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Protein

Exoglucanase 3

Gene

cel3

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Shows enzymatic activity towards crystalline cellulose. At long reaction times. It is also able to degrade carboxymethyl cellulose and barley B-glucan.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215Proton donorPROSITE-ProRule annotation1
Active sitei393NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17626.

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH6. Glycoside Hydrolase Family 6.
mycoCLAPiCBH6C_AGABI.

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase 3 (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase 3
Exocellobiohydrolase 3
Gene namesi
Name:cel3
OrganismiAgaricus bisporus (White button mushroom)
Taxonomic identifieri5341 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000790221 – 438Exoglucanase 3Add BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 45By similarity
Disulfide bondi39 ↔ 55By similarity
Disulfide bondi170 ↔ 229By similarity
Disulfide bondi360 ↔ 407By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP49075.
SMRiP49075.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 59CBM1PROSITE-ProRule annotationAdd BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 87LinkerAdd BLAST28
Regioni88 – 438CatalyticAdd BLAST351

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

OMAiGWPANIQ.

Family and domain databases

Gene3Di3.20.20.40. 1 hit.
InterProiView protein in InterPro
IPR016288. Beta_cellobiohydrolase.
IPR000254. Cellulose-bd_dom_fun.
IPR001524. Glyco_hydro_6_CS.
PANTHERiPTHR34876. PTHR34876. 1 hit.
PfamiView protein in Pfam
PF00734. CBM_1. 1 hit.
PF01341. Glyco_hydro_6. 1 hit.
PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSiPR00733. GLHYDRLASE6.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001821. CBD_fun. 1 hit.
SMARTiView protein in SMART
SM00236. fCBD. 1 hit.
SUPFAMiSSF51989. SSF51989. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEiView protein in PROSITE
PS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49075-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKFAALLAL ASLVPGFVQA QSPVWGQCGG NGWTGPTTCA SGSTCVKQND
60 70 80 90 100
FYSQCLPNNQ APPSTTTQPG TTPPATTTSG GTGPTSGAGN PYTGKTVWLS
110 120 130 140 150
PFYADEVAQA AADISNPSLA TKAASVAKIP TFVWFDTVAK VPDLGGYLAD
160 170 180 190 200
ARSKNQLVQI VVYDLPDRDC AALASNGEFS LANDGLNKYK NYVDQIAAQI
210 220 230 240 250
KQFPDVSVVA VIEPDSLANL VTNLNVQKCA NAQSAYKEGV IYAVQKLNAV
260 270 280 290 300
GVTMYIDAGH AGWLGWPANL SPAAQLFAQI YRDAGSPRNL RGIATNVANF
310 320 330 340 350
NALRASSPDP ITQGNSNYDE IHYIEALAPM LSNAGFPAHF IVDQGRSGVQ
360 370 380 390 400
NIRDQWGDWC NVKGAGFGQR PTTNTGSSLI DAIVWVKPGG ECDGTSDNSS
410 420 430
PRFDSHCSLS DAHQPAPEAG TWFQAYFETL VANANPAL
Length:438
Mass (Da):46,210
Last modified:February 1, 1996 - v1
Checksum:i002C973544893794
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti133V → T. 1
Natural varianti152R → Q. 1
Natural varianti244V → I. 1
Natural varianti248N → D. 1
Natural varianti398N → S. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24519 mRNA. Translation: AAA50607.1.
L24520 mRNA. Translation: AAA50608.1.
Z34007 Genomic DNA. Translation: CAA83971.1.
PIRiS70602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24519 mRNA. Translation: AAA50607.1.
L24520 mRNA. Translation: AAA50608.1.
Z34007 Genomic DNA. Translation: CAA83971.1.
PIRiS70602.

3D structure databases

ProteinModelPortaliP49075.
SMRiP49075.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH6. Glycoside Hydrolase Family 6.
mycoCLAPiCBH6C_AGABI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OMAiGWPANIQ.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17626.

Family and domain databases

Gene3Di3.20.20.40. 1 hit.
InterProiView protein in InterPro
IPR016288. Beta_cellobiohydrolase.
IPR000254. Cellulose-bd_dom_fun.
IPR001524. Glyco_hydro_6_CS.
PANTHERiPTHR34876. PTHR34876. 1 hit.
PfamiView protein in Pfam
PF00734. CBM_1. 1 hit.
PF01341. Glyco_hydro_6. 1 hit.
PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSiPR00733. GLHYDRLASE6.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001821. CBD_fun. 1 hit.
SMARTiView protein in SMART
SM00236. fCBD. 1 hit.
SUPFAMiSSF51989. SSF51989. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEiView protein in PROSITE
PS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGUX3_AGABI
AccessioniPrimary (citable) accession number: P49075
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 15, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.