Aspartate carbamoyltransferase, chloroplastic precursor - Arabidopsis thaliana (Mouse-ear cress)

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P49077 (PYRB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase, chloroplastic
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	PYRB
Ordered Locus Names:	At3g20330
ORF Names:	MQC12.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
Enzyme regulation	Allosterically regulated by UMP.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.
Subunit structure	Homotrimer Potential.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro cellular response to phosphate starvation Inferred from expression pattern. Source: TAIR pyrimidine ribonucleotide biosynthetic process Inferred from genetic interaction Ref.1. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from genetic interaction Ref.1. Source: TAIR protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Chloroplast Potential

Chain

? – 390

Aspartate carbamoyltransferase, chloroplastic

PRO_0000020348

Experimental info

Sequence conflict

330

Y → F in CAA50687. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P49077 [UniParc].

Last modified July 11, 2001. Version 2.
Checksum: EE8C9216629E826E
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FASTA

390

43,167

        10         20         30         40         50         60 
MSIASSLTSA TLCGASVFPK ALACSSEFPI NLPSPFESSK ICLTSFPASR DLKKNATLNL 

        70         80         90        100        110        120 
TRNVGPVRCH AMQAGTRELK KFELSDVIEG KQFDREMLSA IFDVAREMEK IEKSSSQSEI 

       130        140        150        160        170        180 
LKGYLMATLF YEPSTRTRLS FESAMKRLGG EVLTTENARE FSSAAKGETL EDTIRTVEGY 

       190        200        210        220        230        240 
SDIIVMRHFE SGAARKAAAT ANIPVINAGD GPGEHPTQAL LDVYTIQSEI GKLDGISVAL 

       250        260        270        280        290        300 
VGDLANGRTV RSLAYLLAKF KDVKIYFVSP EIVKMKDDIK DYLTSSGVEW EESSDLMEVA 

       310        320        330        340        350        360 
SKCDVVYQTR IQRERFGERL DLYEAARGKY IVDKDLLGVM QKKAIIMHPL PRLDEITADV 

       370        380        390 
DADPRAAYFR QAKNGLFIRM ALLKLLLVGW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Heterospecific cloning of Arabidopsis thaliana cDNAs by direct complementation of pyrimidine auxotrophic mutants of Saccharomyces cerevisiae. I. Cloning and sequence analysis of two cDNAs catalysing the second, fifth and sixth steps of the de novo pyrimidine biosynthesis pathway." Nasr F., Bertauche N., Dufour M.E., Minet M., Lacroute F. Mol. Gen. Genet. 244:23-32(1994) [PubMed: 8041358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71843 mRNA. Translation: CAA50687.1. AB024036 Genomic DNA. Translation: BAB02813.1. CP002686 Genomic DNA. Translation: AEE76365.1.
IPI	IPI00518176.
PIR	S46441.
RefSeq	NP_188668.1. NM_112924.3.
UniGene	At.20992.
3D structure databases
ProteinModelPortal	P49077.
SMR	P49077. Positions 81-389.
ModBase	Search...
Protein-protein interaction databases
IntAct	P49077. 1 interaction.
STRING	P49077.
Proteomic databases
PRIDE	P49077.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G20330.1; AT3G20330.1; AT3G20330.
GeneID	821577.
GenomeReviews	Gene locus AT3G20330 in contig BA000014_GR.
KEGG	ath:AT3G20330.
Organism-specific databases
TAIR	At3g20330.
Phylogenomic databases
eggNOG	KOG0370.
HOGENOM	HBG579429.
InParanoid	P49077.
OMA	DLYTIRQ.
PhylomeDB	P49077.
ProtClustDB	PLN02527.
Gene expression databases
ArrayExpress	P49077.
Genevestigator	P49077.
GermOnline	AT3G20330. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_ARATH

Accession

Primary (citable) accession number: P49077
Secondary accession number(s): Q9LTR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	July 11, 2001
Last modified:	November 16, 2011
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents