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P49075

- GUX3_AGABI

UniProt

P49075 - GUX3_AGABI

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Protein

Exoglucanase 3

Gene
cel3
Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Shows enzymatic activity towards crystalline cellulose. At long reaction times. It is also able to degrade carboxymethyl cellulose and barley B-glucan.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151Proton donor By similarity
Active sitei393 – 3931Nucleophile By similarity

GO - Molecular functioni

  1. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
  2. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17626.

Protein family/group databases

CAZyiCBM1. Carbohydrate-Binding Module Family 1.
GH6. Glycoside Hydrolase Family 6.
mycoCLAPiCBH6C_AGABI.

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase 3 (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase 3
Exocellobiohydrolase 3
Gene namesi
Name:cel3
OrganismiAgaricus bisporus (White button mushroom)
Taxonomic identifieri5341 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 438418Exoglucanase 3PRO_0000007902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 45 By similarity
Disulfide bondi39 ↔ 55 By similarity
Disulfide bondi170 ↔ 229 By similarity
Disulfide bondi360 ↔ 407 By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP49075.
SMRiP49075. Positions 25-56, 86-438.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 5939CBM1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 8728LinkerAdd
BLAST
Regioni88 – 438351CatalyticAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

OMAiKCANAQS.

Family and domain databases

Gene3Di3.20.20.40. 1 hit.
InterProiIPR016288. Beta_cellobiohydrolase.
IPR000254. Cellulose-bd_dom_fun.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]
PfamiPF00734. CBM_1. 1 hit.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSiPR00733. GLHYDRLASE6.
ProDomiPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMiSSF51989. SSF51989. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEiPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P49075-1 [UniParc]FASTAAdd to Basket

« Hide

MFKFAALLAL ASLVPGFVQA QSPVWGQCGG NGWTGPTTCA SGSTCVKQND    50
FYSQCLPNNQ APPSTTTQPG TTPPATTTSG GTGPTSGAGN PYTGKTVWLS 100
PFYADEVAQA AADISNPSLA TKAASVAKIP TFVWFDTVAK VPDLGGYLAD 150
ARSKNQLVQI VVYDLPDRDC AALASNGEFS LANDGLNKYK NYVDQIAAQI 200
KQFPDVSVVA VIEPDSLANL VTNLNVQKCA NAQSAYKEGV IYAVQKLNAV 250
GVTMYIDAGH AGWLGWPANL SPAAQLFAQI YRDAGSPRNL RGIATNVANF 300
NALRASSPDP ITQGNSNYDE IHYIEALAPM LSNAGFPAHF IVDQGRSGVQ 350
NIRDQWGDWC NVKGAGFGQR PTTNTGSSLI DAIVWVKPGG ECDGTSDNSS 400
PRFDSHCSLS DAHQPAPEAG TWFQAYFETL VANANPAL 438
Length:438
Mass (Da):46,210
Last modified:February 1, 1996 - v1
Checksum:i002C973544893794
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti133 – 1331V → T.
Natural varianti152 – 1521R → Q.
Natural varianti244 – 2441V → I.
Natural varianti248 – 2481N → D.
Natural varianti398 – 3981N → S.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24519 mRNA. Translation: AAA50607.1.
L24520 mRNA. Translation: AAA50608.1.
Z34007 Genomic DNA. Translation: CAA83971.1.
PIRiS70602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24519 mRNA. Translation: AAA50607.1 .
L24520 mRNA. Translation: AAA50608.1 .
Z34007 Genomic DNA. Translation: CAA83971.1 .
PIRi S70602.

3D structure databases

ProteinModelPortali P49075.
SMRi P49075. Positions 25-56, 86-438.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM1. Carbohydrate-Binding Module Family 1.
GH6. Glycoside Hydrolase Family 6.
mycoCLAPi CBH6C_AGABI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OMAi KCANAQS.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17626.

Family and domain databases

Gene3Di 3.20.20.40. 1 hit.
InterProi IPR016288. Beta_cellobiohydrolase.
IPR000254. Cellulose-bd_dom_fun.
IPR001524. Glyco_hydro_6_CS.
[Graphical view ]
Pfami PF00734. CBM_1. 1 hit.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view ]
PIRSFi PIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSi PR00733. GLHYDRLASE6.
ProDomi PD001821. CBD_fun. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00236. fCBD. 1 hit.
[Graphical view ]
SUPFAMi SSF51989. SSF51989. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEi PS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source."
    Chow C.-M., Yague E., Raguz S., Wood D.A., Thurston C.F.
    Appl. Environ. Microbiol. 60:2779-2785(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 255-277 AND 331-351.
    Strain: D649.
  2. "Correlation of exons with functional domains and folding regions in a cellulase from Agaricus bisporus."
    Yague E., Chow C.-M., Challen M.P., Thurston C.F.
    Curr. Genet. 30:56-61(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Mycelium.

Entry informationi

Entry nameiGUX3_AGABI
AccessioniPrimary (citable) accession number: P49075
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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