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Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

ASN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.1 Publication

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] 1 (ASN1)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei98GlutamineBy similarity1
Binding sitei231ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei343Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 342ATPBy similarity2

GO - Molecular functioni

  • asparagine synthase (glutamine-hydrolyzing) activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • protein homodimerization activity Source: GO_Central

GO - Biological processi

  • asparagine biosynthetic process Source: UniProtKB
  • cellular amino acid catabolic process Source: TAIR
  • cellular response to sucrose starvation Source: TAIR
  • glutamine metabolic process Source: GO_Central
  • L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  • response to absence of light Source: TAIR
  • response to fructose Source: TAIR
  • response to glucose Source: TAIR
  • response to sucrose Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Protein DARK INDUCIBLE 6
Gene namesi
Name:ASN1
Synonyms:DIN6
Ordered Locus Names:At3g47340
ORF Names:T21L8.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G47340.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569192 – 584Asparagine synthetase [glutamine-hydrolyzing] 1Add BLAST583

Proteomic databases

PaxDbiP49078.
PRIDEiP49078.

Expressioni

Inductioni

By dark. Down-regulated by light and sucrose.1 Publication

Gene expression databases

ExpressionAtlasiP49078. baseline and differential.
GenevisibleiP49078. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT3G47340.1.

Structurei

3D structure databases

ProteinModelPortaliP49078.
SMRiP49078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST184
Domaini193 – 516Asparagine synthetaseAdd BLAST324

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571. Eukaryota.
COG0367. LUCA.
HOGENOMiHOG000027493.
InParanoidiP49078.
KOiK01953.
OMAiDFINTAM.
OrthoDBiEOG093604QK.
PhylomeDBiP49078.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P49078-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGILAVLGC SDDSQAKRVR VLELSRRLRH RGPDWSGLYQ NGDNYLAHQR
60 70 80 90 100
LAVIDPASGD QPLFNEDKTI VVTVNGEIYN HEELRKRLKN HKFRTGSDCE
110 120 130 140 150
VIAHLYEEYG VDFVDMLDGI FSFVLLDTRD NSFMVARDAI GVTSLYIGWG
160 170 180 190 200
LDGSVWISSE MKGLNDDCEH FETFPPGHFY SSKLGGFKQW YNPPWFNESV
210 220 230 240 250
PSTPYEPLAI RRAFENAVIK RLMTDVPFGV LLSGGLDSSL VASITARHLA
260 270 280 290 300
GTKAAKQWGP QLHSFCVGLE GSPDLKAGKE VAEYLGTVHH EFHFSVQDGI
310 320 330 340 350
DAIEDVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGADEIFG
360 370 380 390 400
GYLYFHKAPN KKEFHQETCR KIKALHKYDC LRANKSTSAF GLEARVPFLD
410 420 430 440 450
KDFINTAMSL DPESKMIKPE EGRIEKWVLR RAFDDEERPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKDHAA QNVNDKMMSN AGHIFPHNTP NTKEAYYYRM
510 520 530 540 550
IFERFFPQNS ARLTVPGGAT VACSTAKAVE WDASWSNNMD PSGRAAIGVH
560 570 580
LSAYDGKNVA LTIPPLKAID NMPMMMGQGV VIQS
Length:584
Mass (Da):65,621
Last modified:January 23, 2007 - v2
Checksum:i071910E4C6D02433
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29083 mRNA. Translation: AAA74359.1.
AL096860 Genomic DNA. Translation: CAB51206.1.
CP002686 Genomic DNA. Translation: AEE78264.1.
AF419557 mRNA. Translation: AAL31889.1.
AY072214 mRNA. Translation: AAL60035.1.
AY096592 mRNA. Translation: AAM20242.1.
PIRiT12989.
RefSeqiNP_190318.1. NM_114602.4. [P49078-1]
UniGeneiAt.20782.

Genome annotation databases

EnsemblPlantsiAT3G47340.1; AT3G47340.1; AT3G47340. [P49078-1]
GeneIDi823888.
GrameneiAT3G47340.1; AT3G47340.1; AT3G47340.
KEGGiath:AT3G47340.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29083 mRNA. Translation: AAA74359.1.
AL096860 Genomic DNA. Translation: CAB51206.1.
CP002686 Genomic DNA. Translation: AEE78264.1.
AF419557 mRNA. Translation: AAL31889.1.
AY072214 mRNA. Translation: AAL60035.1.
AY096592 mRNA. Translation: AAM20242.1.
PIRiT12989.
RefSeqiNP_190318.1. NM_114602.4. [P49078-1]
UniGeneiAt.20782.

3D structure databases

ProteinModelPortaliP49078.
SMRiP49078.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G47340.1.

Proteomic databases

PaxDbiP49078.
PRIDEiP49078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G47340.1; AT3G47340.1; AT3G47340. [P49078-1]
GeneIDi823888.
GrameneiAT3G47340.1; AT3G47340.1; AT3G47340.
KEGGiath:AT3G47340.

Organism-specific databases

TAIRiAT3G47340.

Phylogenomic databases

eggNOGiKOG0571. Eukaryota.
COG0367. LUCA.
HOGENOMiHOG000027493.
InParanoidiP49078.
KOiK01953.
OMAiDFINTAM.
OrthoDBiEOG093604QK.
PhylomeDBiP49078.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.
ReactomeiR-ATH-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiP49078.

Gene expression databases

ExpressionAtlasiP49078. baseline and differential.
GenevisibleiP49078. AT.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS1_ARATH
AccessioniPrimary (citable) accession number: P49078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants over-expressing ASN1 have increased content of free amino acids (mainly Asn) in flowers, siliques and seeds.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.