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P49078

- ASNS1_ARATH

UniProt

P49078 - ASNS1_ARATH

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Protein

Asparagine synthetase [glutamine-hydrolyzing] 1

Gene

ASN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.1 Publication

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei98 – 981GlutamineBy similarity
Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: TAIR
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. asparagine biosynthetic process Source: UniProtKB
  2. cellular amino acid catabolic process Source: TAIR
  3. cellular response to sucrose starvation Source: TAIR
  4. glutamine metabolic process Source: UniProtKB-KW
  5. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  6. response to absence of light Source: TAIR
  7. response to fructose Source: TAIR
  8. response to glucose Source: TAIR
  9. response to sucrose Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1413-MONOMER.
ARA:GQT-2583-MONOMER.
ReactomeiREACT_232680. Amino acid synthesis and interconversion (transamination).
REACT_254539. ATF4 activates genes.
UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 1
Protein DARK INDUCIBLE 6
Gene namesi
Name:ASN1
Synonyms:DIN6
Ordered Locus Names:At3g47340
ORF Names:T21L8.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G47340.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 584583Asparagine synthetase [glutamine-hydrolyzing] 1PRO_0000056919Add
BLAST

Proteomic databases

PaxDbiP49078.
PRIDEiP49078.

Expressioni

Inductioni

By dark. Down-regulated by light and sucrose.1 Publication

Gene expression databases

ExpressionAtlasiP49078. baseline and differential.
GenevestigatoriP49078.

Structurei

3D structure databases

ProteinModelPortaliP49078.
SMRiP49078. Positions 4-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini193 – 516324Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027493.
InParanoidiP49078.
KOiK01953.
OMAiDFINTAM.
PhylomeDBiP49078.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P49078-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGILAVLGC SDDSQAKRVR VLELSRRLRH RGPDWSGLYQ NGDNYLAHQR
60 70 80 90 100
LAVIDPASGD QPLFNEDKTI VVTVNGEIYN HEELRKRLKN HKFRTGSDCE
110 120 130 140 150
VIAHLYEEYG VDFVDMLDGI FSFVLLDTRD NSFMVARDAI GVTSLYIGWG
160 170 180 190 200
LDGSVWISSE MKGLNDDCEH FETFPPGHFY SSKLGGFKQW YNPPWFNESV
210 220 230 240 250
PSTPYEPLAI RRAFENAVIK RLMTDVPFGV LLSGGLDSSL VASITARHLA
260 270 280 290 300
GTKAAKQWGP QLHSFCVGLE GSPDLKAGKE VAEYLGTVHH EFHFSVQDGI
310 320 330 340 350
DAIEDVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGADEIFG
360 370 380 390 400
GYLYFHKAPN KKEFHQETCR KIKALHKYDC LRANKSTSAF GLEARVPFLD
410 420 430 440 450
KDFINTAMSL DPESKMIKPE EGRIEKWVLR RAFDDEERPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKDHAA QNVNDKMMSN AGHIFPHNTP NTKEAYYYRM
510 520 530 540 550
IFERFFPQNS ARLTVPGGAT VACSTAKAVE WDASWSNNMD PSGRAAIGVH
560 570 580
LSAYDGKNVA LTIPPLKAID NMPMMMGQGV VIQS
Length:584
Mass (Da):65,621
Last modified:January 23, 2007 - v2
Checksum:i071910E4C6D02433
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29083 mRNA. Translation: AAA74359.1.
AL096860 Genomic DNA. Translation: CAB51206.1.
CP002686 Genomic DNA. Translation: AEE78264.1.
AF419557 mRNA. Translation: AAL31889.1.
AY072214 mRNA. Translation: AAL60035.1.
AY096592 mRNA. Translation: AAM20242.1.
PIRiT12989.
RefSeqiNP_190318.1. NM_114602.3. [P49078-1]
UniGeneiAt.20782.

Genome annotation databases

EnsemblPlantsiAT3G47340.1; AT3G47340.1; AT3G47340. [P49078-1]
GeneIDi823888.
KEGGiath:AT3G47340.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29083 mRNA. Translation: AAA74359.1 .
AL096860 Genomic DNA. Translation: CAB51206.1 .
CP002686 Genomic DNA. Translation: AEE78264.1 .
AF419557 mRNA. Translation: AAL31889.1 .
AY072214 mRNA. Translation: AAL60035.1 .
AY096592 mRNA. Translation: AAM20242.1 .
PIRi T12989.
RefSeqi NP_190318.1. NM_114602.3. [P49078-1 ]
UniGenei At.20782.

3D structure databases

ProteinModelPortali P49078.
SMRi P49078. Positions 4-517.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

PaxDbi P49078.
PRIDEi P49078.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G47340.1 ; AT3G47340.1 ; AT3G47340 . [P49078-1 ]
GeneIDi 823888.
KEGGi ath:AT3G47340.

Organism-specific databases

TAIRi AT3G47340.

Phylogenomic databases

eggNOGi COG0367.
HOGENOMi HOG000027493.
InParanoidi P49078.
KOi K01953.
OMAi DFINTAM.
PhylomeDBi P49078.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .
BioCyci ARA:GQT-1413-MONOMER.
ARA:GQT-2583-MONOMER.
Reactomei REACT_232680. Amino acid synthesis and interconversion (transamination).
REACT_254539. ATF4 activates genes.

Gene expression databases

ExpressionAtlasi P49078. baseline and differential.
Genevestigatori P49078.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Metabolic regulation of the gene encoding glutamine-dependent asparagine synthetase in Arabidopsis thaliana."
    Lam H.M., Peng S.S., Coruzzi G.M.
    Plant Physiol. 106:1347-1357(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Reciprocal regulation of distinct asparagine synthetase genes by light and metabolites in Arabidopsis thaliana."
    Lam H.M., Hsieh M.H., Coruzzi G.
    Plant J. 16:345-353(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  6. "Overexpression of the ASN1 gene enhances nitrogen status in seeds of Arabidopsis."
    Lam H.M., Wong P., Chan H.K., Yam K.M., Chen L., Chow C.M., Coruzzi G.M.
    Plant Physiol. 132:926-935(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiASNS1_ARATH
AccessioniPrimary (citable) accession number: P49078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants over-expressing ASN1 have increased content of free amino acids (mainly Asn) in flowers, siliques and seeds.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3