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P49075

- GUX3_AGABI

UniProt

P49075 - GUX3_AGABI

Protein

Exoglucanase 3

Gene

cel3

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Shows enzymatic activity towards crystalline cellulose. At long reaction times. It is also able to degrade carboxymethyl cellulose and barley B-glucan.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei215 – 2151Proton donorPROSITE-ProRule annotation
    Active sitei393 – 3931NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17626.

    Protein family/group databases

    CAZyiCBM1. Carbohydrate-Binding Module Family 1.
    GH6. Glycoside Hydrolase Family 6.
    mycoCLAPiCBH6C_AGABI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exoglucanase 3 (EC:3.2.1.91)
    Alternative name(s):
    1,4-beta-cellobiohydrolase 3
    Exocellobiohydrolase 3
    Gene namesi
    Name:cel3
    OrganismiAgaricus bisporus (White button mushroom)
    Taxonomic identifieri5341 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 438418Exoglucanase 3PRO_0000007902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 45By similarity
    Disulfide bondi39 ↔ 55By similarity
    Disulfide bondi170 ↔ 229By similarity
    Disulfide bondi360 ↔ 407By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP49075.
    SMRiP49075. Positions 25-56, 86-438.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 5939CBM1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni60 – 8728LinkerAdd
    BLAST
    Regioni88 – 438351CatalyticAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OMAiKCANAQS.

    Family and domain databases

    Gene3Di3.20.20.40. 1 hit.
    InterProiIPR016288. Beta_cellobiohydrolase.
    IPR000254. Cellulose-bd_dom_fun.
    IPR001524. Glyco_hydro_6_CS.
    [Graphical view]
    PfamiPF00734. CBM_1. 1 hit.
    PF01341. Glyco_hydro_6. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
    PRINTSiPR00733. GLHYDRLASE6.
    ProDomiPD001821. CBD_fun. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00236. fCBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF51989. SSF51989. 1 hit.
    SSF57180. SSF57180. 1 hit.
    PROSITEiPS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
    PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P49075-1 [UniParc]FASTAAdd to Basket

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    MFKFAALLAL ASLVPGFVQA QSPVWGQCGG NGWTGPTTCA SGSTCVKQND    50
    FYSQCLPNNQ APPSTTTQPG TTPPATTTSG GTGPTSGAGN PYTGKTVWLS 100
    PFYADEVAQA AADISNPSLA TKAASVAKIP TFVWFDTVAK VPDLGGYLAD 150
    ARSKNQLVQI VVYDLPDRDC AALASNGEFS LANDGLNKYK NYVDQIAAQI 200
    KQFPDVSVVA VIEPDSLANL VTNLNVQKCA NAQSAYKEGV IYAVQKLNAV 250
    GVTMYIDAGH AGWLGWPANL SPAAQLFAQI YRDAGSPRNL RGIATNVANF 300
    NALRASSPDP ITQGNSNYDE IHYIEALAPM LSNAGFPAHF IVDQGRSGVQ 350
    NIRDQWGDWC NVKGAGFGQR PTTNTGSSLI DAIVWVKPGG ECDGTSDNSS 400
    PRFDSHCSLS DAHQPAPEAG TWFQAYFETL VANANPAL 438
    Length:438
    Mass (Da):46,210
    Last modified:February 1, 1996 - v1
    Checksum:i002C973544893794
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti133 – 1331V → T.
    Natural varianti152 – 1521R → Q.
    Natural varianti244 – 2441V → I.
    Natural varianti248 – 2481N → D.
    Natural varianti398 – 3981N → S.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24519 mRNA. Translation: AAA50607.1.
    L24520 mRNA. Translation: AAA50608.1.
    Z34007 Genomic DNA. Translation: CAA83971.1.
    PIRiS70602.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24519 mRNA. Translation: AAA50607.1 .
    L24520 mRNA. Translation: AAA50608.1 .
    Z34007 Genomic DNA. Translation: CAA83971.1 .
    PIRi S70602.

    3D structure databases

    ProteinModelPortali P49075.
    SMRi P49075. Positions 25-56, 86-438.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM1. Carbohydrate-Binding Module Family 1.
    GH6. Glycoside Hydrolase Family 6.
    mycoCLAPi CBH6C_AGABI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OMAi KCANAQS.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-17626.

    Family and domain databases

    Gene3Di 3.20.20.40. 1 hit.
    InterProi IPR016288. Beta_cellobiohydrolase.
    IPR000254. Cellulose-bd_dom_fun.
    IPR001524. Glyco_hydro_6_CS.
    [Graphical view ]
    Pfami PF00734. CBM_1. 1 hit.
    PF01341. Glyco_hydro_6. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001100. Beta_cellobiohydrolase. 1 hit.
    PRINTSi PR00733. GLHYDRLASE6.
    ProDomi PD001821. CBD_fun. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00236. fCBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51989. SSF51989. 1 hit.
    SSF57180. SSF57180. 1 hit.
    PROSITEi PS00562. CBM1_1. 1 hit.
    PS51164. CBM1_2. 1 hit.
    PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
    PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source."
      Chow C.-M., Yague E., Raguz S., Wood D.A., Thurston C.F.
      Appl. Environ. Microbiol. 60:2779-2785(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 255-277 AND 331-351.
      Strain: D649.
    2. "Correlation of exons with functional domains and folding regions in a cellulase from Agaricus bisporus."
      Yague E., Chow C.-M., Challen M.P., Thurston C.F.
      Curr. Genet. 30:56-61(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Mycelium.

    Entry informationi

    Entry nameiGUX3_AGABI
    AccessioniPrimary (citable) accession number: P49075
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3