Reviewed, UniProtKB/Swiss-Prot P49079 (AKH1_MAIZE)

Names and origin

Protein names

Recommended name:
    Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic
      Short name=AK-HSDH 1
      Short name=AK-HD 1
Including the following 2 domains:
    1- Recommended name:
            Aspartokinase
              EC=2.7.2.4
    2- Recommended name:
            Homoserine dehydrogenase
              EC=1.1.1.3

Gene names

Name:

AKHSDH1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H. ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Subunit structure	Homo- or heterodimer Potential.
Subcellular location	Plastid › chloroplast.
Sequence similarities	In the N-terminal section; belongs to the aspartokinase family. In the C-terminal section; belongs to the homoserine dehydrogenase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Repeat Transit peptide
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 92	92	Chloroplast Potential
	Chain	93 – 920	828	Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic		PRO_0000002392
Regions
	Domain	412 – 480	69	ACT 1
	Domain	496 – 563	68	ACT 2
	Nucleotide binding	568 – 573	6	NADP Potential
	Region	93 – 341	249	Aspartokinase
	Region	342 – 566	225	Interface
	Region	567 – 920	354	Homoserine dehydrogenase

Sequences

	Sequence		Length	Mass (Da)
	P49079-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 08DCF444BE645529	FASTA	920	100,336
	10 20 30 40 50 60 MRSLTVASRH PGAAFSTRRR PLLHPAAAGR DSTFQRCWRW EKTQDSSFGS SLRTSRLPRT 70 80 90 100 110 120 VHGDILKNLL APTAGAVSVE QAEAIADLPK GDMWSVHKFG GTCMGTSERI HNVADIVLRD 130 140 150 160 170 180 PSERKLVVVS AMSKVTDMMY NLVNKAQSRD DSYIAVLDEV FDKHMTTAKD LLAGEDLARF 190 200 210 220 230 240 LSQLHADISN LKAMLRAIYI AGHATESFSD FVVGHGELWS AQMLSYAIQK SGTPCSWMDT 250 260 270 280 290 300 REVLVVNPSG ANQVDPDYLE SEKRLEKWFS RCPAETIIAT GFIASTPENI PTTLKRDGSD 310 320 330 340 350 360 FSAAIIGSLV KARQVTIWTD VDGVFSADPR KVSEAVILST LSYQEAWEMS YFGANVLHPR 370 380 390 400 410 420 TIIPVMKYNI PIVIRNIFNT SAPGTMICQQ PANENGDLEA CVKAFATIDK LALVNVEGTG 430 440 450 460 470 480 MAGVPGTANA IFGAVKDVGA NVIMISQASS EHSVCFAVPE KEVALVSAAL HARFREALAA 490 500 510 520 530 540 GRLSKVEVIH NCSILATVGL RMASTPGVSA TLFDALAKAN INVRAIAQGC SEYNITIVLK 550 560 570 580 590 600 QEDCVRALRA AHSRFFLSKT TLAVGIIGPG LIGRTLLNQL KDQAAVLKEN MNIDLRVMGI 610 620 630 640 650 660 AGSRTMLLSD IGVDLTQWKE KLQTEAEPAN LDKFVHHLSE NHFFPNRVLV DCTADTSVAS 670 680 690 700 710 720 HYYDWLKKGI HVITPNKKAN SGPLDRYLKL RTLQRASYTH YFYEATVGAG LPIISTLRGL 730 740 750 760 770 780 LETGDKILRI EGIFSGTLSY IFNNFEGART FSDVVAEAKK AGYTEPDPRD DLSGTDVARK 790 800 810 820 830 840 VIILARESGL GLELSDIPVR SLVPEALKSC TSADEYMQKL PSFDEDWARE RKNAEAAGEV 850 860 870 880 890 900 LRYVGVVDVV SKKGQVELRA YKRDHPFAQL SGSDNIIAFT TSRYKDQPLI VRGPGAGAEV 910 920 TAGGVFCDIL RLSSYLGAPS

References

[1]	"Molecular genetics of the maize (Zea mays L.) aspartate kinase-homoserine dehydrogenase gene family." Muehlbauer G.J., Somers D.A., Matthews B.F., Gengenbach B.G. Plant Physiol. 106:1303-1312(1994) [PubMed: 7846152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seedling leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L33912 mRNA. Translation: AAA74360.1.
PIR	T02953.
RefSeq	NP_001105325.1.
UniGene	Zm.94547
3D structure databases
HSSP	HSSP built from PDB template 1EBF based on UniProtKB P31116.
ModBase	Search...
Genome annotation databases
GeneID	542249.
Organism-specific databases
Gramene	P49079.
MaizeGDB	66609.
Enzyme and pathway databases
BRENDA	1.1.1.3. 289. 2.7.2.4. 289.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005106. Asp/hSer_DH_NAD-bd. IPR001341. Asp_kin_reg. IPR018042. Aspartate_kinase_CS. IPR001342. Homoserine_dehydrogenase_cat. IPR019811. Homoserine_dehydrogenase_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKH1_MAIZE

Accession

Primary (citable) accession number: P49079

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)