Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartate carbamoyltransferase, chloroplastic

Gene

PYRB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Enzyme regulationi

Allosterically regulated by UMP.By similarity

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
  2. Aspartate carbamoyltransferase, chloroplastic (PYRB)
  3. Dihydroorotase, mitochondrial (PYR4)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G20330-MONOMER.
MetaCyc:AT3G20330-MONOMER.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase, chloroplastic (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:PYRB
Ordered Locus Names:At3g20330
ORF Names:MQC12.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G20330.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 68ChloroplastSequence analysisAdd BLAST68
ChainiPRO_000002034869 – 390Aspartate carbamoyltransferase, chloroplasticAdd BLAST322

Proteomic databases

PaxDbiP49077.
PRIDEiP49077.

PTM databases

SwissPalmiP49077.

Expressioni

Gene expression databases

GenevisibleiP49077. AT.

Interactioni

Subunit structurei

Homotrimer.Curated

Protein-protein interaction databases

BioGridi6909. 2 interactors.
IntActiP49077. 1 interactor.
STRINGi3702.AT3G20330.1.

Structurei

3D structure databases

ProteinModelPortaliP49077.
SMRiP49077.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IUMU. Eukaryota.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiP49077.
KOiK00609.
OMAiKQSFYGV.
OrthoDBiEOG09360G1O.
PhylomeDBiP49077.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIASSLTSA TLCGASVFPK ALACSSEFPI NLPSPFESSK ICLTSFPASR
60 70 80 90 100
DLKKNATLNL TRNVGPVRCH AMQAGTRELK KFELSDVIEG KQFDREMLSA
110 120 130 140 150
IFDVAREMEK IEKSSSQSEI LKGYLMATLF YEPSTRTRLS FESAMKRLGG
160 170 180 190 200
EVLTTENARE FSSAAKGETL EDTIRTVEGY SDIIVMRHFE SGAARKAAAT
210 220 230 240 250
ANIPVINAGD GPGEHPTQAL LDVYTIQSEI GKLDGISVAL VGDLANGRTV
260 270 280 290 300
RSLAYLLAKF KDVKIYFVSP EIVKMKDDIK DYLTSSGVEW EESSDLMEVA
310 320 330 340 350
SKCDVVYQTR IQRERFGERL DLYEAARGKY IVDKDLLGVM QKKAIIMHPL
360 370 380 390
PRLDEITADV DADPRAAYFR QAKNGLFIRM ALLKLLLVGW
Length:390
Mass (Da):43,167
Last modified:July 11, 2001 - v2
Checksum:iEE8C9216629E826E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225T → N in AAK91399 (PubMed:14593172).Curated1
Sequence conflicti225T → N in AAL90999 (PubMed:14593172).Curated1
Sequence conflicti330Y → F in CAA50687 (PubMed:10819329).Curated1
Sequence conflicti379R → S in AAK91399 (PubMed:14593172).Curated1
Sequence conflicti379R → S in AAL90999 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71843 mRNA. Translation: CAA50687.1.
AB024036 Genomic DNA. Translation: BAB02813.1.
CP002686 Genomic DNA. Translation: AEE76365.1.
AY050381 mRNA. Translation: AAK91399.1.
AY090338 mRNA. Translation: AAL90999.1.
AK175118 mRNA. Translation: BAD42881.1.
PIRiS46441.
RefSeqiNP_188668.1. NM_112924.4.
UniGeneiAt.20992.

Genome annotation databases

EnsemblPlantsiAT3G20330.1; AT3G20330.1; AT3G20330.
GeneIDi821577.
GrameneiAT3G20330.1; AT3G20330.1; AT3G20330.
KEGGiath:AT3G20330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71843 mRNA. Translation: CAA50687.1.
AB024036 Genomic DNA. Translation: BAB02813.1.
CP002686 Genomic DNA. Translation: AEE76365.1.
AY050381 mRNA. Translation: AAK91399.1.
AY090338 mRNA. Translation: AAL90999.1.
AK175118 mRNA. Translation: BAD42881.1.
PIRiS46441.
RefSeqiNP_188668.1. NM_112924.4.
UniGeneiAt.20992.

3D structure databases

ProteinModelPortaliP49077.
SMRiP49077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6909. 2 interactors.
IntActiP49077. 1 interactor.
STRINGi3702.AT3G20330.1.

PTM databases

SwissPalmiP49077.

Proteomic databases

PaxDbiP49077.
PRIDEiP49077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G20330.1; AT3G20330.1; AT3G20330.
GeneIDi821577.
GrameneiAT3G20330.1; AT3G20330.1; AT3G20330.
KEGGiath:AT3G20330.

Organism-specific databases

TAIRiAT3G20330.

Phylogenomic databases

eggNOGiENOG410IUMU. Eukaryota.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiP49077.
KOiK00609.
OMAiKQSFYGV.
OrthoDBiEOG09360G1O.
PhylomeDBiP49077.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BioCyciARA:AT3G20330-MONOMER.
MetaCyc:AT3G20330-MONOMER.

Miscellaneous databases

PROiP49077.

Gene expression databases

GenevisibleiP49077. AT.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRB_ARATH
AccessioniPrimary (citable) accession number: P49077
Secondary accession number(s): Q683J9, Q94A47, Q9LTR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 11, 2001
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.