Reviewed, UniProtKB/Swiss-Prot P49077 (PYRB_ARATH)

Names and origin

Protein names

Recommended name:
    Aspartate carbamoyltransferase, chloroplastic
    EC=2.1.3.2
Alternative name(s):
    Aspartate transcarbamylase
      Short name=ATCase

Gene names

Name:	PYRB
Ordered Locus Names:	At3g20330
ORF Names:	MQC12.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
Enzyme regulation	Allosterically regulated by UMP.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.
Subunit structure	Homotrimer Potential.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine ribonucleotide biosynthetic process Ref.1 Inferred from genetic interaction. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Ref.1 Inferred from genetic interaction. Source: TAIR protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 390		Aspartate carbamoyltransferase, chloroplastic		PRO_0000020348
Experimental info
	Sequence conflict	330	1	Y → F in CAA50687. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P49077-1 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: EE8C9216629E826E	FASTA	390	43,167
	10 20 30 40 50 60 MSIASSLTSA TLCGASVFPK ALACSSEFPI NLPSPFESSK ICLTSFPASR DLKKNATLNL 70 80 90 100 110 120 TRNVGPVRCH AMQAGTRELK KFELSDVIEG KQFDREMLSA IFDVAREMEK IEKSSSQSEI 130 140 150 160 170 180 LKGYLMATLF YEPSTRTRLS FESAMKRLGG EVLTTENARE FSSAAKGETL EDTIRTVEGY 190 200 210 220 230 240 SDIIVMRHFE SGAARKAAAT ANIPVINAGD GPGEHPTQAL LDVYTIQSEI GKLDGISVAL 250 260 270 280 290 300 VGDLANGRTV RSLAYLLAKF KDVKIYFVSP EIVKMKDDIK DYLTSSGVEW EESSDLMEVA 310 320 330 340 350 360 SKCDVVYQTR IQRERFGERL DLYEAARGKY IVDKDLLGVM QKKAIIMHPL PRLDEITADV 370 380 390 DADPRAAYFR QAKNGLFIRM ALLKLLLVGW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Heterospecific cloning of Arabidopsis thaliana cDNAs by direct complementation of pyrimidine auxotrophic mutants of Saccharomyces cerevisiae. I. Cloning and sequence analysis of two cDNAs catalysing the second, fifth and sixth steps of the de novo pyrimidine biosynthesis pathway." Nasr F., Bertauche N., Dufour M.E., Minet M., Lacroute F. Mol. Gen. Genet. 244:23-32(1994) [PubMed: 8041358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71843 mRNA. Translation: CAA50687.1. AB024036 Genomic DNA. Translation: BAB02813.1.
IPI	IPI00518176.
PIR	S46441.
RefSeq	NP_188668.1.
UniGene	At.20992 Rra.17541
3D structure databases
SMR	P49077. Positions 85-387.
ModBase	Search...
Protein-protein interaction databases
STRING	P49077.
Proteomic databases
PRIDE	P49077.
Genome annotation databases
GeneID	821577.
GenomeReviews	Gene locus AT3G20330 in contig BA000014_GR.
KEGG	ath:AT3G20330.
Organism-specific databases
TAIR	At3g20330.
Phylogenomic databases
eggNOG	KOG0370.
HOGENOM	HBG579429.
InParanoid	P49077.
OMA	QSFYGVP.
PhylomeDB	P49077.
Enzyme and pathway databases
BRENDA	2.1.3.2. 302.
Gene expression databases
ArrayExpress	P49077.
Genevestigator	P49077.
GermOnline	AT3G20330. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_ARATH

Accession

Primary (citable) accession number: P49077
Secondary accession number(s): Q9LTR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	July 11, 2001
Last modified:	February 9, 2010
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)