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Protein

Aspartate carbamoyltransferase, chloroplastic

Gene

PYRB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Enzyme regulationi

Allosterically regulated by UMP.By similarity

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
  2. Aspartate carbamoyltransferase, chloroplastic (PYRB)
  3. Dihydroorotase, mitochondrial (PYR4)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Transferase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G20330-MONOMER
MetaCyc:AT3G20330-MONOMER
UniPathwayiUPA00070; UER00116

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase, chloroplastic (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:PYRB
Ordered Locus Names:At3g20330
ORF Names:MQC12.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G20330
TAIRilocus:2092369 AT3G20330

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 68ChloroplastSequence analysisAdd BLAST68
ChainiPRO_000002034869 – 390Aspartate carbamoyltransferase, chloroplasticAdd BLAST322

Proteomic databases

PaxDbiP49077
PRIDEiP49077

PTM databases

SwissPalmiP49077

Expressioni

Gene expression databases

ExpressionAtlasiP49077 baseline and differential
GenevisibleiP49077 AT

Interactioni

Subunit structurei

Homotrimer.Curated

Protein-protein interaction databases

BioGridi6909, 2 interactors
IntActiP49077, 1 interactor
STRINGi3702.AT3G20330.1

Structurei

3D structure databases

ProteinModelPortaliP49077
SMRiP49077
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IUMU Eukaryota
COG0540 LUCA
HOGENOMiHOG000022685
InParanoidiP49077
KOiK00609
OMAiWCDVANM
OrthoDBiEOG09360G1O
PhylomeDBiP49077

Family and domain databases

Gene3Di3.40.50.1370, 3 hits
HAMAPiMF_00001 Asp_carb_tr, 1 hit
InterProiView protein in InterPro
IPR006132 Asp/Orn_carbamoyltranf_P-bd
IPR006130 Asp/Orn_carbamoylTrfase
IPR036901 Asp/Orn_carbamoylTrfase_sf
IPR002082 Asp_carbamoyltransf
IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
PANTHERiPTHR11405:SF16 PTHR11405:SF16, 1 hit
PfamiView protein in Pfam
PF00185 OTCace, 1 hit
PF02729 OTCace_N, 1 hit
PRINTSiPR00100 AOTCASE
PR00101 ATCASE
SUPFAMiSSF53671 SSF53671, 1 hit
TIGRFAMsiTIGR00670 asp_carb_tr, 1 hit
PROSITEiView protein in PROSITE
PS00097 CARBAMOYLTRANSFERASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIASSLTSA TLCGASVFPK ALACSSEFPI NLPSPFESSK ICLTSFPASR
60 70 80 90 100
DLKKNATLNL TRNVGPVRCH AMQAGTRELK KFELSDVIEG KQFDREMLSA
110 120 130 140 150
IFDVAREMEK IEKSSSQSEI LKGYLMATLF YEPSTRTRLS FESAMKRLGG
160 170 180 190 200
EVLTTENARE FSSAAKGETL EDTIRTVEGY SDIIVMRHFE SGAARKAAAT
210 220 230 240 250
ANIPVINAGD GPGEHPTQAL LDVYTIQSEI GKLDGISVAL VGDLANGRTV
260 270 280 290 300
RSLAYLLAKF KDVKIYFVSP EIVKMKDDIK DYLTSSGVEW EESSDLMEVA
310 320 330 340 350
SKCDVVYQTR IQRERFGERL DLYEAARGKY IVDKDLLGVM QKKAIIMHPL
360 370 380 390
PRLDEITADV DADPRAAYFR QAKNGLFIRM ALLKLLLVGW
Length:390
Mass (Da):43,167
Last modified:July 11, 2001 - v2
Checksum:iEE8C9216629E826E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225T → N in AAK91399 (PubMed:14593172).Curated1
Sequence conflicti225T → N in AAL90999 (PubMed:14593172).Curated1
Sequence conflicti330Y → F in CAA50687 (PubMed:10819329).Curated1
Sequence conflicti379R → S in AAK91399 (PubMed:14593172).Curated1
Sequence conflicti379R → S in AAL90999 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71843 mRNA Translation: CAA50687.1
AB024036 Genomic DNA Translation: BAB02813.1
CP002686 Genomic DNA Translation: AEE76365.1
CP002686 Genomic DNA Translation: ANM64914.1
AY050381 mRNA Translation: AAK91399.1
AY090338 mRNA Translation: AAL90999.1
AK175118 mRNA Translation: BAD42881.1
PIRiS46441
RefSeqiNP_001326915.1, NM_001338476.1
NP_188668.1, NM_112924.4
UniGeneiAt.20992

Genome annotation databases

EnsemblPlantsiAT3G20330.1; AT3G20330.1; AT3G20330
AT3G20330.2; AT3G20330.2; AT3G20330
GeneIDi821577
GrameneiAT3G20330.1; AT3G20330.1; AT3G20330
AT3G20330.2; AT3G20330.2; AT3G20330
KEGGiath:AT3G20330

Similar proteinsi

Entry informationi

Entry nameiPYRB_ARATH
AccessioniPrimary (citable) accession number: P49077
Secondary accession number(s): Q683J9, Q94A47, Q9LTR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 11, 2001
Last modified: April 25, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health