Reviewed, UniProtKB/Swiss-Prot P49080 (AKH2_MAIZE)

Names and origin

Protein names

Recommended name:
    Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic
      Short name=AK-HSDH 2
      Short name=AK-HD 2
Including the following 2 domains:
    1- Recommended name:
            Aspartokinase
              EC=2.7.2.4
    2- Recommended name:
            Homoserine dehydrogenase
              EC=1.1.1.3

Gene names

Name:

AKHSDH2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H. ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Subunit structure	Homo- or heterodimer Potential.
Subcellular location	Plastid › chloroplast.
Sequence similarities	In the N-terminal section; belongs to the aspartokinase family. In the C-terminal section; belongs to the homoserine dehydrogenase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Repeat Transit peptide
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 89	89	Chloroplast Potential
	Chain	90 – 917	828	Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic		PRO_0000002393
Regions
	Domain	409 – 483	75	ACT 1
	Domain	493 – 560	68	ACT 2
	Nucleotide binding	565 – 570	6	NADP Potential
	Region	90 – 338	249	Aspartokinase
	Region	339 – 563	225	Interface
	Region	564 – 917	354	Homoserine dehydrogenase

Sequences

	Sequence		Length	Mass (Da)
	P49080-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 69229EA13CAEA7E9	FASTA	917	99,583
	10 20 30 40 50 60 MQGLAVSCQL PPAAAAARWR PRASSSNREA VLQCWKYELS QDHYLGGPLR IGQSQGSLHR 70 80 90 100 110 120 HRSTNFLRPA AAAISVEQDE VNTYLPKGDM WSVHKFGGTC MGTPKRIQCV ANIVLGDSSE 130 140 150 160 170 180 RKLIIVSAMS KVTDMMYNLV QKAQSRDDSY AIALAEVFEK HMTAAKDLLD GEDLARFLSQ 190 200 210 220 230 240 LHSDVSNLRA MLRAIYIAGH ATESFSDFVV GHGELWSAQM LSYAIKKSGA PCSWMDTREV 250 260 270 280 290 300 LVVTPSGCNQ VDPDYLECEK RLQKWFSRQP AEIIVATGFI ASTAGNIPTT LKRDGSDFSA 310 320 330 340 350 360 AIVGSLVRAR QVTIWTDVDG VFSADPRKVS EAVILSTLSY QEAWEMSYFG ANVLHPRTII 370 380 390 400 410 420 PVMKDNIPIV IRNMFNLSAP GTMICKQPAN ENGDLDACVK SFATVDNLAL VNVEGTGMAG 430 440 450 460 470 480 VPGTASAIFS AVKDVGANVI MISQASSEHS VCFAVPEKEV AVVSAELHDR FREALAAGRL 490 500 510 520 530 540 SKVEVINGCS ILAAVGLRMA STPGVSAILF DALAKANINV RAIAQGCSEY NITVVLKQQD 550 560 570 580 590 600 CVRALRAAHS RFFLSKTTLA VGIIGPGLIG GALLNQLKNQ TAVLKENMNI DLRVIGITGS 610 620 630 640 650 660 STMLLSDTGI DLTQWKQLLQ KEAEPADIGS FVHHLSDNHV FPNKVLVDCT ADTSVASHYY 670 680 690 700 710 720 DWLKKGIHVI TPNKKANSGP LDQYLKLRTM QRASYTHYFY EATVGAGLPI ISTLRGLLET 730 740 750 760 770 780 GDKILRIEGI FSGTLSYIFN NFEGTRAFSD VVAEAREAGY TEPDPRDDLS GTDVARKVVV 790 800 810 820 830 840 LARESGLRLE LSDIPVKSLV PETLASCSSA DEFMQKLPSF DEDWARQRSD AEAAGEVLRY 850 860 870 880 890 900 VGALDAVNRS GQVELRRYRR DHPFAQLSGS DNIIAFTTSR YKEQPLIVRG PGAGAEVTAG 910 GVFCDILRLA SYLGAPS

References

[1]	"Molecular genetics of the maize (Zea mays L.) aspartate kinase-homoserine dehydrogenase gene family." Muehlbauer G.J., Somers D.A., Matthews B.F., Gengenbach B.G. Plant Physiol. 106:1303-1312(1994) [PubMed: 7846152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seedling leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L33913 mRNA. Translation: AAA74361.1.
PIR	T02954.
RefSeq	NP_001105691.1.
UniGene	Zm.97211
3D structure databases
HSSP	HSSP built from PDB template 1EBF based on UniProtKB P31116.
ModBase	Search...
Genome annotation databases
GeneID	542708.
Organism-specific databases
Gramene	P49080.
MaizeGDB	66609.
Enzyme and pathway databases
BRENDA	1.1.1.3. 289. 2.7.2.4. 289.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005106. Asp/hSer_DH_NAD-bd. IPR001341. Asp_kin_reg. IPR018042. Aspartate_kinase_CS. IPR011147. bifunc_aspartokin/hSer_DH. IPR001342. Homoserine_dehydrogenase_cat. IPR019811. Homoserine_dehydrogenase_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000727. ThrA. 1 hit.
TIGRFAMs	TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKH2_MAIZE

Accession

Primary (citable) accession number: P49080

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)