. ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Subunit structure	Homo- or heterodimer Potential.
Subcellular location	Plastid › chloroplast.
Sequence similarities	In the N-terminal section; belongs to the aspartokinase family. In the C-terminal section; belongs to the homoserine dehydrogenase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Repeat Transit peptide
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NADP binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 89

89

Chloroplast Potential

Chain

90 – 917

828

Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic

PRO_0000002393

Regions

Domain

409 – 483

75

ACT 1

Domain

493 – 560

68

ACT 2

Nucleotide binding

565 – 570

6

NADP Potential

Region

90 – 338

249

Aspartokinase

Region

339 – 563

225

Interface

Region

564 – 917

354

Homoserine dehydrogenase

Sequences

Sequence

Length

Mass (Da)

Tools

P49080 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 69229EA13CAEA7E9
Show »

FASTA

917

99,583

        10         20         30         40         50         60 
MQGLAVSCQL PPAAAAARWR PRASSSNREA VLQCWKYELS QDHYLGGPLR IGQSQGSLHR 

        70         80         90        100        110        120 
HRSTNFLRPA AAAISVEQDE VNTYLPKGDM WSVHKFGGTC MGTPKRIQCV ANIVLGDSSE 

       130        140        150        160        170        180 
RKLIIVSAMS KVTDMMYNLV QKAQSRDDSY AIALAEVFEK HMTAAKDLLD GEDLARFLSQ 

       190        200        210        220        230        240 
LHSDVSNLRA MLRAIYIAGH ATESFSDFVV GHGELWSAQM LSYAIKKSGA PCSWMDTREV 

       250        260        270        280        290        300 
LVVTPSGCNQ VDPDYLECEK RLQKWFSRQP AEIIVATGFI ASTAGNIPTT LKRDGSDFSA 

       310        320        330        340        350        360 
AIVGSLVRAR QVTIWTDVDG VFSADPRKVS EAVILSTLSY QEAWEMSYFG ANVLHPRTII 

       370        380        390        400        410        420 
PVMKDNIPIV IRNMFNLSAP GTMICKQPAN ENGDLDACVK SFATVDNLAL VNVEGTGMAG 

       430        440        450        460        470        480 
VPGTASAIFS AVKDVGANVI MISQASSEHS VCFAVPEKEV AVVSAELHDR FREALAAGRL 

       490        500        510        520        530        540 
SKVEVINGCS ILAAVGLRMA STPGVSAILF DALAKANINV RAIAQGCSEY NITVVLKQQD 

       550        560        570        580        590        600 
CVRALRAAHS RFFLSKTTLA VGIIGPGLIG GALLNQLKNQ TAVLKENMNI DLRVIGITGS 

       610        620        630        640        650        660 
STMLLSDTGI DLTQWKQLLQ KEAEPADIGS FVHHLSDNHV FPNKVLVDCT ADTSVASHYY 

       670        680        690        700        710        720 
DWLKKGIHVI TPNKKANSGP LDQYLKLRTM QRASYTHYFY EATVGAGLPI ISTLRGLLET 

       730        740        750        760        770        780 
GDKILRIEGI FSGTLSYIFN NFEGTRAFSD VVAEAREAGY TEPDPRDDLS GTDVARKVVV 

       790        800        810        820        830        840 
LARESGLRLE LSDIPVKSLV PETLASCSSA DEFMQKLPSF DEDWARQRSD AEAAGEVLRY 

       850        860        870        880        890        900 
VGALDAVNRS GQVELRRYRR DHPFAQLSGS DNIIAFTTSR YKEQPLIVRG PGAGAEVTAG 

       910 
GVFCDILRLA SYLGAPS

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References

[1]	"Molecular genetics of the maize (Zea mays L.) aspartate kinase-homoserine dehydrogenase gene family." Muehlbauer G.J., Somers D.A., Matthews B.F., Gengenbach B.G. Plant Physiol. 106:1303-1312(1994) [PubMed: 7846152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seedling leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33913 mRNA. Translation: AAA74361.1.
PIR	T02954.
RefSeq	NP_001105691.1. NM_001112221.1.
UniGene	Zm.97211.
3D structure databases
ProteinModelPortal	P49080.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GRMZM2G104546_T02; GRMZM2G104546_P02; GRMZM2G104546.
GeneID	542708.
KEGG	zma:542708.
Organism-specific databases
Gramene	P49080.
MaizeGDB	66609.
Phylogenomic databases
GeneTree	EPGT00070000029599.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005106. Asp/hSer_DH_NAD-bd. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. IPR011147. Bifunc_aspartokin/hSer_DH. IPR001342. HDH_cat. IPR019811. HDH_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 2 hits. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K12524.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000727. ThrA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00657. Asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKH2_MAIZE

Accession

Primary (citable) accession number: P49080

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents